In recent years,several studies have revealed that diabetic patients may have decreased mortality compared with non-diabetic patients from acute hyperglycemia in intensive care units (ICUs),and the patients without diabetic history are most likely to benefit from intensive insulin therapy.This probably suggests a potential "protective" effect in diabetic patients with critical illness.Possible "protective" mechanisms include the adaptive reaction to chronic oxidative stress state in diabetes,the decreased incidence of acute complications followed by critical illness,and some non-biological factors.Thus much more attention of glycemic management should be paid to those hyperglyemic patients without diabetic history in ICUs.

Objective To investigate whether polypeptide cSN50,as a transmembrane peptides,can inhibit NF-κB nuclear translocation and its downstream gene expression to play a role to protect cells by blocking the combination of NF-κB with the importinα3 during the alcohol and endotoxin-induced inflammation and apoptosis.Methods Flow cytometry method was used to observe the apoptosis rate of HepG2 by different concentration of alcohol and/or endotoxin.Spectrophotometer method was used to detect Caspase-3 activity.TNF- α in cell culture supernatant was detected by ELISA assay.p50,importinα3,and IκBαunder the stimulation of alcohol and/or endotoxin at selected optimal concentration were detected by Western blot.Indirect immunofluorescence assay was used to measure the activation of p50,IκBα,and importinα3.Results The change of TNF-α and Caspase-3 in the dose-effect course,compared with control group,was significant (P ＜0.05 ).p50 was increased in the nucleus ( P ＜ 0.05 ),and IκBα was decreased in the cytoplasm ( P ＜ 0.05 ).cSN50( 100 μmol/L) partially blocked nuclear translocation of p50 and its downstream factor( P ＜ 0.05 ).Conclusion NF-κB nuclear translocation may play an important role in acute alcoholic liver injury.cSN50 can effectively inhibit NF-κB activity and its downstream gene expression in HepG2 cells.

Objective To assess the efficacy and safety of thiazolidinediones combined with metformin in treatment of polycystic ovary syndrome.Methods Electronic database searching was performed on Medline,Cochrane Library,EMbase,EBSCO,ScienceDirect,OVID,Springer LINK,Wiley,Chinese biology and medicine(CBM),China National Knowledge Infrastructure(CNKI),and Chongqing VIP Database;meanwhile,unpubished literature,conference papers and dissertations were also searched manually.The data included those from the establishment to November 2009.Randomized or clinical controlled trials concerning the thiazolidinediones and metformin combination therapy for PCOS were selected and assessed for the methodological quality,and Meta-analysis was performed using statistical software Revman 5.0.Results Five randomized controlled trials met the inclusion criteria and were included.Compared to metformin group,thiazolidinediones combined with metformin significantly improved insulin resistance(P

[Summary]_ Severe hypertriglyceridemia is the third common cause of acute pancreatitis following after alcohol abuse and cholelithiasis. Moreover, it is also an important risk factor of cardiovascular events. However, the cases of severe hypertriglyceridemia caused by autoimmune disease were rare in clinical, which would bring the difficulty for diagnosis and treatment. A better understanding of the clinical characteristics, possible pathogenesis, and corresponding therapy of the disease would be helpful, which would reduce the risk of complications, and finally improve both the survival rate as well as quality of life of these patients.

The trigemino-cerebellar projections of the rats were studied by introducing HRP microelectrophoretically in to various deep icerebellar nuclei (dentate nucleus, ND; interpositus nucleus, anterior part, NIA; interpositus nucleus, posterior part, NIP; fastigial nucleus, NF). The results indicate that all nuclei of the trigeminal nerve give their projections to bilateral (mostly ipsilateral) deep cerebellar nuclei. Most of them come from the interpolar and oral subnuclei of the spinal nucleus of the trigeminal nerve. The caudal subnucleus of the spinal nucleus of the trigeminal nerve and the principal sensory nucleus of the trigeminal nerve (VP) take the second place. Least of all come from the mesencephalic nucleus (ME) and the motor nucleus (MO) of the trigeminal nerve. In addition, cells in the region ventrolateral to the motor nucleus (VMO) and in the root of the trigeminal nerve (VR) also project to deep cerebellar nuclei. Fibers coming from ME terminate mostly in NF and NIA. Fibers from the spinal nucleus of the trigeminal nerve and VP terminate more in NIP and ND. Fibers from MO terminate in NF, NIA and ND. Fibers from VMO and VR have the same termination as those from the sensory nuclear complex of the trigeminal nerve.

[Summary] In newly diagnosed diabetic patients, fasting plasma glucose, HbA1C , and plasma lipid profiles were measured to analyze the association between HbA1C and plasma lipid profiles. HbA1C might affect plasma lipid profiles in newly diagnosed diabetic patients. Higher HbA1C was associated with the worse plasma lipid profiles and more severe insulin resistance.

Eight rabbits were used in this study.The position of the phrenic nucleus in thespinal cord,the morphology of the phrenic motoneurones and position of the cellbodies of the sensory neurons of the phrenic nerve were determined by using themethod of HRP labelling through the centralcutting end of the left phrenic nerve atthe root of the neck.The results were as follows:1.The phrenic nucleus in the rabbit was located in C_3,C_4,and C_5 segments.Itis a longitudinal cell column lying between the ventromedial and the ventrolateralcolumns of the ventral horn of the spinal cord.2.Phrenic motoneurones differed in shape and size.Most of the cell bodies ofthe rabbit's phrenic motoneurones were round or oval in shape,ranging from 5 to45 ?m(mean 25 ?m)in diameter.3.The rabbit phrenic nerve arises from the ventral rami of the 3 rd,4 th and5 th cervical nerves,and the nucleus of this nerve does not extend beyond the 3 rd-5 th segments——the location of the nucleus corresponds with the segmental rootsfrom which the phrenic nerve arises.4.The cell bodies of the sensory neurones of the rabbit's phrenic nerve werelocated in the dorsal root ganglia of the third and fourth cervical nerves.Besides,50 rabbits were dissected,and the origin of their phrenic nerves werestudied.

To study the function of lipoprotein lipase (LPL)with Asn291 Ser and Lys312insC compound mutation in LPL gene knockout heterozygous (LPL~(+/-)) mice. The results showed that triglycerides, free fatty acids, blood glucose and weight of LPL~(+/-) mice were higher than those of c57 mice(P<0. 05). The expressions of LPL Mrna and LPL protein of LPL~(+/-) group were lower than those of c57 group(P<0.05). The injection of Asn291Ser +Lys312insC protein caused little change of the lipid mass and LPL activity,but the injection of normal LPL protein induced obvious decrease of lipid mass and increase of the LPL activity.

Objective To compare the differences in responsive ability and oxidative stress damage between type 2 diabetic rats and normal rats under acute sepsis associated with stress hyperglycemia.Methods GK rats with type 2 diabetes and Wistar rats were established critical ill models of sepsis by intraperitoneal injection of 5 mg/kg lipopolysaccharide(LPS).Before and after LPS injection,serum levels of proinflammatory cytokines tumor necrosis factor α (TNF-α),interleukin (IL)-1β3,and IL-6,and anti-inflammatory cytokine IL-10 were determined.Malondialdehyde (MDA) and total antioxidative capacity (T-AOC) levels in serum and tissues (lung,liver,kidney,heart) were measured by colorimetric determination.Results Before LPS injection,serum levels of TNF-α,IL-6,and IL-10 in GK rats were higher than those of Wistar rats.The serum levels of TNF-α,IL-6,and IL-10 after LPS injection were raised in both GK rats and Wistar rats (all P＜0.05).At baseline,serum MDA level of GK rats was higher than that of Wistar group [(25.76 vs 12.71) μmol/L,P＜0.05],while T-AOC level were lower [(0.60 vs 2.8) U/ml,P＜0.05].One hour after LPS injection,the serum MDA level in two kinds of rats significantly increased compared with those of their baseline levels [(32.30 and 20.19) μmol/L,both P＜0.05],while their serum T-AOC level decreased [(0.20 and 2.08) U/ml,P＜0.05]).There were no signigicant differences in the change trend of serum MDA and T-AOC between the two kinds of rats.MDA and T-AOC levels in tissues had no significant difference before and after LPS injection in GK and Wistar rats (P＞0.05).Conclusions The rats with type 2 diabetes had lowered level of proinflammatory factors and raised level of anti-inflammatory factors under acute sepsis,presenting better tolerance and lowered probability of inflammatory diffusion compared with normal rats.

The trigemino-cerebellar projections of rats were studied by introducing HRP microelectrophoretically into various areas of the cerebellar cortex. The results indicate that the following parts of the cerebellum receive bilateral (mostly ipsilateral) trigeminal projections, namely, the simple lobule, the crusa Ⅰ and Ⅱ, the paramedian lobuIe, the dorsal paraflocculus, the lateral part of the lobule Ⅷ and the vermal cortex of the lobules Ⅵ～Ⅸ.Fibers from the interpolar subnucleus and the principal sensory nucleus of the trigeminal nerve project to all of the above mentioned areas.The caudal subnucleus projects to the crus Ⅰ, the paramedian lobule, the dorsal paraflocculus, the lateral part of the lobule Ⅷ and the vermal cortex of the lobules Ⅵ～Ⅸ.The oral subnucleus gives its projections to the crus Ⅱ, the paramedian lobule, the lateral part of the lobule Ⅷ and the vermal cortex of the lobules Ⅶ～Ⅸ.The mesencephalic nucleus of the trigeminal nerve sends fibers to the crura Ⅰ and Ⅱ, the paramedian lobule, the lateral part of the lobule Ⅷ and the vermal cortex of lobules Ⅶ～Ⅸ.A few labeled neurons were found in the motor nucleus of the trigeminal nerve; while in the region ventro-lateral to the motor nucleus, in the root of the trigeminal nerve and in areas adjacent to it large amount of labeled cells were seen in all the cases studied.Unexpectedly, several labeled neurons were seen in a semilunar ganglion of the trigeminal nerve.