Hepatic stellate cells (HSCs), also called Ito cells or lipocytes, are one of inherent liver nonparenchymal cell types located in the Dissé space between hepatocytes and sinusoidal endothelial cells, and account for up to 50%-80% of vitamin A in the form of lipid drops. The methods of primary HSCs isolation mainly focus on density gradient centrifugation combined with centrifugal elutriation, side scatter-activated cell sorting, UV-excited autofluorescence or antibody-based flow cytometry, etc., and will provide solid foundation for the research on physiological and pathological HSCs function. The research of this vitamin A-storing cells has developed and expanded vigorously. In physiological conditions, HSCs are quiescent and play pivotal roles in the synthesis of extracellular matrix (ECM) to maintain its stability with broad uptake and storage of vitamin A, and also regulate liver regeneration. But in pathological conditions, HSCs are activated by constant stimulations or liver injury, then with activated proliferation, reduced lipid drops, and increased ECM synthesis. Morphology of these cells also changes from the star-shaped stellate cells to that of fibroblasts or myofibroblasts with obvious contractibility and secretion of cytokines and chemokines including a variety of proinflammatory factors and adhesion molecules, suggesting that the activation of HSCs is one of the key events in the development of liver fibrosis. Study on the isolation and function of HSCs is always one of the hot topics for liver biology. In this review, we systematically summarize and discuss the recent advances in our understanding of the isolation methods and improvements of HSCs, and functional research of HSCs biology in health and disease, as well as potential directions.
Extracellular Matrix ; metabolism ; Hepatic Stellate Cells ; cytology ; Humans ; Liver ; cytology ; Regeneration ; Vitamin A ; metabolism

Objective To study the effect of ATP sensitive potassium channel(KATP)opener on neuronal apoptosis and the expression of caspase-8 mRNA and protein in rats following cerebral ischemia-reperfusion.Methods 200 male Wistar rats were randomly divided into sham-operated group,control group,KATP opener precondition group(KATP opener group)and KATP opener and blocker precondition group(KATP blocker group).The middle cerebral artery ischemia-reperfusion models were established by using the intraluminal suture occlusion method.Neuronal apoptosis were detected by TUNEL staining,the expression of caspase-8 mRNA and protein were detected by immunohistochemical staining and RT-PCR methods.Results(1)The numbers of apoptotic cells in KATP opener group at 12 h,24 h,48 h,72 h after cerebral ischemia-reperfusion were significantly less than those in control group and KATP blocker group(P

Objective To investigate the relationship of the classification of Doppler ultrasound blood flow signals with matrix metalloprotei-nase-9 (MMP-9)expression and microvessel density (MVD)in primary hepatocellular carcinoma (PHC).Methods Sixty patients with PHC,who underwent surgical resection in the Second Affiliated Hospital of Dalian Medical University from April 2008 to April 2013, were included in the study.These patients underwent ultrasound examination before operation.The classification of Doppler ultrasound blood flow signals in the focus was recorded.The expression of MMP-9 and CD3 1 in carcinoma tissues was detected by immunohistochemistry, and MVD was calculated.Continuous data were analyzed by t test,while categorical data by chi-square test;the relationship was evaluated by Spearman correlation analysis.Results The classification of Doppler ultrasound blood flow signals was as follows:grade 0 (15 cases);grade 1 (20 cases);grade 2 (15 cases);grade 3 (10 cases).The positive rate of MMP-9 expression was 72.3%.The MVD was 43.2 ± 5.4.The classification of Doppler ultrasound blood flow signals in the focus was positively correlated with MMP-9 expression and MVD (r=0.56,P<0.05).Conclusion The classification of Doppler ultrasound blood flow signals can reveal vascular changes in the focus, and it can be used in the clinical evaluation of angiogenesis in tumor tissues and the clinical diagnosis and treatment of PHC.

Hospital-acquired conditions (HACs)are key to patient safety.This paper introduced a new classification system of HACs,the CHADx model,and described the principle and method of establishing the model as well as the problems found during its use.The model can provide a basis for studying the problem of incidence,causes and influence factors of hospital acquired conditions,so as to help medical institutions to explore the causes of HACs and to ensure patient safety and improve the quality of health care.

Objective To evaluate the diagnostic value of multislice CT(MSCT)in multilocular cystic real cell carcinoma(MCRCC).Methods Imaging features of 22 patients with MCRCC proved by histopathologic examinations,these patients were underwent non-enhanced and dual-phase contrast-enhanced CT scans and evaluated CT signs.Results 22 tumors all appeared as well defined multilocular cystic mass.The cystic wall presented with irregular thicking in 14 cases and regular thicking in 8 cases.The tumors with thin coarse septum in 6 cases(the transverse diameter ≤2 mm),irregular thicking septum in 11cases(2mm ＜the transverse diameter ≤ 5 mm)and septum with nodule in 5 cases(the transverse diameter ≤ 5 mm).Cystic wall and septum enhancement were detected with dual-phase contrast-enhanced CT.Cystic ares and hemorrhagic areas and calcification were no enhanced.Calcification of cystic wall were found in 2 cases and cyst in 3 cases.Conclusion MSCT imaging appearances have typical features.It has great important value in the preoperative diagnosis of MCRCC with non-enhanced and dual-phase contrast-enhanced CT scans.

Objective To describe the mastery of knowledge about antihypertensive drugs of clinical nurses in one tertiary comprehensive hospital of Beijing.Methods Purposive sampling was used to recruit 195 clinical nurses who were working in one tertiary comprehensive hospital in Beijing.The demographic questionnaire,knowledge of antihypertensive drugs questionnaire were filled.Results The score of knowledge of antihypertensive drugs Questionnaire was (45.63 ± 3.79) points;different education,wishes to take part in hypertension related knowledge training had significant differences (t =2.007,2.049,P ＜ 0.05).Conclusion Knowledge of antihypertensive drugs of clinical nurses in Beijing was at medium level.Those who indicated that it was not matter whether to attend hypertension related knowledge training and had low level of education had more problems in knowledge of antihypertensive drugs.

Objective To investigate the clinical efficacy and summarize the experience of treating patients with hepatic injuries in a primary-care hospital.Methods The clinical data of 230 patients with hepatic injuries from January 2002 to December 2014 were retrospectively analyzed.Results 124 patients were treated with surgery and 106 patients (including two patients who were transferred to another hospital) were managed by conservative treatment.Of 230 patients,204 patients recovered well and 26 patients died.63 cases of severe hepatic injuries (grades Ⅲ,Ⅳ,Ⅴ and Ⅵ),the mortality rate reached 25.4％.Concltsions The important points to success in managing these patients are early diagnosis and adequate assessment of liver injury,proper choice of treatment and using the simplest and the most effective means in surgery to control bleeding.

Objective To study the mechanism of enhancing effect of pretreatment with Geqin Tablets (GT). Methods The rat preconditioning models of myocardial ischemia were established by an improved pushing tube method. Cell apoptosis was determined with TUNEL. The expression of PKC was examined by immunohistochemical method. The serum SOD activity was tested by hydroxylamine hydrochloride method and MDA activity by TBA method. Results The amount of apoptotic cells increased in ischemia /reperfusion (IR) group significantly(P

Objective To determine the efficacy of lifestyle interventions in adults with impaired glucose tolerance (IGT).Methods MEDLINE (Pub Med),EMBASE,Science Citation Index (SCI),and Cochrane Database were retrieved for articles about the relations of lifestyle intervention and IGT.Searches were limited to English language publications.The RCTs outcome evaluated in this study included 2 h plasma glucose level and fasting plasma glucose,meta-analysis was carried oat by Stata 11.0 among articles for the inclusion and exclusion criteria.The difference of effects was expressed as standardized mean deviation(SMD).Results A total of 10 RCTs studies met the inclusion criteria.Lifestyle interventions were associated with a decline in 2 h plasma glucose and fasting plasma glucose level (SMD =-0.67,P＜0.01 ;SMD =-0.33,P＜0.01),but high heterogeneity was identified in this meta-analysis.Conclusion Physical,dietary and both combined interventions can reduce 2-h plasma glucose and fasting plasma glucose levels in adults with impaired glucose tolerance.As high heterogeneity was identified in this meta-analysis,more high quality research is needed.

Objective To explore the effect of proteasome inhibitor bortezomib (BOR) on proliferation inhibition and apoptosis in imatinib-resistant chronic myeloid leukemia cell line K562/G01. Methods MTT assay was used to observe the effect of growth inhibitory of cells; flow cytometry was used to detect cell cycle and apoptosis. Results K562/G01 cells was not sensitive to imatinib,1C50 values of imatinib to K562 and K562/G01 cells was (20.09±1.38) and (0.54±0.13) μmol/L,respectively; BOR could inhibit proliferation in K562/G01 cell,peaked at 48 h,and IC50 value of BOR to K562/G01 was (23.10±2.71) nmol/L. G2/M phase cell cycle arrest and significant apoptosis peak could be seen by flow cytometry with increased in the concentration and action time of BOR. Conclusion BOR can inhibit proliferation and induce apoptosis in imatinib-resistant K562/G01 cell,the mechanism may be related to cell cycle G2/M phase arrest.