Objective:To analyze the curative effects of laparoscopic radical prostatectomy for the prostate cancer and the its effect on the level serum hormones levels.Methods:86 patients with prostate cancer were selected and divided into the control group and the observation group with 43 cases in each group according to the draw method.The control group was treated by open radical prostatectomy,while the observation group was treated by laparoscopic radical prostatectomy.The operation indicators,serum follicle stimulating hormone (FSH),luteinizing hormone (LH),dihydrotestosterone (DHT),free testosterone (FT),total testosterone (T),prostate specific antigen (T-PSA,F-PSA) levels,CD3+,CD4+,CD8+,CD4+/CD8+ before and after the surgery as well as the occurrence of postoperative complications were compared between two groups.Results:The operation time of observation group was significantly longer than that of the control group,and the blood loss,bowel function recovery time,hospital stay,pain scores of observation group was significantly lower or shorter than those of the control group (P＜0.05).No significant difference was found in the serum FSH,LH,DHT,FT,T,T-PSA,F-PSA levels before and after the surgery between two group(P＞0.05).The CD3+,CD4+,CD4+/CD8+ of observation group was significantly higher than those of the control group (P＜0.05),the CD8+ of observation group was lower than that of the control group(P＜ 0.05).The incidence rate of postoperative complication in the observation group was significantly lower than that of the control group (P＜0.05).Conclusion:Laparoscopic radical prostatectomy could play a similar effect with the open surgery on the tumor control,it could improve the level of serum testosterone,immune function with high safety.

Objective To explore the biological behavior of renal cell carcinoma cases after treatment with siRNA silencing epidermal growth factor receptor (EGFR) gene.Methods Renal cell carcinoma cell line ACHN were divided into three groups,the control group without any intervention,negative control transfection group by adding the nonspecific transfection reagent and siRNA interference,and the observation group adding transfection reagent and siRNA interference.Cells with EGFR expression and proliferation activity,growth curve,migration and invasion activity were detected in three groups.Results After 72 h of siRNA treatment,the observation group had significantly lower EGFR expression levels,and cell growth activity ability,and cell number of RNAi group after 48 h than negative control transfection group and control group (P ＜ 0.05).Compared with the blank control group and negative control transfection group,the observation group was significantly inhibited (P ＜0.05),and cell scratch distance after 12 h and 24 h was significantly higher than that of the control group and negative control transfection group (P ＜ 0.05).The control group and negative control transfection group showed no significant difference in the cell membrane at 12 h (P ＞0.05).The observation group had significantly lower transmembrane cell number than the control group and negative control transfection group (P ＜ 0.05).Conclusion The proliferation,growth,migration and invasion of renal carcinoma cells can be effectively improved by using siRNA EGFR protein.

Objective To explore the biological behavior of renal cell carcinoma cases after treatment with siRNA silencing epidermal growth factor receptor (EGFR) gene.Methods Renal cell carcinoma cell line ACHN were divided into three groups,the control group without any intervention,negative control transfection group by adding the nonspecific transfection reagent and siRNA interference,and the observation group adding transfection reagent and siRNA interference.Cells with EGFR expression and proliferation activity,growth curve,migration and invasion activity were detected in three groups.Results After 72 h of siRNA treatment,the observation group had significantly lower EGFR expression levels,and cell growth activity ability,and cell number of RNAi group after 48 h than negative control transfection group and control group (P ＜ 0.05).Compared with the blank control group and negative control transfection group,the observation group was significantly inhibited (P ＜0.05),and cell scratch distance after 12 h and 24 h was significantly higher than that of the control group and negative control transfection group (P ＜ 0.05).The control group and negative control transfection group showed no significant difference in the cell membrane at 12 h (P ＞0.05).The observation group had significantly lower transmembrane cell number than the control group and negative control transfection group (P ＜ 0.05).Conclusion The proliferation,growth,migration and invasion of renal carcinoma cells can be effectively improved by using siRNA EGFR protein.

Objective To analyze the four types of laparoscopic operation in the treatment of upper ureteral calculi with diameter more than 1 cm.Methods From July 2012 to September 2015,150 cases of patients with upper ureteral calculi diameter more than 1cm were analyzed.According to the different surgical methods,patients were divided into four groups.There are 39 cases in URL group,32 cases in RLU group,36 cases in PCNL group and 43 cases in FURL group.The diameter of calculi in RLU group and PCNL group was much larger than those in URL group and FURL group(P ＜ 0.05).There was no difference in terms of sex,age,stone location,stone composition,and the ESWL treatment failure rate among the four groups(P ＞ 0.05).The operation time,postoperative hospitalization days,postoperative bowel recovery,postoperative ESWL rate,complications and postoperative calculi clearance rate were compared among the four groups.Results In RLU,PCNL,URL and FURL group,the operation time were (38.5 ± 13.0) min,(83.4 ± 6.1) min,(69.6 ± 13.0) min and (50.1 ± 6.3) min,respectively,and there were significant differences among the four groups (RLU group ＞ PCNL group ＞ FURL group ＞ URL group,P ＜ 0.05).Postoperative hospitalization days of RLU group,PCNL group,URL group and FURL group were (6.5 ± 0.6) d,(9.5 ± 0.7) d,(8.4 ± 0.7) d and (7.7 ± 0.7) d respectively,and there were significant differences in terms of hospitalization days (RLU group ＞ PCNL group ＞ FURL group ＞ URL group,P ＜ 0.05).The calculi clearance rates of RLU group,PCNL group,URL group and FURL group were 79.5％,100.0％,94.4％ and 88.4％ at 3 days after operation,and 82.1％,100.0％,97.2％ and 95.3％ at 1 month after operation.The postoperative calculi clearance rates at 3 days and 1 month in RLU group were higher than those in URL group(P ＜ 0.05),but there was no difference between PCNL group and RLU group (P ＞ 0.05).Postoperative ESWL rates of RLU group,PCNL group,URL group and FURL group were 12.8％,0,2.8％ and 4.7％,and there was no difference among the four groups.The decreased value of RBC in PCNL group and FURL group was higher than that of URL group and RLU group(P ＜ 0.05).One patient in PCNL group suffered from massive hemorrhage,and required blood transfusion.The complication rates in the four groups were 23.1％ (9/39),9.4％ (3/32),13.9％ (5/36) and 9.3％ (4/43) respectively,and there was no difference of complication among them(P ＞ 0.05).Conclusions Four types of endoscopes have the advantages of safety,small wound and quick recovery for ureter calculi larger than 1 cm.The URL operation is simple,with less injury,quick recovery,but low stone clearance rate.FURL requires high level of skill for the operator,and is an effective treatment for ureteric,renal pelvic,and kidney stones which are smaller than 2 cm.FURL avoids the extra ESWL treatment,but costs much.PCNL has more injury and requires high-level surgical skills with a high stone clearance rate.RLU has a high stone clearance rate but is much difficult,which can be used as a remedy for the failure of FURL or PCNL.