Objectives: To explore the destructive mechanism of P.gingivalis on periodontium and to better understand the pathogenic effects of P.gingivalis fimbriae. Methods:Western blotting was used to detect the degradation effects of P.gingivalis ATCC 33277 and its fimA-deficient mutant on focal adhesion components paxillin and focal adhesion kinase (FAK) of Hela cells and immortalized gingival epithelial cells (IHGE cells). Results:P.gingivalis ATCC 33277 wild strain and its fimA-deficient mutant induced degradation of paxillin and FAK both in Hela cells and in IHGE cells. fimA-deficient mutant had a remarkable weaker degradation ability than the wild strain. In IHGE cells, paxillin and FAK were degraded in a time-and MOI-dependent manner. Conclusion:P.gingivalis fimbriae-mediated adhesion and invasion to epithelial cells may promote the degradation of focal adhesion components. IHGE cells may be more suitable for the study of periodontal pathogens than Hela cells.

Objective:To study the effects of green-tea polyphenol, (epigallocatechin gallate,EGCg) on the cellular inflammatory responses of gingival epithelial cells. In order to find out a safe and efficient inflammation-inhibitor for periodontitis prevention and treatment. Methods:A model of cellular inflammatory responses of gingival epithelial cells stimulated by Porphyromonas gingivalis vesicles in vitro was established. The effects of EGCg on PGE2 production of gingival epithelial cells was detected by ELISA. Further more, the effects of EGCg on COX (cyclooxygenase)-2 and MMP (matrix metalloproteinase)-3 mRNA expression were determined by Real-time RT-PCR. Results:EGCg dose-dependently inhibited PGE2 production and COX-2, MMP-3 mRNA expressions. Conclusion:EGCg has inhibitory effects on cellular inflammatory responses of gingival epithelial cells and possesses the potentiality to be a periodontal inflammation-inhibitor.

This paper elucidates the diagnosis and treatment of phlegm syndrome in Jing-Yue Complete Works(Jing Yue Quan Shu from pathogenesis,syndrome differentiation,principle and method of treatment,recipe,medicines,diet and rest,which are used for reference in the modern clinical diagnosis and treatment of traditional Chinese medicine.The diagnosis and treatment of phlegm syndrome in Jing-Yue Complete Works(Jing Yue Quan Shu) is widely used in internal medicine,surgery,gynecology and pediatrics,etc.Based on the point of emphasizing yin-yang in person,Zhang Jingyue supposed that the diagnosis and treatment of phlegm syndrome should focus on spleen and kidney,strengthening the body resistance,treating symptoms in acute condition,choosing recipe and medicines according to the position and character of phlegm,diet and rest.

Traditional Chinese Medicine has its own academic thoughts and practical experience on diagnosing and treating phlegm disease,which has sprouted in Qin and Han Dynasties,originated in Jin and Tang Dynasties,developed in Song and Yuan Dynasties,become prosperous in the Ming and Qing Dynasties and made much progress in the recent years.This article shows researches of academic thoughts and clinic experiences on diagnosing and treating phlegm disease,which is based on ancient books and records of Traditional Chinese Medicine from Pre-Qin Period to the Sui and Tang Dynasties.Hope to provide beneficial reference to modern clinic,health preservation,prevention and treatment of phlegm disease.

During the Ming dynasty,great progress had been made on the theoretical and clinical aspects of diagnosis and treatment of phlegm disease in TCM.It mainly showed that: during this period,unprecedented monographs about phlegm diseases such as 《Tan Huo Zhuan Men》,《Tan Huo Dian Xue》 appeared.The ancient medical scholars all paid attention to the in? uence and harm of the phlegm to the body and accumulated abundant experience in clinical diagnosis and treatment,further made a great summary to provide reference to the posterity.In this article,several physicians’ academic thoughts of phlegm disease were chosen to research.The theories and experience of ancient medical scholars were explained.It has important theoretical and practical values for the health preservation and clinical diagnosis and treatment.

The physicians of Qing Dynasty made deeper knowledge to the formation and harm of the phlegm and systematically summarized the theory of the diagnosis, syndrome differentiation and treatment of the phlegm diseases. In the literatures of such as CHEN Shi-duo, LIN Pei-qin, HE Meng-yao, YOU Zai-jing, the special discussion of the phlegm diseases diagnosis and treatment which contents were theory, principle, prescription and drugs. Next, in the surgery, ophthalmology, gynecology, pediatrics, the physicians paid attention to the diagnosis and treatment from the phlegm and summarized the clinical practical experience. Moreover, in the Chuan Ya Nei Bian, Chuan Ya Wai Bian written by ZHAO Xue-min, the experience of "Zou Fang Yi" on treating the phlegm disease by the method of Ding, Chuan, Jie had its own characteristics.

MinK-related peptides is a unique family of ion channel ancillary subunits,that modify voltage-gating,conductance and pharmacology of ion channel subunits.This article reviews MinK-related peptides on the gene and protein structure,subunits from a variety of subfamilies,and the potential role in physiology and pathology.

Objective:To detect and compare the intensity of gingipain K(Kgp)in culture medium and cell extract of Porphyromonas gingivalis(P.gingivalis)isolates in puberty gingivitis,and then to reveal the possible relationship between Kgp and puberty gingivitis.Methods:36 patients with puberty gingivitis aged from 14 to 17 years were enrolled.Clinical parameters including GI,SBI and PD were evaluated before subgingival plaque samples collection.Subgingival plaque samples were collected and then P.gingivalis isolates were obtained.16S rRNA PCR was used to confirm the presence of P.gingivalis in clinical isolates.Bacteria were cultured in BHI agar base and harvested at the end of log-phase growth.Culture fractions of P.gingivalis(culture medium and cell extracts)were performed with SDS-PAGE and Western blot technique using primary antibody against specific anti-Kgp N-terminal IgG subdomain.The data were statistically analyzed using SPSS 11.5 software.The relationship between the Kgp intensity and the clinical parameters was statistically analyzed using sum rank test.Results:There was positive correlation between the intensity of Kgp N-terminal IgG subdomain and the clinical parameters(P

Objective To evaluate the efficacy of lamivudine and adefovir in the treatment of patients with decompensated hepatitis B cirrhosis . Methods 90 cases of patients with hepatitis B cirrhosis of the liver decompensation period from August 2013 to June 2015 in our hospital were researched.According to the random number table method divided into observation group and control group , each of 45 cases, the total course of treatment was one year.The observation group was treated with lamivudine combined with adefovir dipivoxil , control group was treated with lamivudine.The clinical effect, the indexes of liver function, the changes of HBV-DNA and Child-pugh score were compared between the two groups after treatment.Results After treatment, the total effective rate of the observation group ( 93.33%) was obviously higher than that of control group (48.89%), the difference was statistically significant (P<0.05), the observation group alanine amino acid, total bilirubin, aspartate aminotransferase were (71.23 ±21.32)U/L, (28.32 ±4.65)Umol/lL, (4.65 ±9.25)U/L, they were significantly lower than control group (111.54 ±16.25)U/L, (46.53 ±4.89 ) Umol/L, ( 4.89 ±12.11 ) U/L, the observation group albumin ( 39.82 ±2.62 ) g/L was significantly higher than control group (35.55 ±2.22)g/L, the difference was statistically significant (P<0.05), after treatment, the observation group hepatitis b HBV DNA levels, Dhild pugh score (2.78 ±0.45)log10 copies/mL, (6.12 ±1.23) were significantly lower than the control group (3.89 ±0.65)log10 copies/mL, (7.89 ± 1.21)scores, the difference was statistically significant (P <0.05).Conclusion The combination of lamivudine and adefovir dipivoxil significantly decompensated hepatitis B cirrhosis has a curative effect, it could effectively improve the patient's liver function, Dhild-pugh score, HBV-DNA level.