Objective To investigate the change and clinical significance of serum hepcidin,serum ferritin (SF),transferrin re-ceptor (sTfR)and serum iron (SI)in patients in type 2 diabetes(T2DM).Methods 130 patients with T2DM were divided into 2 groups according to the 24 hour urine microalbumin (mAlb)quantitative:group A for trace microalbumin group 45 ca-ses (mAlb30~300 mg/24 h),group B for normal albuminuria group of 85 cases,an alternate period of 45 cases of healthy physical examination for group C (control group).Results Serum hepcidin and SF of group A (42.27±32.12 ng/ml,211.6 ±107.2 ng/ml)were significantly higher than those in group B (26.12 ± 18.36 ng/ml,179.1 ± 109.7 ng/ml)and the healthy control group (P <0.05),hepcidin and SF of group B was significantly higher than that of the control group (9.47 ±1.65 ng/ml,84.41±47.10 ng/ml,P <0.01),SI and transferrin receptor(sTfR)has no statistical significance between the three groups (P >0.05).Correlation analysis showed that patients with type 2 diabetes hepcidin was positively related with SF (P <0.05),hepcidin and sTfR,SI had no significant correlation.Conclusion These results indicated that there existed serum hepcidin and SF increased iron overload and iron metabolism disorder in type 2 diabetes.Therefore,detection of serum iron and SF can be used as a predictor of diabetes early renal damage.

Objective To evaluate the childhood asthma control test(C-ACT) on the assessment of asthma by exploring the correlations among C-ACT,disease severity,the control level of pediatric asthma,disease partition and the changes in the pulmonary function as well as the score range,and to evaluate the feasibility and effectiveness of C-ACT's application to guide children's asthma control.Methods Two hundred and five children with asthma in the Pediatric Asthma Outpatient of Xiamen Hospital of Fujian University of Traditional Chinese Medicine from October 2011 to October 2015 were enrolled and randomly divided into control group and experimental group by random number table.The patients in the experimental group were monitored by the C-ACT with corresponding guidance,while patients in control group were treated without the monthly guidance of C-ACT after C-ACT involvement at the first time.C-ACT questionnaire score surveys were completed by all patients and their parents under the guidance of asthmatic specialists or nurses.Pulmonary function,disease severity and disease partition and the control level of pediatric asthma were detected and evaluated by doctors before and after treatment.The correlation between the results of C-ACT and the changes in the clinical indicators was assessed,and the improvement of lung function and the control rate of asthma were evaluated in 2 groups after 1 year.Results (1) There was no significant difference in gender,age and disease severity distribution based on the results of pulmonary function assessment between the control group and the experimental group (all P ＞ 0.05).(2) The C-ACT scores in normal,slight abnormal,moderately abnormal and severely abnormal pulmonary function were (24.79-± 2.20) scores,(21.67 ± 1.93) scores,(17.07-± 2.01) scores and (12.67 ± 1.81)scores,respectively,which showed the pulmonary function had a positive correlation with C-ACT scores (F =314.0,P ＜ 0.000 1).(3)Assessment of the reliability of C-ACT:the α value of Cronbach's coefficient for the scale was 0.867,which showed the high reliability.The correlation coefficients between the C-ACT score and the percentage of predicted value of forced expiratory volume in one second(FEV1％),the percentage of peak expiratory flow in the predicted value (PEF％) were 0.683 and 0.712,respectively,which also showed the strong reliability.(4) C-ACT scores in intermittent attacks of asthma,slight persistent asthma,moderate persistent asthma,severe persistent asthma were (24.47 ± 2.26) scores,(22.17 ± 1.86) scores,(17.42 ± 2.52) scores and (13.27 ± 2.11) scores,respectively,which showed the severity of asthma was positively associated with C-ACT scores (F =244.0,P ＜ 0.000 1).(5) C-ACT scores in controlled,partly controlled and uncontrolled asthma were (24.32 ± 2.34) scores,(18.87 ± 1.95)scores and (14.03 ± 1.32) scores,which showed the control levels of asthma had a positive association with C-ACT scores(F =394.0,P ＜ 0.000 1).(6)C-ACT scores in different disease partitions of green,yellow and red area were (24.72 ± 2.04) scores,(18.17 ± 2.03) scores and (15.06 ± 1.93) scores,which showed the diseases partition had an association with C-ACT scores (F =367.2,P ＜ 0.000 1).(7) After treatment of 3 months,6 months and 12 months,the control rates in the control group were 28.71％,67.33％,81.19％,but they were 44.23％,79.81％,95.19％ in the experimental group respectively.The control rates were higher in the experimental group than those in the control group,and the differences were significant (x2 =5.318,4.114,9.722,all P ＜ 0.05).Conclusion The C-ACT is highly correlated with pulmonary function,and our study show the C-ACT score range can help to make a quick assessment of disease severity,disease partition and the control level of children asthma.The application of C-ACT for the treatment of asthma has a good effect and it can be recommended and applied to childhood suitable for the promotion and application of children asthma clinics and community medical institutions at all levels of hospitals.

Objective:To evaluate dental and skeletal changes following slow maxillary expansion with quad helix using Cone-Beam computer tomography (CBCT).Methods:13 patients(5males and 8 females,mean age 14.38 ±2.22 years)requiring maxillary ex-pansion as a part of their comprehensive orthodontic treatment were included.Each patient had CBCT images taken pre-(T1 )and post-(T2)maxillary expansion with quad helix.Changes of the distances between bilateral canines,first premolars,second premo-lars,first molars,the width of basal bone and palatal suture were measured.paired t-test results were analyzed using SPSS 17.0 soft-ware.Results:The distances between the 4 bilateral teeth increased by (2.47 ±1.05)mm,(2.97 ±1.90)mm,(2.79 ±1.21) mm and (3.15 ±1.15)mm,the apical distances decreased by (1.19 ±0.40)mm,(2.12 ±0.68)mm,(2.02 ±0.65)mm and (1.34 ±0.63)mm,respectively.The inclination of the first molars were decreased by (4.45 ±2.86)°and (4.02 ±1.45)°on the left and right side respectively.The width of basal bone and palatal suture increased by (2.37 ±0.96)mm and (1.21 ±0.50)mm respectively,the differences between T1 and T2 were all statistically different(P <0.001).Conclusion:Quad helix expands maxil-lary arch by greater dental changes than by skeletal changes.

Adolescent ; Adult ; Age Factors ; Child ; Child, Preschool ; Electroencephalography ; Epilepsy, Temporal Lobe ; diagnosis ; etiology ; Humans ; Infant ; Male ; Middle Aged

AIM:To explore the effect of Xiangshahewei Pill(Radix Aucklandiae,Rhizoma Cyperi,Fructus Amomi,etc.)on secretion in anorectic rat model induced by Fenfluramine.METHODS:Anorectic rat model was induced by Fenfluramine in dose of 15 mg/kg for 8 days.Xiangshahewei Pill was administered orally to the rats also for 8 days.The volume of gastric juice,total gastric acid,mucus,acidity of gastric acid and activity of pepsin of rats were measured.RESULTS:Xiangshahewei Pill could improve the turbulence of gastric secretion in anorectic rat induced by Fenfluramine.Xiangshahewei Pill in dose of 0.75 g/kg(P

Objective To evaluate the efficacy and safety of therapeutic drug monitoring (TDM ) based vancomycin dose adjustment in patients with gram‐positive infections .Methods A cohort study was designed with 128 inpatients undergoing TDM in Huashan Hospital from January 2005 to September 2014 .The clinical data of these patients were used to analyze the efficacy and safety of vancomycin therapy by Cox model and survival analysis .Results The patients undergoing TDM‐based dose adjustment had a higher daily dose and blood trough concentration ,which may lead to better bacteriological efficacy and overall efficacy .Cox proportional hazards model analysis showed that TDM‐based dose adjustment is a protective factor .No safety‐related risk factor was found .Conclusions TDM‐based vancomycin dose adjustment is important for patients to achieve better outcomes in fighting gram‐positive infections .

Objective To observe the change of JAK-STAT-SOCS signal molecules after interferons alpha acting on the cancerous oral cells in 3 different degrees,namely NOK,DOK and KB cells,and to provide research foundation for the deep understanding of OSCC (oral squamous cell cancer) tumor ceils immune escape mechanism.Methods NOK,DOK,and KB cells were all cultured respectively,and then the third passage cells in the logarithmic growth phase were inoculated in cell culture plate.Blank control group of each hole was added 2 ml complete medium containing 10 ％ FPS.DMSO control group of each hole was added 2 ml complete medium containing 0.1％ DMSO.And in experimental groups containing 10 U/ml,100 U/ml,and 500 U/ml interferons,complete culture medium were added to each hole containing different concentrations of interferons alpha.CP-690550 (100 μmol/L) was added before interferons alpha was added 1 h.All were detected by RT-PCR test and Western blot test after conventional cultured for 24 h.Results RT-PCR detection showed that JAK1 and JAK2 in NOK cells had a small amount of expression,interferons alpha and CP-690550 cells could not influence the expression of JAK1 and JAK2 of NOK group,and there was no statistically significant difference (P ＞ 0.05).Interferons alpha in 100 and 500 U/ml could stimulate the increase of JAK1 and JAK2 expression in DOK and KB cells,and the differences were statistically significant (P ＜ 0.05).CP-690550 could effectively reduce the JAK1 expression of DOK and KB cells,while had no effect on the expression of JAK2,and the differences were statistically significant (P ＜ 0.05).Western blot showed that STAT1,STAT3 and pSTAT3 (Tyr705) all expressed in the control group,while pSTAT1 (Tyr701) didn't express in the control group.Interferons alpha and CP-690550 cells had no effect on STAT1,STAT3 and pSTAT3 (Tyr705) expression of NOK group,and there was no statistically significant difference (P ＞ 0.05).100 U/ml and 500 U/ml of interferons alpha could stimulate the increases of pSTAT3 (Tyr705) expression of DOK and KB cells,and the differences were statistically significant (P ＜ 0.05).While they had no effect on pSTAT1 (Tyr701) expression.CP-690550 could effectively reduce the pSTAT3 DOK and KB cells (Tyr705) expression,and the differences were statistically significant (P ＜ 0.05).Western blot showed that there were expression of SOCS1 and SOCS3 in control group.Interferons alpha and CP-690550 had no effect on SOCS1 and SOCS3 expression of NOK cell group,and there was no statistically significant difference (P ＞ 0.05).100 U/ml and 500 U/ml of interferons alpha could stimulate the increase of SOCS 1 expression of DOK and KB cells,and differences were statistically significant (P ＜ 0.05).For the expression of SOCS3,no influence.CP-690550 could effectively reduce the expression of SOCS1 of DOK and KB cells,and the differences were statistically significant (P ＜ 0.05).Conclusions Interferons alpha activate DOK JAK1 and KB cells and the expression of JAK2,mainly JAK1 activation.Interferons alpha,by activating DOK JAK1 and KB cells and the expression of JAK2,promote STAT3 phosphorylation in Tyr705 locus.Interferons alpha,by promoting STAT3 phosphorylation,further promote the expression of SOCS1,which plays the role in inhibiting interferons alpha and reducing the apoptosis.

Objective:Nuclear apoptosis-inducing factor 1(NAIF1) is a novel apoptosis gene cloned in laboratory. To analyze the binding proteins of NAIF1 by pulldown method, the fusion expression vector of truncated human nuclear apoptosis-inducing factor 1〔NAIF1(73-327)〕 was constructed, were expressed and purified the recombinant GST-NAIF1(73-327) fusion protein in E.coli.Methods:The cDNA encoding human NAIF1(73-327) was amplified by PCR and cloned into pGEX-KG vector. The GST-NAIF1(73-327) fusion protein was expressed in E.coli and purified by affinity chromatography. The purified protein was detected by SDS-PAGE, Western blot and ESI-Q-TOF-MS/MS.Results:A prokaryotic expression vector of GST-NAIF1(73-327) was constructed and the GST-NAIF1(73-327) fusion protein was expressed in E.coli at high level. SDS-PAGE analyses indicated that the purified protein was about 53 kD. Western blot and MS/MS analyses verified the recombinant fusion protein.Conclusion:An efficient method for obtaining recombinant GST-NAIF1(73-327) fusion protein had been established and it could be used for further studies on the structure and function of NAIF1.

OBJECTIVE:To investigate the acid rebound in proton pump inhibitors (PPIs) and its influential factors. METH-ODS:Totally 109 patients who treated with PPIs for 1 month in our hospital during Jan.-Dec. 2015 were collected,and telephone visit was conducted after 1 week withdrawal to the data of dyspeptic symptoms were input and scored by modified Glasgow Dyspepsia Sere-rity Score,then divided into no phenomenon group(<5 scores)and acid rebound in proton pump inhibitors group(≥5 scores). All patients were classified by basic diseases,age,gender,whether smoking and alcohol drinking,and phenomenon of acid rebound in PPIs were observed and analyzed. RESULTS:Totally 91 patients were observed,49 were classified as acid rebound in PPIs group. χ2 test showed elderly patients(χ2=5.350,P=0.021)and people with smoking and alcohol drinking(χ2=4.351,P=0.037)were associat-ed with the increased risk of acid rebound in PPIs;exclusion of the effects of gender and basic disease,Logistic regression analysis showed the risk of acid rebound in PPIs in elderly patients were 5.708 times to non-elderly patients [OR=5.708,95%CI(1.946, 16.746),P=0.002];people with smoking and alcohol drinking was 15.281 times to non-smoking and alcohol drinking [OR=15.281, 95%CI(2.748,84.965),P=0.002],the difference was statistically significant(P<0.05). CONCLUSIONS:Parts of patients show ac-id rebound after stopping PPIs,while elderly patients and patients with smoking and alcohol drinking are the high-risk population, which should be paid attention to.

Objective:To explore the mechanisms of regulation of miR-575 on the proliferation and invasion properties of non-small cell lung cancer cell (NSCLC).Methods:Real-time PCR was selected to detect the expression of miR-575 and BLID in differ ent NSCLC cell lines.CCK-8 assay was processed to measure the alternations of A549 cell proliferation at different time points after transfection of miR-575 mimic and miR-575 inhibitor.The invasion ability of A549 cells was evaluated by transwell.The targeting of BLID by miR-575 was predicted by Targetcan software and verified by dual-Luciferase assay.BLID protein expression level was detected by western blot.Results:miR-575 highly expressed in NSCLC cell lines,including A549,SPC-A1,H1299,H1650 (P＜0.001),miR-575 mimic could efficiently elevated the expression ofmiR-575 in A549 cells (P＜0.001),and strengthened the proliferation and invasion ability of NSCLC cells (P＜0.05),while,transfection of miR-575 inhibitor could down-regulate the expression ofmiR-575,and also inhibit the proliferation and invasion ability of NSCLC cells (P＜0.01).Targetscan software predicted that BLID might be the target gene of miR-575,and dual-luciferase assay revealed that miR-575 could obviously decrease the luciferase reaction of wild type BLID 3'UTR (P＜0.01),besides,miR-575 could down-regulate the protein expression ofBLID (P＜0.01).Real-time PCR results showed that NSCLC cell lines had lower level of BLID mRNA expression compared with 16HBE control cells (P＜0.001),and restore of BLID could markedly inhibited cell proliferation and invasion ability (P＜0.05),which could be reversed by miR-575 co-tranfection (P＜0.01).Conclusion:In NSCLC cells,the expression ofmiR-575 could promote cell proliferation and invasion ability by directly regulating downstream target tumor-suppressor gene BLID expression.