Arsenic trioxide (As2O3)is the main component of traditional Chinese medicine arsenic,which was initially used in the treatment of blood system diseases.In recent years,studies have found that it also has a good effect on a variety of solid tumors,especially gastric cancer.It has been confirmed that As2O3 can inhibit proliferation and metastasis of gastric cancer cells,induce apoptosis and protective autophagy,inhibite lymphatic formation and angiogenesis,affect the expression of telomere-related proteins in gastric cancer cells.

Aim To examine the protective effects of allopregnanolone against seizure on different animal models.Methods　The protective effects of allopregnanolone against maximal electrical seizure (MES) and picrotoxin-induced seizure were studied in C57 mice 15 minutes after vehicle or drug intraperitoneal administration.Results　In the MES test, we found that pretreatment with the phenobarbital or allopregnanolone produced a dose-dependent protective effect against seizure. The potency (ED50 value) of phenobarbital in the MES test was 2.40 mg·kg-1, with 95% confidence interval range from 1.22 to 4.72 mg·kg-1. The potency (ED50 value) of allopregnanolone in the MES test was 0.086 mg·kg-1, with 95%confidence interval range from 0.037 to 0.201 mg·kg-1, which was significantly higher than that of phenobarbital (P<0.01). The combination study of half ED50 values of phenobarbital and allopregnanolone resulted in a 80% of protective effect in MES test, which was higher than 50% produced by either phenobarbital or allopregnanolone at their ED50 values respectively. This result indicated that there was a synergism between phenobarbital and allopregnanolone in their anticonvulsant activities. In the picrotoxin test, we found that pretreatment with the allopregnanolone also produced a dose-dependent protective effect against picrotoxin-induced seizure. The potency of allopregnanolone in the picrotoxin seizure test was 0.123 mg·kg-1, with 95% confidence interval range from 0.058 to 0.263 mg·kg-1.Conclusion　Allopregnanolone(ip) could protect different seizures in a dose-dependent manner,had a higher potency than phenobarbital,and had synergism with phenobarbital in the MES test.

Objective To investigate the effects of hemo oxygenase-endogenous carbon monoxide (HO-CO) on mortality of septic shock in rats. Method Eighty healthy Sprague-Dawley rats were randomly divided into four groups (n=20) :group-C,group-Z, group-SS and group-LZ. MAP was monitored continuously,and deaths within 7 days were recorded. Evans blue in lung, ALT, AST, Cr and BUN in plasma, SOD and MDA in kidney, lung and liver,and HO-1 mRNA,HO-2 mRNA,HO-1 protein and HO-2 protein in kidney, lung, femoral artery and liver were measured.Data were analyzed usiny ANOVA. Results ① Mortality in group-SS was lower than that in group-LZ (P<0.05). ②MAP in group-LZ was greater than that in group-SS (P<0.05). ③ Plasma ALT, AST, Cr and BUN,and MDA contents of liver, kidney and lung, and Evans blue content of lung in group-SS were lower than those in group-LZ (P<0.05). Contrarily,the plasma levels of COHb and SOD activity in liver, kid-ney and lung in group-SS were greater than those in group-LZ (P<0.05). ④ HO-1 mRNA and HO-1 protein levels in liver, kidney, femoral artery and lung tissues in group-LZ were lower than those in group- SS (P<0.05). There were no significant difference in HO-2 rnRNA and HO-2 protein found between one another of four groups (P>0.05). Conclusions The up-regulation of HO-1 protein followed by increase CO can reduce the mortality during septic shock, while the hypotension, which is partly attributed to HO-1 protein and CO,matters little to mortality.

Objective To investigate the inhibitory effect of RNA interference(RNAi)on WT1 gene expression in HL-60 cell line.Methods Several pairs of small interfering RNA(siRNA)for WT1 gene was designed and transfected into HL-60 cells.WT1 gene expression was assessed by RT-PCR,and WT1 protein expression was tested by Western blot.Flow cytometry was used to measure the cell apoptosis.Results The expression rates of WT1 mRNA and the expression rates of WT1 protein were(23.62%?1.28%),(23.20%?1.04%),respectively in HL-60 WT1 siRNA-transfected cells,significantly lower than those of control cells(P

Objective To study the effect and the mechanism of baicalin on invasion and metastasis of(human) hepatocellular line BEL-7402.Methods Human hepatocellular line BEL-7402 were cultured and treated respectively with 5,10,20 mg/L baicalin for 48 h.The invasion and adhesion of the BEL-7402 cells were examined in Boyden chamber,cell migration and morphology were also observed.The expression of MMP_(2),TIMP_(2),_()integrin ?_(1,)and E-cd proteins of human hepatocellular line BEL-7402 was determined by flow cytometry.Results Baicalin inhibited invasion,adhesion and migration of hepatocellular line BEL-7402 in vitro in a dose dependent manner(P

The 2017 Annual Meeting of the American Society of Clinical Oncology (ASCO) was held on June 2-6,2017 in Chicago,United States.And the academic event showed the frontier researches in the field of oncology.Survival prediction in patients treated by FOLFIRI regimen and bevacizumab for metastatic colorectal cancer using radiomics approach was made in Abstract 3601.Authors aim to summarize the application and development of radiomics in digestive system tumors.

Objective To investigate the effects of minocycline hydrochloride ointment on collagenase in gingival crevicular fluid.Methods 60 patients with chronic periodontitis treated in our hospital from January 2015 to December 2016 were selected as the research object, the patients were divided into two groups according to their intervention methods, 30 cases in each group.The control group was treated with ultrasound clean governance and iodine glycerol, the observation group was treated with ultrasound clean governance and minocycline hydrochloride ointment The experimental data of the two groups were compared.Results The index change of gingival crevicular fluid volume plaque index,,depth of exploration and clinical attachment loss in the observation group were better than those in the control group, the difference between the two groups was statistically significant (P<0.05);The clinical therapeutic effect of the observation group(93.3%)was better than that of the control group(73.3%), and the difference between the two groups was statistically significant (P<0.05).The incidence of adverse reactions in the observed group(10.0%)was lower than that in the control group(26.7%), and the difference between the two groups was statistically significant (P<0.05).Conclusion Minocycline hydrochloride ointment can affect the level of collagenase in gingival crevicular fluid, and the clinical effect of the treatment of chronic periodontal disease is significant.

Medical English listening is an important part of English education in medical institutes. On the basis of schema theory,this paper tries to explore the application of the theory in the teaching practice of medical listening and focuses on how to improve the teaching quality of medical English listening from the perspectives of phonetic schema,vocabulary schema and background schema.

Objective To study the effect of minocycline hydrochloride ointment and ibuprofen on serum concentration of chronic periodontitis . Methods 86 patients with chronic periodontitis treated in our hospital from June 2014 to June 2016 were selected and randomly divided into two groups:observation group 43 cases, using minocycline hydrochloride ointment combined with ibuprofen treatment , the control group 43 cases, only the use of minocycline hydrochloride ointment for treatment.After treatment, the clinical efficacy of the two groups were observed, the level of plaque, the level of attachment, the level of gingival sulcus bleeding and the probing depth of periodontal pocket were recorded, the levels of IL-10, TNF-α, hs-CRP and IL-8 in serum and the incidence of adverse reactions were measured.Results The total effective rate was 90.7% in the observation group and 74.4%in the control group, which was significantly higher than that in the control group, the difference was statistically significant (P<0.05).The probing depth of the periodontal pocket, gingival sulcus bleeding index, attachment level and plaque index decreased to varying degrees in the two groups before and after treatment, the difference was statistically significant (P<0.05), patients in the observation group were significantly lower than those in the control group, the difference was statistically significant (P<0.05).After treatment, the levels of IL-8, hs-CRP and TNF-αin serum of the two groups decreased, IL-10 levels increased in different degrees, the difference was statistically significant (P<0.05).The observation group was better than the control group, the difference was statistically significant ( P<0.05 ) .Conclusion Minocycline hydrochloride ointment combined with ibuprofen has a good effect on patients with chronic periodontitis.It can effectively relieve the patient's condition and improve the serum concentration of patients.

AIM: To investigate the mechanism that antioxidants TA9901, inhibit the formation of amyloid-?-protein(A?) fibril. METHODS: Fourier-transform infrared spectroscopy was used to study the secondary structure changes on aging A? in vitro. RESULTS: A? aged alone for 30 min, the content of ?-pleated sheet and ?-turn were 43.17% and 32.9% respectively. A? aged alone for 7 days, the content of ?-pleated sheet increased abuot 10% and produced a shift of random coil toward ?-pleated sheet. TA9901 induced a significant decrease of the content of ?-turn (23.5%) and ?-pleated sheet (26.4%). VE mainly decreased the ?-pleated sheet content (30.8%). The combination of TA9901 and VE promoted transition of ?-turn (16.7%) toward ?-helix and random coil. CONCLUSIONS: Both of TA9901 and VE can effectively diminish the ?-structural content. TA9901 showed more intensitive inhibition than VE. The effect of TA9901 on the secondary structure of aged A? was associated with the mechanism that TA9901 inhibited A? aggregation and fibril formation.