OBJECTIVE: According to the holism in traditional Chinese medicine (TCM) theory, this manuscript explores the relativity of pathogenesis among hypertension, diabetes and hyperlipemia.DATA SOURCES: We applied computer to search articles in English on pathogenesis ofhypertension, diabetes and hyperlipemia in Medline between January 1985 and December 2004 by words "hypertension, diabetes, hyperlipemia, pathogenesis" .Besides, we also used computer to search articles in Chinese on pathogenesis of hypertension, diabetes and hyperlipemia in Chinese magazine full text database between January 1990 and December 2004 by words "hypertension, diabetes, hyperlipemia, pathogenesis".STUDY SELECTION: Primarily, articles on pathogenesis of hypertension,diabetes and hyperlipemia were selected in TCM and modern medicine respectively. Accepted standard were: ① Random control clinic test with methods of single blind, double blind or no blind; ② Nonrandom control test;③ Fore-and-aft control test; ④ Chapter in monograph. Eliminated standard:repeated research.DATA EXTRACTION: Totally 28 articles on pathogenesis of hypertension,diabetes and hyperlipemia were found in TCM and modern medicine, however, 12 articles were selected according to accepted standard, and 16 articles were eliminated due to repeated content.DATA SYSTHESIS: Totally 12 selected articles were analyzed on pathogeneses of hypertension, diabetes and hyperlipemia in TCM and modern medicine, and hinted internal affiliation of these three diseases from view of molecular biology.CONCLUSION: These three diseases can be regarded as a whole entity for study. High blood pressure, high blood sugar and high lipemia could be treated as different forms of the entity or in different developing courses of the whole. In a certain course it might present as one of the forms specifically. It might manifest high blood pressure mainly in clinic, or high blood sugar, or high lipemia,also several forms at the same time. It will be beneficial to take the holism and visceral theory in TCM for reference in research of modern medicine.

Intrauterine growth retardation (IUGR) can improve the danger of insulin resistance syndrome. It was reported that regulatory factors of βcells development and enzymes of regulating metabolism of glucose, fat, insulin signal transduction and effecter molecule were expressed at a low level in the IUGR. As a result, the number of β cells, proliferation, insulin secretion and insulin sensitivity was reduced, apoptosis was increased, and glucose intake in peripheral tissue declined. All these factors contribute to the insulin resistance syndrome.

Objective To study the influence of Orem self-care theory on postpartum quality of life and role change of parturients with natural labor. Methods 100 parturients with natural labor in our hospital from May 2010 to July 2011 were selected as research object,and they were randomly divided into the control group and the observation group with 50 cases in each group,the control group was taken care with routine nursing methods,the observation group was given with nursing according to Orem self-care theory.Then the SCL-90 score,situation of role change and satisfaction degree of two groups before nursing and on the second and fifth days after nursing were analyzed and compared. Results The SCL-90 score of the observation group after the nursing on second and fifth days was all lower than those of the control group,the better rate of role change was higher than that of the control group,patients'satisfaction degree was higher than that of the control group. Conclusions The Orem self-care theory plays an important role in improving the quality of life and role change of parturients with natural labor after delivery,and it can significantly improve satisfaction degree of parturients.

OBJECTIVE:To establish an HPLC method for determination of major component and related peptide in eptifibatide injection.METHODS:The determination was performed on AichromBond-AQ C18 column with column temperature at 25 ℃ and flow rate of 1.0 mL?min-1.The mobile phase consisted of acetonitrile-water(0.13% trifluoroacetic acid).The detection wavelength was set at 220 nm and injection volume was 20 ?L.RESULTS:The linear range was 75.2～1 203.6 ?g?mL-1(r=0.9999) with mean recovery of 99.73%～99.95% (RSD=0.26%).CONCLUSION:The method is simple and rapid and it is applicable for determination of major component and related peptide in eptifibatide injection.

Objective:To explore the application effect of risk management in quality control of medical apparatus and instruments of operation room.Methods: 172 pieces of medical apparatus and instruments were divided into risk management group (n=86) andcommon management group (n=86). A series of indicators, such as effective acceptance rate, qualified cleaning rate, completed supporting facility, specific aim rate, deficiency rate of quantity, error rate of preparation, wet pack rate of sterilization, lost rate of post surgery and the satisfaction degree of physician, were applied to complete statistic analysis.Results: The effective acceptance rate, qualified cleaning rate, completed supporting facility rate and specific aim rate of risk management group were significantly higher than that of common management group (x2=5.02,x2=7.38,x2=9.35,x2=11.14,P<0.05). The deficiency rate of quantity, error rate of preparation, wet pack rate of sterilization, lost rate post surgery of risk management group were significantly lower than that of common management group (x2=6.63,x2=9.21,x2=11.34,x2=13.28,P<0.05). And the satisfaction degree of physician of risk management group (97.7％, 84/86) was significantly higher than that of common group (73.3％, 63/86) (x2=12.83, P<0.05).Conclusion: The application effect of risk management is better than that of common management in the quality control of medical apparatus and instruments in operation room.

Objective] To observe the influence of YIGU oral liquid on the pain threshold and bone mineral density in ovariectomized rats. [Methods] Eighty-four Female SD rats were randomly divided into 4groups:YIGU oral liquid group, calcitonin group,YIGU oral liquid combined with calcitonin group, saline control group.The ovariectomized rats were confirmed the successful model of osteoporosis,rats in the YIGU oral liquid group were administered with YIGU oral liquid, rats in the calcitonin group were administered with calcitonin, rats in the YIGU oral liquid combined with calcitonin group were administered with YIGU oral liquid group and calcitonin,rats in the saline control group were administered with saline.To detect the pain threshold and bone mineral density at the 10th day, 30th day, 60th day and 90th day respectively. [Results]At the 10th, 30th, 60th and 90th day after treatment, the pain threshold of the YIGU oral liquid group, calcitonin group,YIGU oral liquid combined with calcitonin group compare to saline control group, there was significant statistical difference( P<0.05).At the 10th, 30th day after treatment,the bone mineral density of the YIGU oral liquid group, cal-citonin group,YIGU oral liquid combined with calcitonin group compared with saline control group, there was no significant statistical difference( P>0.05). At the 60th,90th day after treatment, there was significant statistical difference( P<0.05).[Conclusion] YIGU oral liquid can effectively enhance pain thresh-old and bone mineral density of the ovariectomized rats to inhibit the osteoporosis pain.

Objective To explore the CT,clinical,and pathological features of eosinophilic lymphogranuloma(ELG)in head and neck region. Methods CT,clinical,and pathological data of 9 cases of ELG in head and neck region,confirmed by biopsy were retrospectively an-alyzed.Results Parotid gland was lonely involved in 3 cases,and cervical lymph nodes were lonely involved in 2 cases,and they were both involved in 4 cases.All of the patients suffered from painless nodule or mass in head and neck region with peripheral blood eosinophilia.On CT examination,nodular lesions of parotid gland were clear or slightly blurred defined with moderately or obviously homogeneous enhancement,and diffuse mass lesions in parotid gland were ill defined with involvement of surrounding tissue,and heterogenous progressive enhancement.The involved cervical lymph nodes were enlarged with clear boundaries,and moderately or obviously homogeneous enhancement.The pathological findings of ELG included the marked infiltration of eosinophilia,lymphocytic proliferation,lymphatic follicles,variable degrees of fibrosis and vascular proliferation.Conclusion ELG in head and neck region has some CT features,and diagnosis could be suggested combining with the clinical manifestations and peripheral blood eosinophilia. The final diagnosis depends on histopathological examination.

Objective To investigate the possible mechanism of apoptosis effect induced by resveratrol in human acute T lymphoblast leukemia Molt-4 cells. Methods MTT method was used to analyze the inhibition rate. The cell cycle and apoptosis percentage of Molt-4 cells treated with resveratrol were detected by flow cytometry. The expression of WAVE1 mRNA was assessed by semi-quantitative RT-PCR. Results After treated with 12.5, 25.0, 50.0, 100.0, 200.0μmol/L resveratrol for 24, 48 and 72 hours, the inhibitory effects were at 29.32 %, 36.11 %, 53.92 %, 62.50 %, and 74.98 %, respectively. Resveratrol was able to inhibit the proliferation of Molt-4 cells in a time-dependent and dose-dependent manner (F =33.614, P <0.05).Compared with control group, after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the cell number in S phase of Molt-4 cells was 68.6 % and 78.1 %, respectively, and the cell cycle was arrested at S phase (F = 19.453, P < 0.01) after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the ratio of WAVE1/GAPDH was 0.356±0.03, 0.382±0.05. Compared with control group 0.586±0.06, the ratio of WAVE1/GAPDH was decreased (F =8.950, P <0.01). Conclusion Resveratrol could induce the cells to undergo apoptosis, which was probably related to downregulation the expression of WAVE1.

Obesity and its related metabolic diseases become major health problems in the world.Adipose tissue plays an important role in the development of obesity.FSP27,a member of the CIDE family proteins,is expressed at high levels in white adipose tissue and differentiated 3T3L1 cells.The objective of current study is to establish a FSP27 knockdown preadipocyte cell line to investigate the in vivo function of mouse FSP27.The double strand siRNA of mouse FSP27 corresponding to nucleotides 270 to 291 was synthesized and inserted into pSilencer2.1.pSilencer-siFSP27 was co-transfected into 293T cells with the HA-mFSP27 expression vector to test its knock-down efficiency.The FSP27 siRNA was then transferred to a lentiviral vector.Lentivirus were generated and used to infect 3T3-L1 cells.It was shown here that lentivirus containing FSP27siRNA can effectively knockdown FSP27 expression in 3T3-L1 cells.Establishment of FSP27 knock-down cell line provides a useful tool for the study of in vivo function FSP27.

Humans ; Tuberculosis, Hepatic