Objective To observe the clinical therapeutic effects of Yishen-Jianpi-Huoxue Decoction on the adolescent patients with Polycystic Ovary Syndrome (PCOS).Methods 42 patients diagnosed of PCOS of spleen and kidney deficiency syndrome were recruited in to traditional Chinese medicine group and treated with Yishen-Jianpi-Huoxue Decoction,and 35 patients of PCOS were recruited into control group and guided with the healthy lifestyle.The course of the treatment was 3 months.The menstrual cycle,body mass index (BMI),F-G score,GAGS score,basal body temperature (BBT),and endocrine related inspection were analyzed before and after the treatment.Results The clinical symptoms and related laboratory tests were improved in both groups after the treatment,but the effects of traditional Chinese medicine group was much better than the control group in BMI(23.11+3.64),GAGS score(7.33±7.06),FINS(14.73+8.97) mmol/L,and HOMA-IR (3.13 ±1.94),P＜0.05 or ＜0.01.Conclusion Yishen-Jianpi-Huoxue Decoction can improve the clinical symptoms and endocrine condition of edolescent PCOS patients.

At present, due to the opening and circulation of salt market, potassium iodate iodized salt, potassium iodide iodized salt, seaweed iodized salt and non-iodized salt may exist simultaneously in the edible salt market of a region, which brings new problems to the work of testing iodine content of edible salt in the laboratory. In order to carry out salt iodine detection for prevention and monitoring of iodine deficiency disorders, this paper introduces some methods which established by the author for a rapid qualitative, semi-quantitative and quantitative detection of iodized salt of different forms of iodine and non-iodized salt. And further application and discussion of the methods are described for reference of iodized salt monitoring.

Objective To analyze the relationship between BIDP and breast cancer, and to evaluate the treatment of BIDP. Methods 103 cases of BIDP were studied retrospectively in respect of its clinical and pathological features, diagnosis, and treatment. Results Among the patients, there were 102 women and 1 man. The diagnosis was all verified by pathological examination. The rate of misdiagnosis was high(62.1%). Local excision was performed in 18 patients, regional lobular excision in 62, in nine patients modified radical mastectomy for malignant change in 9 patients, and simple excision for malignancy in 2 patients. The recurrent rate after local excision was 11.7% after 13 years, and the rate of malignant change was 6.8%. Conclusions To sum up the relationship and differentiate method between BIDP and Breast Cancer, and briefly discuss the methods of treatment.

Objective: The configuration of fixed assets in hospital, should meet the need of diagnosis, treatment and daily operation. Methods: By considering the input, output and evaluation of fixed assets in the management process, which related to the medium and long term development, we find a way to establish the information platform of fixed assets, which actually conforms to the hospital operation. Results:Effectively, the management of fixed assets was lead to a virtuous cycle. Conclusion: In the management process, economic benefits management, performance and quality management and evaluation should be different among different kinds of fixed assets. Thus, the hospital managers should focus and treat them differently, in order to achieve better or more effective management.

Objective To screen the suitable sample digestion method for measuring iodine in serum by arsenic cerium catalytic spectrophotometric method , and to carry out the methodology test for the newly developed arsenic cerium catalytic spectrophotometry for determination of iodine in serum .Methods Methodology evaluation.Experimentswere on the sample on the sample digestion method of serum iodine with several common digestive agents . After digestion treatment , the concentration of iodine in serum was determined by arsenic cerium catalytic spectrophotometry .The linearity and linear range of the standard curve, detection limit, precision, recovery rate and anti -interference ability of the newlydeveloped arsenic cerium catalytic spectrophotometry were tested .Results Sample pretreatment method included in the newly developed arsenic cerium catalytic spectrophotometry was selected as follows: perchloric acid -sodium chlorate solution was used as the digestive agent to digest 120 min at 130℃.The calibration relation of C =a+blgA( C: iodine concentration ,A: measuring absorbance ) of the newly developed method existed when arsenic ceriumcatalytic reaction was kept at a certain stable temperature range from 13℃ to 30℃ and in certain stable reacting time .The linear range of the calibration curvewas 0 -300 μg/Land the linear correlative coefficient was -0.9996 --0.9999.The intra assay coefficients of variation ( CVs ) were 0.70%,0.70%and 0.74%(n=6), and the inter assay CVs were 0.57%, 0.51% and 0.57% (n=6) for 3 serum samples.The average recovery was 97.9% with a range from 92.3% to 105.8% for 3 serum samples.Conclusions The newly developed arsenic cerium catalytic spectrophotometry has good precision and accuracy .The sample digestion agent is easy to be prepared and reserved , and the instrument is simple and easy to be operated .The method can be widely used as a reliable technique for measuring iodine in serum.

Objective To establish a catalytic spectrophotometric method for determination of iodine in water using the same arsenious acid solution and ceric ammonium sulfate solution as those used in the 2016 version standard method for determination of urinary iodine,and to meet the needs of wide concentration range of water iodine detection.Methods After pretreatment of the water sample with the effective chlorine of sodium dichloroisocyanurate solution for eliminating the interference of reducing substances at room temperature,the concentration of iodine in water was determined by arsenic cerium catalytic spectrophotometry using the same 0.025 mol/L arsenious acid solution and 0.025 mol/L ceric ammonium sulfate solution as those used in the 2016 version standard method for determination of urinary iodine.The linear relationship of the standard curve and the linear rang of different iodine concentration range (0-100,0-400,0-800μg/L),the detection limit,the precision and the accuracy of the sample were tested.Results The calibration relation of C =a + blgA (C:iodine concentration,A:measuring absorbance) in the new method existed when arsenic cerium catalytic reaction was kept at a certain stable temperature ranging from 15 ℃ to 30 ℃ in certain stable reacting time.The linear correlative coefficients absolute value of different iodine concentration range (0-100,0-400,0-800 μg/L) were all greater than 0.999 0,corresponding to the water iodine detection limits were 0.3 μ,g/L (sample volume of 0.80 ml),1.2 μg/L (sample volume of 0.20 ml),and 2.2.μg/L (sample volume of 0.10 ml),respectively.The coefficients of variation (CV) of the three different iodine concentration range were all below 1.0％ (n =6).The iodine recovery rate range of a total of 10 different water samples in these three different concentration range was 95.8％-98.7％,98.3％-103.7％ and 98.5％-104.5％,and the average recovery rate was 97.6％,100.4％ and 102.4％,respectively.In the range of these three different standard curves,water iodine standard materials GBW09114c,GBW09114a and GBW09113c were measured.The relative errors between the results and the given values were ＜ 3.0％,which were in the range of uncertainty of the given value.Conclusion This method verified by methodology experiments has wide linear range,high precision,accuracy,and anti-interference ability,good reproducibility,and is easy to operate,with reduced amount of arsenic waste,reduced environmental pollution,and is suitable for application in different areas to determine water iodine.

Objective To investigate the ultrasonographic performance of infantile hepatic hemangioendothelioma(IHHE).Methods Clinical and ultrasonographic findings in 14 cases with IHHE were reviewed retrospectively,and compared with CT and MR.Results Among 14 cases,there were 6 cases with single mass,5 cases with multiple masses and 3 cases with diffuse masses.Ultrasonography displayed single mass and multiple masses with clear boundary,heterogeneous inner echo,calcification was detected in 4 cases and anechoic areas were detected in 6 cases.Diffuse masses were small,ill-defined,the echogenicity of the whole liver was extremely disorder.The hepatic artery and the proximal abdominal aortic dilatation was found in 4 cases,and the abdominal aorta which below the coeliac artery became relatively thinner in these cases.Color Doppler showed rich blood supply and intratumoral vasodilation in 8 cases,PW detection of intratumoral arteriovenous fistula in 4 cases.Conclusions Ultrasonography findings of IHHE have some characteristic feature.

OBJECTIVE:To establish the quality standards for Fufang ningshen granule. METHODS:TLC was used for the qualitative identification of Radix angelicae and White paenoy;HPLC was adopted for the contents determination of spinosin and verbascoside:the column was Kromasil C18 with the mobile phase of acetonitrile-0.1% acetic acid(16∶84,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temerpature was 30 ℃. RESULTS:The TLC spots of R. angelicae and W. pae-noy were clear with good separation,negative control without interference. The linear range was 0.055 44-0.277 2μg for spinosin(r=0.999 4)and 0.055 98-0.279 9 μg for verbascoside(r=0.999 5);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 95.62%-100.53%(RSD=1.77%,n=9) and 95.63%-102.57%(RSD=2.74%,n=9). CONCLU-SIONS:The standard can be used for the quality control of Fufang ningshen granule.

Objective To explore whether Helq deletion affect the pluripotency of stem cells. Methods Helq knockout embryonic stem cells were obtained by CRISPR?Cas9 gene editing technique. Results The results of immunoflu?orescence analysis showed that the expression of Oct4 and Nanog had no obvious difference to that of the control cells. The Helq-/ - embryonic stem cells could produce viable pups by tetraploid complementation, indicating that their pluripotency was not affected. Meanwhile, we found that day 2 epiblast?like cells also were obtained through differentiation of the Helq-/ - embryonic stem cells in vitro. Immunostaining and real?time PCR analysis showed that the gene expression of Helq-/ - epiblast cells were similar to the wild type cells. Conclusions Taken together, it is proved that the genomic in?stability caused by Helq deletion does not affect the pluripotency of pluripotent stem cells.

Owing to medical students'joining in, the correlation between doctors and patients have expanded into the relationship between doctors and medical students and patients in clinical teaching of sexually transmitted disease ( STD). It is easy to disobey ethical principle and lead to medical dispute happened if not be cautious to deal with the relationship. So we present that ① strictly to following medical ethical principle; ② establishing a harmonious doctor - patient relationship; ③improveing teaching ways to a rational extent in medical treatment of medical students were concerned with patients, and disposing the relationship between " Teaching" and " Learning" ; ④enhancing the professional morality culture of doctors( clinical teachers) and medical students, and providing the high quality service for patients. Thus ethical problems can be avoided effectively in clinical teaching of STD, and clinical teaching can be ensured go on wheels effectively too.