In vitro cellular adherence of murine neutrophils (Neu), macrophages (Mo) and eosinophils (Eos) to infective larvae (L3) of periodic Brugia malayi was promoted by immune serum (AIMS) or fresh normal mice serun (fNMS). Mo and Neu were more potent than Eos . in killing the L3. Eos could adhere to the L3 in the presence of AIMS or fNMS but a weak cy-totoxic effect was observed only when AIMS was used. The activity in AIMS could be removed with Staphylococcus aureus containing Protein-A (SPA) or anti-mice IgG antiserum suggesting that IgG might be in the observed cytotoxicity. Immunofluorescent staining revealed IgG binding to the surface of L3 after incubation with AIMS. Heat treatment of AIMS at 56℃ 30 min resulted in a reduced cytotoxic effect suggesting the role of complement in the reaction. EDTA but not EGTA abolished this activity indicating the operation of complement by alternate pathway. Scanning electron microscopic (SEM) studies comfirmed that the celluar adherence resulted in gross surface damage to the infective larvae.

Objective To study the characteristics of auditory brainstem response by chirp and click stimuli in normal hearing children.Methods A total of 97 children (194 ears)with normal hearing were recruited,including 50 male (100 ears),and 47 female (94 ears).The CE-chirp and the click acoustic stimulation were used to,and the ABR waveforms and latencies were obtained.ResuIts The chirp-ABR Ⅰ wave recognition rate (36%)was significantly lower than that of click-ABR (100%).At 80 dB nHL,chirp-ABR V wave latency was shorter than click-ABR,the difference was statistically significant (P< 0.05 ).The chirp and click ABR V wave response thresholds showed no significant difference (P>0.05).At 80 dB nHL,no statistical significance of two kinds of a-coustic stimulation of ABR V wave amplitude difference (P>0.05).At 50 dB nHL,chirp-ABR significantly in-creased than click-ABR V wave amplitude with statistically significant differences (P<0.05).At 80,and 50 dB nHL,chirp-ABR showed no differences in the amplitude of V wave (P>0.05 ).ConcIusion The chirp acoustic stimulation produced the larger response amplitude,and was faster,demonstrating the noticeable advantages to tes-ting children.

To investigate the nature of syndrome of traditional Chinese medicine by means of pharmacokinetic (PK) method.

Objective To investigate the effect of secondary transurethral electrocision for recurrent superficial bladder cancer.Methods One hundred and twenty patients with recurrent superficial bladder cancer were divided into experiment group and control group by random mechanical sampling method with 60 cases of each.Experiment group underwent secondary transurethral electrocision at 4 weeks after initial transurethral electrocision and received mitomycin bladder perfusion chemotherapy,control group patients underwent initial transurethral electrocision and received mitomycin bladder perfusion chemotherapy.The two groups' follow-up cystoscopy at 3-month intervals,the follow-up time was 6-34 (18.6 ± 6.5) months,the recurrence was compared between each other.Results Within the follow-up period,the recurrence rate in experiment group was significantly lower than that in control group [21.7％(13/60) vs.63.3％(38/60),P＜0.01].Of all the recurrent patients,high grade rumor (G2 and G3 grade) was mainly,the recurrence rate of high grade tumor in experiment group was significantly lower than that in control group [26.2％ (11/42) vs.70.0％(35/50),P ＜ 0.01].Conclusions Secondary transurethral electrocision can reduce the recurrence rate of superficial bladder cancer (especially for the high grade tumor),it can exactly make the classification of malignant tumors and find residual tumor.So it is valuable in the clinical treatment.