Objective:To study the clinical efficacy of botulinum toxin type A injection in the treatment of upper facial dynamic wrinkles.Methods:A total of 35 patients with upper facial dynamic wrinkles treated in our hospital from February 2016 to October 2016 were selected as research objects.All the patients were treated with botulinum toxin type A.The excellence rate of 30min after treatment,3 d after treatment and 7 d after treatment were evaluated and compared.The facial wrinkles severity index of the patients were evaluated by the facial wrinkles scale (FWS),and the differences of the FWS index before treatment,4 weeks after treatment and 6 months after treatment were compared.The adverse reactions,like upper facial tension,fatigue and local injection of ecchymosis after treatment were observed and compared.Results:The excellence rate of the patients after treatment of 30 min,3 d and 7 d were gradually increased.The excellence rate of the patients after treatment of 3 d and 7 d were significantly higher than that of after treatment of 30 min,and the excellence rate of the patients after treatment of 7 d were significantly higher than that of after treatment of 3 d (P＜0.05).The FWS index of the patients after treatment of 4 weeks and 6 months were significantly lower than those of before treatment,and the FWS index of the patients after treatment of 4 weeks were significantly lower than that of 6 months after treatment(P＜0.05).After treatment,only 4 patients had slight discomfort and all of these symptoms were disappeared after 3-4 days,there was no effect on their normal work and life.Conclusion:Botulinum toxin type A injection has a remarkable clinical curative effect in the treatment of patients with upper facial dynamic wrinkles,can maintain tor a long time,and also has good safety,it is worthy clinical application.

Objective:To investigate the curative effect of botulinum toxin type A combined with plastic operation of pouches on patients with eye skin relaxation and its impact on patients' satisfaction,and to provide reference for clinical application.Methods:A total of 60 patients with eye skin relaxation,who were treated in Baoji Central Hospital from June 2015 to June 2016,were selected as subjects and divided into control group (n=28) and treatment group (n=32) according to the patients' choices.The patients of control group were treated with plastic operation of pouches,while the patients of treatment group were treated with botulinum toxin type A combined with plastic operation of pouches.The two groups were followed up for 6 months.The improvement of crow's feet,the relief of pouches and the improvement of eye skin gloss of the patients after operation were observed and recorded.The incidence of complications were also observed.At the end of the follow-up,the satisfaction degrees of the patients were investigated with the self-made questionnaire.Results:After treatment,the improvement rate(100％) of crow's feet of the treatment group was significantly higher than that(60.71％) of the control group;the improvement rate (96.88％) of pouches of the treatment group was significantly higher than that (32.14％) of the control group;the improvement rate (87.50％) of eye skin gloss of the treatment group was significantly higher than that (53.57％) of the control group,and the differences were statistically significant (all P＜0.05).After treatment,there was no significant difference in the incidence of incision swelling,small amount of secretions and small hematoma between the two groups (P＞0.05),the operation eye swelling and incision bruises outer canthus in the control group were more than those in the treatment group (P＜0.05).The total satisfaction rate [93.75％ (30/32)] of the treatment group was significantly higher than that[67.86％ (19/28)] of the control group,the difference was statistically significant (P＜0.05).Conclusion:Botulinum toxin type A combined with plastic operation of pouches in the treatment of eye skin relaxation has a better efficacy and safety,which can significantly reduce the incidence of operation eye swelling and incision bruises outer canthus,and improve patients' satisfaction rate.It is worthy of clinical application.

Objective To investigate the prevalence of deep venous thrombosis (DVT) in burn patients,and to explore its influencing factors.Methods Clinical data of 2 506 burn patients admitted to our ward from January 2009 to January 2014,conforming to the study criteria,were retrospectively analyzed.Patients were divided into DVT group (n =26) and non-DVT group (n =2 480) according to whether or not DVT occurred during hospitalization.The incidence of DVT was calculated.The diagnosis time and type of DVT were recorded.The data of gender,age,depth of burn,total burn area,location of injury,cause of injury,infection of wound,venous transfusion of fluid (hypertonic solution and blood),location of intravenous catheterization,skin grafting,timing of first skin grafting after injury,D-dimer,bedridden duration after injury among patients between two groups were compared with chi-square test and Wilcoxon test.Indexes with statistically significant differences between two groups were selected,and they were processed with multivariate logistic stepwise regression analysis to screen the independent risk factors of DVT.Results (1) The incidence of DVT was 1.04％ (26/2 506).The diagnosis time of DVT was 16-62(40 ± 12)d,and patients diagnosed as having DVT after the 20th day post injury accounted for 92.3％ (24/26).All DVT occurred in lower limbs,with 1 case of central type,24 cases of peripheral type,and 1 case of mixed type.(2) There were no statistically significant differences in gender,location of injury (upper limbs,trunk,head and face),cause of injury,jugular vein catheterization,skin grafting,and timing of first skin grafting after injury among patients between two groups (withx 2 values from 1.853 to 3.742,Z =3.342,P values above 0.05).There were statistically significant differences in age,depth of burn,total burn area,burn in lower limbs,infection of wound,venous transfusion of hypertonic solution and blood,femoral vein and subclavian vein catheterization,D-dimer,and bedridden duration after injury among patients between two groups (with x2 values from 4.569 to 11.324,Zvalues respectively 7.357 and 7.012,P ＜0.05 orP ＜0.01).(3) Age,total burn area,burn in lower limbs,infection of wound,and D-dimer were the independent risk factors of DVT (with odds ratio respectively 2.904,2.655,3.574,2.786,3.142,95％ confidence interval respectively 1.504-7.652,1.368-6.594,1.958-8.511,1.459-7.001,1.922-8.062,P values below 0.05).Conclusions The incidence of DVT in burn patients is relatively low;it is diagnosed after the 20th day post injury in most patients,and the overwhelming majority is the peripheral type.Age,total burn area,burn in lower limbs,infection of wound,and D-dimer are the independent risk factors of DVT in burn patients,with which its occurrence could be predicted.

Objective: To investigate the changes of the intestinal mucosa-associated microbiota in the patients with ulcerative colitis (UC), and to explore their correlation with the clinical manifestations. Methods: From June to October 2016, at Gastrointestinal Endoscopy Center, the Second Affiliated Hospital of Xi′an Jiaotong University, 28 patients with UC and 16 healthy individuals who underwent colonoscopy examination were enrolled. The mucosa specimens of them were collected for fluorescent in situ hybridization (FISH). The bacterial flora were observed and counted, the correlation between the bacterial flora and the clinical manifestations were analyzed. Kruskal-Wallis test and Spearman rank correlation analysis were performed for statistical analysis. Results: Among 28 patients with UC, 16 were at active phase and 12 at remission phase. The number of total bacteria flora, Escherichia coli, Clostridium and Bacteroides of the active UC group and remission UC group were all more than those of healthy control group; however the number of Lactobacillus and Bifidobacteria were less than those of healthy control group, and the differences were statistically significant (χ²=23.34, 19.94; 23.40, 12.96; 23.39, 19.16; 23.32, 10.46; 23.19, 4.25; 18.94, 12.33; all P<0.05). The number of total bacteria flora, Escherichia coli and Bacteroides of the active UC group were more than those of the remission UC group, however the number of Lactobacillus and Bifidobacteria were less than those of the remission UC group, and the differences were statistically significant (χ²=7.32, 5.63, 5.62, 20.38 and 4.82; all P<0.05). In the patients with UC, the defecation frequency was positively correlated with the count of Bacteroides (r=0.459, P=0.014) and was negatively correlated with the count of Lactobacillus (r=-0.634, P<0.01). In UC patients, bloody stool, endoscopic appearance and total Mayo score were positively correlated with the counts of universal bacteria, Escherichia coli and Bacteroides (r=0.469, 0.403, 0.376; 0.604, 0.562, 0.475; 0.551, 0.463, 0.461; all P <0.05); and which were negatively correlated with Lactobacillus and Bifidobacteria (r=-0.570, -0.413; -0.899, -0.458; -0.862, -0.480; all P<0.05). The evaluation by the doctors was positively correlated with the counts of Escherichia coli (r=0.415, P=0.028), however was negatively correlated with Lactobacillus and Bifidobacteri (r=-0.841, -0.529; both P<0.01). Conclusion The intestinal microbiota have sigificantly changed in patients with UC which are correlated with some clinical manifestations of UC.