Objective To provide the anatomic basis of transferring the lobe of parotid gland duct for the treatment of dry eye disease. Methods The first interlobar parotid gland duct and zygomatic branch of facial nerve were dissected and observed on parotid gland region of head and face of 5 (10 sides) of adult head specimens. the measurement data was recorded. Results The length of first interlobar duct of superficial parotid was (37. 51±1. 23) mm, the outside diameter of injected parotid duct was (0. 53±0. 15) mm. There was a close ana-tomical relationship between zygomatic branch of facial nerve and first interlobar duct of parotid gland. Conclusion the parotid interlobar duct transposition operation has the reference value in treatment of dry eye desease.