The chemiluminescence (CL) reaction was observed from the synthesized N-tetrahydrobenzothiazolyl imines mixed with acidified potassium permanganate and the emission intensity was significantly enhanced, when the formic acid was added. The fluorescence spectra, CL spectra, UV-VIS absorption spectra and kinetic property of the reaction were investigated. Based on the CL reaction, a flow injection method for the determination of Ntetrahydrobenzothiazolyl imine was developed. The relative standard deviation for eleven replicate injections of 1.0× 10-6 mol/L schiff bases of N-tetrahydrobenzothiazolyl imine was 1.7%, and the detection limit (3σ) was 5.0 ×10-8 mol/L. Mn2+ ,Fe3+ ,Cu2+ ,Ni2+ ,Cr3+ ,Mg2+ ,Zn2+ ,Tl+ ,Bi2+ and Al3+ ions had no effect whilst Ag+ and Fe2+ ions interfered with the determination.

Objective To provide guidance for oral health prevention by investigating oral health status of 480 Lahu people.Methods Oral health status of 480 Lahu people were investigated by trained dental therapists using standard diagnostic criteria and record.Participants were divided into 4 groups according to their age.Results The Lahu People in Linxiang county suffered from severe dental caries and periodontal disease.The caries prevalence rate among children of 5 years old was 78.3％.The rate of calculus among the children of 12 years old was 75％.The prevalence rates of caries and periodontal pockets were 91.7％ and 43.3％ among the adults between 35 and 44 years old.The above data were significantly higher than the results of the Third National Oral Health Survey.The rates of gingival bleeding and periodontal pocket were 51.7％ and 49.2％ among the aged from 65 to 74 years old,which were lower than that of the Third National Oral Health Survey.Among the participants,76％ never brushed teeth and 85％ brushed teeth only once a day in people who brushed teeth regularly.Conclusion Poor status and maintenance of oral health in the Lahu People suggest that education and resources for oral health should be invested.

Objective To evaluate the effect and safety of trans-arterial chemotherapy embolization (TACE) in the treatment of primary hepatic carcinoma ( PHC ) with portal vein tumor thrombus. Methods There were 83 PHC patients complicated with portal vein tumor thrombus, age ranging from 29 to 76 years. Fifty-three cases underwent TACE ( Group A) , 30 receiving trans-arterial chemotherapy infusion only (TAI, Group B). Results The survival rate in group A at 6,12,24 and 36 months was 97. 8% , 85.2%,45.8% and 4.4%, respectively, significantly higher than that in group B (37. 6% ,5. 3% ,0,0, P

0.05)before treatment.(2)After 6 months' treatment,there was significant difference in PMC and EMC(P0.05).VNDmax and VTmax were significantly lower in group one than in group two(P

Objective To study the effect of the PDCA management on safety management of venous transfusion by indwelling needles?.Method The measures included standardizing nurses manipulation,improving the management system related to venous transfusion,and regulating details management process for tube fixing and dressing and tube maintenance.Results The rates of unstandardized maintenance and obstruction of tubes after the enforcement of details management were significantly lower than those before its enforcement(P<0?05).Conclusions PDCA management is effective for standardizing nursing implementation and ensuring the safety of venous transfusion by indwelling needle?

Objective To analyze the antioxidant activities of extracts from Scutellaria baicalensis Georgi. by different solvents, and to determine the contents of total flavonoids. Methods By employing colorimetric method, contents of total flavonoids in different extracts were determined. The free radical scavenging activity of each extract was measured by DPPH method. Results Different extracts from Scutellaria baicalensis Georgi. by different solvents were provided with DPPH free radical scavenging activities, of which the ethyl alcohol extracts were the highest in scavenging activities. In addition, total flavonoids content of ethyl alcohol extracts was significantly higher than that of others. Conclusion The antioxidant activity of extracts from Scutellaria baicalensis Georgi. was related to the content of total flavonoids in it.

Objective To observe the changes of cardiovascular function after acupuncture at Neiguan (PC 6) respectively with slowly twirling needling and tube needling in healthy subjects, and to objectively evaluate the pain intensity of the two needling methods.Method A hundred healthy young subjects were randomized into group A and group B, 50 cases in each group. Neiguan (PC 6) point was selected for acupuncture in both groups. In the first stage, group A received acupuncture by slowly twirling needling, while group B received acupuncture with tube needling; in the second stage (a week later), group A received acupuncture with tube needling, while group B by slowly twirling needling. The Short-form McGill Pain Questionnaire (SF-MPQ) was observed after acupuncture for each subject, and ZXG-E automatic cardiovascular function detector was adopted to evaluate the cardiovascular function before puncturing, 5 min after puncturing, and 30 min after puncturing.Result The Sensory Pain Rating Index (S-PRI) and total SF-MPQ scores of slowly twirling needling were significantly different from that of tube needling (P<0.01). The Affective Pain Rating Index (A-PRI), Visual Analogue Scale (VAS), and Present Pain Intensity (PPI) of slowly twirling needling method were significantly different from that of tube needling (P<0.01). At 5 min after needle insertion, the FEK and VER values of twirling needling were significantly different from that of tube needling (P<0.01).Conclusion The pain produced by slowly twirling needling is more variant and stronger than that by tube needling, and this pain variation can produce a positive effect on cardiovascular function.

Objective:To explore the feasibility and clinical efficacy of stratified suture of lateral pharyngeal wall(SSLPW) combined with soft palate radiofrequency coblation in the treatment of the patients with severe obstructive sleep apnea hypopnea syndrome(OSAHS).Methods:The clinical data of 21 severe OSAHS patients underwent lateral pharyngoplasty(LP) combined with soft palate radiofrequency coblation (LP group) and 39 severe OSAHS patients underwent SSLPW combined with soft palate radiofrequency coblation (SSLPW group) were selected.The apnea hypopnea index(AHI),lowest SaO2(LSaO2),Epworth sleepiness scale(ESS) scores,and related postoperative complications of the patients in two groups were analyzed before and after operation.The successful rates of operation of the patients in two were compared.Results:In LP group,1 patient was cured (5%),19 patients were improved markedly (90%),1 patient was effective (5%),and no patient was invalid;the successful rate was 95.2%(20/21).In SSLPW group,2 patients were cured (5.1%),33 patients were improved markedly (84.6%),4 patients were effective (10.3%),and no patients was invalid;the successful rate was 89.7%(35/39);there was no statistical difference in the successful rate of operation of the patients between two groups (P>0.05).There was statistically significant improvement of the subjective symptoms.All the patients returned to normal subjective swallowing functions without nasal pharyngeal reflux and dysphagia in one month follow-up after operation.Compared with before operation,the AHI and ESS scores of the patients in two groups after operation were decreased(P<0.05);the LSaO2 scores were increased(P<0.05).Conclusion:The AHI,LSaO2 and ESS scores are significantly improved in the OSAHS patients after treated with SSLPW combined with soft palate radiofrequency coblation.The method is a valid option for the severe OSAHS patients.

Objective To observe the clinical efficacy of moxibustion in treating perimenopausal syndrome (PMS).Method Totally 108 PMS patients of yang deficiency or yin deficiency constitution were randomized into a treatment group of 56 cases and a control group of 52 cases. The treatment group was intervened by moxibustion, while the control group was by medication. The modified Kupperman Index (KI), Hamilton Anxiety Scale (HAMA), Symptom Checklist-90 (SCL-90), and Self-rating Depression Scale (SDS) were observed before and after treatment for comparison.Result The KI score, HAMA score, SCL-90 total score, and SDS score were significantly changed in both groups after intervention (P<0.01,P<0.05). After treatment, the KI score, HAMA score, SCL-90 total score, and SDS score in the treatment group were significantly different from that in the control group (P<0.01,P<0.05).Conclusion Moxibustion is effective in treating PMS, and it can improve the anxiety and depression symptoms of the patients.

In this study, DNAzymes against cyclin D1 (cyclin D1-DRz) were designed according to the secondary structure of cyclin D1 mRNA which was computed with RNAdraw and Mfold. Cyclin D1-DRz were transfected into tumor cell line u251 and HeLa by oligofectamine. The expression of cyclin D1 was detected by RT-PCR. It was shown that the expression of cyclin D1 gene was suppressed obviously, and the expressions of other cell-cycle related genes such as cyclin E1, cyclin A1 and cyclin B1 were also declined. The cell cycle analysis of tumor cells tansfected with cyclin D1-DRz revealed an arrestment in the G0/G1 phase. In conclusion, the approach is effective and feasible for designing DNAzyme. Cyclin D1-DRz is useful for interfering with the cell cycle procession of tumor cells.
Base Sequence ; Cell Cycle ; drug effects ; Cyclin D1 ; antagonists & inhibitors ; genetics ; metabolism ; DNA, Catalytic ; genetics ; metabolism ; pharmacology ; HeLa Cells ; Humans ; Molecular Sequence Data ; RNA, Messenger ; genetics ; metabolism ; Transfection