ObjectiveTo observe the effects of high mobility group box-1 protein ( HMGB1 ) on function of murine spleen IL-10-producing dendritic cells (DCs) subset CD11clowCD45RBhighDCs.MethodsSplenic CD11clowCD45RBhighDCs and CD4 +T cells in Balb/c mice were purified by magnetic beads sorting.CD11clowCD45RBhighDCs were treated with various doses of HMGB1 (0,20,100,500ng/ml).Flow cytometry was used to determine expressions of CD11clowCD45RBhighDC surface molecules including CD40,CD80,CD86,Ⅰ-a/e and Toll-like receptor (TLR) 4.IL-10 level in CD11clow CD45RBhighDC culture supernatants was determined by ELISA method.The CD4+ T cells were divided into four groups: control group (without only treatment),untreated group (HMGB1-untreated CD11clow CD45RBhigh DCs plus CD4+T cells),high-dose HMGB1-treated group (500 ng/ml HMGB1-treated CD11clowCD45RBhigh DCs plus CD4 +T cells),high-dose HMGB1-treated + antibody 1 group (500 ng/ml HMGB1-treated CD11clowCD45RBhigh DCs plus IL-10 antibody and CD4+T cells),high-dose HMGB1-treated + antibody 2 group (500 ng/ml HMGB1-treated CD11clow CD45RBhigh DCs plus homotype IL-10 antibody and CD4 +T cells).IL-4 as well as interferon( IFN-γ) contents in CD4 + T cell culture supernatants were determined by flow cytometry.ResultsHMGB1 could markedly enhance the expressions of CD40,CD86 and TLR4 on CD11clowCD45RBhigh DC surface as well as level of IL-10 secreted by CD11clow CD45RBhigh DCs as compared with the non-HMGB1 treatment.Meantime,the secretion of IL-10 was HMGB1 concentration dependent.IFN-γ level in high-dose HMGB1-treated group was (279 ± 17) pg/ml,which was markedly lower than that in the untreated group [ (963 ± 11 ) pg/ml,P ＜ 0.05 \ ].IL-4 level in high-dose HMGB1-treated group was (372 ± 14) pg/ml,which was significantly higher than that in the untreated group [ (213 ± 10) pg/ml,P ＜0.05) \].ConclusionsHMGB1 promotes IL-10 production in CD11clowCD45RBhighDCs,induces the differentiation of CD4 +T cells to Th2 cells and downregulates the immune response via activating CD11clowCD45RBhigh DCs.

Objective To compare the treament to adhesion ileus between laparoscopic surgery and traditional surgery.Methods 59 cases of patients with adhesion ileus were selected.32 patients were given laparoscopic adhesion ileus loose solution method.And the other 32 patients were given traditional surgery.Results The operation time(t =12.135,P =0.002),peristalsis recovery time (t =9.053,P =0.001),postoperative infection rate (x2 =8.346,P =0.004),obstruction again (x2 =4.863,P =0.026) and hospital stays (t =4.724,P =0.028) in laparoscopic surgery group was significantly better.The differences between both groups were statistically significant (P ＜0.05).Conclusion Compared with the traditional open surgery,laparoscopic adhesion ileus loose solution with surgical trauma is small.Operation time and the postoperative recovery time is short,It can be used as the main treatment of adhesion ileus.

Transcutaneous immunization (TCI) is a novel immunotherapy approach that induces systemic immune responses via topical application of antigens and adjuvants onto the skin.It is a safe and effective method,and is expected to serve as an attractive alternative to traditional vaccination.TCI induces immune responses in different directions,but the exact mechanism of skin immune tolerance is still unclear.Most studies on TCI are based on animal models.However,there are structural differences between animal skin and human skin,so more researches are needed to achieve the translation of TCI from bench to bedside.Not all vaccines are suitable for cutaneous inoculation,so vaccines for TCI should be evaluated for their safety and efficacy.Various reformed vaccine delivery systems are needed to be further explored in related diseases.

The principle of R0 resection for colorectal cancer(CRC)has been widely accepted based on the preoperative imaging evaluation.The surgeons should scientifically and strictly assess resectability of tumors and furthest reduce tumor residuals.Patients with initial unresectable CRC will give priority to conversion therapy and then undergo R0 resection if converting into resectable tumors.Palliative operation for the patients who cannot receive R0 resection according to the clinical assessment should strictly follow surgical indications and achieve wellplanned and clear surgical purposes.There is no evidence that the palliative operation or cytoreductive surgery benefits to survival of patients,and decreasing complications and improving quality of life through minimum wound are the basic principles.

Objective To investigate the inhibitory effect of RNA interference(RNAi)on WT1 gene expression in HL-60 cell line.Methods Several pairs of small interfering RNA(siRNA)for WT1 gene was designed and transfected into HL-60 cells.WT1 gene expression was assessed by RT-PCR,and WT1 protein expression was tested by Western blot.Flow cytometry was used to measure the cell apoptosis.Results The expression rates of WT1 mRNA and the expression rates of WT1 protein were(23.62%?1.28%),(23.20%?1.04%),respectively in HL-60 WT1 siRNA-transfected cells,significantly lower than those of control cells(P

Leptin,a polypeptide hormone coded by Ob-gene,has the functions of depressing appetite,reducing caloric intake and enhancing caloric consumption,etc.Leptin has a close relationship with kidney,for it is metabolized and eliminated via kidney.Meanwhile,leptin can also directly affect kidney functions,influencing the process of chronic renal failure.Furthermore,hyper-leptinemia is associated with anorexia,malnutrition and cardiovascular diseases.Different dialysis patterns may also play different roles in cleaning leptin.For example,common dialysis could not clear leptin,but other dialysis modes such as high permeability dialysis,hemodiafiltration,and mastic absorption hemoperfusion can alleviate hyper-leptinemia through cleaning leptin partly.For kidney transplantation patients,hyper-leptinemia may affect the immune balance which has been maintained for a long time.

Dioxin,as a kind of strong carcinogenic substance,a kind of environment hormone with tremendous harm,can get into human body through food chain and accumulate in fat of animals and persons,forming serious threat to human health. Dioxin has been included in the list of monitoring objects in food part of the global environment monitoring program as a new environmental pollutant by World Health Organization. In this paper,the toxicity,the source of dioxin and its impact on human health and prevention and control measures are introduced.

Objective To investigate the clinical manifestations, histopathological patterns and causes of misdiagnosis of primary malignant tumor in small intestine (PMTSI). Methods The clinical data of 90 cases of PMTSI confirmed by pathology admitted from 1981 to 2002 in our hospital were retrospectively analyzed. Results The main pathological patterns of PMTSI were adenocarcinoma, leiomyosarcoma, lymphoma and malignant neurilemmoma. The common clinical manifestations were abdominal pain, anemia and hemorrhage, jaundice, weight loss and intestinal obstructions. Preoperative diagnosis rate was 22.2%(20/90). It was easily misdiagnosed as other digestive tract diseases and pelvic disorders. Preoperative misdiagnosis rate was 77.8%. Conclusions The main pathological patterns of PMTSI are adenocarcinoma, leiomyosarcoma, lymphoma and malignant neurilemmoma.Its clinical manifestations and diagnostic examination methods are not good enough, which usually leads to misdiagnosis ,so attention must be paid.

Differentiating bipolar disorder (BD) from unipolar depression (UD) is an important clinical challenge.Review the development of Magnetic Resonance Imaging ( MRI) in distinguishing the BD and UD, identifying objective markers of BD, to optimize clinical decision making.Database including PubMed,Wan Fang,CNKI and so on.The key words were usedunipolar depressionormajor depressive depression,bipolar depression,MRI,modeland so on.A little neuroimaging studies to date have directly compared UD and BD depressions.Most results from these studies suggest more heavy neural circuit abnormalities in BD than UD depression,involved in different brain regions.Predictive models based on neu-roimaging characteristics of BD and UD obtain a higher accuracy and can help differentiate BD from UD.This review serves as a call to highlight the need for more neuroimaging studies to compare individuals with BD depression with individuals with UD depression directly.Using neuroimaging results as objective biological i-dentification markers is a feasible research field.

Objective To investigate the protective effects of glycine on the lung injury in acute necrotizing pancreatitis(ANP). Methods Male Wistar rats were randomly divided into 4 groups: sham operation, ANP group,pretreated group and treatment group(with intravenous glycine 1g/kg). Serum TNF ? level,glutathione (GSH) and myeloperoxidase (MPO) in pulmonary homogenate were determined at 6, 12, and 24h hours after ANP induced. Pulmonary pathology and 3 day survival rate were compared between the groups.Results Compared with ANP group,in glycine pretreated group serum TNF ? level significantly reduced at 6,12, and 24h,while in glycine treatment group serum TNF ? level decreased at 24-hour . In glycine pretreated group or treatment group, the pulmonary GSH content were decreased.In both glycine pretreated group and glycine treatment group, pulmonary MPO activity decreased at 12 and 24h,and pulmonary pathologic severity significantly ameliorated and survival rate increased. Conclusions Although glycine can not increase the GSH synthesis,but giving prophylactic or therapeutic glycine may be effective in improving acute lung injury in ANP, probably through the inhibition of the infiltration and activation of leukocytes.