Narcolepsy is a common chronic sleep disorder attacking adolescence,characterized by a typical tetrad of excessive daytime sleepiness,cataplexy,sleep paralysis and hypnagonic/hypnopompic hallucinations,affecting both mental and psychological aspects of children.Thus,making an early diagnosis and therapy is of great importance.Clinical diagnosis mainly depends on the multiple sleep latency test across the video electroencephalogram.In this article,we mainly describe the diagnosis and treatment progress of pediatric narcolepsy and clinical value of video-electroencephalogram.

Objective To investigate the relationship between PreS1 antigen and five items of HBV serological markers(HBV M), as well as the clinical significance of combined measurement of PreS1 antigen and HBV M in patients with hepatitis B. Methods Enzyme linked immunosorbent assay (ELISA) was applied to testing serum in 268 patients with hepatitis B. Results The detection rate of PreS1 antigen was 85.88% in HBeAg'positive samples,significantly higher than that in HBeAg nega-tive samples(39.34%), P<0.01. Conclusion PreS1 antigen may effectively reflect the situation of HBV amplification. Combined measurement of PreS1 antigen and HBV serological markers in clinical examination contributes to clinical diagnosis,therapeutic effect monitoring and prognosis evaluation.

OBJECTIVETo systematically evaluate the effectiveness of integrative medicine (IM) for preventing postoperative recurrence of primary hepatic carcinomas (PHC).

METHODSSuch databases as The Cochrane Library, PubMed, EMbase, China National Knowledge Infrastructure (CNKI), VIP Database, Wan-fang Database were retrieved to collect randomized controlled trials (RCTs) on preventing postoperative recurrence of PHC by IM. The retrieval period started from database establishment to June 2013. Literature screening, data extraction, and quality evaluation were performed by two reviewers strictly according to inclusion criteria. Meta-analysis was conducted using RevMan 5.2 Software.

RESULTSA total of 5 RCTs involving 367 patient cases were included. Results of Meta-analysis showed that the IM group was superior to the Western medicine (WM) group in the 1-, 2-, and 3-year recurrence rates (P < 0.05).

CONCLUSIONIn the prevention of postoperative recurrence of PHC, the effectiveness of IM was more significant, when compared with treatment of Western medicine.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine ; Liver Neoplasms ; prevention & control ; Neoplasm Recurrence, Local ; prevention & control ; Postoperative Period ; Randomized Controlled Trials as Topic ; Treatment Outcome

Objective To reveal the research focus of schistosomiasis in China through analyzing highly cited schistosomiasis related papers in journals from 2005 to 2012,so as to provide the information for formulating the plan of selecting topics of the edi-tors. Methods The published articles related to schistosomiasis in CNKI from 2005 to 2012 were collected,the citation frequen-cy of the papers was calculated according to formula,and the highly cited papers were selected according to the citation frequency. Then the information of highly cited papers including the published time,type,author and author affiliation,citation frequency, journal,grant and research item,research status and development tendency were analyzed. Results A total of 3 639 published papers related to schistosomiasis were searched and 343 papers(the citation frequency≥8)were selected as highly cited papers, and Chinese Journal of Schistosomiasis Control shared 45.19%of them. Among these papers,original articles,reviews and special reports occupied 44.90%,12.54%and 6.12%,respectively. The first authors of these papers were mainly from centers for disease control and prevention(CDCs)(17.33%),research institutions(22.67%)and colleges/universities(36.00%). Totally 50.44%of the articles received financial supports from research funds/foundations,and most of them were national or provincial funds. The research hotspots focused on epidemiology and prevention,epidemic situation analysis,vaccine,immunologic diagnosis,pharma-ceutical research,immunity and infection. Conclusions The highly cited papers related to schistosomiasis are mainly from CDCs,institutions and colleges/universities. The research hotspots mainly focus on epidemiology and prevention,vaccine,immu-nology and pharmaceutical study. The related journals can use this information to chose topic and solicit contributions to improve their influence.

Multilocus sequence typing MLST with high solution sensitivity and specificity is widely used to study the population genetic structure of pathogen by amplification and sequencing of the housekeeping genes. MLST also provides more evidence and plays an important role in parasite research. This paper reviews the principle and method of MLST and its applica-tion on population genetic structure analysis of parasites.

Objective To explore the Troponin I(cTnI) ,myoglobin(MYO) and creatine kinase isoenzyme(CK‐MB) in early di‐agnosis of acute myocardial infarction(AMI) ,and compare their significance in the diagnosis of AMI .Methods used chemilumines‐cence method in 126 patients with AMI and 82 cases of myocardial infarction group to test cTnI ,MYO and CK‐MB ,the results were compared .Results The cTnI ,MYO ,CK‐MB quality appear time and peak time was different ,in patients with acute myocardial in‐farction(cTnI ,MYO and CK‐MB joint detection sensitivity ,specificity ,negative predictive value ,positive predictive value was higher than single test .Conclusion cTnI ,MYO and CK‐MB joint detection is helpful to early diagnosis of AMI .

Objective To analyze the highly cited malaria papers published in Chinese journals from 2006 to 2013,so as to provide the evidence for formulating the plan of selecting topics to the journal editors. Methods The published articles related to malaria included in CNKI and Wanfang medical network from 2006 to 2013 were collected,and the highly cited papers were selected according to the citation frequency calculated by Price’s formula. Then the characteristics of the highly cited papers were analyzed. Result From 2006 to 2013,a total of 1 976 published papers related to malaria were searched in Chinese jour?nals and 98 papers of them were selected as highly cited papers. In the highly cited papers,18 papers were published in China Tropical Medicine,and 16 and 15 papers were published in Chinese Journal of Parasitology and Parasitic Diseases and Chi?nese Journal of Schistosomiasis Control,respectively;and original articles accounted for 42.86%;the first authors of these pa?pers were from 44 institutions,and 40.91%of them were from centers for disease control and prevention(CDCs);a percentage of 22.45%of the highly cited papers received fund programs,and most of them were national or provincial funds. The research hotspots were focused on the epidemiology and control,and epidemic situation of malaria. Conclusion The highly cited papers related to malaria are mainly from CDCs and research institutions,and the related journals could use this information to chose topics and solicit contributions to improve their influence.

Objective To discuss the clinical features of non-conclusion seizures(NCS)in infants less than 1 -year-old,and the diagnostic and differential diagnostic value of video electroencephalography (VEEG).Methods A total of 30 NCS patients were took the regular electroencephalography at the first 20 minutes before VEEG monitoring which continued 2 to 4 hours in order to compare the differences between the two monitoring methods,then all patients were taken follow-up after one month,two months,and three months.Results Three cases attacks(accounting for 10.0%)were found by regular electroencephalograph-y,while 23 attacks(accounting for 76.7%)were found by VEEG following no epileptic discharge,either.All patients were found to be corporality NCS,having no psychogenic NCS,among which,19 patients(accounting for 63.3%)were non epileptic tonic-closure seizures,including 2 patients always keeping eyes staring at somewhere,2 patients gritted teeth or grinned,1 patient often put head back,1 patient stretched the neck or necking down,2 patients shrug his shoulders,9 patients shook head,and another 2 always put forth his strength, with or without stiffness,limb jitter,or made face red;on the other,7 patients (accounting for 23.3%)were benign myoclonus,another 4(accounting for 13.3%)were benign non epileptic infant spasm,no other types were found.Nobody had any antiepileptic therapies,15 of them have no more attacks in less than one month,7 in two months,1 in three months,and another 7 appeared occasionly under the conditions of great happiness,extreme an-ger or been raged.Conclusion VEEG have irreplaceable diagnostic and differential diagnostic value in infants less than 1 -year-old with NCS,and play an important role in monitoring the development of NCS.

OBJECTIVE:To study the effects of volatile oil of Pogostemon cablin on the expression of tight junction proteins ZO-1 and Occludin in colonic mucosal epithelial cells of rats with post-infectious irritable bowel syndrome(PI-IBS). METHODS:Rats were randomly divided into normal control group(distilled water),model group(distilled water),Trimebutine maleate tablet group(TMT,0.1 g/kg)and volatile oil of P. cablin low-dose,medium-dose and high-dose groups [2,3,4 g(crude drug)/kg] with 8 rats in each group. Except for normal control group,those groups were given colon perfusion of acetic acid to induce PI-IBS mod-el. After modeling,they were given relevant medicine intragastrically once a day for consecutive 5 days. The expression of ZO-1 and Occludin [by IOD] and their distribution were detected by immunohistochemistry. RESULTS:In normal control group,ZO-1 and Occludin evenly distributed on the top of enterocyte,manifesting as alveolate and punctiform;in model group,ZO-1 and Oc-cludin scattered on the top of enterocyte,showing uneven dyeing or fade;the distribution of ZO-1 and Occludin in volatile oil of P. cablin groups was similar to normal control group,and its dyeing was lighter than that of normal control group. IOD value of ZO-1 and Occludin in model group were lower than in normal control group(P<0.01);those of volatile oil of P. cablin high-dose group and the IOD value of ZO-1 of P. cablin medium-dose group and TMT group were higher than those of model group (P<0.05 or P<0.01). CONCLUSIONS:The volatile oil of P. cablin can up-regulate the expression of tight junction proteins ZO-1 and Occlu-din in colonic mucosal epithelial cells of rats with PI-IBS.

Objective To summarize the modified clinical technique and experience of simultaneous kidney-pancreatic transplantation (SKPT) with enteric drainage(ED). Methods Two patients with insulin-dependent diabetes mellitus and end-stage renal disease underwent SKPT with enteric drainage of exocrine secretions.The patients were treated with quadruple therapy including antithymocyte globulin (ATG) induction therapy,prednisone,FK506,and Mycophenolate-Mofetil(MMF), as maintenance immunosuppression. Results The two patients became insulin-independent after treated by small dose insulin for 5～10 days and Scr,BUN became normal 3～5 days after the operation.Until now all the grafts of the patients functioned well. Conclusions ED-SKPT is more effective than simultaneous kidney-pancreatic transplantation with bladder drainage (BD-SKPT).ED-SKPT is an effective method for treating type Ⅰdiabetes mellitus with uremia.Finer allograft and nicer HLA-typing can decrease complications.