Fourty-five Cases of piriformis syndrome were treated by acupuncturing Huantiao(GB 30),Yanglingquan (GB 34) and Kuanlun (BL 60). After 20 treatments,36 individuals were well cured and 9 were effective.

Objective To investigate the evaluation of cardiopulmonary exercise test (CPET) in the patients with chronic obstructive pulmonary disease (COPD), and the relationship with the pulmonary function test (PFT). Methods 26 COPD patients and 30 normal controls were examined with CPET and PFT. Results The parameters of CPET, including maximum oxygen uptake (VO2max), anaerobic threshold (AT), maximum minute ventilation (VEmax) and breathing reserve (BR) were lower in COPD group than those in control group (P<0.05). There were no significant differences in Wmax, oxygen pulse (O2pulse) between two groups (P>0.05). VO2max, AT, VEmax and BR were positively correlated with forced expiratory volume in one second (FEV1) and peak expiratory flow (PEF) in COPD group （P<0.05）, but were not correlated with forced vital capacity (FVC) and FEV1/FVC (P>0.05).Conclusion The pulmonary function of COPD patients can be evaluated with CPET, especially VO2max, AT, VEmax and BR.

Objective The aim of this study is to evaluate clinical application of BODE (body mass index, airflow obstruction, dyspnea, and exercise capacity) index in patients with chronic obstructive pulmonary disease (COPD). Methods Forty patients with stable COPD in Xuanwu Hospital of Beijing during September 2003 to December 2007, were recruited in the study, including 25 men and 15 women, aged 38 to 85 years (with a mean of 62 ± 13 years). BODE index was measured for each patient, one year and three years after recruitment, respectively. Severity of COPD, including number and duration of episodes, length of each hospital stay and times of hospitalization due to acute exacerbation of COPD (AECOPD), were recorded. All data were statistically analyzed with SPSS 13.0 software for windows. Results ①There was a remarkable difference in COPD severity between patients with BODE score equal to or greater than five and these with less than five (P <0.01 ). ①BODE score inversely correlated with forced expired volume at the first second ( FEV1 ) in COPD patients (P < 0.01 for those with BODE score equal to or greater than five and P <0.05 for these with BODE score less than five, respectively). ③BODE score positively correlated with number and duration of acute exacerbation, length of hospital stay and times of hospitalization due to AECOPD (P <0. 01 for those with BODE score equal to or greater than five and P < 0. 05 for those with BODE score less than five, respectively). For patients with COPD, their BODE score in the first year positively correlated with that in the third year ( r = 0.834, P < 0.01 ). Conclusions BODE index is a better indicator to evaluate their condition severity in patients with COPD.

To ascertain whether heat shock gene expression could protect pulmonaryendothelial cell from hydrogen peroxide(H2O2)一indueed injury,the protective effect of HSPgene expression induced by pretreatment of bovine pulmonary endothelial eells(BPAECs)by heat shock (42 ℃, 2h)against lethal dose(lmmol?L(-1),45min) of H2O2一induced cyt-otoxieity was observed in vitro.It was found that BPAECs heat一shocked prior to exposureto H2O2(Immol?L(-1) 45min)showed significant decrease in H2O2一mediated incrementof LDH rdlease and TBARS production and had an obvious alleviation of H2O2一induccddecreased activities of catalase and superoxide dismutase. Further study showed thatcycloheximide, a protein synthesis inhibitor and Actinomycin D,a mRNA transcriptioninhibitor blocked the expression of HSP 70 and HSP 70 mRNA respectively.Both agentsprevented the cytoprotective effect of heat shock pretreatment against H2O2一mediatedBPAECs injury. The results suggested that HSP70 gene selectively translated after heat shockwas invoived in enhancement of eellular antioxidant mechanism and protected BPAECsagainst H2O2一induced injury

Objective To investigate the changes in the expression of mitochondrial transcription factor A (mtTFA) during one-lung ventilation (OLV)-induced lung injury in rabbits.Methods Sixty healthy male New Zealand white rabbits,weighing 2.5-3.0 kg,were randomized into 2 groups (n =8 each):two-lung ventilation (TLV) group and OLV group.The animals were anesthetized with iv 3％ pentobarbital sodium 30 mg/kg and tracheostomized.A self-made double lumen catheter was then intubated.Bilateral lungs were ventilated for 3 h in group TLN.In group OLV the left lung was ventilated for 2 h followed by 1 h TLV.Arterial blood samples were taken for blood gas analysis immediately after the beginning of ventilation,at 1 and 2 h of ventilation,and immediately after the end of ventilation.The oxygenation index was calculated.The animals were sacrificed after the end of ventilation and the apex of the left lung was removed and then cut and stained with HE for microscopic examination.The pathological changes of the lung were scored.The expression of mtTFA in lung tissues was measured by Western blot.Results Oxygenation index was significantly decreased,lung injury score was increased,the expression of mtTFA was down-regulated in group OLV compared with group TLV (P ＜ 0.05).The pathological changes of the lung were aggravated in group OLV.Conclusion OLV induces lung injury by down-regulation of mtTFA expression in rabbit lung tissues.

Objective To explore the value of retinoblastoma binding protein 4 ( RBBP4 ) in diagnosing prostate cancer ( PCa).Methods From January 2015 to December 2015, the prostate tissue after prostatectomy were collected and the differentially expressed degree of RBBP4 protein was analyzed in PCa and adjacent tissues by 2D-DIGE technology.The RBBP4 score of prostate tissue chip which contains 3 normal prostate tissues, 7 cancer adjacent normal prostate tissues, 50 adenocarcinoma and 20 hyperplasia tissue was checked by immunohistochemistry( IHC).In 50 patients with PCa, 4 cases were less than 60 years old and 46 cases were more than 60 years.In those patients, the Gleason scores were less than 7 scores in 18 cases, and more than 7 scores in 30 cases.22 cases were confirmed less than Ⅱ stage, and 28 cases were confirmed more than Ⅲ stage.Finally, the RBBP4 IHC score and the clinic-pathological parameters such as age, Gleason score and clinical stage of PCa patients were analyzed together.Results We found that the protein of RBBP4 increased by 2.15 times in PCa tissues compared to adjacent tissues by using 2D-DIGE technology( P=0.008).The expression of RBBP4 was higher than that in benign tissues by IHC ( F=43.972,P=0.000).And the expression of RBBP4 was positive correlation with Gleason score( t=5.589, P=0.000) and clinical stage(t=5.620,P=0.000), but was negative correlation with age(t=1.125,P=0.266).Conclusions The detection of RBBP4 can help to separate PCa from benign tissues.The overexpression of RBBP4 might result in the rapid growth of malignant cells.It may have certain value in determine the clinical staging and pathological grading of PCa.

Objective To construct a Nomogram model that can accurately predict early death of metastatic colon cancer (mCC). Methods A total of 6 669 patients from the SEER database were identified using inclusion and exclusion criteria. Multivariate logistic regression was used to identify risk factors for early mortality and to construct a Nomogram. The predictive performance of the Nomogram was evaluated by C-index, calibration curve, and decision curve analysis (DCA). Results Primary tumor location, differentiation grade, T stage, M stage, bone metastases, brain metastases, CEA, tumor size, age and marital status were independent factors for early death in patients with mCC. A Nomogram was constructed based on these variables. The C-index and the calibration curve of the Nomogram showed the good predictive ability of the nomogram. DCA showed that the Nomogram had a superior clinical net benefit in predicting early death compared with TNM stage. Conclusion The developed Nomogram has good predictive ability and can help guide clinicians to identify patients with high-risk mCC for individualized diagnosis and treatment.

Objective To investigate the efficacy of Saccharomyces boulardii combined with ur-sodeoxycholic acid in the treating rectal ulcerative colitis(UC)and its influence on intestinal mucosal barrier function.Methods A total of 88 patients with rectal UC were randomly divided into observa-tion group and control group,with 44 cases in each group.The scores of main symptoms(abdominal pain,diarrhea,purulent stool),score of the Inflammatory Bowel Disease Questionnaire(IBDQ),lev-els of inflammatory factor indicators[interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)]and levels of intestinal mucosal barrier function indicators[the ratio of urinary lac-tolic acid to mannitol(L/M),serum D-lactic acid(D-LA),serum diamine oxidase(DAO),and ser-um lipopolysaccharide(LPS)]were compared between the two groups before and after treatment.The incidence of adverse reactions were compared between two groups.Results After treatment,the scores of abdominal pain,diarrhea and purulent stool in both groups were significantly lower than those before treatment,while the IBDQ score was significantly higher than that before treatment,and the scores of abdominal pain,diarrhea and purulent stool in the observation group were significantly lower than those in the control group,while the IBDQ score was significantly higher than that in the control group(P＜0.05).After treatment,the levels of serum D-LA,DAO,LPS and urinary L/M in both groups reduced significantly,and the levels of these indicators in the observation group were signifi-cantly lower than those in the control group(P＜0.05).After treatment,the levels of serum IL-6,TNF-α and IL-1β in both groups reduced significantly,and the levels of these indicators in the observation group were significantly lower than those in the control group(P＜0.05).The total ef-fective rate inthe observation group was 86.36％,which was significantly higher than 68.18％in the control group(P＜0.05).There were no significant differences in incidence rates of adverse re-actions such as lower abdominal pain,allergies and refractory constipation between two groups(P＞0.05).Conclusion Saccharomyces boulardii combined with ursodeoxycholic acid is effective in the treatment of patients with rectal UC,which can effectively improve symptoms such as abdominal pain,diarrhea and purulent stool,inhibit the inflammatory response of the body,and alleviate dam-age to the intestinal mucosal barrier.

Objective To investigate the efficacy of Saccharomyces boulardii combined with ur-sodeoxycholic acid in the treating rectal ulcerative colitis(UC)and its influence on intestinal mucosal barrier function.Methods A total of 88 patients with rectal UC were randomly divided into observa-tion group and control group,with 44 cases in each group.The scores of main symptoms(abdominal pain,diarrhea,purulent stool),score of the Inflammatory Bowel Disease Questionnaire(IBDQ),lev-els of inflammatory factor indicators[interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)]and levels of intestinal mucosal barrier function indicators[the ratio of urinary lac-tolic acid to mannitol(L/M),serum D-lactic acid(D-LA),serum diamine oxidase(DAO),and ser-um lipopolysaccharide(LPS)]were compared between the two groups before and after treatment.The incidence of adverse reactions were compared between two groups.Results After treatment,the scores of abdominal pain,diarrhea and purulent stool in both groups were significantly lower than those before treatment,while the IBDQ score was significantly higher than that before treatment,and the scores of abdominal pain,diarrhea and purulent stool in the observation group were significantly lower than those in the control group,while the IBDQ score was significantly higher than that in the control group(P＜0.05).After treatment,the levels of serum D-LA,DAO,LPS and urinary L/M in both groups reduced significantly,and the levels of these indicators in the observation group were signifi-cantly lower than those in the control group(P＜0.05).After treatment,the levels of serum IL-6,TNF-α and IL-1β in both groups reduced significantly,and the levels of these indicators in the observation group were significantly lower than those in the control group(P＜0.05).The total ef-fective rate inthe observation group was 86.36％,which was significantly higher than 68.18％in the control group(P＜0.05).There were no significant differences in incidence rates of adverse re-actions such as lower abdominal pain,allergies and refractory constipation between two groups(P＞0.05).Conclusion Saccharomyces boulardii combined with ursodeoxycholic acid is effective in the treatment of patients with rectal UC,which can effectively improve symptoms such as abdominal pain,diarrhea and purulent stool,inhibit the inflammatory response of the body,and alleviate dam-age to the intestinal mucosal barrier.

Objective To investigate the effect of human caring ability on job satisfaction of nurses in the oncological department. Method About 215 nurses in the department were enrolled in the study with both caring nurse-patient action scale (CNPI-23) and Minnesota satisfaction questionnaire (MSQ). Results CNPI-23 score on oncology nurses was 88.42±13.54 and MSQ score 44.87±11.34. Nurses' caring ability was associated with age, work experience, marital status and job title. Nurses' humanistic care ability was positively correlated with job satisfaction (r=.433;P<0.001). Job satisfaction was positively related with humanistic care ability (t=6.617, P<0.001), which explained 23.4% variable. Conclusions The oncological nurses' caring ability needs to be improved. It is possible to improve the capability of nurse humanity from the perspective of job satisfaction.