OBJECTIVE:To evaluate the application effects of PDCA cycle management in the outpatient prescription intervention.METHODS:A total of outpatient prescriptions (pre-intervention group) during Jan.-Dec.2014 and 918 659 (intervention group) were selected during Jan.-Dec.2015 were selected from a hospital.Irrational 9utpatient prescriptions were analyzed before and after the application of PDCA cycle management,and the improvement of main indexes were compared such as unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample.RE SULTS:Before intervention,there were 2 347 irrational prescriptions,including 1 401 nonstandard prescriptions,849 unsuitable prescriptions and 97 abnormal prescriptions.After intervention,there were 1 161 irrational prescriptions,including 695 nonstandard prescriptions,425 unsuitable prescription and 41 abnormal prescriptions,decreasing 50.53％,50.39％ 49.94％ and 57.73％,respectively.The proportion of unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample,nonstandard prescription paper,unsuitable indications,no signature and date for prescription revision or no reason and new signature for overdose use,outpatient prescriptions more than 7 d common dose or emergency prescriptions more than 3 d common dose without special situation in total amount of outpatient prescriptions decreased from 0.72‰,0.57‰,0.45‰,0.27‰,0.20‰,0.19‰ and 0.15‰ before intervention to 0.32‰,0.25‰,0.19‰,0.11‰,0.09‰,0.08‰ and 0.07‰ after intervention.CONCLUSIONS:PDCA cycle management significantly improves the quality of outpatient prescriptions.There still are irrational outpatient prescriptions in this hospital,and it is to be intervened continuously.

Objective To evaluate the clinical efficacy of cattle encephalon glycoside and ignotin with salviae miltiorrhizae and ligustrazine hydrochloride injection for acute cerebral infarction.Methods 50 patients with acute cerebral infarction from July 2012 to October 2013 admitted in our hospital were randomly divided into observation group and control group.Observation group had 25 patients,they were treated with cattle encephalon glycoside and ignotin with salviae miltiorrhizae and ligustrazine hydrochloride injection.Control group had 25 patients,they were treated with only salviae miltiorrhizae and ligustrazine hydrochloride injection.After two groups'treatment,the clinical efficacy,indicators of hemorheology (include whole blood viscosity,platelet aggregation and plasma viscosity),neurological deficiency score and activity of daily life were observed and compared.Results There was no significant difference in the clinical data between two group.After treatment,the total efficiency rate in observation group and control group were 92.00% and 60.00%,separately,the difference between two group was significant (u=2.4129,P=0.0158);the whole blood viscosity (high cut, low cut)of observation group were shorter than control group(P<0.05 );platelet aggregation of observation group were shorter than control group(t=0.5632,P=0.5759);plasma viscosity of observation group were shorter than control group(t=3.7682,P=0.0004);the neurological deficiency score and activity of daily life of observation group were shorter than control group(t =3.7682,P=0.0004).Conclusion The clinical efficacy of cattle encephalon glycoside and ignotin with salviae miltiorrhizae and ligustrazine hydrochloride injection for acute cerebral infarction is well.

Objective To investigate the protective effects of aerobic exercise and miR-222 expression in myocardium of diabetic mice. Methods C57BL/ 6 mice were divided into 4 groups: normal non-exercise group (SC), normal exercise group(EC), non-exercise diabetic group(SD), and exercise diabetic group(ED). After the diabetic model was established successfully, EC and ED underwent a swimming training for 5 weeks. By the end of the experiment, light microscope was used to observe the pathological changes of heart, RT-PCR for myocardial miR-222 expression, and Western blot for phosphatase and tensin homolog deleted on chromosome ten ( PTEN ), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) protein expressions in myocardial tissue. Results (1) Under the light microscope, the diabetic mice had a significant change in myocardial structure, with great disorder in the cell arrangement. After exercise intervention, the lesion was alleviated. (2) MiR-222 expression was increased in the myocardium of normal mice and DM mice after exercise (all P<0. 05); (3) Compared with SC group, PTEN expression was increased and PI3K/ Akt expressions were inhibited in myocardium of diabetic mice(all P <0. 05). After exercise intervention, the expression of PTEN reduced( P < 0. 05) and PI3K/ Akt pathway was reactivated in myocardium of diabetic mice (all P<0. 05). Conclusion Exercise intervention may protect the myocardium under high glucose via inducing miR-222 and activating PI3K/ Akt signaling pathway.

MicroRNAs (miRNAs) are a class of endogenous;non-coding small RNA molecules;which can degradate target mRNA;or negatively regulate the expression of the corresponding target genes in the post transcriptional level through inhibiting target gene translation;inducing degradation and so on.MiRNAs exert important parts in the process of metabolism;cell growth;and development.MicroRNA 222 (miR-222) is an important member of microRNAs;which plays an important role in cell proliferation;differentiation;apoptosis and the development of a variety of biological tissues.Recent studies found that miR-222 could mediate a variety of physiological and pathological processes;and significantly influence the development of cardiovascular disease.MiR-222 has an important role in inflammatory response and apoptosis.Here;we reviewed the relationship between miR-222 and cardiovascular disease.

Objective To study and compare the present situation and the difference in subjective well-being between students in junior high schools for the blind and normal junior high school students, and provide basis for the construction of education mode for the well-being of the blind students. Methods By random sampling method, subjective well-being questionnaire was used to investigate the 155 blind students in five blind schools and 436 normal junior high school students in three junior high schools in Shandong Province. Results ( 1 ) The blind students had shown significant differences in many aspects of subjective well-being such as gender, family e-conomic status, sight and parents'education degree (P<0. 05) . (2) Blind school students'social confidence experience (4. 59 ±1.13) was significantly higher than that of the normal students (4.31 ±1.13), and had a significant difference (P<0.05). Blind students got significantly lower scores ((4. 38 ± 1.26) , (4.00 ± 1. 29) ,(3. 58 ± 1. 37) ,(3.89 ± 1. 35) ,(4.41 ± 1.04) , (4. 20 ± 1. 33) ) than normal school students( (4.68 ± 1. 19) , (4. 36 ±1.14),(3.88±1.27),(4.41 ±1.25),(4.61 ±0.99), (4. 52 ± 1. 18)) in target value experience, physical health experience, mental health experience, interpersonal adaptability experience self-acceptance experience, and emotional balance experience. Conclusion There exists a significant difference between the blind students and the normal junior high school students in the different aspects of subjective well-being.

ObjectiveTo provide evidence for the education on sychological health for the undergraduates by analyzing the relationship among interpersonal relationship, coping style, self consistency and congruence of local college students. Methods uestionnaires about interpersonal relationship, coping style, and self consis tency and congruence have been carried out on a stratified random sample of 1471 students from four local colle ges. And all the data of the questionnaire scales will be dealt with by the software SPSS15.0. Results①Total scores on interpersonal relationship and self consistency and congruence of male college students( (9.88 ±5.51 );(93.29 ± 13.48 ) respectively) were much higher than the scores of the female college students ( ( 8.84 ± 5.11 );(90.09 ± 12.60) respectively). Total scores of the above two items for the seniors( ( 10.26 ± 5.89 ); ( 96.79 ±12.93) respectively) were obviously higher than the total scores of students of other grades. For the engineering students, whose total scores on the two items( ( 10.26 ±5.86); (93.57 ± 12.83 ) respectively) were remarkably higher than the scores of students of other majors. Regarding the various factors of coping style, there are signifi cant differences in gender, grade and major( P ＜ 0. 01 ). ②Concerning the college students, there were conspicu ous relationship among their interpersonal relationship, coping style, self consistency and congruence ( P ＜ 0.01 ). ③ Self consistency and congruence could be effectively predicted by factors, i.e. total score of interpersonai rela tionship, remorse, problem-solving, rationalizing, fantasy, recoil, help-seeking and grade. ConclusionCollege students' ability of self consistency and congruence is affected by their coping style and interpersonal relationship. To improve the individual' s ability of self consistency and congruence and the condition of their psychological health, the most effective way is to cultivate the capability on interpersonal communication and apply the positively flexible coping style.

Periventricular leukomalacia ( PVL) is the most distinctive and characteristic brain injury in premature infants, which may result in nervous system sequelae, and affect motor development and quality of life of the infants. PVL lacks specific symptoms and the diagnosis of PVL depends on imagine. Transcranial ultrasound could make initial diagnosis and prognostic evaluation for PVL. Combined with MRI, it can assess the degree of the white matter damage and predict the possible adverse consequences, which could provide evi-dence for early treatment. The changes of brain imaging and their impact on the prognosis of PVL in preterm newborn was reviewed in this article.

Objective To understand the cooperation experiences of doctors and nurses from the Infectious Department,that may be contributive to promote a harmonious relationship between doctors and nurses.Methods Sixteen nurses and nine doctors came from Infectious Department were interviewed.The data were collected through face-to-face in-depth semi-structured interviews.Thematic analysis based on Qualitative Research method,qualitative content analysis was used for data interpretation by analysis and management.Results Three themes were identified and described as the cooperation experiences between doctors and nurse,the cooperation troubles,suggestions about promoting a harmonious relationship between doctors and nurses.Conclusion Harmonious relationship is particularly important in the clinical work,but in fact,there are many factors affect the harmonious relationships,so it is recom-mended that hospital leaders should take measures to build a harmonious relationship between doctors and nurses.

OBJECTIVE:To provide reference for reasonable iron supplement during pregnancy. METHODS:5023 iron sup-plement prescriptions for 800 delivery women receiving antenatal care and childbearing in our hospital in 2014 were analyzed retro-spectively. According to international authoritative guideline for gestational iron supplement,the rationality of iron supplement for pregnant women in our hospital was investigated in terms of timing,duration and dosage. RESULTS:The iron supplement timing and duration of 800 pregnant women all met the criteria;among 5023 iron supplement prescriptions,62 prescriptions of preventive iron supplement and 3875 prescriptions of iron supplement for iron deficiency were up to the standard. CONCLUSIONS:The dos-age of gestational iron supplement are unreasonable in our hospital at a certain extent. It is necessary to strengthen clinical medica-tion management to guarantee safe and rational iron supplement in pregnant women.

AIM: To study the protective effect of aerobic exercise on cardiac dysfunction in mice and its mechanism, and to provide theoretical and practical basis for the exercise therapy of diabetic cardiac dysfunction .METH-ODS:The mice were divided into normal control non-exercise (NNC) group, normal control exercise (ENC) group, dia-betic non-exercise (NDM) group and diabetic exercise (EDM) group.At the end of the experiment , the cardiac function was evaluated by echocardiography .The pathological changes of the myocardial tissues and the development of fibrosis were observed.The mRNA expression of ANP, and the protein levels of PI3K (p110α) and Akt were determined.RESULTS:The decrease in cardiac function of diabetic mice was observed , and the cardiac function recovered after exercise interven-tion (P<0.05).Under light microscope with HE and Masson staining , the myocardial structure in NDM group was in ex-treme disorder , cell arrangement was not neat , and the degree of fibrosis increased , but the myocardial damage was im-proved in ENC group .Compared with NNC group , the mRNA expression of ANP in the myocardium of diabetic mice was up-regulated (P<0.05).The protein levels of PI3K (p110α) and Akt were decreased (P<0.05), and the cascade was inactivated.Compared with NDM group , the mRNA expression of ANP was down-regulated and the protein levels of PI 3K (p110α) and Akt were up-regulated in EDM group (P<0.05).CONCLUSION:Diabetes results in myocardial damage in mice, and reduces cardiac function .Exercise intervention alleviates the heart dysfunction induced by high glucose via activating PI3K( p110α)/Akt signaling pathway to protect the structure and function of the myocardium .