Oyster protein was extracted from fresh oyster using salting-out method. It was determined by Kjeldahl procedure that the protein content was about 38. 6% and free amino-ni-trogen content of total nitrogen was about 21. 6%. Hydrolytic characteristic of oyster protein was analyzed by hydrolytic enzymes. SDS-PAGE results showed there were 4 types of protein zones. And the average molecular weight was 16172, 17633, 43227 and 57909, respectively.

0. 05), which were markedly lower in high-dose group than in low-dose group at the end of CPB and 2h after weaning from CPB (P 0. 05 ). Conclusions The high-dose aprotinin is more effective to reduce the inflammatory response to CPB than low-dose aprotinin is.

Objective To test the reliability and validity of the self-assessed questionnaire of the elderly health care needs.Methods Based on the Zuluaga-Raysmith model, the initial questionnaire was formulated by literature reviewing and interviews.Through Delphi experts consultation, a questionnaire containing 12 factors, 61 items was constructed.A total of 750 elderly residents were investigated to test the reliability and validity of the questionnaire.Results The effective rate of questionnaires was 88.67％ (665/750).The total Cronbach α was 0.871.The test-retest reliability of all the items were all greater than 0.75.The item-level content validity index were all greater than 0.80.The scale-level content validity index was 0.95.14 factors were extracted by exploratory factor analysis, explaining 66.653％ of the total variance.Conclusions The elderly health care needs self-assessed questionnaire is comprehensive with good stability, which can be used in a multi-center, large sample research.The research may help to get a deep understanding of health care needs of the elderly as well as influencing factors.

ObjectiveTo measure the perfusion parameters of liver fibrosis of dog model with 64-slice spiral CT,and compare with positive control to reflect the degree of liver fibrosis,and analyze the correlation with VEGF values.Methods Liver fibrosis was induced in dogs by intraperitoneal injection of CC14 and high fat diet.CT perfusion scan and liver biopsies were performed.The perfusion parameters were measured according to the liver fibrosis models,and the correlation with VEGF values was analyzed.ResultsThe animals in experimental group were successfully induced different degree of liver fibrosis.In normal group,the hepatic artery perfusion,portal vein perfusion and total hepatic blood flow were (28.25 ±2.19)ml/(min · 100 g),(53.53 ± 10.71)ml/(min · 100 g) and (81.78 ± 18.56) ml/(min · 100 g).The PVP and TLP values of the liver fibrosis models of 0 - 4 stages declined gradually,and it had statistical significance,while the HAP values increased.The PVP and TLP values were positive correlated with fibrosis stage.The VEGF values of the pathological models of 0 - 4 stages ascended significantly.Conclusions The dog liver fibrosis models that are similar to human hepatic fibrosis have been successfully induced by low dose CC14 and high fat diet.CT perfusion can be used to monitor the tendency of the hemodynamic in different degree of fibrosis.VEGF may play an important role during liver fibrogenesis.

Objective To investigate the clinical characteristics,treatment effect,long-term follow up results,guanosine triphosphate (GTP) cyrclohydrolase Ⅰ (GCH Ⅰ)gene and tyrosine hydroxylase(TH) gene mutations in patients with dopa-responsive dystonia (DRD).Methods The clinical features of 3 families with 4 affected members were analyzed and all of 4 patients were screened for mutations of the GCH Ⅰ gene and TH gene with DNA sequences.Results Four patients were females,average age at onset was (15.3 ± 5.6) years (range:from 9 to 20 years).The initial symptoms were a gait disorder,stiffness or tremor of the lower limbs in all patients presented with diurnal fluctuation.As the increase of disease duration,bilateral hand tremor was found in three patients,systemic torsion was found in one patient and torticollis was found in one patient.All patients' symptoms were in complete remission after administration of low dose of levodopa.Four patients were followed up for 0.5 to 10.0 years,and all were still responsive to the levodopa treatment and effective dosage was decreased as the increase of the disease duration.No longterm side effects of levodopa had occurred after long-term treatment.One patient was found to have c.607G ＞A(p,Gly203Arg) heterogenetic mutation in GCH I gene.Molecular analysis revealed a compound heterozygous mutation in the TH gene (p.Y447Ter and p.V468M) in one patient.No point mutations in both genes were found in other patients.Conclusions DRD patients have dramatic and sustained response to levodopa and no long-term side effects of levodopa after long-term treatment.The detection of GCH Ⅰ and TH gene mutations is helpful in early diagnosis but the negative results could not exclude the diagnosis of DRD.

Objective: To identify Persicae Semen and its sibling species, and to secure the quality and clinical safety of this medicinal material. Methods: The internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA of Persicae Semen and its sibling species were amplified and bidirectionally sequenced by DNA barcoding. Sequence assembly and consensus sequence generation were performed by the CodonCode Aligner 4.1. The genetic distances were computed by MEGA 5.0 in accordance with the Kimura 2-parameter (K2P) model, and the phylogenetic tree constructed by the neighbor-joining (NJ) method. Results: The length of ITS2 sequences of the two origin plants of Persicae Semen was between 212 bp to 213 bp. Their intraspecific genetic distance was much lower than the interspecific genetic distance with their sibling species. The ITS2 sequence possessed the character of good stability and low intra-specific sequence variation. In the NJ tree, both Prunus persica and P. davidiana were clustered into one large branch, and clearly separated with their sibling species. Conclusion: ITS2 can be used to effectively distinguish Persicae Semen from its sibling species, which can provide a reference for the iden-tification of other Chinese medicine and its sibling species.

Objective To investigate the effect of the TinniTest tinnitus comprehensive diagnostic and therapeutic apparatus for treating tinnitus and to analyze its clinical application value .Methods The TinniTest tinnitus comprehensive diagnostic and thera-peutic apparatus was adopted to inquiring ,evaluating ,diagnosing ,testing on 100 tinnitus patients .On this basis the tinnitus shelter treatment and the psychology consultation treatment were performed .Results The tinnitus disability quantitative test table was used to evaluate 100 patients .The evaluation results showed 12 cases of level 1 ,34 cases of level 2 ,28 cases of level 3 ,18 cases of level 4 and 4 cases of level 5 .The evaluation results of these 100 cases were classified to the mild degree≤ level 2 and the moderate degree >level 3 .Then the tinnitus test was performed .Among the cases of mild degree tinnitus ,4 cases were completely cured ,26 cases were significantly effective ,14 cases were effective and 2 cases were invalid ,the effective rate was 95 .6% .However among the cases of severe degree tinnitus ,0 case was completely cured ,28 cases were significantly effective ,18 cases were effective and 8 cases were invalid ,the effective rate was 85 .2% and the total effective rate was 90 .0% ,the difference in the effects between the two groups had no statistical significance(χ2 =1 .99 ,P>0 .05) .No adverse reactions occurred in all 100 cases .Conclusion The TinniT-est tinnitus comprehensive diagnostic and therapeutic apparatus can provide the comprehensive and accurate evaluation and the sci-entific tests to the tinnitus patients ,which has the definite effect for treating subjective tinnitus and the same effects for both mild and sever tinnitus patients .

Objective To investigate the imaging characteristics of rabbit liver VX2 tumor with contrast-enhanced ultrasonography(CEUS) and multidetector spiral CT(MDCT), to explore the criterion of phase timing apllicable to the model.Methods CEUS and MDCT were acquired in thirty-three New Zealand rabbits with hepatic VX2 tumors.The initial time of hepatic arterial phase was verified as the time the hepatic artery beginning to enhance,the initial time of portal phase as the time the VX2 tumor reaching peak enhancement and the initial time of hepatic parenchymal phase as the time the hepatic parenchyma reaching peak enhancement.The enhancing characteristics were observed.The time-intensity curve of VX2 tumor and peripheral parenchyma in CEUS was analysed and compared with the time-desity curve of abdominal aorta, portal vein, hepatic parenchyma and tumor in MDCT.Results The initial time of heptic arterial phase,portal phase and parenchymal phase in CEUS and MDCT was (6.82±1.36)s and (9.43±2.23)s,(11.64±2.03)s and (13.77±2.01)s,(20.24±4.17)s and (22.71± 4.58)s,respectively.The initial time of each phase in CEUS was earlier than that in MDCT (P<0.01).The contrast enhancing characteristics in CEUS and MDCT were consistent as fast wash-in and fast wash-out.Conclusions The status of hemodynamic perfusion in rabbit hepatic VX2 tumor could be well displayed by CEUS and the phase timing criterion concluded in this experimental study could offer fundmental theory basis for relevant imaging evaluation.

A cross-sectional study in 116 patients with polycystic ovary syndrome (PCOS) was carried out by using the short-form-36 health survey (SF-36), serf-rating anxiety scale ( SAS), and self-rating depression scale (SDS) from January 2008 to April 2008 in our reproduction center. In comparison with the healthy controls, the PCOS patients showed decreased quality of life (QOL) for all items of SF-36 (P<0.05) except for body pain or role-physical scales. The differences in SAS and SDS between the PCOS and the healthy controls were statistically significant (P<0.05). Our study results confirm the negative impacts of PCOS over the quality of life;subfertility, obesity, hirsutism, acne, complications, menstrual irregularity and educational levels might contribute to the reduced QOL.