Objective To observe the effects of anti lipopolysaccharide (LPS) immunoglobulin Y (IgY) on LPS in vitro . Methods The direct in vitro neutralization of different concentrations of IgY and LPS was conducted for the detection of the remained amount of LPS to observe the dose effect relationship. The primary cultured human umbilical vein endothelial cells (HUVECs) were cultured for 48-72 h after addition of certain concentrations of fluorescein isothiocyanate LPS (FITC LPS) and IgY. Changes of the fluorescent intensity in HUVECs were observed at different time points. After addition of certain concentrations of LPS and IgY for incubation with HUVECs for 4, 8, and 12 h, the changes of lactate dehydrogenase (LDH) activity were measured. Results The dose effect relationship was satisfactory when the ratio was 16︰1. At 4, 8, and 12 h, fluorescent intensity was somewhat strong in FITC LPS group, but weak in IgY group, especially at 8 and 12 h. The LDH activity in medium increased obviously after incubation for 8 h in LPS group ( P

Objective To investigate ?-cell early insulin secretion function and insulin resistance in different durations and grades patients with hypertension.Methods One hundred and six middle and old age(58.4?8.5) years hypertension patients were enrolled.According to hypertension durations,patients were categorized as ≤1 year group(n=35),1.1-4.9 years group(n=34),≥5 years group(n=37);while based on grades of hypertension,patients were divided as 1st grade(140-159/90-99 mmHg,n=53),2nd grade(160-179/100-109 mmHg,n=30),3rd grade(≥180/≥110 mmHg,n=23).Plasm glucose,HOMA-IR,?I30/?G30 were determined and compared among groups.Results Patients with longer duration(≥5 years) had most seriously impairment of ?-cell early secretion(?I30/?G30)[≥5 years(2.1?4.5) vs 1.1-4.9 years(10.9?4.2),vs ≤1 year(10.3?4.2),all P

At present, due to the opening and circulation of salt market, potassium iodate iodized salt, potassium iodide iodized salt, seaweed iodized salt and non-iodized salt may exist simultaneously in the edible salt market of a region, which brings new problems to the work of testing iodine content of edible salt in the laboratory. In order to carry out salt iodine detection for prevention and monitoring of iodine deficiency disorders, this paper introduces some methods which established by the author for a rapid qualitative, semi-quantitative and quantitative detection of iodized salt of different forms of iodine and non-iodized salt. And further application and discussion of the methods are described for reference of iodized salt monitoring.

Objective To explore the conversion rate of iodate ions (IO3-) being reduced to iodide ions (I-) by ascorbic acid (C6HsO6,VC) in simulated human gastric juice,and to provide references for further safety evaluation of edible salt iodized with potassium iodate.Methods An ion chromatography method was developed to detect iodide ions in simulated gastric juice.The conversion rate of iodate ions being reduced to iodide ions was used as the index for scavenging rate of iodate ions.In simulated gastric juice in vitro and in 37 C water bath,the scavenging effects of VC on iodate ions were determined in simulated gastric juice with different VC concentrations and simulated gastric juice acidities,as well as for different reaction time.Ion chromatography column:Dionex IonPac AS19 (250 mm × 4.0 mm);eluent:KOH 30 mmol/L (online produced),isocratic eluting,flow rate 1.0 ml/min,injection volume 100 μl,and detected by a conductivity detector.Results Performance of the method:within the range of 0-5 000 μg/L,iodide ions concentration and the chromatographic peak area had a good linearity (correlation coefficient r =0.999 7),and the detection limit of iodide ions was 20 μg/L.For quantification of iodide ions in simulated gastric juice,the relative standard deviation (RSD) of repeated measure for 6 times was ＜ 2.0％,the standard addition recovery rate was 97.6％-102.4％,and the overall average recovery rate was 99.4％.In the simulated gastric juice with a pH of 1.4 containing 5 mg/L and ≥ 10 mg/L VC,the reaction time to achieve 100％ conversion rate of iodate ions being reduced to iodide ions was 5 min and 2 min,respectively.In the simulated gastric juice with a pH of 3 containing 10 mg/L ascorbic acid,the reaction time to achieve 100％ conversion rate was 15 min.VC quantitatively reduced iodate ions to iodide ions by the stoichiometric relationship between reactants of the reduction reaction equation,and every 100 μg VC quantitatively reduced 24.0μg of iodine in iodate to iodide ions.Conclusions In simulated gastric juice,the reaction of iodate ions being reduced to iodide ions by VC is a stoichiometric reaction with relatively fast reaction rate,the scavenging rate of iodate ions by VC within the concentration level in human gastric juice can reach 100％.The results prompt that the iodate ions from edible salt iodized with potassium iodate in daily diet are reduced to iodide ions mainly in the human stomach.

Objective To study the psychological stress level of medical nurses when the hospitalized cancer patients died and to investigate the related factors.Methods Toally 72 medical nurses on duty who encountered cancer patient's death between May 2013 to November 2015 were enrolled in the study.SCL-90 was used to investigate the mental stress levels in the medical nurses on days 3 and 7 after the patient's death,which were compared with the norm.The correlations between psychological stress level and related factors were studied.Results The score by SCL-90 on day 3 was significantly higher than that on day 7 (147.15±29.09 vs.133.62±20.37,t=6.64,P=0.00).Compared to the norm,the scores on 9 dimensions of SCL-90 were significantly different 3 days after the patient's death;dimensions of SCL-90 were significantly different 7 days after parternt's death (P＜0.05).Professional working was factor for medical nurse's acute stress disorder.Conclusion Cancer patients' deaths render huge impact on medical nurses,with acute stress disorder most manifest.In order to improve the the psychological quality of nurses,especially the young and inexperienced ones,we have to enhance the death education,helping them to seek for social support.

Objective To evaluate the reliability and validity of Short-form Arthritis Impact Measurement Scales 2(AIMS2-SF).Method AIMS2-SF not including the role component was used to evaluate Quality of Life(QOL) of 51 arthritis patients,then its test-retest reliability and the internal consistency reliability were tested,and factor analysis was applied to determine its construct validity.Results The Intra-class correlation coefficients(ICCs) of test-retest reliability of AIMS2-SF ranged from 0.60 to 0.80,and the Cronbach's ? coefficient for internal consistency reliability ranged from 0.65 to 0.83.Furthermore,the factor analysis confirmed the construct validity for AIMS2-SF.Conclusions The study suggested that the AIMS2-SF had satisfactory reliability and validity,then it is applicable for the evaluation of QOLs of Chinese patients with arthritis.

On the basis of a survey of the health manpower in the city, the authors probe into the issues of the allocation and training of the health manpower at the township and village levels. They put forward the view that it is imperative to establish and amplify the mechanism for the management of township and village health personnel and to rationally allocate and effectively utilize human resources in the health sector; to perfect the system of on the job training and assessment of health personnel and improve the quality of the working staff; and to integrate the management and training of the health manpower with other health tasks and strive for overall planning, unified operation and coordination.

Objective To probe into the proper ratio of doctors and nurses and appropriate nursing programs in community health service. Methods A number of methods were used, including task analysis, use of old handsexperience, sociological survey, and literature review. Results Medical consultation took 1 105 minutes, taking blood pressure took 141.5 minutes, transfusion took 493.5 minutes, intramuscular injection took 44.5 minutes, and drawing blood took 77.5 minutes. According to the above results, the ratio of doctors and nurses was respectively 3.18∶1, 0.94∶1, and 2.02∶1. Also, by analyzing in a comparative way the nursing programs already developed both at home and abroad and taking into consideration the reality, suggestions were put forward as to the kind of nursing program communities ought to start. Conclusion The findings are of referential value to administrative departments of health and medical institutions.

ObjectiveTo investigate the possible relationship between choroidal neovascularization (CNV) and macular choroidal watershed zones (CWZ) in patients with age-related macular degeneration (AMD). MethodsFifty-seven selected indocyanine green video angiograms (ICGA) of 57 patients (57 eyes) with AMD were evaluated, and 35 ones of the healthy fellow eyes of 35 patients with unilateral non-AMD fundus diseases were selected as age-matched control. The video angiograms were evaluated for investigating the relationship between CNV and macular CWZ. ResultsIn 57 eyes with AMD, 35 (61.4%) had macular CWZ, while in 35 control patients only 3 (8.57% ) had. The difference was significant (P