Obesity and its related metabolic diseases become major health problems in the world.Adipose tissue plays an important role in the development of obesity.FSP27,a member of the CIDE family proteins,is expressed at high levels in white adipose tissue and differentiated 3T3L1 cells.The objective of current study is to establish a FSP27 knockdown preadipocyte cell line to investigate the in vivo function of mouse FSP27.The double strand siRNA of mouse FSP27 corresponding to nucleotides 270 to 291 was synthesized and inserted into pSilencer2.1.pSilencer-siFSP27 was co-transfected into 293T cells with the HA-mFSP27 expression vector to test its knock-down efficiency.The FSP27 siRNA was then transferred to a lentiviral vector.Lentivirus were generated and used to infect 3T3-L1 cells.It was shown here that lentivirus containing FSP27siRNA can effectively knockdown FSP27 expression in 3T3-L1 cells.Establishment of FSP27 knock-down cell line provides a useful tool for the study of in vivo function FSP27.

Objective To take the initiative in Huaibei region of Pseudomonas aeruginosa OprM efflux phenotype and the presence of genetic analysis to explore the multi-drug resistant Pseudomonas aeruginosa and the active efflux mechanism.Methods assignment pump inhibitor carbonyl cyano-right-chlorophenyl hydrazone(CCCP)on Pseudomonas aeruginosa ciprofloxacin(CIP) reversal of the sensitivity tests to screen active efflux of Pseudomonas aeruginosa phenotype-positive bacteria;using PCR amplified active efflux phenotype-positive bacteria OprM genes.Results CCCP under the action of 36 Pseudomonas aeruginosa,24 strains of the CIP to improve the sensitivity of four times more active efflux phenotype positive rate was 66.7%(24/36);in OprM gene PCR extension by experiment,there are 16(44.4%,16/36)was amplified 848bp fragment of OprM.Conclusion active efflux phenotype in clinical Pseudomonas aeruginosa in widespread;OprM of Pseudomonas aeruginosa genes in active efflux of the most common bacteria.

A hundred thirteen ears in 76 cases underwest the traumatic deafness caused by beating at the ear and head were examined with the Evoked Potential System of Nicolet Spirit and the Pure tone audiometry.113 ears were divided into two groups. One group consisted of 32 ears with the tympanal perforation, which were further divided into small, medium and big perforations. The second group consisted of 81 ears without tympanal perforation,which were classified into mild, moderate,serious,severe and profound deafness. The two groups were all examined by EcochG and ABR. The wave latent periods of EcochG and ABR of two groups (small, medium perforation;mild,moderate deafness)were statistically analysed and were compared with those of the normal control group. The results of the square deviation analysis and Q test showed the significant difference (76. 7%, P

Objective To investigate the effects of surgical method on the falx paraneoplastic parasagittal meningiomas.Methods 30 cases of surgical treatment in sagittal sinus and falx meningioma patients with imaging data,surgical approach,microsurgical resection of the tumor method and efficacy were analyzed.Results 30 patients resected by Simpson standard,Ⅰ grade resection 23 cases,Ⅱ grade resection in 7 cases,no operative mortality.1-5 years of follow-up,2 patients relapsed,all secondary surgical cure.Conclusion To be familiar with microscopic neuroanatomical relationship,using microsurgical resection of the superior sagittal sinus,falx meningioma,tumor removal rate can be increased to reduce the important functional areas of damage,reduce complications and improve quality of life of patients.

Objective To study the role of NF-?B pathway in HGF (hepatocyte growth factor)-mediated proliferation of WB F-344 cell. Methods WB F-344 cells were treated with HGF in different concentrations i.e. 0, 20, 40, 80 and 100ng/ml. After incubation for 24 hours, proliferation effect was analyzed with [3H] Thymidine. WB F-344 cells transfected with I?B? plasmid mutants by liposome transfection were used to block NF-?B activity, and then they were treated with 40ng/ml HGF for 0, 15, 30, 60 and 120min, respectively. The nucleoprotein was extracted to determine the NF-?B DNA-binding activity by EMSA. WB F-344 cells were also transiently transfected with BD Great EscA PeTM SEAP vector with liposome and then stimulated with HGF in different concentrations (0, 10, 20, 40ng/ml) for 8 hours to detect the NF-?B transcription activity. WB F-344 cells were pre-treated with NF-?B inhibitor BAY-11-7082 or ASA, and then with 40 ng/ml HGF for 24 hours to analyze the proliferation effect by [3H] Thymidine. Results HGF promoted the proliferation of WB F-344 cells and exhibited positive dose-effect tendency. HGF could enhance the NF-?B DNA-binding activity. NF-?B complexes appeared 15min after addition of HGF. The complexes reached the peak value 30-60min after addition of HGF and then decreased gradually. HGF could also enhance the NF-?B transcription activity, exhibiting positive dose-effect tendency. HGF-mediated proliferation was remarkably inhibited when NF-?B activity was blocked by BAY-11-7082 or ASA. In WB F-344 cells transfected with p-CMV-I?B?, the proliferative response to HGF was completely interrupted. Conclusions HGF may promote the proliferation of WB F-344 cells in a positive dose-effect tendency. The activation of NF-?B is necessary for HGF-mediated proliferation of WB F-344 cells.

Objective To explore how to establish and safeguard the peripherally inserted central catheters(PICC) maintenance network for the management and practical activity of PICC,so as to provide continuous high-quality PICC maintenance for discharged patients with PICC. Methods 40 nurses each year from 10 cities and 100 country hospitals affiliated with Shanxi Quality Control Center to train in the 1st and 2nd affiliated hospital of Xi`an Jiaotong University were choosen as 214 PICC maintainers. These maintainers were taken into the inner-net of 1st affiliated hospital of Xi`an Jiaotong University management system complete the PICC maintenance net of Shanxi province. Results After the establishment of maintenance network, the number of patients with PICC increased (from 610 cases in 2008 to 1 434 cases in 2014);A large number of PICC management had been down by the qualified nurses in primary hospitals; Duration of PICC in patients days was prolonged; The PICC related complications including thrombophlebitis, catheter infection,occlusion or dislodgment and unplanned extubate were significantly decreased (P < 0.05 respectively). Conclusions Establishment of PICC maintenance network can provide patients with standardized and convenient PICC maintenance service,decrease the incidence of catheter which related complications and ensure patient safety. The application of PICC maintenance network should be used more and more.

Objective To investigate the value of CT and MRI in diagnosing facial nerve neuroma. Methods The CT and MRI findings of facial nerve neuroma proved by surgery and pathology in 6 cases were retrospectively analysed. Results Four of the six facial nerve neuroma only affected intratemporal segment of facial nerve (labyrinthine segment 1, tympanic segment 2, mastoid and tympanic segment 1), one involved the cerebellopontine angle(CPA) cistern, internal anditory canal(IAC) and intratemporal segment and last one involved both the intratemporal segment and the intraparotid gland segment. The imaging manifestations of the tumor depended on its location and extension. On CT, the tumors of intratemporal segment showed enlargement and destruction of facial nerve canal, soft tissue mass in the middle ear and /or in the mastoid, erosion of the aterior surface of the petrous bone at the level of the geniculate ganglion fossa, and extension to the middle cranial fossas and intraparotid gland. One neuroma arising from IAC and cisternal segments demonstrated a mass in the CPA, widening of the IAC, enlargement of the labyrinthine segment of the facial nerve canal, and extension to geniculate ganglion fossa by CT and MRI. Conclusion CT and MRI are accurate to describe the extent and location of facial nerve neuroma. CT is better to demonstrate the osseous destruction in detail, whereas enhanced MRI evaluates the tumor itself more accurately.

Objective To study the SEN-V DNA in liver tissue from patients with non A to E hepatitis in Guangzhou area. Methods A set of primers according to the original sequence from GenBank were designed , and SEN-V DNA sequence was amplified by in situ-PCR in liver tissue. Results Two of 6 patients with non A to E hepatitis were found to be SEN-V-DNA positive in liver tissue. While 8 patients with HBV infection and 6 of other liver diseases including 2 of autoimmune liver disease, 1 of Dubin Johnson syndrome, 1 of fatty liver, 1 of the primary biliary cirrhosis and 1 of drug induced hepatitis were all SEN-V DNA negative. SEN-V DNA were mainly distributed in liver cell cytoplasm, partially in nucleus. There presented the phenomenon of cell fuse. SEN-V positive cells accounted for about 3%～10% of total cells.Conclusions SEN-V can be found in liver tissue of patients with non A to E hepatitis in south China. Maybe it can cause liver damage.

[Objective]To treat the femoral neck fracture using cannulated screw and BMP release system in animals,and to evaluate the possibility of treating femoral neck fracture and provide experimental basis for clinical application.[Method]Eighteen mongeral were used in this study.The model of bilateral femoral neck dislocation fracture was established.The control side was fixed using cannulated screw,and the experiment side was fixed with cannulated screw and injectable BMP release system.At 4,8,12 weeks,6 animals were sacrificed at one time point respectively.Results were obtained through histology,radiography,scintimetry and gross observation.[Result]The fracture line was vague at four weeks,and at eight weeks the fracture line almost disappeared.It was healed completely at twelve weeks.Radiological study showed that the healing of the fracture in experimental side was better than that of the control side.There was the same result of observation in histology.[Conclusion]The cannulated screw combined with BMP used in the experiment is effective and feasible.It may not only provide strong internal fixation but also infuse growth factor into site of fracture.It would accelerate the reconstructing of the vascular supply to the femoral head after the fracture and promote the restoration of bone.

Objective To investigate the relationship between Helicobacter pylori(H. pylori)infection and gastric metaplasia in the duodenal bulb and to pursue whether they play critical roles in pathogenesis of duodenitis and ulcer.Methods Eighty-two archive paraffin blocks of duodenal biopsy were obtained. All sections were stained with H-E, AB/PAS and Giemsa stains for histology, gastric metaplasia and H. pyloriassessment. There were 10 patients with normal duodenum, 47 with duodenitis and 25 with ulcer confirmed by endoscopy. Results There was a discrepancy in diagosis of the normal duodenal bulb mucosa between endoscopy and histopathology. Mild to moderate infiltration of inflammatory cell without gastric metaplasia were detected in 60% of cases with the normal duodenal bulb mucosa judged by endoscopy. Gastric metaplasia in duodenal bulb was the major phenomena in the patients with duodenitis and ulcer (37/82, 45%). H. pyloriinfection in the duodenal bulb always appeared in areas of gastric metaplasia. H. pyloriwas identified in 28 out of 37 (76%) cases in the gastric metaplasia mucosa. The prevalence of gastric metaplasia in the duodenal bulb between the patients with ulcer (72%) and duodenitis (40%) was significantly different (P=0.0078). The frequency of H. pyloricolonization was higher in the patients with duodenal ulcer (89%) than the patients with duodenitis (63%), but did not reach statistical significance(P=0.062). H. pyloriinfection was also higher in the ulcer patients with active, healed or scar stage, being 9/10, 5/6 and 2/2, respectively. Conclusions There is a difference in the frequency of H. pyloricolonization in the gastric metaplasia mucosa in the patients with ulcer and duodenitis, which suggests that infection with H. pylorimay play an important role in ulcer recurrence.