OBJECTIVE To investigate the resistance of Pseudomonas aeruginosa strains to 5 ?-lactams.METHODS Susceptibility of 218 P.aeruginosa strains isolated from Anhui Province in 2005 to ceftriaxone,cefotaxime,ceftazidime,aztreonam,and piperacillin were tested by agar dilution method.RESULTS The resistance rate to ceftriaxone,cefotaxime,aztreonam,piperacillin,and ceftazidime were 59.2%,57.3%, 56.0%,51.8%,and 39.0%,respectively.There was statistically significant difference between ceftazidime and other 4 ?-lactams(P0.05).CONCLUSIONS Ceftazidime is the most susceptible antibiotic to P.aeruginosa strains among 5 ?-lactams tested.

OBJECTIVE To analyze the drug resistance of 141 clinical strains of Acinetobacter baumannii to 15 kinds of antimicrobial agents for the rational application of antimicrobial agents.METHODS All 141 strains of A.baumannii isolates were tested for minimal inhibitory concentration(MIC) to 15 kinds of antimicrobial agents included meropenem,imipenem,piperacillin,and so on using agar dilution method.RESULTS The lowest resistance rate of A.baumannii to the antimicrobial agents was to imipenem(22.7%),then was to meropenem and cefoperazone/sulbactam.The highest resistance rate was tetracycline(81.5%).The resistance rate to other antimicrobial agents was to about or more than 50%.CONCLUSIONS Most of A.baumannii isolates are susceptible to meropenem,imipenem,and cefoperazone/sulbactam and resistant to other antimicrobial agents,therefore,we should enhance to this kind of isolates detection.

OBJECTIVE To investigate the resistance and molecular epidemiology of Pseudomonas aeruginosa isolated from ICU. METHODS Susceptibility of P. aeruginosa isolates to 14 antimicrobial agents were tested by microScan WalkAway-40 system,and Molecular typing was analyzed by enterobacterial repetitive intergenic consensus(ERIC-PCR). RESULTS The lowest resistance rate of P. aeruginosa to the antimicrobial agents was to ceftazidime (27.3%),then to cefepime (45.5%). The highest resistance rate was to cefotaxime (100.0%) and the resistance rate to other antimicrobial agents was more than 60.0%. Patterns of ERIC-PCR were same in 5 strains of P. aeruginosa. CONCLUSIONS P. aeruginosa isolated from ICU is resistant to most of antimicrobial agents and clone spread is found in them. We should enhance detection and surveying of these isolates.

Objective To investigate the effect of informational support on disease uncertainty in liver cancer patients undergoing interventional therapy. Methods Sixty patients of liver cancer patients undergoing interventional therapy were randomly divided into the intervention group and the control group with 30 in each.The control group was treated with general treatment and care. The patients in the intervention group were given general treatment and care with a series of informational support interventions. Patients in the two groups were measured by uncertainty in illness scale (MUIS) at the day of admission,before operations and discharged from hospital. The measurement results were statistically analyzed. Results There was significant decrease about disease uncertainty scores both on the day before operation and the day before discharge in the intervention group compared to the day of admission. The decreasing score of uncertainty in illness in the intervention group was significantly greater than the control group on the day before operation and the day before discharge. Conclusions A series of informational support interventions could significantly reduce the diseases uncertainty in liver cancer patients undergoing interventional therapy.

Objective To investigate the adhesion levels in uropathogenic Escherichia coli with various degree of drug resistance.Methods One hundred strains of Escherichia coli isolated from urine specimen were collected from patients admitted to 4 Grade A tertiary hospitals in Tianjin during March 2012 to October 2015.Escherichia coli were divided into drug sensitive group and drug resistant group by drug sensitivity tests with 50 strains in each group.The expressions of fimH,fimA,fimB genes of type I fimbriae and papA,papB,papC,papGII genes of P fimbriae were detected by polymerase chain reaction(PCR)and real-time fluorescence quantitative RCR (RT-PCR),respectively.Adhesion ability of type I fimbriae and P fimbriae were tested by yeast cell adhesion test and erythrocyte agglutination test.Chi square test and t(Z) test were used to analyze the data.Results The positive rate of papGII in drug resistant group (42.0%) was significantly higher than that in the drug sensitive group (16.0%)(χ2 =8.208,P <0.05),while there were no significant differences in the positive rates of fimH,fimA,fimB,papA,papB and papC genes between two groups(all P >0.05).The expression levels of fimH,fimB and papC genes in the sensitive group were higher than those in the resistant group(Z =3.427,t =5.182 and 8.120,all P <0.05).The adhesion ability of strains carrying type I fimbriae in sensitive group was stronger than that of resistant group (χ2 =5.769,P <0.05).Conclusions The decline in adhesion ability of type I fimbriae in drug resistant E.coli strains is possibly associated with the adaptive cost of bacteria,the transcription and deficiency of other genes encoded by fim and pap gene cluster will also affect the adhesion function of type I pili and type P pili.

Objective To assess the value of CT in identification and diagnosis of benign and malignant calcified thyroid nodules.Methods Retrospective analysis was performed on the CT data of 313 surgically and pathologically confirmed cases with 378 calcified nodules.Based on the size,morphology,and number,calcification was divided into microcalcification (d≤2 mm and axis displayed in only one cross-section),coarse calcification (d＞2 mm or displayed in two or more cross-sections),annular calcification (arc or annular),and multiple microcalcifications (solitary multiple microcalcification without a soft tissue lump);a distribution of microcalcification,coarse calcification,and annular calcification as well as a clearer enhanced periphery or internal calcification than nonenhanced data in benign and malignant thyroid nodules were observed.Results The 378 nodules consisted of 259 benign nodules (68.5％) (all were nodular goiters) and 119 malignant nodules (31.5％) (including 111 papillary thyroid carcinomas,4 follicular carcinomas,3 medullary thyroid carcinomas and 1 lymphoma).Microcalcification was more common in malignant nodules (MNs) than in benign nodules (BNs),with a rate of 43.6％ vs 12.4％,respectively (P≤0.05),and its sensitivity,specificity,positive predicted value,and negative predicted value were 42.9％,87.6％,61.4％ and 76.9％,respectively.Coarse calcification,annular calcification,and clearer enhanced periphery or internal calcification than nonenhanced data were more common in BNs than in MNs,with rates of 52.9％ vs 20.2％ (P≤0.05),66.0％ vs 42.0％ (P≤0.05) and 43.2％ vs 19.3％ (P≤0.05),respectively,whose sensitivity,specificity,positive predicted value and negative predicted value were 66.0％ vs 22.4％ vs 43.2％,58.0％ vs 86.6％ vs 80.7％,77.4％ vs 78.4％ vs 83％,and 43.9％ vs 33.9％ vs 39.8％,respectively.Two multiple microcalcifications without a soft tissue lump were MNs (papillary thyroid carcinoma).Conclusions Microcalcification and multiple calcifications are conducive to the diagnosis of MNs,whereas coarse calcification,annular calcification,and clearer enhanced periphery or internal calcification than nonenhanced data benefit the diagnosis of BNs,but the low specificity and high false positive rate suggest that the judgment of BNs or MNs should not depend on coarse calcification alone.

Objective The ‘Spider Track Map (Fuwai)’is an innovative tool for comprehensive evaluation of scientific research performance.It was developed jointly by the Science & Research de partment and Medical Research & Biometrics Center in Fuwai Hospital.This tool aims to evaluate the development of scientific performances in 3 consecutive years of targeted departments,which will be considered to assess the technological competitiveness of each department.Methods The key variables were the number of projects,funding,awards,publications and patents during the last three years.After standardization,the weighted sum of those variables was calculated.Then the ‘Spider Track Map (Fuwai) ’ could be generated and the evaluation conclusion would be obtained after stratification.Results The ‘Spider Track Map (Fuwai)’ method had been used to evaluate the scientific research performance in 2015.The excellent research departments had been successfully elected and rewarded.Conclusions Beside the excellent department election,the method can also identify different feature of performance including improved,retrogressive,high efficiency or no changes.This multidimensional tool provides the reference to administrators for scientific commendation and reward and also the guid ing and alerting for specific department.

OBJECTIVE To investigate the resistance to ?-lactam antibiotics and meropenem of non-ESBLs-producing Escherichia coli isolates.METHODS Non-ESBLs producers of E.coli isolates were detected by CLSI phenotypic confirmatory test,and susceptibility of these isolates to ?-lactam antibiotics and meropenem was tested by agar dilution method.RESULTS The resistance rate of these isolates to ampicillin,cefazolin and cefoxime was more than 60% and that to cefoperazone-sulbactam,cefmetazole and cefepime was 19.2%,23.8% and 31.8%,respectively,but most were susceptible to cefotaxime,ceftazidime,and meropenem.CONCLUSIONS The most isolates of non-ESBLs-producing E.coli are resistant to ampicillin and to the first and second generation cephalosporins,but they are susceptible to meropenem and the third generation cephalosporins.

OBJECTIVE To investigate the resistance in clinical isolates of Stenotrophomonas maltophilia to antimicrobial agents. METHODS Susceptibility of 48 S. maltophilia isolates were tested by agar dilution or K-B method. RESULTS The lowest resistance rate of isolates to the antimicrobial agents was to ticarcillin/clavulanic acid (12.5%),then to minocycline (25.0%) and trimethoprim/sulfamethoxazole (31.3%). The resistance rate to ceftazidime,ceftazidime/sulbactam,levofloxacin,and chloramphenicol was 62.5%,52.1%,52.1%,and 50.0%,respectively. CONCLUSIONS Most of S. maltophilia isolates are susceptible to ticarcillin/clavulanic acid,minocycline,trimethoprim/sulfamethoxazole and resistant to other antimicrobial agents. More mornitoring should be enhanced.

Objective To study the killing effects of fractional proteins from Microtus fortis (Mf) serum on schistosomula of Schistosoma japonicum. Methods Mf serum proteins were separated into albumin and globulin by means of salt out of ammonium sulfamate. The globulin was then separated into 4 big and 12 small fractional proteins through Sephacryl S-300 column chromatography and electrophoresis elution. The killing effects were observed in vitro in cultivation in which the purified fractional proteins and schistosomula of S. japonicum were incubated together for 48 h. Results The mortality rate of schistosomula acted by Mf globulin was 59.2% and when added with complements was 68.4%. The killing effects of the 2nd and 3rd big fractional proteins were the same as that of Mf globulin. Three small fractional proteins (3.2, 3.3, 3.4) showed the higher killing effects which made the mortality rate of schistosomula 45.1%, 57.6% and 67.2%, respectively. The fractional protein of 100-135 kDa also showed the same killing effect as that of Mf globulin. Globulin from BALB/c mice sera had no significant effect on schistosomula. There was no significant difference in the mortality rate of schistosomula acted by both albumins. Conclusions Mf globulin has significant killing effects on schistosomula of S.japonicum in vitro and 100-135 kDa fractional protein may be an important effective molecule.