1.Etiological detective analysis in female urinary tract infection
Chinese Journal of Primary Medicine and Pharmacy 2008;15(4):576-577
Objective To discuss the disposition feature of pathogenic bacterium in female urinary tract infection(UTI) ,so as to elevate the level of clinical diagnosis and therapy. Methods To cultivate medistream urine,assess vaginal secretions in the aspects of bacteriology, mycoplasma, chlamydia, mycetes and parasite. All specimen collected from 129 female patients who chiefly complained irritation of urinary tract ,from January 2003 to December 2006. Results Pathogenic microorganisms that found in the 129 female patients with UTI are gram-negative bacteria( 53.49 % ), gram- positive bacteria ( 19.38 % ), mycoplasma ( 14.73 % ), mycetes (9.30 % ), chlamydia (4.65 % ),parasite(1.55% ). Among them, escherichia coli, klebsiella pneumoniae bacilli, enterococcus faecalis, staphylococcus aureus are common species. Sexually transmitted disease(27.91% ) include the infection of diplococcus gonorrhoeae,mycoplasma and ehoamydiae. Among them,non-gonococcal urethritis is common. Combined infection(17.38 % ). Infection combined with mycoplasma urealytium and other pathogen is the most, these patients are most young or middle aged. Conclusion Pathogen in female UTI is mostly gram-positive bacteria. STD and combined infection should be paid attention to by clinicians. We suggest female UTI patients take etiological test regularly,so as to elevate the level of clinical diagnosis and therapy.
2.Practice and explorations of building a medical group in asset restructuring
Chinese Journal of Hospital Administration 2015;31(1):14-17
Introduced in the paper are the background,mode and development course of Taizhou Enze Medical Center (Group),with special focus on the concrete measures of its group management,including the reconstruction of human resource,finance,property,information,professional skills and knowledge resources.It also made recommendations based on its practice and experience.
3.Accelerated pace of clinical paths to optimize key medical service processes
Haixiao CHEN ; Jianghong ZHU ; Yaohui WANG
Chinese Journal of Hospital Administration 2010;26(7):492-495
The key to clinical paths administration lies in the optimization of the key processes and sectors in medical service, which will achieve an integration that is based on patients and span departments to cover all medical service sectors and processes. The clinical path practice was introduced into the hospital in 2005, and the clinical paths have been put into practice for over 20,000 cases/occasions by the end of 2009, scoring satisfactory social and economic outcomes as of now. For the purposes of speeding up the clinical path practice for expected outcomes in optimizing key medical service processes, we have got the following eight experiences and lessons: (1) Build shared demands; (2)Establish a powerful leadership; (3) Develop and promote visions; (4) Eliminate resistance and pool the forces; (5) Keep on the efforts and focus on details; (6) Reward involvements; (7) Recruit and develop key operators within the hospital; (8) Systemize and supervise the experiences of implementation.Promotion of clinical paths is a renovation in management with its inherent rules. Adoption of appropriate technical strategies and cultural strategies will upgrade the quality and acceptance of clinical path implementation.
4.Determining of Cyanogen Chloride in Drinking Water
Xiulan BIAN ; Yaohui CHEN ; Xiaochuan LIU
Journal of Environment and Health 1992;0(02):-
Objective To develop a method for determining cyanogen chloride (CNCl) in drinking water. Methods Pyridine-barbituric acid spectrophotometry was employed and some parameters including the pH value of the phosphate buffer, the time of color reaction, temperature, the concentration of chloramine T, the proportion of the phosphate buffer, the proportion of color agent and the maximum absorption wavelength were optimized. The linear range, limit of detection, precision and recovery rate were studied. Results Trace quantity of CNCl obeyed the Law of Beer. In the range of 0-0.1 ?g/ml CN-, the correlation coefficient was 0.999 4, the regression equation was y=0.194x-0.033, the limit of detection was 0.003 ?g/L, the lower limit of detection was 0.01 mg/L, the relative standard deviations were 1.5%-6.2% and the recovery rates were 92.9%-107.1%. Conclusion The method has the characters of accurate, sensitive, simple and rapid, it can be used in monitoring of drinking water quality.
5.Effects of ping-gan qianyang therapy on adrenal gland proteome in migraine rat model with hyperactivity of Hver-yang
Jianjun HU ; Zeqi CHEN ; Guangwei ZHONG ; Wei LI ; Yaohui YIN
Journal of Chinese Physician 2008;10(5):590-593
Objective To explore the molecule mechanisms of ping-gan qianyang therapy on migraine rat model with the hyperactivity of liver-yang.Methods An animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation and administering orally with Fuzi decoction.The total proteins of adrenal#and in the rats were separated by immobilized PH gradient-based two-dimensional gel electrophoresis, and the 2-DE images Was analyzed by PDQuest 7.0 software.Matrix-Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry(MALDI-TOF-MS)and SWISS-PORT and MSDB database were used to identify differential proteins.Result The well-resolution and reproducible 2-DE patterns of rat adrenal glands in normal group,model group and therapy group were obtained.A total of 8 differentially expressed proteins were identified.Conclusion Ping-gan qianyang therapy may effect on migraine by regulating the expression of proteins related to energy metabolism and ubiquitin.
6.Giant benign symmetric lipomatosis of the neck: four case reports.
Rong ZHU ; Xinchun JIAN ; Yaohui CHEN ; Xieshan HUANG ; Deyu LIU
West China Journal of Stomatology 2015;33(4):439-443
Benign symmetric lipomatosis is a rare disease and may appear as a huge tumor in the neck. Four benign symmetric lipomatosis associated with gigantic painless mass or neck motion limitation were reported. Operative technique of one-time radical resection or stage resection was used to remove these tumors. One patient had a postoperative complication of incision effusion and infection, and three patients had no significant complication. At more than one-year follow-up, the motion and appearance of a patient's neck returned to normal, and no recurrence was observed. The etiology, clinical manifestations, diagnosis, and treatment of the disease were discussed.
Humans
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Lipomatosis, Multiple Symmetrical
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Neck
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Recurrence
7.Effects of Butylphthalide on Neurological Function and Prognosis of Elderly Patients with Hypertensive In-tracerebral Hemorrhage after Trepanation and Drainage Surgery
Yaohui JIA ; Huimin CHEN ; Xianli GUO ; Yongkai GAO ; Yongqing DAI
China Pharmacy 2017;28(26):3701-3704
OBJECTIVE:To investigate the effects of Butylphthalide and sodium chloride injection on neurological function and prognosis of elderly patients with hypertensive intracerebral hemorrhage(HICH)after trepanation and drainage surgery. METH-ODS:During Jan. 2015 to Jun. 2016,80 elderly HICH patients were selected from our hospital and then divided into control group and observation group according to random number table,with 40 cases in each group. Both group received trepanation and drain-age surgery. Control group was given routine treatment. Observation group was given Butylphthalide and sodium chloride injection 100 mL,ivgtt,bid,on the fifth day after surgery,on the basis of control group. Both groups received treatment for 14 d. Clinical efficacies of 2 groups were observed. CSS scores were compared between 2 groups before surgery and 28 d after operation;volume of encephaledema,serum levels of homocysteine(HCY)and substance P(SP)were compared between 2 groups before surgery and 14 d after operation. RESULTS:The total response rate of observation group was 87.5%,which was significantly higher than 67.5% of control group, with statistical significance (P<0.05). Before surgery, there was no statistical significance in CSS scores,volume of encephaledema or serum levels of HCY and SP between 2 groups(P>0.05). CSS scores 28 d after operation, SP levels 14 d after operation were significanthy increased,volume of encephaledema and serum levels of HCY in 2 groups were significantly decreased,and the observation group was significantly better than the control group,with statistical significance(P<0.05). CONCLUSIONS:Butylphthalide and sodium chloride injection can significantly improve clinical efficacy and hepatic func-tion damage,relieve postoperative encephaledema,reduce serum levels of HCY and increase SP levels in elderly HICH patients af-ter trepanation and drainage surgery.
8.Determination of Stilbene Glucoside in Zishen Ningshen Pills by HPLC and Preliminary Study on Its Influence Factors
Xiqiang ZHANG ; Rongfeng LIN ; Yaohui HE ; Jianping CHEN ; Xiaoqi HUANG ; Xuguang SHI ; Ziren SU
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To establish a method of HPLC assay for determining stilbene glucoside in Zishen Ningshen Pills(ZNP),and to study the influence factors on the content of stilbene glucoside in the process of preparation.Methods HPLC was used for the determination of stilbene glucoside in ZNP.Through simulation the process of preparation,the stilbene glucoside content in the intermediate products was determined by HPLC,and its retention rate and metastasis rate were also investigated.Results The resolution and the linearity of stilbene glucoside were fine,the average recoveries being 98 % ~ 102 %.The retention rate of stilbene glucoside in the drying powder was 60.3 %,lower than that in the original medicinal powder.Conclusion The quantitative method for determining the ingredients in ZNP is simple,feasible and reproducible,and is beneficial for quality control of ZNP.The drying process under normal pressure is the main influence factors of the decrease of stilbene glucoside content,and the decompression drying can be taken into account to take the place of the atmospheric drying.
9.Blockade of intrathoracic lymphatic flow significantly ameliorates intestinal bacterial translocation in rats with obstructive jaundice
Xinze ZHOU ; Qinshen MAO ; Yaohui NI ; Ruixin CHEN ; Xiu YU ; Liping LU
Chinese Journal of General Surgery 1997;0(04):-
Objective To investigate the effect of lymphatic flow impedement on intestinal bacterial translocation in rats with obstructive jaundice. Methods Murine obstructive jaundice model was established by ligating lower choledochus. Sixty rats were divided into three groups: sham-operative group (group A), obstructive jaundice group (group B), and obstructive jaundice with thoracic duct ligation group (group C). At post-op day 15 vena cava inferior blood? portal vein blood were drawn, and endotoxin, TNF-?, IL-6 were determined. Baterial culture of mesenteric lymph nodes and lung were measured with aseptic manipulation. The extreme intestinal lumen mucosa and lung were studied pathologically. Results Blood concentration of endotoxin increased in group B and C ( P
10.Clinical Results of Surgical Treatment for Lumbar Spinal Canal Stenosis
Cuoping CHEN ; Yucai FEN ; Yuqiang GU ; Wenhu ZHU ; Ronghao CHEN ; Qiuhua GU ; Xiaoxiang ZHOU ; Yongsheng SONG ; Yaohui HUN
Journal of Medical Research 2009;38(8):66-68
Objective To investigate the surgical outcome of lumbar spinal canal stenosis. Methods Forward analysis of 160 cases of the patients with lumbar spinal canal stenosis getting operative treament was performed. 87 cases were male and 73 case were female. The average age was 51 years old (18 ~ 78years old). The average course of deseases was 5 years (1 month ~ 36years). All of the cases used lumbar spinal canal decompression combined with pedicle screws fixation and posterolateral bone graft. All cases had a follow - up of 3 months to 5 years (mean 34 months). Results The (COA) recovery rate among the tolal patients was cassified as exellent in 120 ca-ses , good in 31 cases , fair in 7 cases. The excellent and good rate was 94.4%. Conclusion The operative intervention was an effective method for patients with severe or progressive clinical lumbar spinal canal stenosis. The procedure in decompressed compretely through pos-terior approach and the instability of cerrical apinein had the satisfactory clinical outcome.