Objective:To separate human kidney phosphoproteome by two-dimensional gel electrophoresis.Methods: The phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography(PMAC) resin.After being concentrated and desalted,the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension.Results: The phosphorylated proteins were successfully extracted from human kidney tissues and were separated by two-dimensional gel electrophoresis.Conclusion: Phosphoprotein enrichment technique combined with two-dimensional gel electrophoresis is an effective approach to study phosphoproteome,laying a foundation for further investigation of human kidney phosphoproteins.

Objective:To develop a method of near infrared reflectance spectroscopy ( NIRS) for the rapid determination of peflox-acin mesylate for injection .Methods:The quantitative models were established by the collection of NIR spectra of pefloxacin mesylate for injection.The spectra were pretreated with the methods of vector normalization , and the spectral ranges of 9 176.2-8 169.5 cm-1 , 6 051.9-5 716.3 cm-1 and 4 509-3 999.9 cm-1 were chosen.The partial least square (PLS) was used as the regression method .Re-sults:The prediction model was established by the internal cross validation ,and the concentration range was 7.55%-77.69%.The root mean square error of cross validation (RMSECV) was 1.61%, and the correlation coefficient was 0.992 4.Conclusion: The method of near infrared reflectance spectroscopy can be used for the rapid quantitative analysis of pefloxacin mesylate for injection .

Fluorescence molecular imaging technology ( FMIT) has been developing rapidly in re?cent years. Following the development of fluorescent probes specifically targeted for gastric cancer, the signal to noise ratio has been improved in preclinical study. By combing with endoscopic technology, a new ima?ging modality called fluorescence endoscopy has been established and it is useful for the detection of gastric cancer in preclinical study. FMIT might be a promising modality in the clinical diagnosis of gastric cancer. This review summarizes the application of FMIT in preclinical studies of gastric cancer.

0.05).The cluster analysis of 20 most frequent symptoms was consistent with the frequency analysis in TCM syndrome distribution.Conclusion Age,sex has no relation with the incidence of chronic cholecystitis.TCM syndromes of stagnated heat in liver and stomach and qi stagnation in liver and stomach of chronic cholecystitis are more common.Sex,age,occupation,educational level and whether or with gallbladder stone has no relation with the distribution of TCM syndromes.

Cerenkov luminescence imaging (CLI) is a new method of optical molecular imaging,which has been successfully applied in early clinical trials.However,weak signal intensity and limited ability in tissue penetration have impeded its clinical application.Cerenkov radiation energy transfer imaging and radiation excitation fluorescence imaging were adopted to solve these problems by enabling transformation of some of the blue-weighted Cerenkov luminescence (CL) spectra to red-shifted emissions,or by exciting rare earth particles to emit visible and near infrared light.This article reviews the development of Cerenkov signal enhancement by fluorescence excitation effect.

目的比较国产重组链激酶与尿激酶静脉溶栓治疗老年人急性心肌梗死(AMI)的临床疗效。方法97例60岁以上AMI患者随机分为链激酶组(54例)和尿激酶组(43例)进行静脉溶栓治疗 ,比较两组患者的疗效及不良反应。结果冠状动脉血管开通率链激酶组75.9%,尿激酶组55.8%;并发症中,血痰:链激酶组5.6%,尿激酶组4.7%;血尿:链激酶组1.9%,尿激酶组2.3%;局部出血:链激酶组7.4%,尿激酶组4.7%;低血压:链激酶组3.7%,尿激酶组4.7%;过敏反应:链激酶组1.9%,尿激酶组2.3%;5周预后两组患者无显著性差异。结论国产链激酶静脉溶栓治疗老年人急性心肌梗死的冠状动脉血管开通率较尿激酶高,不良反应发生率与后者无显著性差异,可作为老年急性心肌梗死患者静脉溶栓治疗的首选药物。

Objective To explore the feasibility of high?resolution micro?endoscopy for diagnosis of gastric cancer. Methods A retrospective analysis of HRME pictures of gross specimen of gastric carcinoma of 20 cases of gastric cancer and normal gastric mucosa was made. Picture characteristics were studied and the HRME diagnostic criteria for normal gastric mucosa and gastric cancer tissues were developed. Based on the diagnostic criteria, a prospective study on gastric biopsy specimens of 64 cases of suspected gastric carci?noma was conducted and the diagnostic value of HRME for gastric carcinoma was evaluated by comparing HRME pre?diagnostic results with pathological findings. Results In the superficial layer of the fundic muco?sa, numerous regularly branched arranged glands as well as oval or elongated openings of the gastric pits and linear peripheral cracks were visible;the nuclei were arranged regularly. In the superficial layer of the antral mucosa, irregular or tubular openings of the gastric pits and cracked glandular cavities were visible, with the cells surrounding the gastric pits regularly arranged and the nuclei small and densely distributed. In the gas?tric carcinoma, nuclei sizes were different, arranged messy. Gland sizes were various, with unclear structure or glandular structures that could not be observed. Structure of gastric pits was damaged and normal gastric pits disappeared. After HRME imaging was performed on 64 cases of gastric biopsy specimens, the sensitivi?ty, specificity, accuracy, negative predictive value and positive predictive value of HRME diagnosis of gastric carcinoma were 96?4%( 53/55 ) , 88?9%( 8/9 ) , 95?3%( 61/64 ) , 98?1%( 53/54 ) and 80?0%( 8/10) respectively. Conclusion HRME, a new and instant pathological imaging tool with low price and simple operation, can distinguish normal gastric mucosa and gastric cancer tissue clearly, with a high diagno?sis value for gastric cancer.

Objective To find out the risk factors of early pulmonary infections after renal transplantatioa Methods The data were collected from 96 patients receiving renal transplantation between Oct. 2006 and Oct 2010, including 48 cases of early lung infection after renal transplantation as infection group, and 48 patients receiving immunosuppressive regimen at the same period as-control group. Taking the factors of lung infecition as variables, t test or chi-square test was used in univariate analyses whereas logistic regression was used in multivariate analyses. Results Single factor analysis showed that induction therapy, albumin levels, dose of steroid in 1 month after operation,family income and prophylactic SMZ treatment were related to lung infectioa Analysis of multiple variables logistic regression revealed that induction therapy, albumin levels, dose of steroid in 1 month after operation and prophylactic SMZ treatment were related to lung infection. Conclusion The correlation analysis indicated that induction therapy and dose of steroid in 1 month after operation have positive correlations with pulmonary infection, while albumin levels and prophylactic SMZ treatment have negative correlations with pulmonary infection.

Objective To fractionate phosphoproteome of mouse liver by two-dimensional (2D) liquid phase chromatography fractionation. Methods Phosphoproteins were extracted from lysates of normal mice livers by phosphate metal affinity chromatography (PMAC) resin. The phosphoproteins were exchanged by start buffer and separated by chromatofocusing in the first dimension. Then the fractions between pH 8.5 and pH 4.0 were separated by non-porous silica (NPS) reverse-phase high performance liquid chromatography (RP-HPLC). Finally, the UV maps were converted into gel-like maps by ProteoVue software. Results Phosphoproteins of mouse liver were successfully extracted and fractionated by two dimensional liquid phase chromatographic fractionation after concentration and desalt. Then pI/UV map of mouse liver phosphoproteome was successfully set-up. There are 16 fractions between pH 8.5 and pH 4.0 after chromatofocusing in the first dimension and the UV maps of each fraction were converted into pI/UV gel-like maps. Conclusions Combination of technique of phosphoproteins enrichment and 2-D liquid phase chromatographic fractionation is an effective approach to research phosphoproteome and the key base for further identification and investigation of phosphoproteins.

This article was based on the investigation of ethical research in traditional Chinese medicine (TCM) clini-cal trials among 240 ischemic stroke patients and 105 experts specialized in this field. Analysis was given on the re-search results. This article was intended to regulate the ethical review of ischemic stroke, and to protect the rights and interests of the subjects better.