Objective To investigate the effect of video-assisted thoracoscopic surgery( VATS) in thoracic disease,and the feasibility to carry out VATS for basic hospital. Methods The data of VATS treatment were collected to compare the differences between study group and control group,and evaluate the the feasibility to carry out VATS for basic hospital. Results The operation time was (100. 75±22. 72) min, blood loss was (54. 27±26. 21) mL,postoperative drainage was (920. 67±171. 99) mL. The postoperative complications were fewer,post-operative hospital stay was shorter,incision time was shorter(P=0. 000) and pain scores was lower(P=0. 000) in study group than that in control group. Basic hospital has the capacity to conduct this technical. Conclusion VATS is feasible to carry out in basic hospital.

Objective To supply a scientific basis for laying out a unified standard of reference value of Chinese newborn boys' hemoglobin. Methods After the reference values of 5169 Chinese healthy newborn boys' hemoglobin tested in 78 areas were collected, a research was made on the relationship between the reference value of Chinese newborn boys' hemoglobin and altitude by using curvilinear regression analysis. Results As the altitude gradually increased, the reference value of Chinese newborn boys' hemoglobin gradually increased by index law, with significant correlation (R=0.601, F=43.05, P= 0.0000). One curvilinear regression model was given out: =176.9e 0.00008662x?25.3. Conclusion If altitude of a particular area is known, the reference value of Chinese newborn boys' hemoglobin in this area can be established by using regression model. According to the correlation between Chinese newborn boys' hemoglobin and altitude, China can be divided into three regions: Qinghai-Tibet Region, Central Region, Eastern Region.

Clostridium difficile is an important zoonotic intestinal pathogen, which is widely present in humans and a variety of animals. The ST11 type C. difficile is one of the most widespread and harmful subtypes in the world. As a large country in pig farming, China lacks efficient methods for detecting C. difficile of porcine origin, leaving hidden dangers for the prevention and control of C. difficile. The aim of this study was to develop a specific and sensitive double-antibody sandwich ELISA for the epidemiological investigation of ST11 type C. difficile of porcine origin. Firstly, a 97 kDa receptor binding domain (RBD) was expressed in a prokaryotic host and purified. A hybridoma cell line AE2D3 capable of stably secreting monoclonal antibody targeting the RBD was screened, and the antibody subtype was determined to be IgG2b (κ). Secondly, a double antibody sandwich ELISA method was developed, where the monoclonal antibody targeting the RBD was used as a detection antibody, and the rabbit polyclonal antibody was used as a capture antibody. The chessboard method was used to determine the matching concentration of the capture antibody and the detection antibody, the antigen coating conditions, the blocking conditions, the incubation conditions for detection antibody and samples to be tested, as well as the reaction conditions of HRP-conjugated and reaction conditions of TMB chromogenic solution. The negative cutoff OD₄₅₀ was 0.152, and no cross-reaction with 13 strains of non-ST11 type C. difficile was found. The minimum detection concentration of RBD was 8.83 ng/mL. This specific and sensitive double-antibody sandwich ELISA provides a reliable serological detection method for epidemiological investigation of the ST11 type C. difficile in pig industry.
Animals ; Antibodies, Monoclonal ; Bacterial Proteins/genetics* ; Bacterial Toxins ; Clostridioides difficile ; Enzyme-Linked Immunosorbent Assay ; Hybridomas ; Swine