Research in lymph node imaging has made great progress in recent years. With the increasing in-depth researches and clinical applications of MRI, PET and molecular imaging, the sensitivity, specificity and accuracy of detecting lymph node metastases have also been improved. Incorporating techniques such as various pulse sequences, contrast enhancement and lymph-node contrast agents has made MRI a superior method for detecting lymph node metastasis of breast cancer in clinical settings.

Objective To explore the differential diagnosis value of DWI ADC for breast lumps,improve the diagnosis of breast cancer specificity.Methods 93 patients with 105 breast tumors were performed with routine MR series and DWI,and the ADC values were measured.Results Lesions ADC values:average ADC value 43 benign lesions in 37 cases was(1.37±0.36)×10-3 mm2/s,the different value between max and min was(0.63±0.19)×10 -3s/mm2.Average ADC value of 62 malignant lesions in 58 patients was(0.90±0.53) × 10 -3 mm2/s,the different value between max and min was(0.91±0.27)×10-3 mm2/s.By statistical analysis,both average ADC values and the max and the min ADC difference value were very significantly different(P＜0.01).Draw diagnosis benign and malignant lesions ROC curves,with ADC values 0.11×10 -3 mm2/s for critical value to judge benign and malignant standard,sensitivity was 67％,specificity was 72％.With 1.0×10- 3 mm2/s for critical value,specificity can raise to 84％,and sensitivity came to 60％.With the maximum and minimum ADC difference value 0.75 × 10-3s/mm2 for critical value,sensitivity was 67％,specificity was 68％.If with 0.80 for critical value,sensitivity was 53％ and specificity 82％.Conclusion The average ADC values of DWI were significant for the identification of benign and malignant lesions.

Objective To explore the related factors that affect the incidence of uterine fibroids to provide a theoretical basis for the clinical diagnosis and prevention.Methods Based on the clinical data selected from June 2014 to July 2016 in pathology of our hospital,159 cases of patients were confirmed with uterine fibroids,a comparative study was employed and the single factor test and the logistic regression analysis were both used to analyze the related risk factors that affecting the incidence of uterine fibroids.Results There were all significant level (P ＜ 0.05) between case and control groups in 40 years to 50 years,the number of abortions (≥2) as well as gynecological diseases,which were the independent factors for the incidence of uterine fibroids.In the present,the regression coefficients of former two factors were 0.186 (P ＜ O.05),and 0.584 (P ＜ 0.05),respectively.The corresponding regression coefficients of breast hyperplasia,vaginitis,cervicitis,endometritis and pelvic inflammatory disease,and other gynecological diseases and gynecological diseases were 0.221 (P ＜ 0.05),O.363 (P ＜ 0.05),O.539 (P ＜ 0.05),0.361 (P ＜ 0.05),and 0338 (P ＜ 0.05),respectively.It suggests that these were independent factors affecting the incidence of uterine fibroids.Conclusions The more the increasing age and the number of abortions,kinds of gynecological diseases infected will increase the risk of patients suffering from uterine fibroids.

Objective To evaluate the safety and effect of enteral nutrition support at early period after gastroenteric operation. Methods 62 patients with gastrointestinal cancer were divided into three groups randomly: control group, TPN group, and TEN group. Patients in TPN and TEN groups received 7 days nutritional support in isonitrogenous and isocaloric. The peripheral vein blood was sampled before operation and 7 day after that to observe nutritional status and T lymphocyte subsets, NK cells, and NK cell activity. Resalts There were no significant difference in the three groups in albumin and anthropomentric measurement, visceral proteins decreased in three groups in postoperative period, but the values of control group was more seriously than TPN or TEN groups (P<0.05). Mean nitrogen balance: - 8.6 ± 3.12g/day in control group, - 4.22 ± 2.02g/day in TPN group, - 3.88 ± 2.35g/day in TEN group (P<0.05). CD3 ,CD4 ,CD4/CD8, NK cells and NK cell activity decreased in postoperative period in three groups, but there are no significant ditference in three groups. Conelasions Enteral nutrition support at early period after gastroenteric operation is safety and effective.

Objective To explore the method of establishing the value of TSH in the diagnosis of neonatal congenital hypothyroidism in Shantou area.Methods Time resolved fluorescence was conducted to detect the level of heel blood thyrotropin(TSH)in 90 798 neonatal from January 2013 to December 2015 in Shantou area.Percentile method and ROC curve were used to establish the value of TSH screening.Results The frequency of TSH screening in 90 798 cases of neonatal showed normal distribution.The average content of TSH was(2.38-t-2.12)mU/L,ranging 0.0-324.2 mU/L;P90 for 3.46 mU/L;P95 for 4.63 mU/L and P99 for 8.88 mU/L.CH incidence rate in Shantou was 1/2 974;the P99 percentile method TSH diagnostic cut-off value was 8.88 mU/L,and the ROC curve diagnostic cut-off value was 8.49 mU/L.Besides,the recall rate analyzed by chi square test showed no significant difference between the P99 percentile method and ROC curves (χa =0.965,P =0.326).While,a significant difference were found between P99 percentile method and kit(χ2 =10.861,P=0.001),ROC curve and kit(χ2 =5.450,P =0.020).The sensitivity of P99 percentile method and ROC curve method was 100.0 ％.The Youden index of P99 percentile method was 96.9 ％.Conclusion The P99 percentile method is more suitable for clinical neonatal diseases of TSH screening.

Objective To investigate the trend of cardiac reserve function during the normal labor.Methods Sixty-three cases were chosen randomly from hospitalized maternal women in the First Affiliated Hospital of Chongqing Medical University from 2010 June to December ( six months).The digital technique of heart sound signal processing was used to analysize cardiac reserve function parameters including the heart rate ( HR),the ratio of the amplitude of the first heart sound to the second heart sound and the ratio of diastolic to systolic duration (D/S) of pregnant women.Results ( 1 ) Comparisons of cardiac reserve function between uterine contractions and relaxations during labor:①Latent phase of labor ( cervix dilation ＜ 3 cm ):HR was ( 87.3 ± 14.0 ) beats/min in uterine contractions and ( 82.8 ± 12.5 )beats/min in uterine relaxations,the ratio of D/S was 1.14 ±0.27 in uterine contractions and 1.21 ±0.22 in uterine relaxations,the comparisons of the above two were statistically significant,P ＜0.05 ; But the ratio of S1/S2 was 2.19 ±0.82 in uterine contractions and 2.28 ±0.81 in ueterine relaxations,the comparison was not statistically significant,P ＞ 0.05.② During early active stage of labor ( cervix dilation 3-6 cm):HR was (89.3 ± 15.4) beats/min in uterine contractions and (83.1 ± 13.5) beats/min in uterine relaxations,the ratio of D/S was 1.09 ± 0.30 in uterine contractions and 1.20 ± 0.27 in uterine relaxations,the comparisons of the above two were statistically significant,( P ＜0.05 ) ;But the ratio of S1/S2 was 2.42 ± 1.08 in uterine contractions and 2.29 ±0.83 in ueterine relaxations,the comparison was not statistically significant ( P ＞0.05 ); ③During late active stage of labor (cervix dilation 6-10 cm),HR was (95.4 ± 18.7 ) beats/min in uterine contractions and (86.2 ± 15.6) beats/min in uterine relaxations,the ratio of D/S was 1.01 ±0.25 in uterine contractions and 1.18 ± 0.25 in uterine relaxations,the comparisons of the above two were statistically significant,( P ＜ 0.05 ) ; But the ratio of S1/S2 was 2.61 ± 1.26 in uterine contractions and 2.67 ± 1.19 in ueterine relaxations,the comparison was not statistically significant ( P ＞ 0.05 ).④ The second stage of labor (cervical dilation ≥ 10 cm ):HR was (109.4 ± 19.7 ) beats/min in uterine contractions and (93.5 ± 16.7 ) beats/min in uterine relaxations,the ratio of D/S was 0.89 ± 0.23 in uterine contractions and 1.14 ±0.26 in uterine relaxations,the ratio of S1/S2 was 3.66 ± 1.37 in uterine contractions and (2.81 ± 1.07 ) in uterine relaxations,the comparisons of all were statistically significant (P＜0.05).(2)Comparison of cardiac reserve function in uterine relaxations of each stage of labor:①Maternal heart rate gradually increased from latent stage of labor to the second stage of labor,and decreased postpartum,the comparison was statistically significant ( P ＜ 0.05 ) ;② The ratio of S1/S2 of maternal gradually increased from latent stage of labor to the second stage of labor,and decreased postpartum,the comparison was statistically significant( P ＜0.05 ) ;③ The ratio of D/S gradually decreased from latency to the second stage of labor,and increased postpartum,the comparison was statistically significant( P ＜0.05 ).(3) Comparison of cardiac reserve function in uterine contractions of each stage of labor:① Maternal heart rate gradually decreased from latent stage of labor to the second stage of labor,the comparison was statistically significant( P ＜ 0.05 ) ;② The ratio of S1/S2 of maternal gradually increased from latent stage of labor to the second stage of labor,the comparison was statistically significant( P ＜ 0.05 ) ; ③ The ratio of D/S gradually decreased from latency to the second stage of labor,the comparison was statistically significant (P ＜0.05).Conclusions The maternal cardiac reserve function decreased in uterine contractions than relaxation during labor; With the progress of labor,the maternal cardiac reserve function declined,especially in the second stage of labor,and recovered in postpartum stage.

Objective To observe the degradation pattern of tubulin in the cardiac muscle and lung tissue of rats after death.Methods 24 young male Sprague-Dawley rats were divided into 6 groups averagely.After being killed by bleeding,the rats were kept in an artificial climate incubator;the experimental temperature was kept at 20℃ and humidity at 50%.Proteins in cardiac muscle and lung tissue were extracted and tubulin was then examined by Western blot.Thereafter,the semi-quantitative analysis of the Western blot images was performed.Results Tubulin can be detected in the normal cardiac muscle and lung tissue of rats.After 1d postmortem,the tubulin content in these tissues began to decline,and it might still be detected at 7d postmortem,but the tubulin content in lung decreased faster than that in cardiac muscle.A linear relationship between tubulin content in cardiac muscle and lung of rats and the postmortem interval was determined.The linear regression equations and coefficients for integral optic density in relation to PMI were y=-1726.1x+14083 with correlative coefficient r=0.9684 for cardiac muscle and y=-1439.89x+12041 with correlative coefficient r=0.9808 for lung,respectively.Conclusion The level of tubulin in cardiac muscle and lung tissue of rats varied with PMI and the time dependent degradation of this protein was potentially useful for the PMI estimation.

Objective To observe the degradation of tubulin in liver,spleen and kidney of rats killed by bleeding and to explore its usefulness in estimation of postmortem interval(PMI).Methods 24 young male Sprague-Dawley rats were killed by bleeding and kept in an artificial climate incubator for 0,1,2,3,5 and 7d respectively,then the content of tubulin in spleen,liver,and kidney were examined by western-blot assay and the data of detection were analyzed statistically by using SPSS 11.5 software.Results Within a certain postmortem interval(PMI

Objective To find the chemical elements whose contents are abnormal in the soil of the high prevalence areas of birth defects in Shanxi Province. Methods 131 soil samples were collected from the high and low prevalence areas of birth defects respectively in 2005, the contents of 16 elements were determined with inductively coupled plasma (ICP)machine. The data were analyzed with regression and nonparametric tests. Results The total content of elements in the soil of the high prevalence areas was significantly higher than that of the low prevalence area. Compared with the low prevalence area, in a high prevalence area, the content of Mo, As, Pb, Ni, V and Se in soil was abnormal, Mo, As, Ni and Pb showed a significant relationship to the prevalence rate of birth defects, in another high prevalence area, the content of Sn, Se, Mo, Zn, Sr, Pb, Ni, Fe, V, Ca, Cu, Al, Mg and Na was abnormal, Pb, Mg, Ca, Al and Zn showed a significant relationship to the prevalence rate of birth defects. Conclusion Maybe the abnormal content of Mo, As, Ni, Pb, Mg, Ca, Al and Zn in the soil is one of the risk factors for birth defects.

Objective To develop a high performance liquid chromatography(HPLC)method for the determination of active components,sodium ascorbic phosphate,?-arbutin,hydroquinone,niacinamide,brillian-280 and phenol,in the whitening and Freckle-removing cosmetics.Methods Whitening and freckle-removing active components were separated on a C8 column(250 mm?4.6 mm,5 ?m)by using triethanolamine solution(11.15g triethanolamine dissolved in 1 L water,pH=7.7 adjusted with 1.75 ml H3PO4)+methanol=70+30 as the mobile phase at a flow rate of 1.2 ml/min,using diode array detector(DAD)with the detection wavelengh of 273 nm and the column temperature of 25℃.Results The coefficient of variation was less than 4.7% and the recovery rates were 87.3%-112.9%.Conclusion The method introduced in this paper is simple,rapid,accurate and is suitable for the analysis of various cosmetic samples.