Objective To analyze the application effect on gynecological laparoscopic surgery patients by different dexmedetomidine doses.Methods One hundred gynecological laparoscopic surgery patients were divided into control group and observation group by random digits table method with 50 cases each.The patients in observation group were given dexmedetomidine 0.5 μ g/kg intravenous injection 15 min before induction of anesthesia intubate,the patients in control group were given dexmedetomidine 1.0 μ g/kg,then 0.4 μ g/(kg· min) continuing venous pumping.The induction of anesthesia program in the 2 groups was midazolam,fentanyl citrate,propofol,cisatracurium besilate intravenous injection,maintaining intraoperative depth of anesthesia program was remifentanil and propofol by continuing venous pumping,and the maintaining muscle relaxation program was cisatracurium besilate interruptable injection.The patients in the 2 groups were given dezocine 0.1 mg/kg intravenous injection 30 min before the end of operation.The man-machine system engineering (MMSE) score,restlessness degree (using restlessness score),sedation effect (using Ramsay sedation score),analepsia time,extubation time and drug adverse reaction were compared.Results The rates of postoperative cognitive dysfunction and drug adverse reaction in observation group were significantly lower than those in control group [10％(5/50) vs.32％(16/50),10％(5/50) vs.40％(20/50)],Ramsay sedation score,analepsia time and extubation time were significantly lower than those in control group [(1.04 ± 0.59) scores vs.(4.84 ± 0.63) scores,(20.14 ± 4.32) min vs.(26.57 ± 4.28) min,(20.17 ± 4.23) min vs.(27.26 ± 4.34) min],there were statistical differences (P ＜ 0.05).There was no statistical difference in MMSE score before anesthesia and 24 h after anesthesia between the 2 groups (P ＞ 0.05),but MMSE score 1,6,12 h after anesthesia in observation group was significantly higher than that in control group [(23.93 ±3.51) scores vs.(20.34 ±3.45) scores,(25.87 ±4.46)scores vs.(21.34 ±4.63) scores,(27.53 ± 4.22) scores vs.(23.49 ± 4.31) scores],there was statistical difference (P ＜ 0.05).Conclusions Dexmedetomidine in accordance with 0.5 μ g/kg can reduce the incidence of postoperative drug cognitive dysfunction in the patients after gynecological laparoscopic surgery,it has good calming effect and less adverse reactions.It is worthy of popularization and application in the clinical work.

Helicobacter pylori (H.pylori) infection rate is increasing these years.The incidence of related diseases subsequently rose.Hp infection can not only cause digestive diseases,but also lead to idiopathic thrombocytopenic purpura (ITP),iron deficiency anemia,and indigestion etc.which can affect the growth and nutritional health of children.H.pylori has become a global issue,both international and domestic colleagues have reached a consensus on treatment for adults.According to the new conscnsus,the aim of this article is to study the diagnosis and treatment options for children with Hp infection.

Objective To explore a new method in treating acute closed rupture of the Achilles tendon. Methods 22 cases of closed rupture of the Achilles tendon, which had been wounded within one week, were treated with short incision and tenocutaneous relaxing suture from 1994 to August 2001. Results The duration of follow up ranged from eight months to five years. According to Arner Lindholm criteria of therapeutic evaluation, excellent results were obtained in 19 (86.4% ), good in 3 (13.6% ), with the excellent and good rate being 100% . Conclusion Short incision and tenocutaneous relaxing suture is an effective treatment for acute closed rupture of the Achilles tendon because of its less suture tension and more blood supply protection. [

Objective To illustrate the different levels of fundamental nursing skills of undergradu ate nursing students,and explore the characteristics of the assessment method.Methods With descriptive statistics,one-way analysis of variance after rank transformation,and multiple comparison (Scheffe),we made an analysis of 1 340 assessment records of fundamental nursing skills.Results The score distributions of all the skills,but the skill of measuring vital signs,conformed to Gaussian distribution.The overall distribution shaped in peak state and was negative skewness,showing bias towards 70 to 90 score levels.The score levels of 60 and 70 were significantly higher than that of their surrounding levels.The scores of aspiration of sputum,oxygenic therapy and aseptic technique were the lowest,while the scores of closed bed making and intramuscular injection were the highest.Conclusions The score distribution of fundamental nursing skills is rational,which shows the methods of teaching and assessment are generally believable.But the individual bias cannot be ignored.The majority of students get high grades.Different levels do exist among different skills because of abstract or concrete characteristics and principal matters.

An experimental study was carried out on the isolated bone cells, from the embryonic calvarium of Sprague-Dawley rats which were taken out in vitro culture in the later period of gestation. Observation of the early stage of culture was carried out with phase contrast microscope, H. E. staining and AcP and AlP technique. It was found that both the osteogenic cells of the cambium layer of the periosteum and the undifferentiated cells from the bone marrow could develop into large squamous-shaped cells. These cells possessed many processes connected with the adjacent cells and stained light red color with Sirius Red but revealed no birefringency under polarized microscope. This indicates that these cells are precursors of collagen formation. The negative reaction with the alkaline phosphatase staining pointed out that these cells still belong to the osteogenic cells.

Objective To observe the characteristics of obesity in rats with metabolic syndrome (MS) and to study the role of ERK1/2 and p38MAPK in the production of adipose tissues. Methods The metabolic parameters, weight of adipose tissue, waist circumference (WC), Lee's index, distribution area of adipocytes and the number of big adipocytes in the rats were measured. Adipose tissues and liver were histopathologically observed. ERK1/2 and p38MAPK of adipose tissue were determined using Western blot. Results (1) The blood pressure, fasting insulin level, LDL-C, TG and FFA in rats with MS were markedly higher than that in control rats (P

Objective To evaluate the reliability and validity of Short-form Arthritis Impact Measurement Scales 2(AIMS2-SF).Method AIMS2-SF not including the role component was used to evaluate Quality of Life(QOL) of 51 arthritis patients,then its test-retest reliability and the internal consistency reliability were tested,and factor analysis was applied to determine its construct validity.Results The Intra-class correlation coefficients(ICCs) of test-retest reliability of AIMS2-SF ranged from 0.60 to 0.80,and the Cronbach's ? coefficient for internal consistency reliability ranged from 0.65 to 0.83.Furthermore,the factor analysis confirmed the construct validity for AIMS2-SF.Conclusions The study suggested that the AIMS2-SF had satisfactory reliability and validity,then it is applicable for the evaluation of QOLs of Chinese patients with arthritis.

[Objective] To examine the specific gene and protein expression of primary and passaged chondrocytes in normal oxygen tension and hypoxia.[Methods]Articular chondrocytes were isolated from limb joint cartilage of C57BL/6 mice(3~5 days).Primary chondrocytes(P0)and passaled chondrocytes(P1,P2)were cultured in normal oxygen tension and hypoxia respectively for two days.The mRNA levels of collagen II,aggrecan,sox9,Indian hedgehog(ihh),parathyroid hormone-related protein(PTHrP),bone morphogenetic protein(BMP)-4 and wnt5a were examined with RT-PCR,and protein levels of collagen II,aggrecan were examined with immunohistochemistry and toluidine blue staining.[Results]During the culture of primary chondrocyte,the expression of collagen II and aggrecan increased under hypoxia,mRNA levels of collagen II,wnt5a increased and ihh decreased at the same time.The mRNA levels of special genes and differentiation related genes of passaged chondrocytes were not altered under hypoxia.[Conclusion]Protein and mRNA level of Collagen II of primary chondrocyte under hypoxia increased.It may be regulated by chondrocyte differentiation gene ihh,and wnt5a.The chondrocyte phenotype could not be resumed under hypoxia through short-term monolayer culture in vitro.

Objective To evaluate the association of prognostic factors and the interval between surgery and radiotherapy in postoperative radiotherapy for non small cell lung cancer (NSCLC). Methods Between December 1982 and December 1992, 132 patients with NSCLC were retrospectively analyzed. The time interval between surgery and radiotherapy ranged from 12 to 90 days, with a median of 36 days. All patients received D T 40～72 Gy. Results Cox proportional analysis showed that stage, Karnofsky performance status, and the interval between treatments were important prognostic factors. Patients with a long interval of 37～90 days had a better survival than those with a short interval of 12～36 days (P