Objective To explore the expression of the Pendrin gene (SLC26A4) and protein in multinodular goiter.Methods Thyroid tissues were obtained from 40 multinodular goiter patients undergoing surgery while the control group were obtained from 40 nomal thyroid tissues.RT-PCR was used to test SLC26A4 gene while western blot and immunohistochemistry were used to test Pendrin protein expression and distribution.Results SLC26A4 mRNA expression in multinodular goiter tissue was significantly increased in comparison with normal nodular tissues (t=2.663,P=0.011).Pendrin protein expression in multinodular goiter group was higher than that in normal tissue (t=2.286,P=0.026).The immunohistochemistry results showed that the Pendrin protein in multinodular goiter was mainly located in cytoplasm.There was positive expression in 24 patients (60％) in multinodular goiter group,while it was in 14 patients (35％) in the normal control group.The difference was significant (X2=5.013,P=0.025).Pendrin protein mainly expressed in cytoplasm in multinodular goiter tissue while it was mainly in cytomembrane in the normal control group.Conclusion SLC26A4 mRNA and its coding protein Pendrin expression are increased in multinodular goiter group,and mainly located in cytoplasm,indicating that iodide transporter function may be damaged when multinodular goiter occurs.

Wether cancer patients can re-adapt to life or not after experiencing tremendous frustration is the focus of tumor care nowadays.Since the measurement methods and outcome indexes of cancer patients adaptation level are different,adaptation related factors and predictors are controversial and there is not yet a unified opinion.Currently,it is thought that demographic information (like age,gender,education),disease-related information (like physiological indicators,treatment options,body image and sex) are closely related to cancer patients adaptation after stress.However there are huge differences among the extents and effects.

Objective To study the clinical value of three-dimensional vascular reconstruction of power Doppler imaging in monitering acute rejection of renal transplant.Methods Eighty-eight renal allograft patients were studied by three-dimensional vascular reconstruction and graded the vascular flow.Results The ratio of successful renal transplant three-dimensional vascular reconstruction reached 95.5% in 88 cases.Blood flow gradation was 4-5 levels in 30 controls and 0-4 levels in 58 patients with acute renal transplant rejection. Blood flow gradation was closely relevant to the time when the blood flow began to improve and the renal function began to restore after treatment, value of r being -0.9224 and -0.9223 respectively (P

Objective To explore aescin's inhibition of in vitro proliferatim in L1210 murine acute lymphoid leukemia cells and its in vivo antileukemic effects.Methods MTT assay was used to determine the anti-proliferative effect in vitro;Leukemia transplant models were used to test the antileukemic effect. Mice inoculated with L1210 cells were treated with ten daily doses of aescin or/and each other day of DOX, and were observed for survival.Antileukemic effects were assessed by calculating the extension of lifespan. Results Aescin(20～60?g/mL) was found to be able to inhibit the proliferation of L1210 cells.The effects were in a dose- and time- dependent manner.The IC_(50) value of aescin in L1210 cells after 72 h was (24.86?2.23)?g/mL.In vivo study showed that after treating the L1210 cells bearing mice with higher, middle,and low dosage(4.5,3.5,and 2.5 mg/kg) of aescin for ten consecutive days,The extension of lifespan were 25.8%(P

OBJECTIVE:To provide reference for local colleges to cultivate innovative ability of applied talents for pharmacy major.METHODS:With innovative thinking,innovative methods and innovative practice ability training as the starting points,the cultivation of students' innovative ability permeated all aspects of the teaching process mainly through optimizing the curriculum system,deepening the teaching reform,strengthening the project-driven and expanding the practice platform construction.RESULTS:The TMP trinity pharmacy professional applied talents innovation ability training system was constructed through adjusting curriculum system and credit ratio,strengthening the setting of humanities course,establishing professional core,professional refinement and professional extension courses,increasing the proportion of elective course,deepening the teaching reform from four aspects of teaching,learning,evaluation and construction;encouraging students to compete;establishing three phase-four module multidimensional practice teaching platform.CONCLUSIONS:Established system has realized the stereoscopic creation of students' innovative thinking,diversified methods of innovation and multi-levels of innovation and practice.Students have got a coordinated development in the quality,knowledge,ability.It is helpful to improve the quality of talents cultivation in local colleges.

Objective To study PTEN protein expression and clinical significance in patients with diffuse large B cell lymphoma. Methods Immunohistochemical staining was used to determine the PTEN protein expression in 40 cases of primary diffuse large B lymphoma tissuse. The results were analyzed by Kaplan-Merie survival analysis, Log-Rank test and Logistic regression analysis. Results PTEN protein was positive in 16 cases and negative in 24 cases. There was no significant difference between two groups in twoyear overall survival rate(62.5 % vs 66.7 %, P >0.05). Survival analysis showed that patient' s survival time gradually were reduced with extended time between PTEN protein-positive group and negative group, lower in PTEN-positive group than the negative group, but there was no significant difference in survival curve (P >0.05) in the two groups. We compared characteristics of patients between PTEN protein positive and negative groups,including molecular type, patient' s age, stage, LDH, physical score and extranodular invasion, there was no significant difference among them. PTEN protein was not correlated with prognosis, while International Prognosis Index(IPI) was still a risk factor (OR >1). Conclusion PTEN protein expression may not predict the outcome in diffuse large B cell lymphoma, but IPI still is a predictor.

Objective To explore the prognostic significance of p53 mutation protein in patients with diffuse large B-cell lymphoma for the purpose of individualized therapy. Methods Newly diagnosed 62 cases were randomly chosen from our hospital, p53 mutation protein and CD10, bcl-6, MUM1 were tested by immunohistochemistry. Correlation of p53 mutation protein with patients ' characteristics, genotype and survival were analysed in the study. Results p53 mutation protein was found in 48.4 % (30/62) of patients.Its expression was only related to initial treatment response (x2 =20.365, P =0.040), including complete remission rate of 33.3 % (10/30) in positive group and 59.4 % (19/32) in negative group, and non-germinal center genotype (x2=31.023, P =0.021) with 83.3 % in positive group and 56.2 % in negative group. No other correlation was not verified with clinical features. Multivariate survival analysis showed that p53 mutation protein was an independent predictor for shorter progress-free and overall survival in positive group (x2 =30.784, P =0.005 and x2 =35.276, P =0.006). Conclusion p53 mutation protein should be an independent predictor with poor prognosis and to direct personalized therapy.

Objective To study the clinical characteristics, diagnosis, treatment and prognosis of primary pulmonary peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS). Methods Two cases of primary pulmonary PTCL-NOS were studied and relevant literature were reviewed. Results Case 1 diagnosed as primary pulmonary PTCL-NOS was a 44 years old woman and disease progressed after GLD (Gem+L-OHP+DXM) chemotherapy regimen. At last, the patient died of respiratory failure after one month. Case 2 diagnosed as primary pulmonary PTCL-NOS was a 46 years old man and reach partial response after CHOP regimen, and still alive now.Conclusion Primary pulmonary PTCL-NOS is very rare. It is easy to be misdiagnosed due to non-specific clinical and imaging manifestations. Acquiring enough tissue specimens for pathologic examination is the key to a definitive diagnosis. At present, there is no standard chemotherapy regimen for these patients, the prognosis is relatively poor.

Objective:To evaluate the efficacy and safety of sequential platinum regimen in young patients with diffuse large B-cell lym-phoma (DLBCL). Methods:Newly diagnosed young patients with DLBCL, who were hospitalized from January 2005 to June 2012 in the Affiliated Cancer Hospital of Zhengzhou University, were selected according to the requirements. The patients were divided into stan-dard and sequential platinum regimen groups. The remission rates were compared usingχ2 test, whereas the five-year survival rates between the two groups were compared using the Kaplan–Meier method. Multivariate survival analysis was performed using the Cox proportional regression. Subgroup analysis was conducted to select candidate patients for the sequential platinum regimen. Results:A total of 331 patients were enrolled in the study, in which 129 were provided with sequential platinum regimen and 202 were provided with the standard regimen. Sequential regimen yielded higher rates of complete remission (80%vs. 63%, P=0.001), five-year progres-sion-free survival (PFS;60%vs. 50%, P=0.014), and overall survival (OS;70%vs. 58%, P=0.016) than the standard regimen. Multivariate analysis revealed that sequential regimen was an independent prognostic factor for PFS (hazard ratio HR=0.635, P=0.012) and OS (HR=0.625, P=0.021). Subgroup analysis showed that patients with good prognosis and patients who did not receive rituximab benefited more from the sequential platinum regimen. Sequential platinum regimen did not increase the occurrence of adverse effects com-pared with the standard regimen. Conclusion:Sequential platinum regimen is a safe treatment that can improve the survival of young patients with DLBCL. Patients with good prognosis and patients who did not receive rituximab can benefit more from the treatment with sequential platinum regimen.

Objective To explore the impacts of short-term continuous subcutaneous insulin infusion (CSII) therapy on insulin secretion and sensitivity in newly-diagnosed type 2 diabetes. Methods Ten patients(7 males, 3 females) with newly-diagnosed type 2 diabetes accepted a 2-week course of intensive CSII therapy during June 2006 and February 2007. Hyperinsulinemia euglycemia clamp test and intravenous glucose tolerance test (IVGTT) were performed before and after CSII. Results (1) None of the patients had acute insulin response (AIR) before CSII treatment, and most of them had a partial restoration after 2-week intensive therapy. The AIR after CSII was significantly increased [(7.63±4.73 vs 0.83±1.96)mU/L, P＜0.01]. The patients whose AIR restored better tend to be younger and obeser. (2) The glucose infusion rate (GIR) before CSII in the newly-diagnosed type 2 diabetic patients was significantly lower than that in healthy controls [(2.30±0.81 vs after 2-week CSII therapy (P＜0.01). The patients whose GIR increased greatly had significantly lower waist circumferences and body mass index, and higher basal blood glucose levels. Conclusion Normalization of blood glucose levels by transient intensive CSII therapy improves pancreas β-cell function and insulin sensitivity, which seems to contribute to the long-term diabetic remission.