Functional gastrointestinal disease is a group of clinical syndrome of non-organic disease. Its various clinical symptoms have a certain specificity and overlap phenomenon, and the mechanism is not clear. TCM believes thatphychological factorsare an important cause. Emotion failurecan effect spleen and stomach functionthrough liver and heart directly or indirectly.At present, the phychological factors and the relationship between functional gastrointestinal disease are getting attentiongradually. It is recognized that the mechanism of phychological factorsmay be related to brain axis dysfunction, mast cell activation, intestinal flora and so on. This article expounded the above-mentioned mechanism and reviewed the detailed TCM intervention measures to functional gastrointestinal disease in recent years.

Irritable bowel syndrome( IBS)is a common functional gastrointestinal disease,relevant investigations on pathogenesis of IBS mainly focus on genetic susceptibility, social psychological stress, visceral hypersensitivity, dysregulation of brain-gut axis,dysbiosis of intestinal flora,and dysimmunity of intestinal mucosa. This article reviewed the correlation between dysbiosis of intestinal microbiota and dysregulation of brain-gut axis in IBS.

BACKGROUND: The frequency of body ailments combined with depression is relatively higher, and hence post stoke depression(PSD) has gained more and more concentration.OBJECTIVE: To investigate the morbidity, related factors and pathophysiological mechanism of PSD.DESIGN: Case analysis and observation study.SETTING: Department of eurology in a hospital of a university; Third department of internal medicine in a hospital of Chinese People' s Armed Police Force; Department of neurology in a province hospital.PARTICIPANTS: This study was carried out at the Neurological Department of First Clinical Hospital Affiliated to Harbin Medical University and the General Hospital of Armed Police Army in Heilongjiang Province from January 2001 to May 2004. A cohort of 312 stroke patients was followed up in this study.METHODS: All 312 patients scored for Hamilton rating scale for depression (HAMD) and European stroke scale(ESS) within 48 hours of hospitalization. Meanwhile their clinical presentations and social-psychological factors were also collected.MAIN OUTCOME MEASURES: The stroke morbidity in patients with different sex, age, personality, educational degree, as well as stroke type, lesion location and extent of neurological functional deficit.RESULTS: The morbidity of PSD was 40. 1%, predominated by mild and medium stroke; amongst which PSD was found more frequent in women than men( P ＜ 0.01), as well as in elders than youngers( P ＜ 0.01); Patients who has diffidence personality are liable to suffer from PSD; and it was found more common in patients with over middle school education than those received only elementary school education ( P ＜ 0.01 ); depression was more frequent in left-sided stroke than right-sided stroke( P ＜ 0.01 ), especially in frontal lobal stroke; PSD was found negatively correlated with the scores for ESS(r= -0.846, P ＜0.05).CONCLUSION: As the result of the interaction of multiple factors, the development of PSD is found accorded with biology-psychology-society mode.

Objective To observe the changes of plasma neuropeptide Y (NPY) and neurotensin (NT) levels in patients with heart failure and investigate their clinical significance. Methods The levels of plasma NPY, NT of 76 patients with heart failure and 28 normal controls were determined by radioimmunoassay (RIA). The color echocardiogram was used to evaluate the cardiac structure, function and left ventricular ejection fraction (LVEF). Results The level of plasma NPY in patients with heart failure [(159.7 ± 56.3) ng/L] was higher than that in normal controls [(120.8 ± 51.9) ng/L] (P < 0.05), the level of plasma NT [(69.5 ± 29.6) ng/L] was significantly lower than that in normal controls [(99.1 ± 19.3) ng/L] (P < 0.01). Following the severity of heart failure, the level of plasma NPY increased, and the level of plasma NT decreased. The level of LVEF had negative correlative relationship with the level of plasma NPY (γ = -0.31, P < 0.05) and positive correlative relationship with the level of plasma NT (γ = 0.28, P < 0.05). The level of plasma NPY and NT in patients with heart failure had negative correlative relationship (γ = -0.26, P < 0.05). Conclusions The levels of plasma NPY, NT in patients with heart failure are unbalanced. This unbalance may participate in the damage of cardiac function.

Objective To observe the changes of plasma neuropeptide Y(NPY) and neurotensin(NT) levels in patients with essential hypertension and the relationship between those changes and damage of cardiac function. Methods Eighty patients of essential hypertension and 28 normal controls were chosen. The cardiac function was divided into 3 classes on the standard of New York Heart Association(NYHA). The plasma NPY,NT concentration was determined by radioimmunoassay. Results The level of NPY in hypertension patients was higher than that of the normal controls(P

An on-line solid phase extraction-high performance liquid chromatography ( SPE-HPLC ) system was applied in the plasma pharmacokinetic study of highly active anti-cancer compound tyrosine kinase inhibitors (TEB-415) in mouse. The on-line SPE-HPLC method associated with Ultimate3000 system which was applied to the determination of the blood drug level of TEB-415 in mouse plasma. C18 column ( Venusil MP, 150 mm × 4. 6 mm, 5μm) was used as analytical column and the mobile phase consisted of acetonitrile-5 mmol/L monopotassium phosphate buffer ( pH 3 . 5 ) at a flow rate of 1 . 0 mL/min was used as the isocratic elution. An MF Ph-1 column (10 mm×4 mm, 5 μm) was used as on-line SPE column, and water and water-acetonitrile were used as the washing solvent and elution solvent respectively. The detection wavelength was set at 262 nm. The pharmacokinetic parameters were calculated by WinNonlin 5. 2 software. The linear range of the calibration curve was between 100 and 20000 μg/L, and the limit of qualification was 20 μg/L. The extraction recovery was between 90 . 5% and 94 . 6%. The RSD of intra-day and inter-day precision was less than 3. 5%. The accuracy of short-term stability, freeze-thaw stability and long-term stability were between 91. 49% and 101. 96%. After oral medication, the mean peak time (Tmax) of TEB-415 in mice was 5. 29 h, and the mean maximum concentration ( Cmax) was 3403μg/L. The area under the curve ( AUC) of TEB-415 was 24600 μg/L·h. This drug's mean half-life was 3. 84 h, and its mean retention time (MRT) was 6. 56 h. These parameters suggested that TEB-415 had appropriate rate of absorption and elimination with preferable bioavailability.

Objective To investigate the effect of adipose tissue-derived mesenchymal stem cells (ADMSCs) transplanted by different Methods on cardiac function of rabbits with dilated cardiomyopathy.Methods 50 white ears rabbits were given doxorubicin by intraperitoneal injection to induce dilated cardiomyopathy, and randomly divided into the model group 1, model group 2 and control group. The ADMSCs isolated and cultured in vitro were transplanted into dilated cardiomyopathy rabbits of model group 1 through multi-points injection in myocardium, and were transplanted into rabbits of model group 2 through coronary artery transplant. Those in the control group were treated with IMEM medium of the same volume through multi-points injection in myocardium. Rabbits were fed for 4 week successively and then were killed to obtain heart sample, and the survival and differentiation of transplanted cells were observed through fluorescence microscope. Before transplantation and 4 weeks after transplantation, all rabbits received ultrasonic cardiogram test and haemodynamics test to determine cardiac function.Results After transplant through the two different Methods , ADMSCs could survive and differentiate in myocardial cells. 4 weeks after transplantation, the left ventricular end-diastolic volume (LVEDV) and left ventricular end-systolic volume (LVESV) reduced more significantly in the model group 2 compared with the model group 1 ( P<0.05～0.01), and LVSV, ejection fraction (EF), left ventricular systolic pressure, +dp/dtmax, -dp/dtmax increased more significantly ( P<0.05～0.01).Conclusion Transplanted ADMSCs can survive and differentiate in myocardial cells, and improve the heart function in rabbits with dilated cardiomyopathy. It is more conducive to the effectiveness of ADMSCs to improve the heart function through the method of coronary artery transplant.

Objective To evaluate the clinical value of Multi-slice Spiral CT(MSCT) and coronary artery imaging in diagnosing coronary artery myocardial bridges (MBs). Methods Image data of 285 patients from July 2005 to July 2008 who accepted both the MSCT and coronary angiography CAG at our hospital were collected. Diagnostic performance of the two Methods were analysed and the diagnostic accuracy were compared. Results In 285 cases of patients with MSCT examinations, 82 coronary artery were diagnosed in 59 cases, the detection rate of which was 20.7%. In contrast, 16 cases were diagnosed by CAG and the detection rate was 5.6%. In the 82 coronary artery diagnosed by MSCT, 42 coronary artery were single vessel, the proportion was 71.19%. Others were two vessels or three vessels. There were 67 in left anterior descending artery (LAD),in which 52 were near the middle and 15 were distal. 26 MBs diagnosed by MSCT were included in the 28 MBs diagnosed by CAG. Put CAG as the gold standard of myocardial bridge detecting, then MSCT's sensitivity was 92.86%. Conclusion MSCT coronary artery imaging can accurately show the anatomical relationship between coronary artery and myocardial and it is one of the preferred method of diagnosing coronary artery.

Objective:To observe features of ambulatory blood pressure in aged patients With hypertension complicated coro-nary heart disease (CHD)and explore the relationship betWeen abnormal ambulatory blood pressure and coronary athero-sclerosis.Methods:According to coronary angiographic results,a total of 220 aged patients With hypertension (>60 years) Were divided into hypertension+ CHD group (n=124)and single hypertension group (n=96).Both groups received 24h ambulatory blood pressure monitoring (ABPM),24h blood pressure (BP),BP variability,pulse pressure and circadian rhythm of blood pressure Were recorded.Results:Compared With single hypertension group,there Were significant increase in 24h,daytime,nighttime mean systolic blood pressure (SBP),SBP variability [dSSD (14.01±4.26)vs. (17.54± 5.51),nSSD (15.05±4.01)vs. (19.32±3.71)],pulse pressure [dPP (56.66±7.43)mmHg vs. (66.32±13.62) mmHg,nPP (55.71±6.62)mmHg vs. (63.86±7.52)mmHg] (P<0.05 all)and percentage of non-dipper rhythm (60.32% vs.82.45%)in hypertension+CHD group,P<0.01.Conclusion:There are significant increase in systolic blood pressure,SBP variability and pulse pressure,percentages of abnormal circadian rhythm in aged patients With hyper-tension complicated coronary heart disease, these abnormality may be related to occurrence and development of coronary atherosclerosis in aged patients With hypertension.

ObjectiveTo investigate the optimun approach to induce the atherosclerotic rat model.MethodsThree methods were used to induce atherosclerotic model, including: simple high fat diet feeding, high fat diet feeding with gastric perfusion of Vitamin D3, and gastric perfusion of Vitamin D3 with common rat food (splashed oil on the normal rat food with yolks and peanuts). Histopathology change of the atherosclerotic plaques in aorta was observed by normal and histopathologic sections and Hematoxylin staining.ResultsThe endarterium of aorta of the rats with simple high fat diet was smooth without atherosclerotic plaques after 2 months. 2 rats with high fat diet feeding and gastric perfusion of Vitamin D3 had formed miliary atherosclerotic plaques. In the rats with vitamin D3 and common rat food, the ring-shape calcified atherosclerotic plaques were found in the all endarterium of aorta.ConclusionGastric perfusion of Vitamin D3 once a week (3 times) combined with common rat diet could induce the atherosclerotic model successfully and decrease the mortality rate.