1.The Effect of XiaoAiping on proliferation and apoptosis of hepatocellular carcinoma cells Hepa1-6
Heping ZHAO ; Yanru XIE ; Liqun LIANG
Cancer Research and Clinic 2010;22(7):476-478
Objective To study the effects of XiaoAiping (XAP) with different concentrations and action time on the proliferation and apoptosis of hepatocellular carcinoma cells Hepa1-6. Methods The hepatocellular carcinoma cell line Hepal-6 was treated with XAP at doses of 30 mg/ml(the high concentration group), 20 mg/ml (the moderate concentration group) and 10 mg/ml (the low concentration group) for 24 h, 48 h and 72 h, respectively. The MTT assay was used to detect the inhibitory effects of XAP on cell growth. The cell morphological alteration was observed after HE staining, and the apoptosis rates were assayed by flow cytometry. Results XAP produced an obvious time-and-dose-dependent inhibitory effect on the Hepa1-6 cells (P<0.01), and the cell differentiation trend was benign on light microscopy. After 48 h Hepa1-6 cells were incubated by XAP, there was an apoptosis peak, and the apoptosis rate was increased statistically in XAP group with the increasing XAP concentration [(7.65±0.40)%, (11.26±1.09)% and (26.71±0.85)% in low, moderate and high concentration group, respectively]compared with that in the controls (2.88 ±0.30)%, P < 0.01). Conclusion XAP produced obvious time-and-dose-dependent inhibitory effects on Hepa1-6 cells. Inhibiting DNA synthesize and inducing apoptosis of tumor cells may involve in the mechanisms of antineoplastic effect.
2.Treatment of vascular Parkinson's syndrome after stroke by ultralow frequency and high frequency repetitive transcranial magnetic stimulation.
Renming XIE ; Yanru LI ; Da LEI
Journal of Central South University(Medical Sciences) 2015;40(4):351-355
OBJECTIVE:
To determine effect and safety of ultra-low frequency and high frequency repetitive transcranial magnetic stimulation on treating vascular Parkinson's syndrome (VPS) after stroke.
METHODS:
The 0.1 Hz low frequency (n=21) and 5 Hz high frequency (n=21) rTMS were used to treat patients with VPS, and the false stimulation servered as a control group (n=18). The UPDRS score and Parkinson's Disease Questionnaire (PDQ) were chosen to evaluate the curative effect on PD. The patients were given anti-PD drugs continuously during the treatment.
RESULTS:
UPDRS scores as well as I, II, and III scores after the treatment were significantly decreased in both the ultra-low frequency group and the high frequency group compared with those before the treatment (all P<0.05). The scores at the third month after the treatment were still lower than those before the treatment (P<0.05), but there was no significant difference between the ultra-low frequency and the high frequency group at the same time point before and after the treatment (P>0.05). There was no significant difference in UPDRS scores between before and after the treatment in the control group (P>0.05), but PDQ scores were significantly decreased at the third month after the treatment compared with those of before and after treatment (P<0.05).
CONCLUSION
The low frequency and high frequency repetitive transcranial magnetic stimulation can safely improve the clinical symptoms and life quality of patients with VPS.
Humans
;
Parkinson Disease
;
therapy
;
Quality of Life
;
Stroke
;
complications
;
Transcranial Magnetic Stimulation
;
Treatment Outcome
3.Prospective and comparative study of the double eyelid blepharoplasty with central minimal incision and with three minimal incisions.
Ming LI ; Yide XIE ; Yakuan ZHOU ; Mingkun ZHAN ; Limin WANG ; Yanru CHERN ; Yongnian GUO
Chinese Journal of Plastic Surgery 2014;30(6):409-413
OBJECTIVETo compare the clinical effect of the double eyelid blepharoplasty with central minimal incision and with three minimal incisions. Methods: From Jul. 2010 to May 2012, 268 cases (Group A) received double eyelid blepharoplasty with central minimal incision, while 102 cases (Group B) underwent double eyelid blepharoplasty with three minimal incisions. Photos were taken immediately, and 1,2, 4, 8,12 weeks after operation. Operation time, recovery time and postoperative complications were evaluated and recorded. The operation time and recovery time were analyzed by Wilcoxon rank sum test. The postoperative complications were analyzed by chi square test. The satisfactory degree was analyzed by t test.
RESULTSThe operation time in Group A was (25.63 ± 3.74) min, compared with that (29.90 ± 4.13) min in Group B (Z = -8.011, P <0.01). Meanwhile, the recovery time in Group A was shorter than that in Group B (Z = -15.887, P <0.01). The occurrence rate of postoperative complications,including hematoma,recurrence and scar hyperplasia in Group A was also lower than that in Group B. At the same time, the satisfactory degree in Group A was(97.302 ± 1.764), which was higher than that(88.628 10.880) in Group B (t = 12.650, P <0.05).
CONCLUSIONSThe double eyelid blepharoplasty with central minimal incision, which is suitable for all cases except those who has serious blepharochalsais, has more advantages than double eyelid blepharoplasty with three minimal incisions.
Blepharoplasty ; adverse effects ; methods ; Cicatrix ; pathology ; Eyelids ; surgery ; Hematoma ; Humans ; Hyperplasia ; Photography ; Postoperative Complications ; Prospective Studies ; Recurrence
4.The effects of external treatment with honey for acute cellulitis on local symptoms and C-reactive protein
Peizhu XIE ; Caiping CHENG ; Jieying ZHOU ; Shaofang LI ; Haiyun LU ; Yanru LI
Chinese Journal of Practical Nursing 2016;32(21):1619-1622
group was (1.20 ± 1.09),(1.09 ± 1.04), (0.86 ± 0.42), (1.89 ± 1.30) and that of the control group was (2.40 ± 1.87), (2.34 ± 1.71), (1.60 ± 1.33),(3.49 ± 1.72) respectively, which had statistical significance between the two groups (t=3.139-4.390, P<0.01). the Blood CRP values and CRP positive rate in the observation group, were (6.34±2.74) and 17.14%(6/35) respectively and which of the control group were (10.35±7.37) and 42.86%(15/35), the difference of the two groups was statistically significant (t=2.881, P<0.01 andχ2= 5.250, P < 0.05). Conclusions Topical treatment of acute cellulitis with honey can effectively improve the clinical symptoms, reduce the level of c-reactive protein, which is better than magnesium sulfate wet compress.
5. Effect of long-term low-concentration mixed benzene exposure on male peripheral blood in an automobile manufacturing enterprise
Yanru LI ; Chunjiao XIE ; Congxi QIU ; Qiuyue LIN ; Yimin LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(2):119-121
Objective:
To investigated the effect of long
6.Relationship between sense of coherence and coping styles in patients with peritoneal dialysis
Li ZHANG ; Yun CHEN ; Yi ZHANG ; Xuefeng XIE ; Huiqun LI ; Yanru CHEN ; Wen XIE
Modern Clinical Nursing 2017;16(9):35-40
Objective To explore the relationship between sense of coherence and coping styles among patients at the stabile phase after peritoneal dialysis. Methods About 86 patients at stable phase after peritoneal dialysis were recruited in the investigation. The sense of coherence (SOC-13) and medical coping modes questionnaire (MCMQ) were used to evaluate the sense of coherence and coping styles. Results The average score of sense of coherence was (60.93±13.77), which was at a lower level. The scores of coping styles were confrontation, avoidance and resignation, ranked from high to low. Avoidance and resignation dimensions were higher than the norm in coping styles (all P<0.001) and there was no statistically significant difference between confrontation dimension and the norm (P>0.05). There was positive relationship between confrontation and the average score of sense of coherence, comprehensibility, manageability, meaningfulness (all P<0.05). There was negative relationship between resignation and the average score of sense of coherence, comprehensibility, manageability, meaningfulness (all P<0.001). Conclusions Patients at the stable phase after peritoneal dialysis have a lower sense of coherence and are inclined to confrontation and avoidance coping styles. The higher sense of coherence, the more positive coping styles PD patients use. Medical workers should provide personal interventions according to the results of the sense of coherence so that the patients take positive coping styles.
7.Study on the relationship between HSP70 polymorphism and susceptibility to occupational noise-induced hearing loss
Chunjiao XIE ; Yanru LI ; Lili HUANG ; Yanhua LI ; Yingmei LIU ; Huifen FU ; Zhi WANG
China Occupational Medicine 2024;51(1):25-30
ObjectiveTo investigate the correlation between genetic polymorphism of heat shock proteins 70 (HSP70) and susceptibility to occupational noise-induced hearing loss (ONIHL). Methods A total of 229 ONIHL workers were selected as the case group and 229 healthy workers with similar age, years of noise exposure, and noise exposure levels were selected as the control group using the case-control study method. Occupational health examinations were conducted on both groups, and peripheral blood of individuals was collected for DNA extraction. The genotypes of three single nucleotide polymorphisms of the HSP70 were detected using the MassArray system. Results The allele frequency distribution of HSP70 rs2227956, rs1043618, and rs1061581 in the control group was in Hardy-Weinberg equilibrium (all P>0.05). The genotype and allele frequency distribution of rs2227956 was significantly different between the two groups (all P<0.05), while no significant difference was found for rs1043618 and rs1061581 (all P>0.05). After adjusting for age, years of noise exposure, individual noise exposure level, smoking, and drinking, individuals with AG and AG+GG genotypes of rs2227956 had a higher risk of ONIHL than those with AA genotype (all P<0.05). The risk of ONIHL was higher in individuals with G allele of rs2227956 than in those with A allele (P<0.05). No correlation was found between rs1043618 and rs1061581 polymorphisms and the risk of ONIHL (all P>0.05). Conclusion The rs2227956 polymorphism of the HSP70 gene is correlated with susceptibility to ONIHL in noise-exposed workers, and the G allele is a risk factor for ONIHL in this population.
8. Analysis of common gynecological diseases in 1142 married female workers
Yanru LI ; Yongxiang TANG ; Congxi QIU ; Qiuyue LIN ; Chunjiao XIE ; Muying ZHOU ; Yimin LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(10):785-788
Objective:
To understand the prevalence of gynecologic diseases among married female workers.
Methods:
The data of married female workers who underwent occupational health examination in a physical examination center from January to December 2017 were collected. The relationship between the detection of common gynecological diseases, age and occupational types examined by gynecological routine, TCT, breast B-mode ultrasound, uterus and appendix B-mode ultrasound were analyzed.
Results:
Among the 1142 female workers, the total detection rate of reproductive tract infections was 67.25% (768/1142), the total detection rate of breast-related diseases was 75.22% (859/1142) ; the total detection rate of gynecological tumors and benign lesions was 14.71% (168/1142). The detection rate of breast hyperplasia was the highest 67.08% (766/1142), followed by vaginitis 51.66% (590/1142). Among the abnormalities detected in breast-related diseases, gynecological tumors and benign lesions, the highest detection rate was found in public institutions (85.66% and 27.13%), and the lowest was found in factory workers (70.24% and 7.89%). With the increase of age, the detection rate of breastrelated diseases (breast hyperplasia, breast cyst), gynecological tumors, benign lesions (uterine myoma), and Nessler's cyst abnormalities in married female workers increased (χ2trend=7.647、21.653、107.411、53.802,