Objective To get new more sensitive signs reflecting cartilage degeneration,which were added to the present ARA differential criteria of knee osteoarthritis and to compare the sensitivity and specificity before and after new items.Methods Through review analysis of the clinical feature,radiography and operative presentation of 115 adult patients,the new signs reflecting cartilage degeneration as items were found,which were added to the present ARA differential criteria of knee osteoarthritis as new criteria.Then the 112 patients who have received either knee joint replacement or arthroscopy were re diagnosed with both the present ARA criteria and the new criteria.Sensitivity and specificity were calculated and compared.Results "Extensive knee joint tenderness" with cartilage degradation and "lockage on motion or a positive Apler test" were significantly related to the meniscus degradation.A 39 7% sensitivity of these patients was obtained using the present ARA clinical criteria,while it reached 70 6% using the new clinical criteria ( P 0 05).Conclusion The two new items added to the present ARA criteria help to increase the sensitivity for diagnosis of knee osteoarthritis without influence on the specificity.

Separation of complex mixtures needs more resolving power. In two dimensional liquid chromatography (2D LC), the sample is subjected to two different separation conditions or mechanisms, which significantly increases the peak capacity,resolving power and reduces peak overlap. Meanwhile, 2D separation is more reliable for peak i-dentification from run to run than single-dimension chromatography, because two independent elution times are ob-tained for each peak. Among different 2D liquid chromatographic combinations/configurations, online comprehensive 2D-LC has appeared to be very attractive due to its high peak capacity, minimal sample loss, good reproducibility, and easy to automation, especially for separation of very complex samples. The present review discusses the theoreti-cal aspects and hardware configuration of online 2D-LC, particularly online comprehensive 2D-LC. It highlights its concept, features, advantages, applications and prospective in TCM quality Control analysis.

Objective To quantitatively evaluate the thickness of the buccal cortical bone in the posterior region of the maxillary for orthodontic micro‐implants in children .Methods Forty children (20 males and 20 females) in permanent dentition with Class Ⅱdivision 1 malocclusion were selected .The maxillary posterior alveolar bones of the subjects were scanned and reconstructed by cone‐beam computed tomography (CBCT) .The thickness of the cortical bone between the dental roots of the second upper premolar and the first upper molar at the height of 3 ,4 ,5 ,6 ,7 ,8 ,9 mm from the alveolar crest were determined .The data were analyzed sta‐tistically .Results The thickness of the cortical bone between the second upper premolar and the first upper molar was minimum at the height of 4 mm from the alveolar crest(P<0 .05) .The thickness of the cortical bone increased along with the distance increas‐ing from the alveolar crest at the height of 5 mm .The cortical bones in male were significantly thicker than those in female at the height of 7 ,8 ,9 mm (P<0 .05) .Conclusion The thickness of the buccal corticalbone in children in permanent dentition with ClassⅡ division 1 malocclusion changes with the measured vertical position .Micro‐implant anchorage is better to be placed in the area with a distance of 5 mm from the alveolar crest .

Objective To evaluate the clinical effect of levofloxacin combined with Jingangteng particle in the treatment of patients with chronic pelvic inflammatory disease.Methods According to the random number table, sixty -four patients with chronic pelvic inflammatory disease were divided into control group(n =32)which were treated with levofloxacin and treatment group(n =32)which were treated with levofloxacin and Jingangteng particle for three weeks.The clinical effect,symptoms and signs were evaluated and the adverse reactions between the two groups were observed as well as serum C reactive protein(CRP)were measured before and after treatment.Results The score of symptoms and signs of the treatment group were significantly lower than the control group,the difference was statistically significant [(2.61 ±2.09 )points vs.(5.32 ±3.28)points,t =3.941,P =0.000;(1.47 ± 1.02)points vs.(2.56 ±2.04)points,t =2.703,P =0.008].The level of serum CRP of the control group was (5.46 ± 1.93)mg/L,which was statistically higher than (3.76 ±1.06)mg/L of the treatment group after treatment,the differ-ence was statistically significant (t =4.367,P =0.000).The total effective rate of the treatment group was 96.9%, which of the control group was 78.1%,the difference between the two groups were statistically significant(χ2 =5.142,P =0.023).There was no significant adverse reactions during the treatment.Conclusion Levofloxacin com-bined with Jingangteng particle can obviously improve the symptoms and signs of chronic pelvic inflammatory disease, they have good safety and are worthy of clinical popularization and application.

Objective:To Compare root resorption between T loop technique and sliding technique of orthodontic treatment.Meth-ods:90 patients with Angle class I malocclusion were orthodontically treated by MBT fixed appliances with T loop technique(n =45) and sliding technique(n =45)respectively.Root resorption of the patients were examined by grading evaluation method with orthopan-tomography and periapical films before and after orthodontic treatment.Results:After orthodontic treatment the tooth root resorption rate increased(P <0.05),the root resorption rate was not significantly different between T loop technique group and sliding technique group(P >0.05).Root resorption mostly occurred in the incisors,especially in mandibular tooth.Conclusion:There is no difference of the effect of root resorption between T loop technique and sliding technique of orthodontic treatment in patients with Angle class I malocclusion.

BACKGROUND:In recent years, prolift mesh pelvic materials have been widely used in the pelvic floor reconstruction. Scholars have been exploring the advantages and disadvantages of the prolift mesh pelvic reconstruction and the traditional hysterectomy for pelvic floor reconstruction. ＜br> OBJECTIVE:To systemical y assess prolift mesh pelvic reconstruction versus traditional hysterectomy for pelvic floor reconstruction. ＜br> METHODS:Wanfang, VIP, PubMed, Medline databases were searched for randomized control trials (RCTs) related to the clinical effectiveness of prolift mesh pelvic reconstruction versus traditional hysterectomy for pelvic floor reconstruction published from 1996 to 2014. Meta analysis of acquired data was performed through the use of RevMan5.2 software. ＜br> RESULTS AND CONCLUSION:Nine RCTs involving 780 patients were included. Of the 780 patients, 398 received prolift mesh pelvic reconstruction and 382 underwent traditional hysterectomy. Compared with, prolift mesh pelvic reconstruction was superior to the traditional hysterectomy group in terms of operative time, intraoperative bleeding, hospitalization duration, evacuation time, postoperative body temperature, and cure rate of 18 months (P＜0.05). However, there were no significant differences in the post-void residual volume, recurrence rate, the cure rate of 6 months and 12 months, quality of sexual life after 1 year postoperatively (P>0.05). These results reveal that the prolift mesh pelvic reconstruction exhibits better short-term effects on the pelvic floor dysfunction, but its long-term effects need to be further verified.

Objective To study the correlation of expression of N-myc downstream regulated gene 1(NDRG1) with invasion of breast invasive duct carcinoma and lymph node metastasis. Methods A total of 71 specimens including 26 case of primary breast invasive duct carcinoma with lymphnode metastasis,45 case of nonmetastasis breast invasive duct carcinoma were observed.NDRG1 was detected by immunohistochemistry in formalin-fixed and paraffin-embedded sections.At the same time,the correlations of NDRG1 with E-cad,MMP2,MMP9,and TIMP2 were investigated. Results The expression of NDRG1 in breast cancer with metastasis of lymph nodes(9/26) was lower than that of non-metastasis of lymph nodes(32/45)(P

RESULTS: The drug carrier was modified with bifunctional-ligands, which could help the drug delivery system penetrate the blood-brain barrier and concentrate on lesions, thus improving the drug efficacy and reducing the side effects.

OBJECTIVE: To extract and isolate peristrophine from Peristrophe roxburghiana Brem and establish a content determination method of peristrophine. METHODS: Whole plant of Peristrophe roxburghiana Brem was extracted with 60% ethanol, then purified with D101 resin and preparative high-performance liquid chromatography (HPLC). High performance liquid chromatography (HPLC) using a mobile phase consisting of 55% methanol was used for the content determination (γ = 236 nm). RESULTS: The lineal range of peristrophine was 5.0 - 50.0 mg · L-1 (r = 0.9998), the mean recovery was 97.67%, and RSD was 0.66% (n = 9). CONCLUSION: This method can be used as the basis for the further research of Peristrophe roxburghiana Brem. Copyright 2012 by the Chinese Pharmaceutical Association.

Objective:To investigate the effect of different schemes of intrauterine insemination (IUI) on pregnancy outcome in patients with unexplained infertility.Methods:The clinical data of 393 cases (934 cycles) with unexplained infertility patients who underwent IUI from March 2016 to August 2018 in Dalian Maternal and Child Health Hospital were retrospectively analyzed. The patients were divided into natural cycle group (402 cycles) and promote ovulation cycle group (532 cycles). In promote ovulation cycle group, the patients were divided into clomiphene (CC) subgroup (124 cycles), letrozole (LE) subgroup (107 cycles), menotropins (HMG) subgroup (87 cycles), CC + HMG subgroup (103 cycles), LE + HMG subgroup (111 cycles). The pregnancy outcomes of each group were compared.Results:The clinical pregnancy rate and live birth rate in promote ovulation cycle group were significantly higher than those in natural cycle group: 14.10% (75/532) vs. 9.20% (37/402) and 11.28% (60/532) vs. 7.21% (29/402), and there were statistical differences ( P<0.05). There were no statistical differences in abortion rate, ectopic pregnancy rate and twin pregnancy rate between 2 groups ( P<0.05). Ovarian hyperstimulation syndrome occurred in 7 cycles, all of which were in CC + HMG subgroup. The clinical pregnancy rate and live birth rate in LE subgroup, HMG subgroup, CC + HMG subgroup and LE + HMG subgroup were significantly higher than those in CC subgroup: 11.21% (12/107), 13.79% (12/87), 16.50% (17/103), 21.62% (24/111) vs. 8.06% (10/124) and 7.48% (8/107), 11.49% (10/87), 13.59% (14/103) and 20.72% (23/111) vs. 4.03% (5/124), the abortion rate was significantly lower than that in CC subgroup: 3/12, 1/12, 2/17 and 1/24 vs. 5/10; the clinical pregnancy rate and live birth rate in HMG subgroup, CC + HMG subgroup and LE + HMG subgroup were significantly higher than those in LE subgroup, the abortion rate was significantly lower than that in LE subgroup, and there were statistical differences ( P<0.05). Conclusions:When the patients with unexplained infertility are assisted by IUI, LE combined with HMG ovulation induction can achieve a better pregnancy outcome.