ssociation of snoring with metabolic syndrome in the four ethnic populations in Boertala region of Xinjiang.

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Objective:To observe the effect of Allicin in cardial fibroblasts (CFs) proliferation and Collagen secretion,and to explore its role on TLR4/NF-κB signal pathway.Methods:CFs of neonatal Wistar rats were isolated and cultured ,then was stimulated with AngⅡ.CFs proliferation was measured by thiazolyl blue ( MTT) assay.The expression of collagenⅠ,collagenⅢ was measured by ELISA.mRNA expression of TLR4 and NF-κB were detected by reverse transcription-polymerase chain reaction ,protein expression of TLR4 and NF-κB were detected with Western blot.Results: Allicin could reduced MTT value of cardial fibroblasts ( P<0.01 ) , and inhibited expression of collagenⅠ,collagenⅢ(P<0.01),which in a dose-dependent manner.Allicin could reduced mRNA expression of TLR4 and NF-κB and protein expression of TLR 4 and NF-κB in CF induced by Ang Ⅱ ( all P<0.01 ) .Conclusion: Allicin can inhibit Myocardial fibrosis ,which mechanism is possible by inhibiting TLR 4/NF-κB signal pathway.

Objective To construct a ciaR gene-knockout (ΔciaR) mutant of Streptococcus pneu-moniae ( S. pneumoniae) and to investigate the effects of CiaR in CiaH/CiaR, a streptococcal two-component signal-transducing system, on the expression of genes encoding penicillin-binding proteins ( pbps genes) and cia-dependent small RNAs (csRNAs). Methods Electrophoretic mobility shift assay (ESMA) was per-formed to detect the recombinant CiaR (rCiaR)-binding pbps genes. A suicide plasmid pEVP3ciaR for ciaR gene knockout was constructed and then aΔciaR mutant was obtained through homologous recombination and insertion inactivation of the suicide plasmid, and screening with chloromycin. The mutant was identified using PCR and sequencing analysis. E-test was used to detect the minimal inhibitory concentrations ( MIC) of penicillin ( PCN) and cefotaxime ( CTX) against S. pneumoniae strains. Changes and differences in the expression of pbps genes and csRNAs in theΔciaR mutant and its wild-type strain before and after treatment with 1/4 MIC of PCN or CTX were detected using real-time quantitative RT-PCR. Results The rCiaR could bind to the promoter regions in pbp1a, pbp1b and pbp2b genes of S. pneumoniae. The ciaR gene in ΔciaR mutant was inactivated by insertion according to the results of PCR and sequencing analysis. After treatment with 1/4 MIC of PCN or CTX, the expression of pbps genes at mRNA level ( pbps-mRNAs) in theΔciaR mu-tant was significantly increased (P<0. 05), but the levels of csRNAs were significantly decreased (P<0. 05);whereas a significantly decreased pbps-mRNAs (P<0. 05) and increased csRNAs (P<0. 05) were observed in its wild-type strain. The result of E-test showed that the MICs of PCN and CTX against ΔciaR mutant were increased by 250-fold as compared with those against its wild-type strain. Conclusion The CiaR can enhance the drug resistance of S. pneumoniae to PCN and CTX through down-regulating the expres-sion of PBP1a, PBP1b and PBP2b and up-regulating the expression of csRNAs to inhibit the expression of PBPs.

Objective To analyze the correlation of traditional Chinese medical constitution types withcardiovascular risk factors in community hypertension patients. Methods We defined the constitution types of 1108 community hypertension patients, created personal health archives and provided routine examination for the subjects. After that, we input and analyzed the relevant data and results by computer. Results In 1108 cases of hypertension patients, 229 (20.67%) were defined as harmony physique, 879 (79.33%) as biased physique. In patients with biased physique, phlegm-dampness physique accounted for 25.54% (283 cases) , and then came yin deficiency physique and Qi deficiency physique, which accounted for 15.16% (168 cases) and 13.18% (146 cases) , respectively. The biased physique was dominated by single biased physique with a total of 590 cases ( 67.12%) , but the complex physique was also in large quantity, with a total of 289 cases ( 32.88%) . The complex physique consisting of two kinds of single physique was commonly seen in the patients with complex physique, accounting for 15.70%. The complex physique consisting of more kinds of single physique had less percentage. Phlegm-dampness physique was a kind of physique involving the most cardiovascular risk factors. Patients with phlegm-dampness physique had higher incidence of overweight, abnormal lipid level, family history of cardiovascular disease, lack of physical exercise and electrocardiographic abnormality, and the difference was statistically significant compared with other physique types ( P<0.05 or P<0.01) . Conclusion Phlegm-dampness, yin deficiency and Qi deficiency physique are the three commonly-seen constitution types in hypertension patients. Phlegm-dampness physique is the most commonly-seen biased physique and is also a kind of physique involving the most cardiovascular risk factors. Prevention and management in consideration with the constitution characteristics and cardiovascular risk factors will be a new prospective method for the treatment of hypertension patients in community.

Objective:To investigate different sleep duration and glucose and lipid metabolism levels in residents of a community in Urumqi.Methods:Using the 2 049 residents′ data of chronic metabolic disease in a community of Urumqi collected in May 2017, 1 822 subjects aged between 19-80 years with complete information were enrolled, their blood pressure, waist circumference, height, weight, body mass index were measured and recorded. Using oral glucose tolerance test to measure fasting and 2 h after meal plasma glucose, uric acid, HbA 1C, total cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C) levels were all tested. Results:(1)There were 363 (19.9%), 1 349 (74.0%), and 110 (6.1%) respondents with sleep time≤6.0, 6.1 to 8.0, and>8.0 h/d, respectively. There were statistically significant differences in age, education, and family income in groups with different sleep time ( P<0.05), while their gender, smoking status, and exercise status were not statistically significant ( P>0.05). The rates of overweight, obesity, abdominal obesity, high uric acid, and hypertension in people with different sleep durations were statistically different ( P<0.01). The rates of the above indicators were higher in the group of sleep time≤6.0 h/d than the other two groups. (2) Differences in diastolic blood pressure, systolic blood pressure, body mass index, abdominal circumference, total cholesterol, and LDL-C levels were statistically significant among different sleep duration groups ( P<0.05). Further comparisons of the above indicators among three groups with different sleep durations were performed ( P<0.05). The levels of the above indicators in the sleep time≤6.0 h/d group were higher than those in the other two groups. There were no significant differences in fasting blood glucose, glycated hemoglobin, uric acid, triglyceride, and HDL-C among the three groups. (3) Multivariate logistic regression analysis showed that groups whether or not adjusted of age, family income, and education level, sleep time≤6.0 h/d was related to abdominal obesity, and sleep time≤6.0 h/d was be a risk factor for abdominal obesity [Unadjusted: OR=1.48(95% CI1.04-2.08); Adjusted: OR=1.65(95% CI1.18-2.32; P<0.05]. Conclusion:Sleep time ≤6.0 h/d is associated with abdominal obesity, and sleep time≤6.0 h/d may be a risk factor for abdominal obesity.

OBJECTIVETo investigate the association of the 1891-1892del TC polymorphism of regulator of G-protein signalling 2 (RGS2) gene with hypertension in Xinjiang Kazakh population.

METHODSThe case-control study was performed in 444 cases and 489 controls. The genotypes of the individuals in the 1891-1892del TC locus were identified by TaqMan method.

RESULTSA significant association was observed between the1891-1892TC insertion/deletion polymorphism with hypertension in men (OR=1.698, P=0.03) and in the total population (OR=1.32, P=0.044). The mean systolic blood pressure and serum uric acid levels of the ID+DD carriers were significantly higher than that of the II carriers (adjusted, P=0.04 and P<0.01).

CONCLUSIONThe results suggest that the D allele of the 1891-1892TC insertion/deletion locus of the RGS2 gene might be an independent risk factor for hypertension in Xinjiang Kazakhs; and the polymorphism may have some influence on serum uric acid level in this population.

Adult ; Aged ; Asian Continental Ancestry Group ; ethnology ; genetics ; Blood Pressure ; Case-Control Studies ; China ; Female ; Genetic Predisposition to Disease ; Humans ; Hypertension ; blood ; ethnology ; genetics ; physiopathology ; Male ; Middle Aged ; Polymorphism, Genetic ; RGS Proteins ; genetics ; Risk Factors ; Uric Acid ; blood

Objective To investigate the relationship between thyrotropin(TSH)and urine iodine in pregnant women of Han and Uygur ethnic groups in People′s Hospital of Xinjiang Uygur Autonomous Region. Methods A total of 1568 pregnant who completed screening of TSH and urine iodine in People′s Hospital of Xinjiang Uygur Autonomous Region hospital from August 2014 to December 2017 were included in the study, 956 cases were Han and the other 612 were Uygur. Basic clinical data, serum TSH, thyroid peroxidase autoantibody (TPOAb), and urine iodine levels were retrospectively analyzed. Results (1) General results:The median urine iodine level was 162.6μg/L(53.4-539.3μg/L), and the distribution of urine iodine classification was iodine deficiency 42.9％(672/1568), iodine appropriate 36.7％(576/1568), iodine slightly high 17.1％(268/1568)and iodine excess 3.3％(52/1568)respectively.(2)The median urine iodine levels of Han and Uygur pregnant women were 169.1μg/L(54.6-583.4μg/L)and 156.3μg/L (53.1-539.3μg/L)respectively, and the difference was statistically significant(P<0.05).The distribution of urine iodine status in pregnancy between Han and Uygur was significantly different, which were 40.9％(391/956)vs. 45.9％(281/612)in iodine deficiency, 35.4％(338/956)vs. 38.9％(238/612)in iodine appropriate, 20.2％(193/956) vs. 12.3％(75/612) in iodine slightly high and 3.6％(34/956) vs. 2.9％(18/612)in iodine excess.(3)High serum TSH level proportion was significantly higher in Uygur ethnic group, early pregnancy, thyroid peroxidase antibody positive and anti-thyroglobulin antibody positive group when compared with Han, late pregnancy, thyroid peroxidase antibody negative and anti-thyroglobulin antibody negative groups (all P<0.05). There were no significant differences in different age groups and iodine nutrition groups(P>0.05).(4)There was no correlation between urinary iodine and TSH levels in all pregnant women(P>0.05),neither in Han or Uygur group. When further stratified by gestational age, age, and antibody level, there was a positive correlation between urine iodine and serum TSH level in Han pregnant women>30 years old(P<0.05), and there was a negative correlation in the third trimester in Uygur (P<0.05). When serum antibody level, gestational week and age were controlled. There was no correlation between urine iodine and serum TSH level in neither group. Conclusions (1)In Han and Uygur pregnant women, the median urine iodine level and the distribution of urine iodine classification between two ethnic groups are significantly different.(2)The correlation between urine iodine and serum TSH is not identified in Han or Uygur pregnant women.

Objective To investigate the status of increased blood parathyroid hormone(PTH)level among community residents in Urumqi,and to analyze its correlation with the metabolic parameters including serum Ca and P levels. Methods In May 2013,a cross-sectional survey was conducted,when 1 473 permanent residents in Urumqi, including 844 Han and 629 Uyghurs,were selected by cluster random sampling. PTH and 25-OH vitamin D[25(OH)D] levels were detected by chemiluminescence. Blood Ca, P, Mg, albumin, and creatinine (Cr) were also measured. Based on serum 25(OH)D level,the residents were divided into vitamin D deficiency(<20 ng/ml), vitamin D insufficiency(≥20 and<30 ng/ml),and vitamin D sufficiency(≥30 ng/ml)groups. According to the PTH level,the residents were divided into PTH<65 pg/ml group and PTH≥65 pg/ml group. The metabolic parameters including Ca and P were compared among different ethnic groups. Results In the surveyed population,the rate of increased blood PTH was 12.22%,being higher in females than that in males(13.82% vs 8.45%,P=0.004)and higher in Han Chinese than that in Uyghurs(19.87% vs 6.52%,P<0.01). The proportions of residents with vitamin D sufficiency,insufficiency and deficiency were 3.34%,9.06%,and 87.60% in Uyghurs,and 10.43%,29.98%, and 59.60% in Han,respectively. The rate of 25(OH)D deficiency was significantly higher in Uyghurs than that in Han(P<0.01),especially higher in female Uyghurs(91.43%). In vitamin D deficiency group,the rate of increased serum PTH was significantly increased,higher in Uyghurs than that in Hans(22.14% vs 6.56%,P<0.01),which revealed a negative correlation with 25(OH)D(r=-0.251, P< 0.01). Conclusion 25(OH)D deficiency is prevalent among the community population in Urumqi, with ethnic differences in serum PTH level and the rate of increased blood PTH,which might be associated with vitamin D deficiency.

Objective To understand the vitamin D status and its possible ethnic differences among Uygur and Han women of childbearing age in a community of Urumqi.Methods Based on the data of 2 080 patients with thyroid disease in a community of Urumqi,which were collected in May 2013,we selected the complete information of 652 Uygur and Han women of childbearing age (between 18-45 years),which included 367 Uygur women [average age (35.14± 6.91) years] and 285 Han women [average age (35.52± 7.10) years].The basic data of height,weight,and waist circumference were recorded.The serum level of 25-hydroxyl vitamin D [25(OH) D] was detected by chemiluminescence method.Results The serum 25(OH) D levels of Uygur and Han were (11.51±6.31) μg/L and (16.43±6.43) μg/L,respectively.The 25(OH)D level in Uygur women was significantly lower than that of Han women (t =-9.800,P＜0.001).The constituent ratio of vitamin D status was significantly different between Uygur and Han women (vitamin D severe deficiency:47.4％ in Uygur women and 12.3％ in Han women),deficiency (44.7％ in Uygur women and 64.6％ in Han women),insufficiency 5.7％ in Uygur women and 17.9％ in Han women),sufficiency (2.2％ in Uygur women and 5.2％ in Han women) (x2 =99.486,P＜0.001).Conclusion Serum 25 (OH) D deficiency is common in Uygur and Han women of childbearing age in Urumqi,especially among Uygur women.