ObjectiveTo observe the clinical application of amiodarone combined with benazepril to paroxysmal atrial fibrillation. Methods80 patients were randomly divided into observation group and control group of 40 caess ,the control group only used amiodarone treatment and observation group used amiodarone combined with benazepril,compared 24h, 1、3、6、12months after treatment, sinus rhythm conditions and adverse reactions. ResultsThe observation group in sinus rhythm cardioversion rate was 20.0％ (8/40), control group was 15.0％ (6/40) ,it had no significant difference between the two groups at 24h( x2 =0. 346 ,P =0. 556) ;observation group in sinus rhythm cardioversion rate was 57.5％ ( 23/40 ) ,67.5％ ( 27/40 ) ,62.5％ ( 25/40 ) ,57.5％ ( 23/40 ), the control group was 32. 5％ (13/40) ,40.0％ (16/40) ,27.5％ (11/40) ,30.0％ ( 12/40 ) at 1,3,6,12 months, it had statistically difference betweem the two groups ( x2 =5.051,6.084,9.899,6.146, P =0. 025 ,0. 014 ,0. 002 ,0. 013 ). 1 case of observation group with gastrointestinal discomfort, 3 cases of control group with nausea and vomiting occurred in,4 cases with bradycardia,2 cases with QT interval prolongation,the incidence of adverse reactions in observed group was lower than the control group( x2 =5. 600,P =0.018). ConclusionThe clinical effect of amiodarone combined with benazepril treatment of paroxysmal atrial fibrillation was better than amiodarone alone,it could effectively reduce the adverse reaction, so it was worthy of attention.

Astragalus mongholicus with red or green stalks and A.mcmbramaceus with or witho ut down on it leaves were differentiated by comparing their pollen, vein on their leaf surf ace, scanning their stigma and ovary with election microscope and analyzing their isoenzy mes aud lipases

Mitochondria, the power house of cells, are important organelles in eukaryotic cells. Having their own unique and complete DNA (mtDNA) and genetic system, mitochondria play an essential role in cellular energy metabolism, intracel?lular signaling and apoptotic pathways, as well as many other biological functions, which are closely related with cellular met?abolic network. A disruption of mitochondrial genes can therefore result in mitochondrial dysfunction and human diseases, thus they have been widely used in molecular biology, development biology, genetics, forensic identification and clinical diag?nosis. Consequently, sequencing mitochondrial genome has shown great significance in mitochondrial structure and function research. In this review, research progress in mitochondrial genome sequencing method is summarized, mainly focusing on Sanger sequencing, long-PCR and next-generation sequencing. Also rolling circle amplification and indirect sequencing of mtDNA are reviewed. The ambiguities caused by numts in indirect sequencing are mentioned and resolved.

Objective To investigate the expression profile of mRNAs in brain samples collected from pronuclear transfer (PNT) mice. Methods Female CD-1 mice were superovulated, and zygotes were collected after mating with adult male mice. Zygotes with two pronuclei were selected for pronuclear transfer manipulation, and then the reconstructed zygotes were transferred into the oviduct of pseudopregnant female mice. The infant mice obtained from pronuclear transfer were called PNT group, while the embryoes that were not performed pronuclear transfer was regarded as control group. Total RNA were extracted from brain samples of both PNT and control mice, and cDNA were labeled with fluorescent dye. Genes that were differentially expressed were identified using the Agilent mouse mRNA array. Gene ontology analysis and pathway analysis were also completed. Results Compared with control group, 392 mRNAs were expressed differentially, which showed more than 2.0 times variation and statistical significance, accounting for 1.7% of all mRNAs. Among those 366 mRNAs were up-regulated and 26 mRNAs were down-regulated. Eleven mRNAs came to 4.0 times variation in total. Gene ontology analysis indicated that differentially expressed genes were significantly enriched in alternative mRNA splicing, small GTPase mediated signal transduction, regulation of insulin receptor signaling pathway, hydrolase activity, transmembrane transporter activity and pyrophosphatase activity. Significant enriched pathway terms contained ion channel transport, fatty acid metabolism, butanoate metabolism, triacylglycerol and ketone body metabolism. Conclusion Pronuclear transfer might influence some key metabolism process in mouse brain.

The fasting and 2 h levels of glucagon, somatostatin ( SS), and C-peptide during 75 g oral glucose tolerance test in 60 patients with type 2 diabetes and 34 normal subjects were determined. Compared with control group, the fasting levels of glucagon and SS and 2 h levels of SS after glucose loading significantly decreased,while the fasting and 2 h levels of C-peptide increased in diabetes group. The 2 h levels of these hormones were significantly higher than the fasting levels in two groups. Compared with control group, the increased folds of glucagon ( 1.40±0.48 vs 1.20±0. 30, P＜0. 05 ) and SS( 2.79±2. 17 vs 1.14±0. 22, P＜0. 01 ) levels after glucose loading were higher and that of C-peptide level ( 3.58 ±3. 10 vs 8. 33 ± 6. 99, P＜0. 01 ) was lower in diabetes group. The levels of fasting glucagon were positively correlated with that of fasting SS in two groups( both P＜0. 01 ). These results suggest that disturbance exists in hormones from α and δ cells besides the dysfunction of β cells in patients with type 2 diabetes.

OBJECTIVE To understand and use reasonably the strategy of prevention and management of overwhelming postsplenectomy infection(OPSI). METHODS According to intervention to patients with postsplenectomy by means of education,vaccination,antibotic prophylaxis after April 1998,clinical and follow-up data were reviewed and analyzed from 337 cases patients with traumatic splenectomy from Jan 1992 to Jan 2004,and correlative factors of four OPSI cases were further analyzed. RESULTS Incidence of OPSI descended obviously after intervention(P