Cecropin is a kind of heat-durable and broad-spectrum antibacterial polypeptides which has strong effect against bacteria,fungi,virus and some pathogenic microorganisms.Today Cecropin has been widely applied into plant genetic engineering,antiviral study,and inhibiting tumor cell proliferation.Its Structure-function relationship,antibacterial mechanism,and the application on transgenic plants for bacterium resistance was reviewed.Expression of Cecropin in plants has a great application potential in bacterium resistance.Deep analyses and research of molecular structure and action mechanism can promote the transgenic plants antibacterial research.

Objective To evaluate the efficacy and safety of brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds for elderly non-small cell lung cancer.Methods 18 patients with peripheral lung cancer were undergone brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds.The effect was evaluated by periodically chest CT.The complications were analyzed.Results 18 patients were observed one,two and six months after operation.CR,PR,SD were seen in 27.8％(3/18),66.7％(14/18),5.6％(1/18)respectively of the patients.Pneumothorax,hemoptysis were seen in 16.67％(3/18),95.37％(17/18),no pleural cavity bleeding.Conclusion Brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds for lung cancer was effecitve.The complications were easy to treat.The methods of implantation of iodine-125 seeds could fully play the advantages of CT,and reduce the damage to the body when raising the tumorous control rate.

Objective To compare the effect of acupuncture and swallowing training on dysphagia in recove-ring stroke patients. Methods One hundred and thirty-two subacute stroke inpatients,73 males and 59 females,with a mean age of(67.9±9.0)years,26 cerebral hemorrhage patients and 106 cerebral infarct patients,participa-ted in the study.The patients were divided into a swallowing training group(ST,n=53)treated with thermal-tactile stimulation,deglutition exercises and food swallowing training;an acupuncture group(AP,n=48) treated with acu-puncture at the Fengchi(GB20),Lianquan(RN23),Bailao(EX-HN15),Jinjing(EX-HN12) and Yuye (EX-HN13)acupoints;and a control group(CG,n=31).The interventions were administered once a day,6 times a week.Before and after treatment,the patients were evaluated with a water-swallowing test and the"Any Two"test,and the arterial oxygen saturation(SpO_2) was monitored by pulse oximetry. Results The ST and AP groups showed significant improvement in water-swallowing and on the"Any Two"test after the treatment regimen,and were significantly better than the control group.There was no significant difference between the ST and AP groups.The average decrease in SpO_2 during water-swallowing was under 2%.After treatment,the baseline SpO_2 and post-water-swallowing SpO_2 of the ST group were significantly higher than those of the control group.The post-water-swallowing SpO_2 of the AP group was also significantly higher than that of the control group. Conclusions Acupuncture and swallowing training have similar effects in treating dysphagic stroke patients.Swallowing function and misaspiration cannot be predicted based on decreases in SpO_2.

Objective To study the relationship of fatty acid binding protein-2(FABP2)and apolipoprotein E(ApoE)with coronary heart disease(CHD)in type-2 diabetes mellitus(T2DM)patients.Methods FABP2 and ApoE gene polymorphisms were detected by PCR-RFLP.At the same time all CHD in T2DM patients' serum triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol were(HDLC),low-density lipoprotein cholesterol(LDLC)were detected.Results The T-allele frequencies of FABP2 were higher(P

OBJECTIVE To study the distribution and drug resistance conditions of inpatients in our hospital and make us antibiotics use reasonable.METHODS Retrospective analysis was conducted from all the pathogens of the clinical specimens from 2007-2008 and the data were analysed.RESULTS A total of 5508 pathogens were isolated from 2007-2008.Among 2441 pathogens(44.32%),there were Gram-negative bacilli(Enterobacteriaceae 1715 pathogens,Nonfermenters 707 pathogens,Vibrionaceae 19 pathogens),1154 pathogens(20.95%) were Gram-positive bacilli,1906 pathogens(34.60%) were Fungi.There were 331 pathogens which extended spectrum ?-lactamases(ESBLs) of Enterobacteriaceae(19.30%).The resistance of Escherichia coli to amikacin,ceftazidime,gentamicin,ciprofloxacin,piperacillin resistance rates were 10.94%,60.53%,62.08%,82.01%,86.46%,resistance of Enterobacter cloacae to amikacin,ciprofloxacin,gentamicin,piperacillin,ceftazidime resistance rates were 30.61%,46.29%,52.96%,55.20%,53.05%,resistance of Klebsiella pneumoniae to ciprofloxacin,amikacin,gentamicin,ceftazidime,piperacillin resistance rates were 13.04%,15.90%,16.90%,20.17%,30.07%,The resistance of E.coli,E.coli to ceftazidime,gentamicin,ciprofloxacin,piperacillin were not found were than 60.00%,E.cloacae to gentamicin,piperacillin,ceftazidime not more than 50.00%,K.pneumoniae to ciprofloxacin,amikacin,gentamicin,ceftazidime resistance rates more than 30.00%,not found E.coli,E.cloacae,K.pneumoniae PEI-pei South-resistant strains.The resistance of Pseudomonas aeruginosa to imipenem,amikacin,ciprofloxacin were less than 20.00%,the resistance rate of Acinetobacter baumannii more than 40.00%;of 199 methicillin resistant staphylococcus were not found out vancomycin-resistant S.aureus strains.CONCLUSIONS More of the pathogens show multiple drug resistance.It is urgent for surveillance of bacterial resistance and rational use of anti-microbial agents during the clinical therapy.

OBJECTIVE To know the status of management knowledge and disposal process of medical waste in village.METHODS Questionnaire was conducted among crowds randomly selected from Huishi,taipin,Gangou and Hepan town in Huining country of GanSu province.RESULTS The understanding rate of management regulation of medical waste among crowd in these four town was 12.31%;The touching opportunity to medical waste was very high;When medical waste was manipulated,people of chooseing to bury accounted for 12.15%,choosing to throw 43.35%,choosing localized manipulate accounted for 4.43%;The phenomenon of throwing medical waste was very serious(85.77%).CONCLUSIONS The understanding rate of management regulation of medical waste are not high,wehereas the touching opportunity was very high in the village.At the same time,the phenomenon of throwing medical waste was very serious and correct manipulating rate are not high.So we should strengthen the education of correlated policy,formulate eligible manipulating method,improve and consummate scientific management system of the medical waste in village.

Objective To measure and analyze the radioactivity level of 210Pb in outdoor air in Beijing in spring.Methods Portable high flow air samplers were used to collect outdoor air at the ground level to analyze the 210Pb radioactivity in the aerosol filter samples using a laboratory-based high purity germanium (HPGe) γ spectrometer.Results The activity concentration of 210Pb outdoors ranged from 267.2 to 1 697.6 μBq/m3,with an average of (878.7 ± 386.7) μBq/m3.Statistical analysis showed that the activity concentrations 210Pb of outdoors varied with variable air quality.Conclusions The activity concentrations of 210Pb outdoors are detectable in Beijing,varying considerably but within the normal range.

By applying the satellite-based distance rescue system and telemedical technology to the emergency treatment of the wounded from the Wenchuan Earthquake,we successfully conducted joint telemedical consultations with the guidance of the top specialists from various distant areas and saved 3 critically wounded patients.Our experience has shown that telemedical consultations may give a full play to the functions of large rescue organizations and the abilities of the top specialists,improve the treatment and expand war-time medical support.

Objective To construct and evaluate a nomogram for predicting the risk of peri-operative complications after radical retro-pubic prostatectomy (RRP) with standard classification criteria.Methods The peri-operative complications and clinicopathological data of 240 patients (50-82 years old) who had undergone RRP for prostate cancer from June 2006 to June 2011 were retrospectively collected.Gleason score:95 cases ＜ 7; 145 patients ≥ 7;Clinical staging:cT1 is 1 case (0.4％),T2a 5 cases (2.1％),T2b 7 cases (2.9％),T2c 162 cases (67.5％),T3a 26 cases (10.8％),T3b 39 cases (16.3％).The peri-operative complications (in 30 days after surgery) were classified by Clavien-Dindo Classification system (the occurrence rates are as follows:rectum injury 1.6％,wound infection 2.0％,deep venous thrombosis 1.2％,urinary leak 5.0％,lymphocele 4.5％,myocardial infarction 5.8％,second look operation 1.6％),and a logistic regression model was used to construct the nomogram.Results BMI,N staging and Blood loss more than 200ml during surgery were independent prognostic factors of RRP morbidity in multivariate logistic regression.The nomogram predicting the risk of peri-operative complications showed relative good concordance index (0.633) and good calibration. Conclusions Based on the clinicopathological factors,a nomogram to predict the probability of peri-operative complications in patients undergone RRP was constructed.This statistical tool may be beneficial in judging operation risk and help consulting with patient before or after surgery.

To explore the influence of matrix materials in complicate ingredients on traditional Chinese medicine and investigate the excipients selection model based on balanced release characteristics of multicomponents, the influence of HPMC (K4M, K15M, K100M) and Carbomer (934P, 971P, 974P) was illustrated by testing in vitro release of ginsenoside-Rg1, ginsenoside-Rb1 and notoginsenoside-R1 in Panax notoginseng saponins (model drug, PNS). According to in vitro release results of PNS matrix tablets in water and artificial intestinal juice, the release curves were analyzed with Peppas equation and simulating factor (f). Significant differences in k value and n value among ginsenoside-Rg1, ginsenoside-Rb1 and notoginsenoside-R1 existed in various formulations. The release behaviors from various excipients could be described with Non-Fickian transport or super Case II transport pattern. The f2 values for ginsenoside-Rg1, ginsenoside-Rb1 and notoginsenoside-R1 in 971P matrix tablet containing 30% Carbomer 971P were 74.91, 53.45, 57.89 in water and 79.35, 55.51, 51.89 in artificial intestinal juice, respectively. The release profiles fit for the regulation of FDA. The result revealed that the balanced release rates of Rg1, Rb1 and R1 in 971P matrix tablet were obtained.