Objective To reveal the effects of circadian gene Per2 on growth of human lung cancer cell A549 cell and its mechanism. Methods pcDNA3.1(+)-Per2 was transfected into A549 cells and controlled with the vector pcDNA3.1(+). The expression of Per2 was confirmed with RT-PCR and western blot. The Inhibitory effect of Per2 on cell growth and proliferation was demonstrated with MTT assay and colony-forming assay. Apoptosis was detected with flow cytometry. Results The circadian gene Per2 exhibited a growth-inhibitory and apoptosis-inductory effect on A549 cells. Conclusion The results indicate that Per2 may inhibit A549 cell proliferation,which mechanism might involve cellular apoptosis.

Objective To compare the characters of circadian rhythm of the cardiovascular function between full-term and premature infants.Methods Blood pressure(BP),aortic blood velocity(ABV),aortic blood acceleration(ABA) and aortic strok distance(ASD) were continuously measured in full-term and premature infants for 48 h.Each time series was analyzed with 24-hour cosine curve-fitting method.Results The parameters of cardiovascular function in most of the full-term infants,including BP,ABV,ABA and ASD showed robust circadian rhythm,but most of premature infants did not show obvious circadian rhythm.The averages of those parameters were not different in full-term and premature infants,whereas the amplitude were significant different in the two groups.Conclusion It is indicated that the functions of the cardiovascular system was not different in full-term and premature infants,but circadian rhythm of cardiovascular system in premature infants has not perfectly developed.

Objective To compare the Cosine and Dip methods in describing the rhythm of blood pressure(BP).Methods The BP was measured with non-traumatic ambulatory blood pressure monitoring(ABPM) for 24 h and analyzed with Cosine and Dip methods.The characters of the circadian rhythm of BP were studied and two methods were compared.Results The ratios of mesor and amplitude(A/M) of systolic blood pressure(SBP) and diastolic blood pressure(DBP) were(7.78?0.42)% and(7.98?0.39)%,respectively.The majority of acrophases of BP was on daytime,especially between 10∶00 am and 3∶00 pm.The amplitudes of acrophase in different periods analyzed with Cosine method were not significantly different each other,whereas analyzed with Dip method to be significant difference.Conclusion There is difference between Cosine and Dip methods in describing the rhythm of BP,since rhythms of acrophases of some cases were inaccurately described with Dip method.

The present paper reports the effect of pancreatitis induced by cholecystokinin (CCK) on free-running rhythm of locomotor activity of the ICR mice, and analyzes the interaction of inflammatory diseases and acute pancreatitis with circadian rhythm system. In the study, the mice were modeled under different phases of acute pancreatitis in DD status (Double Dark, constant dark condition). By comparing of the inflammatory status and the indicators of rhythm before and after modeling of the running wheel activity group and the rest group, it was observed that the rest group showed more possibility of inflammation than the activity group did in ICR mice model of acute pancreatitis. In the rest phase model, the extension of the period is particularly longer. The results presented indicated that CCK-induced acute pancreatitis impacted free activity rhythm of ICR mice. Also in a free running model under different phase, the inflammation severity was proved significantly different. This study provides possible clues for the research of the pathogenesis of acute pancreatitis severe tendency.
Animals ; Cholecystokinin ; adverse effects ; Circadian Rhythm ; Mice ; Mice, Inbred ICR ; Motor Activity ; Pancreatitis ; chemically induced ; physiopathology

Objective To observe the efficacy and safety of high-dose amiodarone administered by continuously intravenous infusion for cardioversion of paroxysmal atrial fibrillation.Methods From 2008 to 2010,109 patients suffered from paroxysmal atrial fibrillation were treated with high-dose arniodarone (125 mg/h) administered by continuously intravenous infusion.Before cardioversion,patients were treated with low molecular weight heparin anticoagulant therapy,Laboratory tests on FT3,FT4,TSH,serum ions,etc,and coloured Doppler ultrasound imaging of heart,and ECG and blood pressure monitoring were carried out.Amiodarone hydrochloride injectio of 150 mg was mixed with sodium chloride 50 mL homogeneously,and then infused continuously by micro-pump in a rate of 41 mL/h until resume of sinus rhythm or infusion was kept up to 24 h.After successful cardioversion,as appropriate,the intravenous amiodarone was maintained in a rate of 0.5-1.0 mg/min for 6-12 h joined with oral amiodarone dosing,and the total dose was limited up to 3000 mg.Results A cohort of 104 (95.4％) patients had the restoration of sinus rhythm after cardioversion.The mean dose of amiodarone for cardioversion was (774.52 t 700.53) mg,and time required for cardioversion was (6.3 ± 5.55) hours.Conclusions The patients with paroxysmal atrial fibrillation are given high-dose amiodarone (125 mg/h) continuously intravenous infusion therapy and have high cardioversion success rate,less complications and side effects,as well as other advantages at the basic hospital.The method above has broad application prospects.

OBJECTIVETo investigate the etiologies and clinical features for bilateral acute sensorineural hearing loss (bi-ASNHL).

METHODSThe clinical data of 19 cases presenting with bi-ASNHL were retrospectively analyzed, including the clinical features, systemic examinations, laboratory examinations, audiology and radiology results, as well as the prognosis.

RESULTSThere were 15 non-otologic diseases in 19 patients, accounting for 78.9% of the total cases, most of which were disorders with multisystem and multi-organ disorder. The central nervous system diseases including fungal meningitis, tuberculous meningitis, and viral encephalitis in 3 patients. The clinical features of deafness were bilateral, progressive, accompanied with fever, headache, dizziness, nausea, vomiting and change of mental status. There was a decrease in speech recognition score (SRS), and speech recognition threshold (SRT) was obviously inferior to pure tone average (PTA) disproportionally. Diseases of immune system including antineural cytoplasmic antibody (ANCA)-associated systemic vasculitis (AASV), relapsing polychondritis (RP), rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) in 5 patients. They showed the characteristics of bilateral, progressive and simultaneous autoimmune disease. Hematological and endocrine system diseases including diabetes mellitus, leukemia, and thyroid hypofunction in 5 patients. The deafness had the characteristics of symmetry and progressivity. Otologic diseases including large vestibular aqueduct syndrome (LVAS) and sudden sensorineural hearing loss (SSNHL) in 4 patients; Drug-induced sensorineural deafness happened in 2 patients. After the treatment aimed at the causes, 1 case was cured, 3 patients were markedly effective, 7 patients were effective, and 8 patients were ineffective(including dead and refusal cases), with a total effective rate of 57.9%.

CONCLUSIONSThe most of bi-ASNHL cases are often associated with systemic diseases. Clinicians should analyze the history and clinical characteristics in detail, and complete specific laboratory examinations, audiology and imaging examinations in order to reveal the causative diseases. It should be treated aimed at the etiology.

Autoimmune Diseases ; Deafness ; Hearing Loss, Bilateral ; diagnosis ; etiology ; Hearing Loss, Sensorineural ; diagnosis ; etiology ; Humans ; Retrospective Studies ; Syndrome ; Vertigo ; Vestibular Aqueduct