Limiting dilution assay and ~(51)Cr-release assay were used to quantitate the pre-cursors of MAF-secreting T cells in lymph nodes or lymphoblast population of themice infected with Leishmania major(Lm).At lst and 4th week after infectionwith live Lm,the numbers of MAF-P in lymph nodes of resistant mice(CBA/T6)and susceptible mice(BALB/C)showed no significant difference but resistantmice had much more MAF-P than susceptible mice at 8th week.The effect ofantigen,interleukin-2 and irradiated syngeneic spleen cells on the detection of MAF-P were investigated、It was found that syngeneic spleen cells were an impor-tant factors for the assay of MAF-P of lymphoblasts but lymph nodes cells,and that the addition of both antigen and interleukin-2 can significantly increasethe number of MAF-P than antigen or interleukin-2 alone when the cells oflymph nodes were examined.

Molecular immunologic technique gets quick development in recent years, especially the fast development of the immuno-labelling technique, which found its indispensable status in the study of traditional Chinese medicine (TCM). With the development of the study of TCM, medical immunology connecting TCM with the Western medicine has become one of the most indispensable conjunctive subjects. Scientists can study the theories and prevention or treatment mechanisms of TCM not only at holistic and cell levels, but also at molecular and gene levels. Appropriate application of the molecular immunologic techniques according to their characteristics will play an important role in the study of Chinese integrative medicine.

OBJECTIVE:To compare the clinical effects between alprostadil and the traditional therapy in treating sudden deafness.METHODS:A total of120patients were randomly ascribed to receive alprostadil(treatment group)or traditional drugs(e.g.low molecule dextran,dexameth,danshen root injection,control group),the two groups were assisted with hyper-baric oxygen treatment,the total effective rates of the two groups were compared.RESULTS:The total effective rates of the treatment group and the control group were75%and56.7%,respectively,and there was significant difference between the two groups(P

Objective To analyze the related influence factors on the prognosis of hypertensive cerebral hemorrhage in basal ganglia with operation treatment,provide the basis for clinical prevention and treatment.Methods 80 patients for treatment of hypertensive cerebral hemorrhage were selected.By a retrospective study,we collected the clinical data and postoperative following -up data,status,complications,and observed the postoperative recovery.Sta-tistical different hypertension,preoperative breaking into the ventricle,disturbance of consciousness,operation time and preoperative hematoma volume of the prognosis.Results In 80 patients,48 cases(60.00%)of patients has good prognosis;moderate disability,taking care of themselves life 10 cases(12.50%);severe disability,life can not take care of 9 cases(11.25%);plant survival of 5 cases(6.25%)and 8 cases of death(10.00%).22 cases of patients with brain hemorrhage again,3 cases postoperative pulmonary infection,6 cases of secondary organ dysfunction.Poor prognosis of preoperative ventricle prognosis and disturbance of consciousness of patients was 88.26%(18 /23)and 63.64%(21 /33),which was higher than the preoperative without breaking into the ventricle and no disturbance of consciousness 24.54%(14 /57)and 36.36%(21 /33),the difference was statistically significant(χ2 =10.38,9.76, P <0.05).The timing of surgery,the prognosis of 48h patients was 69.23%(9 /13),which was higher than that of less than 6h and 6 48h patients,the difference was significance(χ2 =10.06,P <0.05).Patients with poor preopera-tive hematoma volume >60mL was 47.62%(20 /42),which was higher than the preoperative hematoma volume 40 -60mLpatients31.38%(12 /38),the difference was statistically significant(χ2 =9.12,P <0.05).Conclusion It has obvious effect on surgery operation prognosis of patients with hypertensive cerebral hemorrhage,including optimal time for operation;bleeding time,intraventricular hemorrhage,disturbance of consciousness and bleeding volume.If the clinical treatment in the prevention and treatment to improve the survival and quality of life of patients with cerebral hemorrhage.

Objective To analyze the prevalence of postpartum depression in China.Methods CNKI,Chongqing VIP database,CBMDISK,Pubmed databases were searched for articles about postpartum depression prevalence in China published during 1996～2012.The random effects model was employed.Results There were 84 articles which provided 52 776 samples,and 8390 cases of depressive symptoms were detected.The pooled prevalence was 14.7％(13.1％～16.3％),The prevalence of postpartum depression was different in different regions,municipality directly under the central government was the lowest 10.9％(8.7％～12.7％),county area was the highest 16.4％ (11.9％～22.2％).From the east of China to the central and to the west,the prevalence rate assumed the trend of escalation.The prevalence which evaluated by standard of EPDS ≥ 10 was higher than the prevalence evaluated by EPDS ≥ 13 and SDS ≥50.Conclusions The prevalence of postpartum depression is high in China.More attention should be paid to the central,western of China,urban,rural and other underdeveloped areas.

Objective To explore the effects of Stilnox on the blood pressure and quality of life(QOL)in pa- tients with hypertension and insomnia.Methods 80 patients with hypertension and insomnia were received am- lodipine(5mg qd)as control group and amlodipine(5mg qd)plus stilnox(10mg qn)as treatment group in visit order for 8 weeks'follow up.Values of blood pressure were recorded and QOL data were assessed at the beginning and end of therapy with questionnaire.Results Blood pressure was significantly reduced in both groups(P

To investigate the efficacy of Qiangxin Mixture in patients with congestive heart failure (CHF).

Effects of Lu Jiao Fang on myocardial fibrosis and plasma aldosterone (ALD) content in the rat with increase of pressure - loading were investigated for approach to the machanism of anti - myocardial fibrosis. Left ventricular mass index(LVMI), myocardial collagen Ⅰ, Ⅱ Ⅲ and plasma ALD content were observed. Results showed that LVMI in the model group was significantly higher than that in the sham operation group(P

AIM: To study effects of Fuyuanbunao Granules (Radix Ginseng Rubra, Fructus Crataegi, Radix Astragali, Herba Epimedii, Radix Salviae Miltiorrhizae, Rhizoma Alismatis, Rhizoma Acori Tatarinowii) on ATPase activities, MDA level in mouse brain, SOD activities in mouse blood and the quotient of brain. METHODS: On the mouse model of Alzheimer' disease toxified with AlCl 3, the effects of Fuyuanbunao Granules on Na + K + ATPase, Mg 2+ ATPase activities and MDA level in mouse brain, SOD activities in mouse blood were evaluated with spectroscopic analysis, and the weight of brain was measured with analytical balance. RESULTS: Compared with Alzheimer'disease model team, Fuyuanbunao Granules enhance the activities of Na +、K + ATPase, Mg 2+ ATPase, decease MDA level in the mouse brain and enhance the activities of SOD in blood. It also increases the weight of brain. CONCLUSION: It indicates that enhancing the activity of ATPase and deceasing peroxide level may be one of the mechanism for Fuyuanbunao Granules to prevent and cure Alzheimer' disease.

Objectives To identify the related knowledge,attitude and behavior of drug of Xi'an adolescents and provide the evidence for establishment of intervention measures. Methods A conve-nience sampling method was used to select 1224 adolescents from grade one of junior middle school to grade three of senior middle school in 4 middle schools in Xi'an. A set of questionnaires were used to col-lect the data on knowledge, attitude and behavior of drug. All data were analyzed by SPSS 11.5. Results Mean score of drug knowledge was (22.45±9.77) and mean score of attitude to drug was (58.82±6.85). A-mong the investigation objects, 16 people (1.4%) admitted having used drug. Conclusions The adoles-cents don't have sufficient knowledge for the relation between infectious disease and drug abuse, and the difficulty of detoxification. The adolescents had a positive attitude towards drug. The adolescents' behavior of using drug should be attached importance to by school and parents.