Objective To study the effects of monosodium glutamate on viability and fertility of filial generation Drosophila.Methods Different concentrations(15,30,45 g/L)of L,H,T monosodium glutamate(MSG)were added in the medium for wildtype Drosophila melanogaster,and the non-MSG medium was taken as the control group,3 pairs of Drosophilas in each bottle and 3 repeats in each group,the first and second filial generation adult Drosophila's eclosion time(growth period) and the number of offspring within 7 days(reproduction quantity),and the death count in 30 days of 20 Drosophilas were observed and recorded.Results Compared with the control group,the reproduction quantity of Drosophila significantly increased,the growth period shortened in the first filial generation in 15 g/L of T MSG group.As the MSG concentrations increased,the reproduction quantity reduced significantly,the survival rate of Drosophila in 30 days declined significantly.The reproduction quantity in second filial generation increased at the initial stage of exposure and then decreased as the MSG concentrations increased.Conclusion The results of the present paper indicates that low-dose of MSG may be the advantage for survival and reproduction of Drosophila,but high doses will produce adverse effects.

Objective: To evaluate the effects of lotensin on sympathetic responses following cervical plexus block and to explore the mechanism of cardiovascular responses and sympathetic nervous system activity according to plasma catecholamine concentration. Method: Sixty adult patients were randomly divided into two groups (n = 30):Group A and group B. In group B lotensin 0.15mg/kg was taken orally at night before surgery and 2h before anesthesia separately. Hemodynamics was determined before anesthesia(T_1), 5min(T_2), 10min(T_3), 15-20min(T_4)and 30min (T_5) after cervical plexus block. For measurement of plasma catecholamine concentration, blood was collected at T_1,T_3,T_4 and T_5. The study was finished before beginning of surgery and infusion. Result: In group A after anesthesia SP,DP, MAP,HR,RPP and plasma noradrenalinc level rose markedly(P0.05). All parameters were lower in group B compared with group A(P

Correlation between TCM typing of chronic gastritis and the findings about the mucosa in gastroscopic examination was analyzed. In GLM and DUNCAN analyses, the sufferers of insufficiency of both the spleen and stomach were much older than of the damp - heat in the spleen and the stomach (P

Objective To explore the dynamical progeression and clinical significance of S100B and IL-1? of cerebrospinal fluid in patients with tuberculous meningitis.Methods 43 patients with TM(TM group)treated with tubercle drug and 28 patients without never diseases(control group)have been studied.S100B in Cerebrospinal Fluid were exeminated by ELISA and IL-1?by radio-immunassay.Results S100B or IL-1? of cerebrospinal fluid in patients with tuberculous meningitis increased evidently in contrast with the control group.And S100B or IL-1? of Cerebrospinal Fluid in coma patients increased evidently in contrast with no coma.Conclusion The levels of S100B in CSF could reflex the degree of damage in patients with TM.IL-1? was more sensitive to treatment.

OBJECTIVE To discuss the antibacterial and anti-inflammatory effects of GanKeQing granules.METHODS The antibacterial tests in vitro,and the tests of it′s affects for auricular swelling induced by xylene and celiac capillary permeability induced by acetic acid in mice were done to observe the pharmaceutical effects of GanKeQing granules.RESULTS GanKeQing granules could inhibit and kill 5 kinds of pathogenic bacteria,suppress the swelling of the mouse′s ear caused by xylene and releave the enhancment of mice capillary permeability caused by acetic acid.CONCLUSIONS GanKeQing granules have better antibacterial and anti-inflammatory effects,which could be used for the treatment and prevention of cold and flu.

Objective:To explore the influence of different cell culture methods in the genome-wide DNA methylation status of breast cancer MDA-MB-231 cells,and to clarify the relationship between genome-wide DNA methylation status and cell growth environment and the role of genome-wide DNA methylation status in the occurrence and development of tumor.Methods:The MDA-MB-231 cells were cultured with 2D and 3D cell culture models and mouse orthotopic transplantation model (Ti model)and collected, then DNA was extracted by DNA extraction kit and the genome-wide DNA methylation status of MDA-MB-231 cells after cultured with three different culture methods was detected by DNA methylation chip,then the value of beta,DiffScore and Delta_Beta of the CpG loci of each gene were calculated by applying Genomestudio software, and the differential methylation genes were screened by Genomestudio software and GO and Pathway analysis of these genes were performed in DAVID on-line analysis tool.Results:All 480 genes of the MDA-MB-231 cells showed significant differences in the degree of methylation in 3D and 2D models (P<0.05);86 448 genes in 3D and Ti models (P<0.05);90 005 genes in Ti and 2D models (P<0.05).The differential methylation genes in 3D and 2D,3D and Ti,and Ti and 2D models were enriched on the multicellular organismal development term and cell differentiation term (P<0.05);also on MAPK signaling pathway,cell adhesion molecules (CAMs),and regulation of actin cytoskeleton (P<0.05). Conclusion:There are differences in genome-wide DNA methylation status of MDA-MB-231 cells cultured in 2D, 3D cell culture and Ti models.

Objective To analyze the changes of electroencephalographic activities during fully automated simultaneous peripheral arteriovenous exchange transfusion(ET) in neonates with hyperbilirubinemia.Methods A total of 45 neonates who suffered from severe hyperbilirubinemia and underwent fully automated simultaneous peripheral arteriovenous exchange transfusion were studied from March 2009 to March 2016 in Neonatal Intensive Care Unit of Guangzhou Women and Children's Medical Center,and 46 ETs were performed in 45 babies who were divided into 2 groups according to the severity of hyperbilirubinemia:the encephalopathy group and the none-encephalopathy group.Nineteen patients were in the encephalopathy group,in which 11 were male and 8 were female.The other 26 patients were in the none-encephalopathy group,in which 15 were male and 11 were female.Changes in amplitude integrated electroencephalogram(aEEG) during ETs were analyzed,including background activities,sleep-wake cycle (SWC)and seizures.Results Forty-five patients with hyperbilirubinemia underwent 46 fully automated simultaneous peripheral arteriovenous ETs.As a result,total bilirubin dropped from (524.90 ± 110.96)μmol/L before ETs to (245.62 ±78.97) μmol/L after ETs,with clearance rate of 53.2％.And indirect bilirubin dropped from(486.16 ±90.39) μmol/L before ETs to(222.19 ± 79.49) μmoL/L after ETs,with clearance rate of 54.3％.On the other hand,there was no significant difference in the changes of electroencephalographic activities during ETs,including background activities (x2 =0.16,P ＞ 0.05),SWC (x2 =0.71,P ＞ 0.05) and seizures (x2 =0.30,P ＞ 0.05).However,there were significant difference in suppressions on background activities between the encephalopathy group and the none-encephalopathy group(Fisher's exact test P =0.042),though there were no significant statistical differences in SWC or seizures between the 2 groups (x2 =0.65,P ＞ 0.05;x2 =2.07,P ＞ 0.05,respectively).Conclusions In neonatal hyperbilirubinemia,fully automated simultaneous peripheral arteriovenous ET is safe and efficient without significant influence on electroencephalographic activities as a whole.However,background activities are more significantly depressed in infants of bilirubin encephalopathy than that of non-encephalopathy during ET.

Objective To explore the impacts of intensive nutritional intervention on maternal and infant outcomes in women with gestational diabetes mellitus(GDM). Methods From January 2014 to ecember 2014, a total of 518 women with GDM were stratified by age, height, body mass index (BMI), and were divided into treatment group (n=258) and control group (n=260) according to the random number generated by the computer software. Women in control group underwent conservative treatment while those in treatment group were given intensive nutritional intervention including keeping records of eating habits, measurement of blood glucose and regular follow-up. The incidence of pregnancy-related complications and newborn outcomes in both groups were compared. Results Women of the two groups were similar in basic clinical data. The range of gestational weight gain (GWG) [(12.2 ± 4.7) vs. (13.9 ± 5.0)kg] and birth weight of infants [(3 406.4±495.4) vs. (3 494.9±484.7)g] in the intervention group was significantly lower than those in the control group (P<0.01). The rate of reaching recommended target of GWG was significantly higher in the intervention group (60.9%) than in the control group (51.9%, χ2=4.2, P<0.05). There was a significant reduction in glucose-related parameters in both groups (P<0.01). In the intervention group, fasting blood glucose and postprandial blood glucose were reduced from (5.21 ± 0.71) mmol/L, (6.68 ± 0.90) mmol/L to (4.71 ± 0.73) mmol/L,(6.21 ± 0.71) mmol/L (P<0.01), respectively in comparison with the control group, the intervention group had lower incidence of cesarean section (44.6% vs. 53.8%), postpartum hemorrhage (2.3%vs. 6.2%), polyhydramnios (7.8%vs. 13.5%), neonatal hypoglycemia (3.1%vs. 6.5%) and macrosomia (8.1%vs. 13.8%, P<0.05). Conclusions Strengthening nutritional intervention in women with GDM could increase the rate of reaching recommended target of GWG, improve the glucose-related parameters and reduce the incidence rate of pregnancy complications.

Objective:To analyze the mutation of 5′ noncoding region of BCL 6 gene in extranodal diffuse large B cell lymphoma (DLBCL) and the relationship between mutation and clinical characteristics.Methods:Extract DNA from paraffin bedded extranodal DLBCL cases,then PCR and sequenced.Meanwhile retrospective analysis was done.Results:The mutation rate was 13 64%(6/44).The clinical characteristics,such as age、sex、B symptom、bulky tumor mass、stage、the level of LDH、BM involvement and CR,had no significant influence on BCL 6 mutation;the survive time of DLBCL with/without BCL 6 mutation had no obvious difference,but there was a tendency the survival time of mutated patients was longer than that of non mutated (the mean survival time was 74 and 65 02 months,respectively);mutation wasn't an independent prognostic factor.Conclusion:The mutation of 5′ noncoding region of BCL 6 gene maybe partly involves in the mechanism of extranodal DLBCL. [

Objective To investigate the metabolic differences of Tacrolimus in different transplant recipients. Methods A retrospective study was conducted, 30 patients of the organ transplant admitted to organ transplantation center of General Hospital of Chinese People's Armed Police Forces from January 2002 to August 2012 were enrolled, and they were divided into combined liver and kidney transplant (SLK) group, single liver transplant group and single renal transplant group, 10 cases in each group. The SLK group and the simple liver transplantation group were given the same drug regimen, methylprednisolone injection was given during operation, and tacrolimus+ mycophenolate mofetil+ prednisone triple immunosuppressive therapy was taken after operation, on the first day after operation, the initial dose of tacrolimus was given 0.06 mg·kg-1·d-1 divided into 2 times taken orally, and on the third day after operation, the concentration of tacrolimus was detected; after operation group for 2 - 4 days, single renal transplantation group was given tacrolimus the initial dose of tacrolimus 0.15 mg·kg-1·d-1 was divided into 2 parts for twice oral administration, after 2 - 3 days of the above treatment, monitoring the concentration of tacrolimus began. One month after transplantation, the metabolic differences of tacrolimus among the three groups were compared.Results One month after operation, the oral tacrolimus doses (μg·kg-1·d-1: 74.78±32.65 vs. 80.62±24.02, 85.58±16.78) and the monitored blood drug concentration (μg/L: 6.64±2.73 vs. 7.50±3.08, 7.46±3.20) in SLK group were lower than either the single liver transplantation group or single renal transplantation group, but the comparisons among the three groups, there were no statistically significant differences(allP > 0.05).Conclusions In SLK group, the protective effect of transplanted liver on transplanted kidney may be related to the length of postoperative time. Liver transplantation performed within post-operative one month has no protective effect on the transplanted kidney.