In recent years, research about microRNA has been increasing.How to safely and effectively introduce microRNA into target cells and target tissues is the key to successful treatment of diseases using microRNA.Many studies have reported that ultrasound-targeted microbubble destruction technology (UTMD) mediated gene transfection has the advantages of safety, targeting and high transfection efficiency.The research of UTMD mediated microRNA transfection has also increased year by year.The mechanism of UTMD mediated microRNA transfection and the application in the treatment of diseases such as cancer and cardiovascular system are reviewed in this paper.

Objective To investigate the effect of Tetramethyl Pyrazine(TMP) for treating cerebral infarct by observing infarct areas,and to research its function mechanism by observing the changes of the expressions of ED1,TNF-? and IL-1? in the infarction region after focal cerebral ischemia reperfusion injury.Methods Sixty SD male rats in healthy condition were randomly and averagely divided into normal group,model group and TMP high,middle and low dose group.The cerebral infarct animal model was reproduced by modified thread-tie method.TMP high dose group was administered TMP 140 mg/kg,middle dose group was 120 mg/kg and low dose group was 100 mg/kg by intravenous.Then all the rats were killed.Thirty rats were taken to evaluate whether TMP can decrease the ratio of cerebral infarction areas in the TTC-reacting and formalin fixation brain tissue slices taken from the above-mentioned killed rats,with Pathology Image Analysis System.The masculine of ED1,IL-1? and TNF-? in the immuno-reacting cells in cerebral infarction brain tissue slice taken from other thirty killed rats were observed.Results TMP groups decreased the ratio of cerebral infarction areas,and also decreased ED1,TNF-? and IL-1? in immuno-reacting cell.Conclusion TMP can decrease the formation of cerebral infarction caused by cerebral ischemia reperfusion and its effect is possibly related to the activation of microglia,as well as the immuno-positive expressions of TNF-? and IL-1?.

Autoimmune disease is a condition arisen from an abnormal immune response to the tissue cells itself, its precise mechanism remains unknown, and the failure to distinguish self from non-self is often termed a breach of tolerance and is the basis for autoimmune illness. The tumor necrosis factor-α (TNF-α) induced protein 8 like-2 (TIPE2) is a newly discovered member of TNF-α induced protein 8 (TNFAIP8) family which is an essential negative controller of both innate and adaptive immunity. It has been documented that marked expressions of TIPE2 are evident in various autoimmune diseases, including autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), myasthenia gravis (MG) and systemic lupus erythematosus (SLE), which appear to be closely related to the severity, progression as well as prognosis of the illness, thereby contribute to the pathogenesis of autoimmune diseases. Deficient expression of TIPE2 might contribute to the hyper-reactivity of auto-reactive lymphocytes and macrophages, or aggregate inflammatory reaction by prompting high concentration of pro-inflammatory cytokines in peripheral blood, thus, trigger the development and progression of autoimmune diseases. In addition, dysregulation of immune homeostasis could be another latent target involved into the mechanism of autoimmune diseases. The present paper summarized the potential role and its mechanism of TIPE2 in the development of autoimmune diseases.

Ultrasound molecular imaging has become one of the hotspots of molecular imaging because of its advantages of non-invasion, non-radiation, repeatability and real-time dynamic imaging. The key of imaging is to select the appropriate target and ligand to bind to the ultrasound contrast agent. The formation of blood vessels in the development of tumor is a significant feature. Vascular endothelial growth factor receptor (VEGFR) is an important specific molecule on tumor vascular endothelial cells, and which can be used as a target for tumor ultrasound imaging. In recent years, many scholars have carried out related studies on the imaging of ultrasound molecules targeting VEGFR. This paper reviewed the mechanism and application of tumor ultrasound molecular imaging with VEGFR as target in recent years.

Diabetes is non-infectious chronic disease which is associated with life style,and mental stress is an important pathogenic factor of it.The article explained experience of Professor JIN Hong-yuan in treating diabetes from seven emotions,and approached application of mentation in diabetes from etiopathogenisis,pathogenesis,diagnosis,treatment,health cultivation and so on,at last to elevate effectiveness in clinic.So it had important significance to elevate existence quality and overall-controlled patient's condition through modern psychological support therapy combining with recognition of JIN Hong-yuan on seven emotions.

Infectious diseases are resulted from the invasion of an organism's body tissues by multiple disease-causing agents. It has been demonstrated that the occurrence and development of infectious diseases are closely associated with the functional status of immune system. Cytokines play significant roles in modulating the host immune response to the clearance of pathogenic microorganisms and maintaining immune homeostasis. Interleukin-35 (IL-35), as a newly identified member of IL-12 family, exerts suppressive effect on immune response by means of a specific pattern. With the progress of research in recent years, IL-35 might serve as an essential contributor in the immunopathogensis of vast infectious diseases, including hepatitis B, sepsis, tuberculosis and parasite infection, which simultaneously appear to be closely related to the severity, progression as well as prognosis of the illness. Apparently, IL-35 is regarded as a potent and promising anti-inflammatory cytokine in clinical application; its potential value may shed light on the therapeutic strategies for infectious diseases. Herein, we mainly review the potential role and its mechanism of IL-35 in the pathogenesis of infectious diseases.

Objective To observe the protecting effect of Tongluoningxinfang decoction on human umbilical vein endothelial cells. Methods To cultivate endothelial cells and prepare serum with Tongluoningxinfang decoction and Fufangdanshenwan,cells were randomly separated into 8 groups,normal group,model group,treat group (high dose,medium dose,low dose),control group (high dose,medium dose,low dose). Except normal group,add 30% H2O2 to the other groups,and make endothelial cells damaged models. Add serum with drugs to treat groups and control groups,and test NO,ET-1 and P-selection (Ps) respectively. Result NO of model group was significantly lower than normal group,all treat groups and all control groups. ET-1 of model group was significantly higher than all treat groups and all control groups. Ps of model group was significantly higher than normal group,treat groups (smedium dose,low dose) and control groups (high dose,medium dose). It had no significant difference compared with model group,treat group (high dose) and control group (low dose). Conclusion Tongluoningxinfang decoction has the same influence as Fufangdanshenwan on secretion of vein endothelial cells. Tongluoningxinfang decoction and Fufangdanshenwan all can prevent vein endothelial cells from damage induced by H2O2.

Objective To understand the knowledge,attitudes and impact factors related to voluntary counseling and testing(VCT)for HIV/AIDS among migrants in Minhang district of Shanghai.Method Using a convenience sampling strategy,74 migrants,27 key informants and 20 community service providers were selected to participate in in-depth interviews and focus group discussions.Results Most migrants were young,less educated and poorly aware of HIV prevention.Only one third of the migrants heard of VCT,but few of them knew where to seek VCT or had any personal VCT experience.Nearly half of the interviewees took a negative attitude towards those who participated in VCT service.The main factors influencing the participation in VCT service among the migrants were the knowledge of VCT,having risk behavior and perception,supportive environments and availability of HIV/VCT services.Conclusions There is an urgent need to promote extensive health education about HIV/VCT,adopt flexible and available approaches to VCT service and provide appropriate care following the HIV test among migrants.

Objective: Establishment of information system can contribute to the integration of resources and process optimization of the hospital, so that it can reduce operating costs and improve service quality, work efficiency and the management level. It is helpful to realize the digital hospital and is the new trend of modern medical development. Methods: Information systems using C/S and B/S mode with full module function. The navigation is clear, flexible and easy to use;which have reserved for special interface data platform for easy to expand. Results:Through the implementation of information systems to solve a single asset management tools, please collar complex, information isolated, accounting information lag and other issues, to improve efficiency of hospital equipment and patient satisfaction guarantee, to achieve a transparent, integration, dynamic, precise management of hospital assets. Conclusion: The system realizes quality and safety control to the assets of whole process, specialization and informatization. The system adapts to the real service of hospital asset management, to meet the requirements of asset information management in the hospital.

Objective: To investigate the effect of helper T cells (Th1 and Th2) and its important significance in judging prognosis of patients with advanced lung cancer. Methods: Interferon-? (INF-?) and interleukin-4 (IL-4) in plasma and pleural effusion were detected by enzyme-linked immunoassay in patients with advanced lung cancer, IFN-? and IL-4 reflected activity of Thl and Th2 respectively. Results: The activity of Th1 in patients with advanced lung cancer was lower than that in normal persons in peripheral blood. For non-outstanding curative effect or progressive state of illness, the activity of Thl in patients of above 1 year survival time decreased in post-treatment than in pretreatment, the activity of Th2 increased in post-treatment. Conclusion: Activity of Helper T cells ( Th1, Th2 ) could be an important marker to diagnose lung cancer and judge prognosis in patients with advanced lung cancer.