Objective To explore the clinical value of detecting T cell subsets,immunoglobulin(Ig) ,erythro-cyte sedimentation rate(ESR),C-reactive protein(CRP) in patients with juvenile rheumatoid arthritis(JRA). Meth-ods T cell subsets(CD3+ , CD4+ , CD8+ , CD16+CD56+) was detected with flow cytometer, Ig, CRP with rate nephelome-try,ESR with Westergren's method in 40 cases of JRA,and other 40 normal children were served as normal control group. Results The level of CD3+ ,CD8+ ,CD16+CD56+ in the JRA group were lower,but CD4+ was higher than those in normal control group(P< 0.05 all) ;the level of IgA, IgG, IgM, ESR, CRP in the JRA group were all higher than those in the normal control group( P < 0.05 ). Conclusion There is an inflammation in patients with JRA, and when disease activity there is an immunological aberrance with cellular and humoral immunization.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

BACKGROUND: Breviscapine, extracted from a Chinese herb Erigeron breviscapus (Vant.) Hand-Mazz (Bre), has remarkable activities against platelets and thrombus formation. It can protect against ischemic brain damage through eliminating free radicals and apoptosis.OBJECTIVE: To study the protective effects of Breviscapine on brain damage in rats after ischemia-reperfusion injury, taking MgSO4 as control.DESIGN: Randomized controlled study and analysis of variance.SETTING: College of Life Science and Technology, Xiaogan University.MATERIALS: This experiment was conducted in the College of Life Science and Technology of Xiaogan University between May 2004 and November 2004. Forty male Wistar rats were selected and randomized into five groups: sham-operation group, cerebral ischemia-reperfusion (IR)group, MgSO4 group, 50 mg/kg Breviscapine group and 75 mg/kg Breviscapine group with 8 in each group.METHODS: Rats were subjected to cerebral ischemia-reperfusion induced by inserting a nylon thread into the internal carotid artery to block the origin of middle cerebral artery and removing the thread later. Normal saline of 20 mL/kg was administrated in sham-operation group and IR group, while 50 mg/kg, 75 mg/kg Breviscapine and 30 mg/kg MgSO4 were administrated in other groups respectively 10 minutes after the onset of ischemia. Neurological scoring was performed 1 hour, 2 hours, 5 hours and 23 hours after cerebral ischemia-reperfusion (Five points in total: 0 as unobvious neurological symptom; 1 as inability to completely extend left anterior claw; 2 as rotation toward the left; 3 as inclining to the left side atwalking; 4 as inability to walk by itself. The higher scale, the more severe the behavioral disturbance was). Brain infarcted area was assayed 1 hour and 23 hours after cerebral ischemia-reperfusion, and was expressed as the percentage of stained area to non-stained area. Neuron apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTPfluorescence nick end labeling (TUNEL) method to assess DNA fragments of apoptotic cells 1 hour after cerebral ischemia, and 2 hours, 5 hours and 23 hours after reperfusion. The apoptotic cells were counted under the fluorescence microscope. The percentage of apoptosis cells (%) = (apoptotic neurons ÷ hippocampal neurons) ×100%. The protein expression of caspase-3 was detected by immunohistochemical staining. The positive cells were identified and counted under the light microscope. The percentage of positive cells of caspase-3 (%) = (positive neurons of caspase-3 ÷ hippocampal neurons) ×100%.cells of caspase-3 1 hour after cerebral ischemia, and 2, 5 and 23 hours after reperfusion in each group.scoring: Neurological score of rats in 50 mg/kg, 75 mg/kg Breviscapine groups and MgSO4 group was 1.2±0.4, 0.5±0.4, and 1.3±0.4, respectively,which were all lower than that in IR group [(2.2±0.6)] 23 hours after cerebral ischemia-recirculation (F=6.09, P=0.001). However, it was lower in farcted area in rats of 50 mg/kg and 75 mg/kg Breviscapine groups and MgSO4 group was (0.18±0.03)%, (0.10±0.02)%, and (0.28±0.02)%, respectively, which were all lower than that of IR group [(0.43±0.05) %] 23 hours after cerebral ischemia-reperfusion (F=2.3, P=0.001). It was smaller in hippocampal apoptotic cells: It was (27.2±4.3) %, (20.6±3.6)%, and (35.4±5.5)% in 50 mg/kg and 75 mg/kg Breviscapine groups and MgSO4 group,which were all lower than that of IR group [(60.4±6.2)%] 23 hours after cerebral ischemia-reperfusion (F=6.17, P=0.000 7). It was lower in Brevispositive cells of caspase-3: It was (34.2±5.3)%, (21.6±3.5)%, and (47.4±4.5)% in 50 mg/kgand 75 mg/kg Breviscapine groups and MgSO4 group,which were all lower than that of IR group [(76.3±6.2)%] 23 hours after cerebral ischenia-reperfusion (F=6.88, P=0.000 1). It was lower in Breviscapine group than in MgSO4 group (P ＜ 0.01).CONCLUSION: Breviscapine can protect brain damage induced by cerebral ischemia-reperfusion by markedly decreasing the neurological score,area of cerebral infarction, number of hippocampal apoptotic cells and number of positive cells of caspase-3, and it has better effect than MgSO4.

Objective To investigate the effect of cerebroprotein hydrolysate on the serum high sensitivity C reactive protein (hsCRP), insulin-like growth factor-1 (IGF-1) and interleukin-18 (IL-18) in patients with hypoxic ischemic encephalopathy (HIE) in plateau region. Methods From January, 2013 to May, 2015, 104 cases with neonatal HIE in our department were randomly divided into conventional treat-ment group (n=52) and cerebroprotein hydrolysate group (n=52). Besides, 35 cases of healthy newborn were chosen as the control group. The total effective rate and the serum hsCRP, IGF-1, IL-18 levels were compared. Results The IGF-1 level was lower, and the levels of hsCRP and IL-18 were higher in moderate HIE group and severe HIE group than in the control group and the mild HIE group, respectively (P<0.05). The efficiency rate was significantly higher in the cerebroprotein hydrolysate group than in the conventional treatment group (χ2=8.922, P=0.012). Compared with the conventional treatment group, the IGF-1 level increased, and the levels of hsCRP and IL-18 decreased in the cerebroprotein hydrolysate group (P<0.05). Conclusion Cerebroprotein hydrolysate is effective on patients with HIE in plateau re-gion. The changes of serum hsCRP, IGF-1, and IL-18 levels can be used as auxiliary indexes for early diagnosis of HIE.

Objective To discuss the influence of the management of family-doctor responsibility system on drug utilization and compliance of hypertensive patients.Methods 1039 primary hypertension patients in Zhenhai Lianhua Community who were under the management of the residents electronic health files in June 2012,were implemented with the latest family-doctor responsibility system for eight months.The on-site questionnaire survey and electronic health management platform were carried out to analyze the drug utilization and treatment compliance of hypertensive patients.Results The proportion of taking medicine treatment among 1039 patients with essential hypertension reached 85.2％,including 56.3％ patients who took one kind of drug,35.4％ patients who took two kinds of drugs and 8.3％ patients who took at least three kinds of drugs.The most commonly used drugs were Amlodipine Besylate,Felodipine,Irbesartan and Hydrochlorothiazide tablets,Irbesartan,Levamlodipine Besylate tablets,Metoprolol Tartrate tablets and etc.The rate of patient compliance was 65.5％.After comparing the compliance of any two among three kinds of drug groups,the comparison of compliance between patients who took one kind,two or three kinds of drugs showed statistical significance.The comparison of drug types and compliance showed that compliance with compound preparation was statistically different from compliance with ARB,calcium antagonists,sympatholytic agent,diuretics.The educational level was also related to the compliance.Conclusions After the implementation of the family-doctor responsible management,the treatment rate and compliance rate are high,and the patients with hypertension in community still give priority to long-acting drugs.

In broad definition,diseases caused by exogenous pathogenic factors involve with respiratory system,digestive system,urinary system,and nervous system;While concerned with internal medicine of respiration,they are common cold,upper respiratory tract infection,acute tracheitis and bronchitis.Clinically,we call diseases that caused by external pathogens,showing symptoms of respiratory system in general,as FeiXi Waigan Bing(lung system diseases caused by external pathogens).In curing these diseases,we integrated traditional Chinese and western medicine and got satisfactory clinical effect.This demonstrated the advantages and features of Traditional Chinese medicine in curing lung system diseases caused by external pathogens.

Patients with congenital cataract often present strabismus simultaneously.Strabismus is closely related to the occurrence of cataract, and is one of the main threats for the development of binocularity and the therapeutic effect of amblyopia.In addition, strabismus negatively affects the appearance and psychological well-being of patients.The influencing factors of strabismus associated with congenital cataract include bilateral or unilateral congenital cataract, type of cataract, timing of cataract surgery, cataract surgery techniques, type of optical correction of aphakia and so on.Visual rehabilitation after congenital cataract surgery is inseparable from the treatment of strabismus.In this article, recent progress on the incidence, pathogenesis, influencing factors, timing of surgery and prognosis of strabismus associated with congenital cataract were reviewed, which might help to understand its clinical features and relevant treatment strategy.

As the medical health service is closely related to health of general public,making harmonious hospital inevitably becomes an important link in constructing a harmonious society.The mutual trust is the most fundamental and active factor in physician-patient relationship.Therefore,construction of a harmonious hospital in the new situation should embark from the hospital connotation construction in great details to reconstruct a harmonious medical relation with mutual trust between doctors and patients.

Objective To investigate the effects of all-trans-retinoic acid(ATRA) on proliferation of cultured human retinal pigment epithelium(RPE) cells and the probable mechanisms.Methods Cultured human RPE cells were treated with various concentrations(10-9,10-8,10-7,10-6 and 10-5 mol?L-1) of ATRA at different time points(6,12,24,48,72 and 96 h).Cell proliferation was evaluated by cell count and MTT colorimetric assay,and cell cycle analysis was performed by flow cytometry.Results The cell viability rates of ATRA treated group were decreased obviously,compared with control groups(P

The pharmacokinetics of Luteolin ( Lut ) solution in rabbits and rats was investigated. Lut in plasma, bile and urine as well as protocatechuic acid (which is a product of Lut) in plasma and urine were determined by fluorescence spectrophotometry. A curve with multiple peaks was fitted to the data of plasma concentration versus time, employing a nonlinear program developed with an IBM-PC computer. The results indicated that the data set obtained with 30 mg /kg doses by iv fitted well to a peculiar open 3 compartment model involved in the enterohepatic recirculation of the drug and the undulations of the drug concentration.The essential pharmacokinetics parameters (mean?SD) were as follows; The t1/2 values for the ?-phase and ?-phase were about 0.18?0.01 h and 1.66?0.24 h respectively. The apparent volume of distribution ( Vd) was about 1.43? 0.45 L?kg-1. The reabsorption rate constant ( Ka ) was 0.465? 0.08 h-1 The reabsorption percent ( F' ) was about 6.9? 2.1% . The elimination rate constant was 1.124?0.35 h-1. The clearance was 588?113 ?g?h-1?kg-1. This study indicated that Lut was rapidly removed from the blood by both kidney and liver where Lut was excreted by way of the bile into intestine from which it Was reabsorbed. The 12 h urine excretion of Lut in rabbits was in the order of 37.7% of total iv dose. In rats, the 6 h bile excretion of Lut was about 11.2% of that total iv dose. A comparison of the C-T curve of PCA with of Lut suggested that PCA was the one of the products of Lut.In rats, the 3H-Lut orally administered was widely distributed in the tissues, the concentrations being higher in liver and kidney.