Pseudomonas fluorescens is one of the most common contaminative bacteria of the stored blood. Ps. fluorescens No. 10-36 was cultured at 4℃, 22℃ and 37℃ for studying its growth phase and logarithmic growth phase. The results showed that this strain grew slowly at 37℃ with a generative rate of 900 minutes, but had no difference between the lag phase and the logarithmic growth phase. At 22℃ the lag phase were 5 hours, and the logarithmic growth phase were 29minutes, while at 4℃, the lag phase was 30 hours and the logarithmic growth phase was 300 minutes.

This study was aimed to analyze the influence of specific clinical effect of Dioscorea opposita Thunb. by dosage in order to summarize its rules. Recordings on pills containing D. opposita Thunb. from the classic prescrip-tion of the Formula Dictionary of Traditional Chinese Medicine were collected. Dosage of D. opposita Thunb. was used as the cardinal line. EXcellwas used to conduct schematic analysis. The results showed that small amount of D. opposita Thunb. had the effect of reinforcing the spleen. The effect of tonifying the lung had the dosage boundary line of 10 g. The small dosage had weak effect and large dosage had strong effect. The effect of tonifying the kidney was often the middle dosage. When the dose was larger or smaller, the effect was not obvious. The effect of consoli-dating the kidney was obvious when using larger or smaller dosage. It was concluded that the dosage of D. opposita Thunb. had relatively big influence on its effect, which provided reference of more value for the clinical practice and health cultivation.

Objective To evaluate the value of ultrasonic mini probe(UMP) in the diagnosis of lower digestive tract lesions . Methods one hundred and fifteen patients with lesions of lower digestive tract were undergone EUS with UMP (Olympus UM-3R, 20MHz; UM-2R, 12MHz). Results Eighty one cases with colorectal carcinoma appeared as a hypoechoic mass under UMP. UMP had an overall accuracy rate of 82. 7% (67/81) in the diagnosis of depth of infiltrative colorectal carcinoma. The sensitivity and specificity of UMP in the diagnosis of lymph node metastasis were 55. 4% and 68. 8% respectively. All 15 cases with submucosal tumors were diagnosed correctly under EUS except one leiomyoma misdiagnosed as leiomyosarcoma and other 10 and 9 cases were ulcerative colitis and Crohn's diseases respectively. Conclusions UMP has a high accuracy in determining the invasive depth of colorectal carcinoma and submucosal tumors. The preoperative UMP may provide much information and influence on the choice of therapy for colorectal carcinoma and submucosal tumors.

Combined Modality Therapy ; Eczema ; diet therapy ; drug therapy ; Feeding Behavior ; Humans

ObjectivesTo explore the impact of high body mass index (BMI) on irritable bowel syndrome (IBS) in Chinese adolescents.MethodsThe students were randomly selected from middle school and high school. They completed the epidemiological questionnaire of IBS and had their height, body mass measured.ResultsIn total, 1173 students were recruited, including 700 middle school students and 473 high school students. The incidence of overweight/obesity was 30.52%. The incidence of overweight/obesity was not significantly different between boys (28.40%) and girls (32.65%) (P=0.114), and there was not significantly different between students from middle school (30.57%) and high school (30.44%) (P=0.963). Two hundreds and twenty-one students suffered from IBS and the incidence of IBS was 18.84% with no significant gender difference. The incidence of IBS was significantly higher in students from middle school (13.43%) than those from high school (26.85%) (P<0.001). The incidence of IBS was not statistically significant be-tween overweight/obese students (22.07%) and normal weight students (17.42%) (P=0.061). The distribution of IBS subtypes had no statistical significance between overweight/obese and normal weight students (χ2=0.91,P=0.823). ConclusionsOverweight/obesity and IBS were extremely prevalent in Chinese adolescents, but the correlation be-tween them remains to be confirmed.

This study was aimed to determinate the content of quercetin from different parts (such as flowers, fruits, stems, leaves and branches) of Penthorum chinense Pursh. from Sichuan province so as to offer reference for the comprehensivedeve lopment and utilization of P. chinense Pursh. The quantitative determination of quercetin from five groups of different parts of P. chinense Pursh. were carried out and put into comparison by the reversed-phase high performance liquid chromatography (RP-HPLC). The results showed that there were larger differences of content among different parts. The highest content of the medical parts were flowers and fruits, which was followed by the leaves, stems and branches. It was concluded that different parts (such as flowers, fruits, stems, leaves and branches) of P. chinense Pursh. from Sichuan province should be divided in the clinical and productive practice, which was to supply the scientific basis for enhancing the curative effect and reasonable exploitation and utilization of the resource of P. chinense Pursh.

Household questionnaire survey was conducted in 200 residents of Tilan and Zhanhqiao neighborhoods, Xingang community, Shanghai to know their awareness of knowledge about pulmonary tuberculosis (TB) prevention and treatment. Results showed that 87.0% of the residents surveyed knew TB symptoms, but only 42. 5% knew cough and expectoration were pulmonary TB symptoms, 76. 5 % knew that TB could not be transmitted by use something that was used by TB patients, 85.5 % knew government policy of free and payment reduction for TB treatment, and 82. 0% knew there existed some hospitals for TB treatment designated by local health administrations. There was significant difference in the awareness between the two neighborhoods ( P ＜ 0. 01 ). Factors related to their awareness of TB prevention and treatment knowledge included family annual income, educational level, publicity by telecision and broadcasting.

OCT angiography (OCTA) is a non-invasive imaging technique for detecting blood flow information of the retina and choroid.Dye injection is not needed with OCTA,which is different from fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA).OCTA is able to observe blood flow in different retinal and choroidal segmentation slab.This revolutionary breakthrough in OCTA algorithm provides more and more accurate blood flow informations in the diagnosis of ocular vessel diseases and the study on pathogenesis of some vessel-related eye diseases.However,like other biometric technology,OCTA has its limitations and shortcomings,for example,OCTA presents a smaller observational area than FFA and ICGA,and some factors affect the imaging quality and cause misdiagnosis during the examination and reading image.Fully understanding the principle of OCTA and its image features are helpful for eye doctors to better interpret the blood flow changes of retinal diseases,choroidal diseases,glaucoma and neuro-ophthalmic diseases.Ophthalmologists should correctly apply this imaging tool for a better monitoring and following up of these diseases.

The afferent connections to the nucleus amygdaloideus centralis and nucleusamygdaloideus medialis in 42 albino rats were examined by means of retrogradetransport method of horseradish peroxidase(HRP).The microinjection syringe needle or microelectrophoretic pipette was insertedstereotaxically from vertical or oblipue approaches.The injections of HRP weregiven in the central and medial nuclei of the amygdaloid complex.The results were as follows:Following the injections of HRP into the nucleus amygdaloideus centralis,label-ed cells were always found in prepiriform cortex,periamygdaloid cortex,entorhinalcortex,ventral part the caudoputamen and globus pallidus,nucleus lateralis hy-pothalami,nucleus parafascicularis,corporis geniculati medialis,nucleus raphe dosalis,nucleus parabrachialis,locus coeruleus and zona reticulate substantia nigra.While HRP was injected into the nucleus amygdaloideus medialis,the labeledcells were always found in bed nucleus of stria terminalis,nucleus ventromedialis(hypothalami)and substantia nigra pars compacta.As the injections were given from lateral oblique approaches,labeled cells werenot found in the neo-cortex.There were no labeled cells demonstrated in the nuc-leus solitarius.In the above-mentioned areas nucleus ventromedialis(hypothalami),nucleusparabrachialis and locus coeruleus were often labeled bilaterally while others,ipsila-terally.

This article reports preliminarily the effects on the antibacterial activity & cytotoxicity of the derivatives of two diterpenoid components, Amethystoidin A & Rabdosia macrophylla C(i.e.Orido-nin ) which were isolated from the medicinal plants of Rabdosia ( Bl. ) Hassk. The experiment demonstrated that diacetate derivative of Amethystoidin A ( n ) could significantly enhance the antibacterial effect being 2 - 3 times more potent than that of Amethystoidin A. Hydrolytic derivative of Rabdosia macrophylla C (V) had a significant increase in the cytotoxicity on heptoma cell (QGY-7703 ) in vitro.