The anti proliferation effects of four different concentrations of tea polyphenols on PG cell lines were determined with MTT assay. Laser scanning confocal mircroscopy and flow cytometry techniques were used to determine the changes in intracellular calcium concentration, GJIC, Cx43 expression, and cell cycle distribution after treatment with tea polyphenols. The results showed that four different concentrations dose dependently inhibited the proliferation of PG cell lines. It caused a decline in the proportion of cells in S and G2/M phase, blocked the cell cycle progression in G0/G1 phase, and reduced the proliferation index of PG cell lines. Compared with control group, intracellular calcium concentration, GJIC and Cx43 expression were gradually increased ascended with an increase in tea polyphenols concentration. The results suggested that tea polyphenol could inhibit growth of PG cell lines. The mechanism of anti tumor is associated with an up regulation of GJIC of PG cell line.