1.Discussion on the detection methods of iodized salt with different forms of iodine and non-iodized salt
Chinese Journal of Endemiology 2021;40(2):146-149
At present, due to the opening and circulation of salt market, potassium iodate iodized salt, potassium iodide iodized salt, seaweed iodized salt and non-iodized salt may exist simultaneously in the edible salt market of a region, which brings new problems to the work of testing iodine content of edible salt in the laboratory. In order to carry out salt iodine detection for prevention and monitoring of iodine deficiency disorders, this paper introduces some methods which established by the author for a rapid qualitative, semi-quantitative and quantitative detection of iodized salt of different forms of iodine and non-iodized salt. And further application and discussion of the methods are described for reference of iodized salt monitoring.
2.?-cell Early Insulin Secretion Function and Insulin Resistance in Various Durations and Grades in Patients with Hypertension
Chinese Journal of Hypertension 2007;0(07):-
Objective To investigate ?-cell early insulin secretion function and insulin resistance in different durations and grades patients with hypertension.Methods One hundred and six middle and old age(58.4?8.5) years hypertension patients were enrolled.According to hypertension durations,patients were categorized as ≤1 year group(n=35),1.1-4.9 years group(n=34),≥5 years group(n=37);while based on grades of hypertension,patients were divided as 1st grade(140-159/90-99 mmHg,n=53),2nd grade(160-179/100-109 mmHg,n=30),3rd grade(≥180/≥110 mmHg,n=23).Plasm glucose,HOMA-IR,?I30/?G30 were determined and compared among groups.Results Patients with longer duration(≥5 years) had most seriously impairment of ?-cell early secretion(?I30/?G30)[≥5 years(2.1?4.5) vs 1.1-4.9 years(10.9?4.2),vs ≤1 year(10.3?4.2),all P
3.Effects of anti-lipopolysaccharide egg yolk immunoglobulin on LPS in vitro
Qiang LI ; Yaping ZHANG ;
Journal of Third Military Medical University 2003;0(07):-
Objective To observe the effects of anti lipopolysaccharide (LPS) immunoglobulin Y (IgY) on LPS in vitro . Methods The direct in vitro neutralization of different concentrations of IgY and LPS was conducted for the detection of the remained amount of LPS to observe the dose effect relationship. The primary cultured human umbilical vein endothelial cells (HUVECs) were cultured for 48-72 h after addition of certain concentrations of fluorescein isothiocyanate LPS (FITC LPS) and IgY. Changes of the fluorescent intensity in HUVECs were observed at different time points. After addition of certain concentrations of LPS and IgY for incubation with HUVECs for 4, 8, and 12 h, the changes of lactate dehydrogenase (LDH) activity were measured. Results The dose effect relationship was satisfactory when the ratio was 16︰1. At 4, 8, and 12 h, fluorescent intensity was somewhat strong in FITC LPS group, but weak in IgY group, especially at 8 and 12 h. The LDH activity in medium increased obviously after incubation for 8 h in LPS group ( P
4.An experimental study on the scavenging effect of ascorbic acid on iodate ions in simulated gastric juice
Yaping ZHANG ; Shuqiong ZHANG ; Na LI
Chinese Journal of Endemiology 2017;36(3):176-181
Objective To explore the conversion rate of iodate ions (IO3-) being reduced to iodide ions (I-) by ascorbic acid (C6HsO6,VC) in simulated human gastric juice,and to provide references for further safety evaluation of edible salt iodized with potassium iodate.Methods An ion chromatography method was developed to detect iodide ions in simulated gastric juice.The conversion rate of iodate ions being reduced to iodide ions was used as the index for scavenging rate of iodate ions.In simulated gastric juice in vitro and in 37 C water bath,the scavenging effects of VC on iodate ions were determined in simulated gastric juice with different VC concentrations and simulated gastric juice acidities,as well as for different reaction time.Ion chromatography column:Dionex IonPac AS19 (250 mm × 4.0 mm);eluent:KOH 30 mmol/L (online produced),isocratic eluting,flow rate 1.0 ml/min,injection volume 100 μl,and detected by a conductivity detector.Results Performance of the method:within the range of 0-5 000 μg/L,iodide ions concentration and the chromatographic peak area had a good linearity (correlation coefficient r =0.999 7),and the detection limit of iodide ions was 20 μg/L.For quantification of iodide ions in simulated gastric juice,the relative standard deviation (RSD) of repeated measure for 6 times was < 2.0%,the standard addition recovery rate was 97.6%-102.4%,and the overall average recovery rate was 99.4%.In the simulated gastric juice with a pH of 1.4 containing 5 mg/L and ≥ 10 mg/L VC,the reaction time to achieve 100% conversion rate of iodate ions being reduced to iodide ions was 5 min and 2 min,respectively.In the simulated gastric juice with a pH of 3 containing 10 mg/L ascorbic acid,the reaction time to achieve 100% conversion rate was 15 min.VC quantitatively reduced iodate ions to iodide ions by the stoichiometric relationship between reactants of the reduction reaction equation,and every 100 μg VC quantitatively reduced 24.0μg of iodine in iodate to iodide ions.Conclusions In simulated gastric juice,the reaction of iodate ions being reduced to iodide ions by VC is a stoichiometric reaction with relatively fast reaction rate,the scavenging rate of iodate ions by VC within the concentration level in human gastric juice can reach 100%.The results prompt that the iodate ions from edible salt iodized with potassium iodate in daily diet are reduced to iodide ions mainly in the human stomach.
6.C-fos gene expression in local cerebral tissues of canines following craniocerebral gunshot wounds
Yaping FENG ; Xiang ZHANG ; Xiusong BAI
Chinese Journal of Trauma 2003;0(07):-
Objective To study the gene expression and the expression change rule of c-fos in the canines posterior to craniocerebral gunshot wounds in order to provide the experimental base for further understanding of the mechanism of gunshot craniocerebral injury. Methods Using the canine model of penetrating craniocerebral injury made by the bullets of the small-calibre rifle made in Germany, the c-fos expressions in the control cerebral tissues and the contusion and concussion areas of the trajectory and the brain stem neurons at different time points after trauma were observed by immunohistochemical method. Results Feeble c-fos expression was detected in the control group. After craniocerebral gunshot wounds, the c-fos expression increased in the contusion and concussion areas of the trajectory and in the brain stem at the 30th minutes, reached the peak at the 2nd hour and began to decrease at the 3rd hour. The c-fos expression in the concussion area was much more significant than that in the contusion area ( P
7.Reflection on the allocation and training of the health manpower at the township and village levels in our city
Yaping ZHANG ; Linli GONG ; Liuquan HOU
Chinese Journal of Hospital Administration 1996;0(12):-
On the basis of a survey of the health manpower in the city, the authors probe into the issues of the allocation and training of the health manpower at the township and village levels. They put forward the view that it is imperative to establish and amplify the mechanism for the management of township and village health personnel and to rationally allocate and effectively utilize human resources in the health sector; to perfect the system of on the job training and assessment of health personnel and improve the quality of the working staff; and to integrate the management and training of the health manpower with other health tasks and strive for overall planning, unified operation and coordination.
8.Myocardial protection of L-carnitine in patients with acute myocardial infarction undergoing primary percutaneous coronary intervention
Yaping LI ; Lijun GUO ; Fuchun ZHANG
Chinese Journal of Interventional Cardiology 1996;0(04):-
50% resolution of elevated ST-segment in 1h after PCI was increased in the L-CN treated group. LVEDD was less but LVEF was higher in the treatment group compared with the control group 3 months after PCI. Conclusion L-CN has significant myocardial protection effects in patients with AMI undergoing primary PCI, which is characterized by inhibition of the reperfusion injury , reduction of infarction area and improvement of left ventricular remodeling.
9.Techniques of performances improvement of LED light source for instruments
Zhenfang LIU ; Yaping HU ; Wenchao ZHANG
Chinese Medical Equipment Journal 1989;0(04):-
This paper introduces the principle and characteristics of the LED constant-current and constant-power drive circuit for instrument's excitation light source.It also analyzes the steady-state and dynamic performances of the drive circuits.At last,it discusses the improving measures of the precision and stability for the drive circuit of LED excitation light source.
10.Relationship between choroidal neovascularization and macular choroidal watershed zones in patients with age-related macular degeneration
Yaping LI ; Xiaoxin LI ; Xiaoguang ZHANG
Chinese Journal of Ocular Fundus Diseases 1999;0(02):-
ObjectiveTo investigate the possible relationship between choroidal neovascularization (CNV) and macular choroidal watershed zones (CWZ) in patients with age-related macular degeneration (AMD). MethodsFifty-seven selected indocyanine green video angiograms (ICGA) of 57 patients (57 eyes) with AMD were evaluated, and 35 ones of the healthy fellow eyes of 35 patients with unilateral non-AMD fundus diseases were selected as age-matched control. The video angiograms were evaluated for investigating the relationship between CNV and macular CWZ. ResultsIn 57 eyes with AMD, 35 (61.4%) had macular CWZ, while in 35 control patients only 3 (8.57% ) had. The difference was significant (P