Objective To explore the relevance between resting heart rate (RHR) and metabolic syndrome in young and middle-aged patients with essential hypertension.Methods Three hundred and forty patients with essential hypertension were enrolled in this study.According to the RHR,the hypertension patients were categorized into 3 groups:group RHR 1 (RHR ＜ 70 bpm,52 cases),group RHR 2(RHR 70-85 bpm,168 cases) and group RHR 3 (RHR ＞ 85 bpm,120 cases).The RHR,blood pressure,fasting blood glucose,triglyceride,high density lipoprotein cholesterol,2 h postprandial blood glucose and waistline were determined and compared.Results The level of fasting blood glucose,2 h postprandial blood glucose,triglyceride,waistline,systolic pressure,diastolic pressure,and high density lipoprotein cholesterol in group RHR 1 were (4.96 ± 0.65) mmol/L,(6.85 ± 0.51) mmol/L,(1.69 ± 0.43) mmol/L,(78.3 ± 6.5) cm,(143.2 ± 5.6) mmHg (1 mmHg=0.133 kPa),(85.4 ±5.2) mmHg and (1.08 ±0.64) mmol/L.In group RHR 2 they were (5.66 ± 0.77) mmol/L,(7.94 ± 0.66) mmol/L,(2.58 ± 0.75) mmol/L,(88.6 ± 7.3) cm,(156.7 ± 6.1) mmHg,(93.6 ± 4.7) mmHg,and (0.95 ± 0.59) mmol/L.In group RHR 3 they were (6.85 ± 0.73) mmol/L,(8.97 ± 0.59) mmo]/L,(3.52 ± 0.90) mmol/L,(95.8 ± 9.2) cm,(164.3 ± 7.4) mmHg,(101.2 ± 3.5) mmHg and (0.78 ± 0.33) mmol/L.With the increase of RHR,the level of fasting blood glucose,2 h postprandial blood glucose,triglyceride,waistline,systolic pressure and diastolic pressure increased step by step,high density lipoprotein cholesterol decreased step by step,and there were significant differences (P ＜ 0.05).Correlation analysis showed that RHR was positively correlated with fasting blood glucose,2 h postprandia blood glucose,triglyceride and waistline (r =0.52,0.45,0.50,0.36,P ＜ 0.05),and negatively correlated with high density lipoprotein cholesterol(r =-0.39,P ＜ 0.05).Conclusion RHR is closely related to the metabolic disorder in young and middle-aged patients with essential hypertension,and doctors should pay attention to the heart rate control while lowering blood pressure.

Pulmonary embolism(PE)is a relatively common cardiovascular emergency.Haemodynamic instability is the characteristics of massive pulmonary embolism,with a mortality as high as 20%.The goals of PE treatment are to remove the thromboembolic obstruction rapidly,exert beneficial effects on haemodynamic parameters and save the lives of patients.Haemodynamic and respiratory support is of vital importance in some critically ill patients with PE.The basic treatment is anticoagulation,the methods of which are different for PE during pregnancy,among cancer patients,with right heart thrombi and with heparin-induced thrombocytopenia.

Objective To optimize the medium for rhizomatous induction of Belamcanda chinensis in vitro. Methods By plant tissue culture technology, the effects of various carbon source, NAA, and active carbon at different concentration on the rhizomatous formation of B. chinensis in vitro were studied. Results MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+6% white sugar was the optimal medium for the rhiz-omatous formation of B. chinensis in vitro. Active carbon should not be added to the medium. The germination rate of rhizomatous in vitro was 61.03% on the MS + BA 2.0 mg/L+3% white sugar. Conclusion Sugar concentration is the main factor of the influence on the rhizomatous formation of B. chinensis in vitro.

Objective To study the molecular pathologic features of CD71 and the correlation between CD71 expression and IgA deposit in IgA nephropathy(IgAN),and to investigate the role of CD71 in pathogenesis of IgAN in order to direct the therapeutic strategies.Methods According to clinical manifestations and pathological diagnosis,170 cases of renal biopsy were divided into IgA deposition nephritis(primary group),non-IgAN with IgA deposition(secondary group)and no IgA deposited nephritis(control group).Immunofluorescence double-staining using TRITC-conjugated anti CD71 antibody and FITC-conjugated anti-human IgA antibody was performed to illustrate the expression of CD71 and IgA respectively by confocal microscopy.Results No CD71 expression was found in control group,while the CD71 was positively expressed in primary and secondary groups to various levels.The intensity of expression of CD71 in primary group was more intense than that in the secondary group.The expression of CD71 in the IgAN tissues correlated closely with the accumulation of IgA.CD71 and IgA were distributed in mesangial area and capillary lumina of glomeruli of IgAN(primary group).The study showed that CD71 expression and IgA deposition were co-localized under confocal fluorescence microscopy.Conclusion CD71 is highly expressed in the glomeruli of primary IgAN.The expression of CD71 in the IgAN tissues correlats closely with the accumulation of IgA.CD71 and IgA are co-localized under confocal fluorescence microscopy.The present study reveals that CD71 is one of IgA receptors and involves in the course of IgAN.The results suggests that CD71 may play an important role in immunopathogenesis of IgAN.

Objective To study some biological properties of mouse bone marrow stromal cells(MSCs)and their ability to differentiate endothelial cells and surviving ability in ischemic tissue,and provide an experimental foundation for applying MSCs to ischemic repair.Methods After the tibias and femurs were dissected from 5-to 6-week-old mice from Jan.2005 to Nov.2005,the marrow was flushed out with ice-cold DMEM/F12 medium.The mononuclear cells of the marrow were obtained with density gradient centrifugation and the plated and cultured in DMEM/F12 medium.The cultured cells in vitro were induced with endothelial cell growth supplement.The ability of MSCs was also was examined in ischemic tissue.Results The adherent fibroblast-shaped cells approached confluence in single layer 12~16 d after plating.The cultured MSCs in vitro differentiated into endothelium.Numerous scattered DAPI-labeled cells were found in the specimen seven days after implantation,and hematoxylin and eosin staining of the adjacent section showed concordance between dense hematoxylin staining and presence of DAPI epifluorescence,and there was no obvious inflammatory response.Conclusion The subcultured MSCs possess potential to differentiate into endothelial cells.MSCs show stable growth in vitro,easy survival in the subculture and rapid proliferation in present culture condition.MSCs may be used in therapy for myocardium ischemia.

OBJECTIVE: To develop a rapid, accurate and sensitive method for the determination of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally. METHODS: The LC-MS method was used to detect the extractive of Chinese patent medicine for antihypertension in respects of relative molecular mass, tandem mass spectrometry fragment, retention time, UV spectrum. The compounds added into the Chinese patent medicine were identified by comparing with standard sample in terms of spectrum, chromatographic and mass spectrometric behavior. RESULTS: According to four aspects of determination, nifedipine, nitrendipine and nimodipine were found in three kinds of Chinese patent medicine for antihypertension. CONCLUSION: The method is selective and sensitive for the detection of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally.

The aim of this study was to investigate the role of adhesion molecules P selectin (CD62P) in the pathogenesis of primary nephrotic syndrome (NS) and its relationship with its different pathological types. The expression of CD62P in renal biopsy tissue and peripheral blood platelets from patients with NS were measured with direct immunofluorescence labelling monoclonal antibody and flow cytometry respectively. The results showed that CD62P was expressed in 140/165 cases with NS (84 8%). Among these patients, renal expression of CD62P was lower in MN and FSGS patients than that in MPGN and MsPGN patients, and even lower in MCD patients. The expression of CD62P in peripheral blood platelets of patients with NS was significantly higher than that in the normal control group( P

OBJECTIVE To discuss the antibacterial and anti-inflammatory effects of GanKeQing granules.METHODS The antibacterial tests in vitro,and the tests of it′s affects for auricular swelling induced by xylene and celiac capillary permeability induced by acetic acid in mice were done to observe the pharmaceutical effects of GanKeQing granules.RESULTS GanKeQing granules could inhibit and kill 5 kinds of pathogenic bacteria,suppress the swelling of the mouse′s ear caused by xylene and releave the enhancment of mice capillary permeability caused by acetic acid.CONCLUSIONS GanKeQing granules have better antibacterial and anti-inflammatory effects,which could be used for the treatment and prevention of cold and flu.

The neonatal burst suppression is a severe EEG pattern and always demonstrates serious damage of nerve system. But the outcome of these patients depends on the different etiology. A total of 256 cases of video EEG recordings were analyzed in order to summarize the etiology and outcome of burst suppression. The results showed that some patients in all 17 cases of burst suppression showed EEG improvement. The etiology was the dominant factor in long term outcome. It was suggested that effective video EEG monitoring is helpful for etiologic study and prognosis evaluation.
Electroencephalography/*methods ; Hypoxia-Ischemia, Brain/diagnosis ; Monitoring, Physiologic/methods ; Neonatology/methods ; Video Recording

Objective To approach the related factors and emergency endoscopic hemostasis measures for duode-nal papilla hemorrhage after ERCP, then sum up the clinical experience of prevention and treatment. Methods Clin-ical data of 27 patients with duodenal papilla hemorrhage treated by emergency endoscopic hemostasis were retro-spectively analyzed. Results 26 cases treated by emergency endoscopic hemostasis were successful, the success rate was 96.30 %. Conclusions Prevention is very important for duodenal papilla hemorrhage. Once bleeding after ER-CP, emergency endoscopic hemostasis is the most direct, simple and effective measure.