Objective To investigate the role of FHIT,cyclinD1,CDK4 in NSCLC.Methods Immunochemical(S-P) was used to determined 81 cases of NSCLC.Results The postive expression rates of FHIT,cyclinD1,CDK4 were significantly different between in NSCLC and in nomal lung tisses(P

Professional personality is characterized by stability and plasticity,and it should make the entry point of the cultivation of medical students' professional personality on the training of doctor-patient communication skills.Meanwhile,doctor-patient communication skill is an important factor of the medical professional personality.This paper discussed the cultivation of doctor-patient communication skills among medical students from the perspective of analysis of doctor-patient communication obstacles.It should improve the doctor-patient communication skills of medical students and shape healthy medical professional personality,taking the education of cognition,emotion,will,and behavior as pathways.

0.05), but there is statistic difference between the two groups in estimating the involvement of cervical canal and the earlier surgical stage than clinical stage(P

OBJECTIVE:To detect and analyze the drug resistance of the AmpC and the extended-spectrum ?-lactamases(ESBLs)in Escherichia coli(E coli)from urinary infections.METHODS:ESBLs was detected by Phenotypic Confirmatory Test and AmpC was detected by improved three dimensional test.RESULTS:The isolating rates of ESBLs(45 strains),AmpC(8 strains)and AmpC+ESBLs in E.coli(5 strains)were 34.6%,6.2% and 3.8%,respectively.The drug resistance rates of ESBLs and AmpC-producing E.coli was higher than those of the strains without producing ESBLs or AmpC.CONCLUSION:Production of AmpC and ESBLs in ?-lactam antibiotics was the main resistance mechanism against E.coli.Carbopenems should be regarded as the first choice in clinical empirical medication for enzyme-producing strains.

Objective To investigate the expressions and significance of PAX-5, OCT-2 and BOB.1 in Hodgkin lymphoma (HL). Methods Immunohistochemical method was used to detect the expressions of CD30, CD15, PAX-5, OCT-2, BOB.1, CD20 and CD3 in 44 samples of formalin-fixed, paraffin-embedded tissues of HL. The histological classification was carried out according to the WHO classification for tumors of hematopoietic and lymphoid tissues (2001). Results In all 44 cases, 3 (6.8) were nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) and 41 (93.2) were classical Hodgkin lymphoma (CHL). In Hodgkin and Reed Sternberg cells, CD30 were expressed in 36 samples (81.8), CD15 in 27 (61.4), CD20 in 14 (31.8), PAX-5 in 43 (97.7), OCT-2 in 14 (31.8), and BOB.1 in 7 (15.9). All samples of NLPHL expressed OCT-2 and BOB.1, while in the samples of CHL, 30 samples (73.2) expressed both OCT-2 and BOB.1, 7 (17.1) expressed only OCT-2, 1 (2.4) expressed only BOB.1 and all were negative for CD30. Conclusion Hodgkin lymphoma is a rare neoplasm with uncertain cause and difficult to diagnose. The detection of PAX-5, OCT-2 , BOB.1 in combination with CD30, CD15 will conduce to the diagnosis and differential diagnosis of Hodgkin lymphoma.

Objective To explore the association of T cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) gene polymorphism and IFN-γ levels with the incidence of primary nephrotic syndrome (PNS) in children. Methods A case-control study was conducted and 21 Han patients with PNS were selected and included in case group. Meanwhile, 20 each from Mongolian and Han were selected and assigned into control group and at least three generations of their family members were from the same nationality. PCR-restriction fragment polymorphism analysis was used to detect and analyze single nucleotide polymorphisms of exon -574A/C in TIM-3 gene in PNS children and controls. Also the genotype and allele frequencies between the two groups were compared. Enzyme linked immunosorbent assay (ELISA) was used to detect the level of serum IFN-γ and its changes was analyzed. Results There was no significant difference in the distribution of genotypes (AA, AC, CC) of exon -574A/C in TIM-3 gene between the Han and Mongolian subgroups in control group (P=0.741). Neither did the allele frequency between the two groups (P=0.655). Compared with control group (Han and Mongolian), the frequencies of AA, AC and CC genotypes were 9.52%, 28.57% and 61.90% respectively in -574A/C loci of the Han nationality children with PNS. There was significant difference in genotypes distribution between the two groups (P=0.017). The frequency of C allele in PNS children of Han nationality was 76.2% which was higher than that in normal control group (50%), and the difference was statistically significant (P=0.005). Compared with A allele carriers, the risk of PNS in C allele carriers increased by 3.20 times (95%CI: 1.39～7.37). There were no significant differences in serum IFN-γ among the Han nationality with PNS, Han and Mongolian normal control groups (P>0.05). Conclusion The single nucleotide polymorphism of the exon -574A/C of TIM-3 gene may be related to the pathogenesis of PNS in children. In addition, IFN-γ is not associated with the incidence of primary nephrotic syndrome in children.

Objective To investigate the role of FHIT in NSCLC and the relativity between FHIT and cigarette.Methods 81 cases of NSCLC and 7 cases of the nomal lung tisses were examined for FHIT by immunochemical(S-P).Results The postive expression rates of FHIT were significantly lower than those in nomal lung tisses(P

Objective To investigate the pregnancy opportunity and outcome of patients with sysmetic lupus erythematosus (SLE).Methods We carried out a retrospective analysis of the clinical information of patients who suffered from SLE complicated with pregnancy and were hospitalized to People's Hospital of Peking University from December of 1992 to February of 2012.Chi-square test were used for statistical analysis.Results Forty-nine cases of patients with SLE complicated with pregnancy had 52 pregnancies in total.In 27 cases of planned pregnancies,24 cases(89％ )resulted in live births and 5 cases( 18％ ) had flares during pregnancy.Of the 24 live births,3 cases( 12％)were premature deliveries and 4 cases(17％) had low birth weight infants.In 25 cases of unplanned pregnancies,12 cases (48％) resulted in live births and 20 cases (80％) had flares during pregnancy.Of the 12 live births,6 cases(50％ ) were premature deliveries and 6 cases (50％) were low birth weight infants.The patients in the planned pregnancy group tended to have flares during pregnancy more frequently than those in the unplanned group (P＜0.01),the former were more likely to have live births than the latter (P＜0.01) and were less likely to have premature delivery (P＜0.05).Conclusion Patients with SLE should have planned pregnancy and need collaborative supervision of both rheumatology and immunology department and obstetric department to improve live birth rate,decrease premature delivery and the activity of SLE during pregnancy.

Objective To analyze the advantage of altered fractionation radiotherapy by calculating the accumulative effects of daily biologically effective dose (BED) to find out the difference between conventional fractionated radiotherapy and altered fractionation radiotherapy.Methods The data in the report of hyperfractionated or accelerated radiotherapy for head and neck cancer published by Cochrane Collaboration in 2010 was analyzed.Based on the radiotherapy processes mentioned in this report,the accumulative effects of daily BED were calculated and compared in different radiotherapy processes by using linear-quadratic mode.The variation of BED in different radiotherapy processes was find out.Results In total dose of unity as the premise of 70 Gy,altered fractionation especially the hyperfractionated accelerated radiotherapy could give a higher BED to the tumor during a shorter period,hyperfractionated radiotherapy could give a lower BED to normal tissues,and hyperfractionated radiotherapy with split course could give higher BED to the tumor while lower BED to normal tissues.Conclusions The variation of BED in different radiotherapy processes can be shown clearly by linear-quadratic mode.It can be simple and shortcut through mathematical models for the evaluation of different radiotherapy plan,on clinical symptomatic selection play a guiding role in tumor therapy.

Objective To study the difference in the measurement of the absorbed dose in water between the dosimetry protocols of IAEA TRS-398 and TRS-277 for high-energy electron beams.Methods The differences were compare in absorbed doses from 6 kinds of electron beams among the three methods of using cylindrical chamber,plane-parallel chamber and cross-calibrated plane-parallel chamber which was calibrated in user' s high-energy electron beam according to IAEA TRS-398 and IAEA TRS-277.Results The difference in absorbed doses measured,following the two protocols,was 0.4％-2.3％ for planeparallel chamber,0.6％-2.2％ for cylindrical chamber,and 0.5％-2.0％ for cross-calibrated chamber.The differences in measured absorbed doses between the two dosimetry protocols were slight.Conclusions The methods used to determine absorbed dose to water recommanded by IAEA TRS-398 for high-energy beams are more accurate and more suitable for clinical users to measure compared to the TRS-277.