Objective To investigate the year rhythm of laryngeal cough.Methods To record the attack months,the syndromes of TCM and degrees of symptoms in detail and analyze the data by percentage and Ridit analysis.Results The periods of laryngeal cough are in spring(March to May) and in autumn(September to November).The syndromes of TCM such as wind violating lung are commonly coughed in spring and primarily mild degree.The syndromes of TCM such as fire hyperactivity are commonly coughed in autumn and primarily moderate degree.Conclusions There is significant year rhythm in laryngeal cough.To master the rhythm can help the diagnosis and treatment.

Objective To study the expression changes and significance of human telomerase reverse transcriptase (hTERT)-mRNA and mutant p53 protein in thyroid carcinoma tissues in the elderly.Methods The expression level of hTERT-mRNA was examined by in situ hybridization in 50 samples of thyroid carcinoma tissues from the elderly and 41 samples of thyroid carcinoma tissues from the non-elderly and 30 samples of thyroid benign lesions.The expression level of p53 was examined by immunohistochemistry in all subjects.Results The positive rate of hTERT-mRNA was 96.0％ (48/50),85.3％ (35/41) and 10.0％ (3/30) in thyroid carcinoma tissues from the elderly,the non-elderly and thyroid benign lesions respectively,and there were significant differences between the three groups (x2=73.61,P=0.000).The positive rate of p53 was 92.0％ (46/50),85.3％ (35/41) and 13.3％ (3/30) in thyroid carcinoma tissues from the elderly,the non-elderly and thyroid benign lesions respectively,and there were significant differences between the three groups (x2 =62.30,P =0.000).Conclusions The positive rates of hTERT-mRNA and p53 in thyroid carcinoma tissues are higher in the elderly than in the non-elderly,which can be used to evaluate the biological behavior and prognosis of thyroid carcinoma in the elderly,and they play the important roles for targeted therapy.

One hundred cases of vocal nodules and vocal polypswere treated with the Powder with an effective rate of36%.mitigated rate of 27%.the total effective ratebeing 63%.The effective rate was related to the TCMtyping of syndromes,types,sizes and colors of vocalpolyps and has nothing to do with the locations of thelesion.After 3 months of treatment,the abnormalhemorhe ologicaal changes in vocal noddules an sessilevocal polyps were markedly improved.

Objective To study the changes of expression of mucin1 (MUC1) and protooncogene proteins C-myc (C-myc) gene in elderly papillary thyroid carcinoma.Methods The expression levels of MUC1 and C-myc were examined by immunohistochemical methods in 58 sample of thyroid carcinoma,35 nodular goiter and in 30 normal thyroid tissue.Results The detective rate of MUC1 in 58 specimens of thyroid carcinoma was 77.6％ (45/58),while 90.0％ (9/10) in those with infiltration and 88.2 ％ (15/17) in those with metastasis.The detective rate of C-myc in 58 specimens of thyroid carcinoma was 81.0 ％ (47/58),and 100.0 ％ (17/17) in those with metastasis.Conclusions The differences in MUC1 or C myc expression and in thyroid carcinoma infiltration and lymph node metastasia between benign versus malignant thyroid tumor are statistically significant.

Objective To investigate expression levels of epithelial mucin 1 (MUC1) and epitbelial mucin15(MUC15) in elderly patients with papillary thyroid carcinoma and assess the role of MUC1 and MUC15 in the pathogenesis of thyroid papillary carcinoma.Methods Protein expression of MUC1 and MUC15 was detected by immunohistochemistry in 10 samples from normal thyroid tissue adjacent to thyroid adenoma,57 samples from papillary thyroid carcinoma (PTC),and 14 samples from PTC in neck lymph node metastasis.Results Expression rates of MUC1 in normal thyroid tissues,thyroid papillary carcinoma,and lymph node metastatic carcinoma were 40.0％,75.4％,64.3,respectively,and the rates for MUC15 were 0,73.7％,71.4％,respectively.The positive expression rate of MUC1 was higher in PTC tissues than in normal thyroid tissues (x2 =5.10,P=0.02) and,compared with normal thyroid tissues,the positive expression rate of MUC15 increased in PTC tissues and lymph node metastatic carcinoma (x2 =12.25 and 19.75,both P＜0.05)MUC15 protein expression was higher in micro-PTC (less than or equal to 1 cm in diameter) than in carcinoma larger than 1 cm in diameter (90.9％ vs.62.9,x2 =5.48,P=0.02).MUC15 expression was higher in PTC without lymph node metastasis than in PTC with lymph node metastasis (83.8％vs.55.0％,x2 =5.55,P=0.02).MUC1 expression was positively correlated with MUC15 expression in thyroid papillary carcinoma (r=0.35,P=0.01).Conclusions MUC1 and MUC15 may have synergistic effects in the initiation and progression of PTC.MUC15 may play a role in regulating tumorigenesis of thyroid papillary carcinoma in early stages and can potentially serve as a supplementary marker in the screening of micro-thyroid papillary carcinoma.