s:Case teaching is one of the vital methods in improving practical medical students.With the development of heuristic teaching,case teaching is becoming increasingly crucial in daily teaching.Through 3 year-case-teaching practice in medical physics teaching,case teaching has been bettered and systemized.Case and case teaching has become one of the standard teaching methods in teacher education.For the application of case teaching to medical physics teaching,correct understanding of the definition is needed for the selected cases.The diversity of teaching is also imperative for the feature of medical physics teaching.Based on experiences,it reinforces students' perception,creative ability and cultivates their practical ability for the job prospect.Meanwhile,it enhances instructors' teaching level by win-win effect.

The construction ofteachingteamin institutes is an effective wayfor organizingthe basic teaching constitution and improving teaching quality.We obtained the excellent teaching team of Chongqing in 2008.Our practice and thinking will be introduced in this paper,so as to provide the reference for teaching team construction in institutes.

Objective To investgate the signal transduction and regulation in erythropoiesis by total saponins of Panax ginseng(TSPG) to clarify the mechanism for Panax Ginseng promoting hematopoiesis.Methods The colony-forming ability of mononuclear cells(MNCs) from human umbilical cord blood was assayed in methylcellulose assay systems,various dilutions of TSPG(0,10-100mg/L) were then added to each methylcellulose culture mixture;MNC proliferation in response to erythropoietin(Epo) was measured by MTT;To investigate whether the preferential differentiation of MNCs into erythroid lineage cells was caused by an increase in the expression of erythropoietin receptor(EpoR) on the cells,immunoblotting analyses were performed;Immunoprecipitation of JAK_2/STAT_5 was used for the effect of TSPG on Epo/EpoR-induced tyrosine phosphorylation of JAK_2/STAT_5. Results 1.TSPG(10-75mg/L) can promote the colony formation of BFU-E,CFU-E,and the preferential differentiation into erythroid lineage cells was induced from 25mg/L of TSPG;2.Using the colorimetric MTT assay,MNCs exhibited proliferous responses to Epo(2?10~3-5?10~4IU/L) reaching maximum at 5?10~3IU/L Epo;3.The addition of TSPG did not increase the expression of EpoR;4.After MNCs were incubated in the presence of with or without TSPG for 24 h,the cells were stimulated with Epo for 0,2,5,30min,and tyrosine phosphorylation JAK_2/STAT_5 was measured.The pretreatment with TSPG enhanced Epo-induced tyrosine phosphorylation of JAK_2 and STAT_5(STAT_(5a) and STAT_(5b)).Conclusion In the present study,we have shown that the Epo/EpoR-mediated signals are enhanced,and JAK_2/STAT_5 signals plays an essential role in the effect of TSPG on erythropoiesis,whereas the expression level of EpoR remains unchanged.

Objective Panax ginseng is a well-known Chinese traditional medicine functional in “invigorating qi”,and saponins are one of its main effective fractions.Our study investigates the effect of total saponins of panax ginseng(TSPG)on human hematopoiesis at early stage and its possible regulative mechanism to clarify the hematonic mechanism of Panax ginseng. Methods The techniques of culturing hematopoietic progenitor cells in vitro,bioassay of hematopoietic growth factor(HGF),immunocytochemistry and nucleic acid in situ hybridization were used to study the effect of TSPG on IL-3’s expression in hematopoietic stromal cell and its possible mechanism. Results TSPG directly added into culture system in vitro can markedly increase the colony forming yields of CFU-Mix;the different conditioned culture media prepared with TSPG can promote the proliferation and differentiation of CFU-Mix;the protein and mRNA expression of IL-3 in BMSC and cells of EcV304,THP induced by TSPG has been much intensified.Conclusion TSPG may regulate hematopoiesis at early stage by activating IL-3's gene expression in hematopoietic stromal cell,which is the most important element in hematopietic inductive microenvironment(HIM).

BACKGROUND: Disturbance of lipid metabolism is responsible for many kinds of diseases, and to screen safe and effective prescription for lipid reduction has clinical significance.OBJECTIVE: To study the influence of puerarin-arginine composition on lipid metabolism in rats.DESIGN: Comparative observing study based on animal model.SETTING: Experimental animal center and department of biochemistry in a military hospital of Chinese PLA.MATERIALS: This study was carried out at the Experimental Animal Center and Department of Biochemistry of the General Military Hospital of Chinese PLA between May and August 2004. Eighty Wistar rats were randomly divided into high dosage, middle dosage, low dosage group and control group with 10 male and 10 female rats each.METHODS: Rats took food freely, and experimental groups were supplemented with puerarine-arginine composition of different dosage (0, 0.93,1.39 and 1.85 g/kg) for observing the influence on rats.MAIN OUTCOME MEASURES:① blood parameters; ② change of body mass and utilization rate of food; ③ parameters of vital organs;④histopathological changes.RESULTS: From onset of the second week, body mass of both male and female rats in experimental groups were found obviously lower than those in control group. The levels of blood cholesterol in female rats were(1.63 ±0.20) and (1.29 ±0.23) mmol/L in 0.93 g/kg dosage group and control group respectively, and were(1.75 ±0. 13) and(1.31 ±0. 19) mmol/L in male rats,and both have statistical significance( F = 6.43, 6. 58, P ＜ 0. 05).Fatty parameters of the three experimental groups were found obviously lower as comparing with those of control group.CONCLUSION: Such composition has been proved to be able to exert function in lipid reduction by decreasing body mass, and improving utilization rate of food and proportion of cholesterol and organic fat.

Objective: To investigate the function of cytokine in the patients suffered from chronic Keshan disease. Methods: Serum IL-6, INF- α was tested among 55 patients of chronic Keshan disease and 30 health controls by the methods of ELISA, read in the unit of pg·mL-1. Results: The cytokine was significantly higher in Keshan disease patients than that in health control group. Conclusion: Serum levels of IL-6, TNF- α is higher in chronic Keshan disease patients than in health control group. It was showed that cytokine plays an important role during the etiopathology and the development in Chronic Keshan disease.

Acetylation of lysine residues of core histone N-terminal domains has been found correlatively associated with transcriptional activation in eukaryotes. Recent studies showed that some transcriptional regulators with histone acetyltransferase(HAT) and deacetylase(HDAC) activities may play a causative role in regulating gene expression. The link between tumorigenic processes and misregulated or mistargeted HAT and HDAC activities has been reported.

Objective To investigate the effect of angelica polysaccharide (APS) on bioactivity of bone marrow macrophage (BMM?) and its relationship to hematopoietic regulation, for clarifying the hematonic mechanism of Angelica sinensis. Methods The techniques of hematopoietic progenitor cell culture and BMM? culture in vitro, biological assay of hematopoietic growth factor (HGF) in culture media of BMM?, immunocytochemistry, and nucleic acid in situ hybridization were used. Results The culture supernatant of BMM? induced by APS can enhance the CFU-Mix, CFU-E, CFU-GM; the expression of erythropoietin (EPO), GM-CSF, IL-3, IL-6 protein in BMM? induced by APS was much stronger than that in the control group at different levels; the expression of EPO mRNA and GM-CSF mRNA in BMM? induced by APS were intensified. Conclusion APS may directly and/or indirectly stimulate the BMM? in hematopoietic inductive microenvironment to accelerate the synthesis and secretion of hematopoietic regulation factors on the basis of gene and protein level, such as EPO, GM-CSF, IL-3, IL-6, which in turn to promote the proliferation and differentiation of CFU-Mix, CFU-E, and CFU-GM.

In this experiment, it took the rat renal ischemic model with right nephrectomy and occlusion of the left renal pedical for 60 minutes. The effects of ischemia, reperfusion, allopurinol (AP) and superoxide dismutase(SOD) on this ischemic model were observed. The changes of serum creatine(Scr), renal coefficient(RC) and renal morphology were studied. In the reperfusion group which had sufferred from 60 minutes ischemia followed by 24 hours reperfusion, the Scr level was much higher than that in the pure isehemic group(P

Objective To observe the clinical therapeutic effects of Yishen-Jianpi-Huoxue Decoction on the adolescent patients with Polycystic Ovary Syndrome (PCOS).Methods 42 patients diagnosed of PCOS of spleen and kidney deficiency syndrome were recruited in to traditional Chinese medicine group and treated with Yishen-Jianpi-Huoxue Decoction,and 35 patients of PCOS were recruited into control group and guided with the healthy lifestyle.The course of the treatment was 3 months.The menstrual cycle,body mass index (BMI),F-G score,GAGS score,basal body temperature (BBT),and endocrine related inspection were analyzed before and after the treatment.Results The clinical symptoms and related laboratory tests were improved in both groups after the treatment,but the effects of traditional Chinese medicine group was much better than the control group in BMI(23.11+3.64),GAGS score(7.33±7.06),FINS(14.73+8.97) mmol/L,and HOMA-IR (3.13 ±1.94),P＜0.05 or ＜0.01.Conclusion Yishen-Jianpi-Huoxue Decoction can improve the clinical symptoms and endocrine condition of edolescent PCOS patients.