OBJECTIVE:To study the effect of different administration time of ibuprofen on the changing characteristics of circadian rhythm about body temperature and plasma concentration in rats.METHODS:60 rats were given single oral administration of ibuprofen at the dose of 50 mg per kilogram at different time(2∶00,6∶00,10∶00,14∶00,18∶00,22∶00) according to a randomized block design with another 10 rats serving as blank control.The effects of ibuprofen on circadian rhythm variations of body temperature and plasma concentration in rats were observed and analyzed using chronogram and cosine method.RESULTS:The body temperature of rats prior to and after administration both showed a significant circadian rhythm,with mean declined only,yet acrophase and amplitude showed no significant differences.The best cosinor curve equation were Yt i=38.7+0.52 Cos(15??ti—86.80?) and Yt i=37.8+0.55 Cos(15??ti—117?) respectively.The plasma concentration also showed a significant circadian change in rats at the different time points of administration.The plasma concentration in light phase was higher than that in dark phase,and the best cosinor curve equation was Yt i=41.95+5.30 Cos(15??ti—272.83?).CONCLUSION:The differences of circadian rhythm were both shown about body temperature and plasma concentration in rats when ibuprofen was orally administered at the different time points.

Objective To investigate the effect of pregnancy management of gestational diabetes mellitus (GDM) on pregnancy outcome.Methods One hundred and two patients of GDM was as our case group and 100 patients with non-diabetic and no medical complications in pregnant women in the same period were as control group who all hospitalized from January 2008 to December 2011.The information of maternal and prenatal complications were collected.Results Pregnancy women were underwent the pregnancy management.No significant difference was seen in terms of the incidences of pregnancy induced hypertension polyhydramnios,preterm delivery,premature rupture of membranes and postpartum hemorrhage between case group and control group(x2 =1.33,0.80,0.68,0.35,0.20,respectively,P ＞ 0.05),so were other indices including of the incidences of fetal macrosomia,fetal distress,neonatal asphyxia,hyperbilirubinemia and neonatal hypoglycemia between the two groups(x2 =0.09,0.23,0.15,0.15,0.49,respectively,P ＞0.05).Conclusion Enhancing the management and standardized treatment of GDM can control the level of serum glucose and decrease the maternal and prenatal complications.

Objective: To observe the histomorphology/pathology changes and the expression of hypoxia inducible factor-1 alpha (HIF-1?) and vascular endothelial growth factor (VEGF) in drowning rats brain, in an attempt to explore new pathways for treatment of water drowning. Methods: The animal model of freshwater drowning was established with rats. The rats were randomly divided into the following groups: control group, drowning-to-death group and 2 min-drowning group (including 5 subgroups survived for 3, 6, 12, 24. and 40 hours after being drowned in water for 2 min [n=10]). Brain indices of rats in each group were detected and the pathological changes of cerebral tissues were observed; the expression of HIF-lo and VEGF were detected immunohistochemically and the correlation between them was analyzed. The dynamic blood oxygen saturation was detected in rats survived for 40 h after 2 min-drowning at different points. Results: Brain index of the drowning group and groups surviving for 24, 40 hours after drowning were obviously higher than that of the control group (P

CD+4 CD+25 regulatory T cell (Treg) has immune incompetent and immune suppression functions, and is a kind of suppressor T-cell subsets. It can inhibit the activation and proliferation of CD+4 T cells and CD+8 T cells, so as to effectively suppress the immune system to foreign organ and reduce the graftversus-host disease (GVHD) after hematopoietic stem cell transplantation (HSCT), without affecting the role of graft-versus-leukemia (GVL), which plays an important role in the transplantation immune tolerance.

Objective To investigate the relationship of the classification of Doppler ultrasound blood flow signals with matrix metalloprotei-nase-9 (MMP-9)expression and microvessel density (MVD)in primary hepatocellular carcinoma (PHC).Methods Sixty patients with PHC,who underwent surgical resection in the Second Affiliated Hospital of Dalian Medical University from April 2008 to April 2013, were included in the study.These patients underwent ultrasound examination before operation.The classification of Doppler ultrasound blood flow signals in the focus was recorded.The expression of MMP-9 and CD3 1 in carcinoma tissues was detected by immunohistochemistry, and MVD was calculated.Continuous data were analyzed by t test,while categorical data by chi-square test;the relationship was evaluated by Spearman correlation analysis.Results The classification of Doppler ultrasound blood flow signals was as follows:grade 0 (15 cases);grade 1 (20 cases);grade 2 (15 cases);grade 3 (10 cases).The positive rate of MMP-9 expression was 72.3%.The MVD was 43.2 ± 5.4.The classification of Doppler ultrasound blood flow signals in the focus was positively correlated with MMP-9 expression and MVD (r=0.56,P<0.05).Conclusion The classification of Doppler ultrasound blood flow signals can reveal vascular changes in the focus, and it can be used in the clinical evaluation of angiogenesis in tumor tissues and the clinical diagnosis and treatment of PHC.

Objective To investigate the effect of clinical pathway on patients with tubal pregnancy under-went laparoscopic treatment .Methods 150 patients with tubal pregnancy undergoing laparoscopic treatment were randomly divided into pathway group and control group , 75 patients in each .The patients in the pathway group were given clinical pathway management , whilepatients in the control group were given conventional management .The in-dexes, such as patients'average hospitalization time, average treatment cost, satisfaction, and the rate of health knowl-edge awareness of the two groups were compared .Results The average hospitalization time and the average treat-ment costof the pathway group were significantly shorter and lower than those of the control group , with statisticalsig-nificance (p<0.05), and the rate of satisfaction and health knowledge awareness of the pathway group were signifi -cantly higher than those of the control group (p<0.05).Conclusion The clinical pathway in patients with tubal pregnancy treated with laparoscopyis worth to popularize in clinical application , because it may effectively shorten the hospitalization time , reduce the treatment cost and improve the rate of satisfaction and health knowledge awareness .

Objective To investigate the efficacy of recombinant human erythropoietin(rHuEPO) in treating the anemia in obstrics. Methods 40 antepartum anemia patients and 36 postpartum anemia patients were treated with rHuEPO 8000IU intravenously. Another 40 antepartum and 36 postpartum anemia patients were selected as control. Oral supplementation of iron and folic acid were used both in study and control group. Results The hemoglobin level was increased significantly in the study group than control group both in the prenatal and postpartum anemia patients. ( P0.05 )。 Conclusion rHuEPO therapy for obstetric anemia is effective and safe.

Objective To evaluate the effect of ZISHENYUTAIWAN in the treatment of threatened abortion. Methods From March to September 2000, ZISHENYUTAIWAN was used to treat 221 cases of early threatened abortion and 10 cases of late threatened abortion in 8 hospitals in Beijing. Results Effective rate of early threatened abortion is 91.89%. Conclusions ZISHENYUTAIWAN is effective and with less side effect for early threatened abortion.

Objective: To evaluate the polysomnology results along with mandibular titrated advancement using oral appliance to treat obstructive sleep apnea and hyponea syndrome(OSAHS).Methods: Several electronic databases (PubMed, Embase, Cochrane Library, CNKI, VIP, and Wanfang) were systematically searched up to September 2015.There was no restriction of language or source of information.All randomized clinical trials (RCT) and before-after trials (BAT) comparing at least two different mandibular advancements were included.Two independent reviewers selected the studies, extracted data and evaluated risk of bias by quality assessment.Data were pooled using a fixed-effects model, and the summary effect measure was calculated by risk ratio (RR) and 95%CI.Meta-analysis was performed using RevMan 5.3 software.Results: Two RCTs and five BATs were included in the review.Among the five BATs, two of them were randomized, while the other three were not.Outcomes including apnea hypopnea index (AHI), oxygen desaturation index (ODI), success rate (reduction of AHI or ODI >50%), normalization rate (AHI or ODI<10/h) were assessed in this review.Based on the trial design and quality assessment, four studies were included for meta-analysis.No significant difference in the success rate was found between the group with 50% of the maximal mandibular advancement (MMA) and the group with 75% of MMA [I2=0%, RR=0.93, with 95%CI (0.80, 1.09)].No significant dif-ference in the normalization rate was found between the 50% of MMA and 75% of MMA groups [I2=45%, RR=0.85, with 95%CI (0.68, 1.06)].Subgroup analysis displayed that the severity of OSAHS before treatment was a potential factor affecting the normalization rate.Conclusion: Based on current available evidence, the success rate and normalization rate for treating OSAHS in the patients with 75% MMA were not found to be significantly higher than those with 50% MMA.Due to small simple size in this meta-analysis, the results of the present study should be interpreted with caution.Further prospective studies are needed to strengthen the evidence.

Objective To study the effect of ATP sensitive potassium channel(KATP)opener on neuronal apoptosis and the expression of caspase-8 mRNA and protein in rats following cerebral ischemia-reperfusion.Methods 200 male Wistar rats were randomly divided into sham-operated group,control group,KATP opener precondition group(KATP opener group)and KATP opener and blocker precondition group(KATP blocker group).The middle cerebral artery ischemia-reperfusion models were established by using the intraluminal suture occlusion method.Neuronal apoptosis were detected by TUNEL staining,the expression of caspase-8 mRNA and protein were detected by immunohistochemical staining and RT-PCR methods.Results(1)The numbers of apoptotic cells in KATP opener group at 12 h,24 h,48 h,72 h after cerebral ischemia-reperfusion were significantly less than those in control group and KATP blocker group(P