1.Analysis of Internet Addiction and Relevant Prevention & Treatment
Chinese Medical Ethics 1994;0(06):-
The Internet addiction has become a heated social issue which seriously affects individual's mental health.This paper analyzes the hazards and forming causes of Internet addiction from psychological and ethical perspectives.The prevention,society-assisted treatment & family-assisted treatment,and clinical medication therapy are come up with as countermeasures.
2.Treatment of nonunion of tibial fractures with local muscle flap transfer and injection of autogenous bone marrow
Ruimin WANG ; Yaozhong WANG ; Lei ZHAO ; Zhidong WANG ; Lifeng CAI
Chinese Journal of Orthopaedic Trauma 2004;0(05):-
Objective To report the treatment outcome of nonunion of tibial fractures with local muscle flap transfer and injection of autogenous bone marrow. Methods 15 patients who had suffered from nonunion of tibial fracture 10 to 42 (mean 22) months after the initial injury underwent open reduction, internal fixation with locking intramedullary nail, wound coverage with locally transferred muscle flap and injection of autogenous bone marrow into the fracture site 12 to 15 days after operation. Results The follow-ups revealed bone union in all the cases of this series with a mean healing time of 22 (3 to 11) months. Except for limited necrosis of the skin edge in 2 cases which healed after dressing changes, the wounds healed primarily in all the cases without infection and implant failure. Conclusion Besides stable internal fixation and bone graft, coverage of fracture site with locally transferred muscle flap and injection of autogenous bone marrow can be used to treat nonunion of tibial fractures with satisfactory results.
3.Stereotactic mammography surgical biopsy in the diagnosis of occult breast cancar
Dingcun LUO ; Xiaoyang LI ; Yaozhong NI ; Haibin ZHOU ; Lan WANG
Chinese Journal of General Surgery 2000;0(12):-
Objective To investigate the value of surgical biopsy with guidance of indwelling wire through stereotactic puncture in the diagnosis of occult breast cancer (OBC) and the clinical usage. Method Surgical biopsy was performed on 22 patients with suspected nonpalpable breast lesions. ResultsSurgical biopsy, including localization and resection of the lesion, under the guidance of a guide wire was successful with single procedure for all 22 lesions. The accuracy of qualitative diagnosis was 100%. Pathological examination showed malignant in 9 lesions and benign in 13 lesions. ConclusionThe surgical biopsy with the guidance of indwelling wire through stereotactic puncture is valuable technigue for the diagnosis of occult breast lesions.
4.Simultaneous HPLC Determination of Hesperidin, Magnolol, Honokiol and Liquiritin in Soft Capsule Jia-Wei Huo-Xiang Zheng-Qi
Yanfei CAO ; Zhenzhong WANG ; Yuan BI ; Zhengkuan WANG ; Yaozhong LYU ; Qin WAN ; Wei XIAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(8):1768-1771
This study was aimed to develop an HPLC method for the determination of hesperidin,magnolol,honoki-oland liquiritin in Soft Capsule Jia-Wei Huo-Xiang Zheng-Qi (JWHXZQ). AKromasil C18 column (250 mmí4.6 mm, 5 μm) was used with water-methanol as mobile phasegradient elution. The flow rate was 1.0 mL·min-1, and the de-tecting wavelength was at 287 nm. The results showed that the linearity ranges ofhesperidin,magnolol,honokioland liquiritinwere 4.47-178.70 μg·mL-1, 3.42-136.96 μg·mL-1, 3.49-139.48 μg·mL-1, 3.92-157.20 μg·mL-1, respec-tively (r>0.999). The average recoveries of them were 99.48%, 99.05%, 99.57% and 99.79%, respectively. It was concluded that the method was accurate, sensitive and specific for quality control of Soft Capsule JWHXZQ.
5.Precursor T-lymphoblastic lymphoma of the breast: one case report and reviews of literature
Xiaofan LIU ; Yaozhong ZHAO ; Yafei WANG ; Ying WANG ; Dehui ZOU ; Linsheng QIAN ; Lugui QIU
Journal of Leukemia & Lymphoma 2008;17(2):110-113
Objective To improve the recognition of precursor T- lymphoblastic lymphoma (T-LBL)of the breast. Methods One case of breast T- LBL was reported, the development of breast non-Hodgkin lymphoma and T-LBL presenting as breast masses was reviewed. Results One case of breast T-LBL was diagnosed, the patient with a high leukocyte count, breast mass and peripheral lymph nodes was treated with Hyper-CVAD regimen after active therapy and achieved complete remission (CR). Followed by maintenance therapy with Hyper-CVAD regimen for three times, disease free survival had been obtained five month.Conclusion Lymphoma of the brest is a rare malignancy. T-LBL is a highly aggressive disease with adverse prognosis, it is very uncommon for presenting as lymphoma of breast. Initiation of intensive muhiagent chemotherapy can improve the free survival and the prognosis.
6.Apolipoprotein C3 enhanced THP-1 cell adhesion to aortas of mice
Yaozhong XU ; Juan YU ; Yisong XIONG ; Shaopeng CHU ; Huimin WANG ; Renqian ZHONG
Chinese Journal of Microbiology and Immunology 2013;(2):99-102
Objective To investigate the enhancing effect of apolipoprotein C3 (APOC3) on THP-1 cell adhesion to aortas of mice.Methods Microsurgery was performed to separate the aorta of C57BL/6 mice in sterile condition.After stimulated by APOC3 (100 △g/ml) in vitro for 16 h,the aorta was allowed to adhere for 1 h with CFSE labeled THP-1 cells (1 ×106/ml).Then the adhesion effect was observed,and the expressions of vascular adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) were detected by immunohistochemical method.Results Adhesion effect of the mice aorta with THP-1 cells in the APOC3 stimulated group was stronger than the control group.Both the expressions of VCAM-1 and ICAM-1 in aortas were increased by APOC3,but the former was significantly up-regulated than the latter.Conclusion Apolipoprotein C3 could enhance THP-1 cell adhesion to aortas of mice.
7.Effects of different medications of fentanyl during anesthesia induction on fentanyl-induced cough
Chao DING ; Li SUN ; Yan ZHANG ; Hai WANG ; Yaozhong CHENG ; Guijun ZHAO
Chinese Journal of Anesthesiology 2011;31(9):1073-1075
Objective To compare the effects of different medications of fentanyl during anesthesia induction on fentanyl-induced cough.Methods Four hundred and twenty ASA Ⅰ or Ⅱ patients aged 18-60 yr undergoing selective operations under general anesthesia,were randomly divided into 4 groups ( n =105 each):group Ⅰ (control group) received successive intravenous injection of midazolam 0.05 mg/kg,fentanyl 2 μg/kg,propofol 2 mg/kg,and rocuronium 1 mg/kg,group Ⅱ (pre-injection group) received successive intravenous injection of midazolam0.05 mg/kg,fentanyl 0.5 μg/kg,propofol 2 mg/kg,rocuronium 1 mg/kg,and fentanyl 1.5 μg/kg,group Ⅲ (dilution group) received successive intravenous injection of midazolam 0.05 mg/kg,fentanyl 2 μg/kg (20 μg/ml),propofol 2 mg/kg,and rocuronium 1 mg/kg,and group Ⅳ (last injection group) received successive intravenous injection of midazolam 0.05 mg/kg,propofol 2 mg/kg,rocuronium 1 mg/kg,and fentanyl 2 μg/kg.Fentanyl concentration was 50 μg/ml in each group except group Ⅲ.Endotracheal intubation was performed 2 min after anesthesia induction.The incidence and severity of fentanyl-induced cough before intubation were recorded and the incidence of propofol-induced pain was recorded.Invasive arterial blood pressure (ABP) and heart rate (HR) were observed before induction (T1 ),immediately after induction (T2 ),at time of coughing (T3 ),and at time of endotracheal intubation (T4).Results ABP and HR had no significant differences at T1,T2,T3,and T4between the four groups (P > 0.05).The incidence of propofol-induced pain had no significant differences between the four groups (P > 0.05).The incidences of cough was 7.6% in group Ⅱ,9.5% in group Ⅲ,and 1.9% in group Ⅳ,which were significantly lower than 35.2% in group Ⅰ ( P < 0.01).The incidence of cough in group Ⅳ was significantly lower than that in groups [ and Ⅲ (P < 0.05).In the four groups,ABP and HR were significantly higher at T3 than that at T1 and T2 ( P < 0.01 ).Conclusion Different medications of fentanyl including last injection,pre-injection,and dilution of fentanyl can significantly reduce the incidence of fentanyl-induced cough during anesthesia induction,and injection has the best effect.
8.Rapid Identification of Candida albicans and Candida dubliniensis Isolates With Two Specific Probes Si-multaneously
Liping ZHU ; Qiangqiang ZHANG ; Yaozhong SHI ; Li LI ; Jiajun WANG ; Xinhua WENG
Chinese Journal of Dermatology 1994;0(05):-
Objective To develop a microtitration plate en zyme immunoassay to differentiate Candida albicans from Candida dubliniensis isolates using two specific probes s imultaneously.Methods The fun-gus-specific universal primers derived from the internal transcribed s pacer region of fungal rDNA were labeled with biotin,while the C.albicans or C.dubliniensis specific capture probes were coated on the microplates.Genomic DNA purified from the two species was amplified by PCR.The biotinylated p roducts were captured by the probes coated on the microplates.The A 405 value was finally determined by the c olorimetric assay.Results The two species of Candida could be detected specifically.Out of 108clinical isolates originally identified as C.albicans on the basis of germtube formation,two isolates were positive for C.dubliniensis and negative for C.albicans.The other106isolates were positive for C.albicans and negative for C.dubliniensis.Conclusions Two-specific-probe hybridization method is rapid and re liable for differentiating C.albicans from C.dubliniensis.
9.Efficacy of bisphosphonates in reducing skeletal events in patients with multiple myeloma
Sidan LI ; Yan XU ; Gang AN ; Yafei WANG ; Yaozhong ZHAO ; Dehui ZOU ; Lugui QIU
Journal of Leukemia & Lymphoma 2012;21(7):397-400
Objective To review the efficacy of bisphosphonates in reducing skeletal events in patients with multiple myeloma. Methods Two hundred and five patients with newly diagnosed MM were enrolled in this retrospective study,with bisphosphonates or not.Skeletal-related events,therapeutic reaction of myeloma bone disease and patient survival were analyzed. Results The occurrence of skeletal-related events (SRE) per patient year (P<0.01) and the time to first SRE (P<0.05)were significantly lower in the reatment group than in the untreated group. After 6 cycles of treatment, a significant higher percentage of effective and marked effect patients were observed through X ray in the treatment group (80.0 %) compared to the untreated group (48.7 %), P<0.001. There was no overall significant difference in the level of serum calcium between the two groups (P=0.278). After 6 cycles of treatment, the patients who received bisphosphonates had significant decreases in bone pain and lower ECOG score (ECOG≤2) compared to the untreated group (P<0.05). Bisphosphonates were tolerated well, and the common adverse reaction including gastrointestinal reaction (3 cases,3.3 %),fever (lcase,1.1%) and skin rash (2 cases,2.2 %).There was no significant difference in overall survival between the two treatment groups,(P=0.580).Conclusion Infusions of Bisphosphonates could reduce the occurrence of skeletal- related events (SRE), prolong the time to first SRE and improve the quality of life of patients with multiple myeloma. Bisphosphonates could not prolong survival time of myeloma patients.
10.Report of nine cases of aggressive natural killer-cell leukemia
Gang AN ; Dehui ZOU ; Yafei WANG ; Yaozhong ZHAO ; Lugui QIU ; Junyuan QI
Journal of Leukemia & Lymphoma 2009;18(2):83-85
Objective To identify the clinical and pathological features of aggressive natural killercell leukemia (ANKL). Methods 9 cases of ANKL fulfilling the criteria defined by the World Health Organization classification were retrospectively analyzed with literature review, Results Systemic symptoms, hepatomegaly, splenomegaly, lymphadenopathy were frequently observed. Liver dysfunction, neutropenia, anemia and thrombocytopenia were often seen during the course of the disease. Most of the bone marrow shows focal or subtle infiltration by the neoplastic cells. The immunophenotype of cells was characteristic for CD+56, sCD-36, and variable expression of CD2, CD7, CD8 and CD11b. T-cell receptor (TCR) genes rearrangement were in germline configuration. Median survival time was 9 weeks. Conclusion ANKL is an entity of mature cytotoxic NK-cell neoplasms with distinct phenotype and disease presentations. The disease often has a fulminant course with a poor response to chemotherapy and a short survival time. Patients achieving CR showed significantly longer survival time, but the remission did not translate into cure of the disease.