Objective To establish an HPLC method for the determination of Aloeemodin,Rhein,Chrysophanol,Emodin and Physcione in Huangxiong Kangshuan Capsule.Methods The determination was performed on Kromasil-C18 column(250 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-0.1% phosphoric acid(85:15) at a flow rate of 1.0 mL/min.Column temperature was 25 ℃.The UV detection wavelength was set at 254 nm.Results The calibration curves were linear in the range of 0.230～3.68 ?g(r=0.999 8) for Aloeemodin,0.224～3.584 ?g(r=0.999 9) for Rhein,0.223～3.568 ?g(r= 0.999 8) for Emodin,0.257～4.112 ?g(r=0.999 9) for Chrysophanol and 0.287～4.592 ?g(r=0.999 8) for Physcion.The average recovery was 98.48%,98.10%,99.08%,100.34%,97.52% and RSD was 1.39%,1.54%,1.44%,2.40%,2.03% respectively.Conclusion The determination method is simple,reliable,accurate,and it can be used for quality control of Huangxiong Kangshuan Capsule.

Objective A recent study has shown the association between a sulfonylurea receptor gene 1 (SUR1) variant and hyperinsulinemia in normal individuals from a high diabetes risk ethnic group,supporting the hypothesis that the primary insulin hypersecretion may be an antecedent of type 2 diabetes.Methods To test this hypothesis in Chinese population,we studied the allele and genotype distribution of the polymorphism at -3 position of intron 24 in SUR1 by PCR RFLP technique in 206 unrelated normal glucose tolerant subjects with strong family history of type 2 diabetes (group A) and 110 normal individuals without family history of diabetes (group B).Results The frequency of “-3c” allele and “-3cc” genotype of intron 24 in group A was significantly higher than that in group B (64% vs 54%, P =0 004 and 38% vs 24%, P =0.002 respectively).Moreover,in group A, those carrying “cc” genetype had a higher BMI (27 27?6 37 vs 24.99?3.43kg/m 2, P

Objective　A recent study has shown the association between a sulfonylurea receptor gene-1 (SUR1) variant and hyperinsulinemia in normal individuals from a high diabetes risk ethnic group,supporting the hypothesis that the primary insulin hypersecretion may be an antecedent of type 2 diabetes.Methods　To test this hypothesis in Chinese population,we studied the allele and genotype distribution of the polymorphism at -3 position of intron 24 in SUR1 by PCR-RFLP technique in 206 unrelated normal glucose tolerant subjects with strong family history of type 2 diabetes (group A) and 110 normal individuals without family history of diabetes (group B).Results　The frequency of “-3c” allele and “-3cc” genotype of intron 24 in group A was significantly higher than that in group B (64% vs 54%，P=0.004 and 38% vs 24%，P=0.002 respectively).Moreover,in group A, those carrying “cc” genetype had a higher BMI (27.27±6.37 vs 24.99±3.43kg/m2，P＜0.05；27.27±6.37 vs 25.28±2.78kg/m2，P＜0.05),fasting insulin (15.52±10.72 vs 9.27±5.03U/ml,P＜0.01；15.52±10.72 vs 10.79±7.80U/ml,P＜0.05) and 2h insulin levels (76.41±54.02 vs 55.43±49.60U/ml,P＜0.01；76.41±54.02 vs 55.71±40.39, P＜0.05) as well as lower insulin sensitivity ［HOMA(Ri］: 4.00±3.09 vs 2.79±1.32, P＜0.01； 4.00±3.09 vs 2.82±2.94, P＜0.01) as compared with that in carriers of other genotypes (“ct” and “tt”).Conclusion　This study suggested the possibility that the defect in SUR1 gene might contribute to the insulin hypersecretion which might be the cause of subsequent increased body weight and decreased insulin sensitivity.

Objective To study the immunomodulatory property of Compound Shougong powder(SGS) on S180 tumor-bearing mice.Methods The S180 model of tumor-bearing mice by Kunming mice were prepared.Then,the tumor-bearing mice were randomized into the model group,positive control group,and SGS groups (29.25g/kg,19.50g/kg,9.75g/kg).Besides the normal group,the other groups were used intragastric administration of into tumor-bearing mice by one time,14 consecutive days.The carbon clearance,quantitative hemolysis and DNFB induced delayed-type hypersensitivity were applied to assay effects of SGS on nonspecific immunity,humoral immunity and cellular immunity.Results Compared with the model control group,in the SGS groups,the tumor-bearing mice with spleen and thymus index of organ improved (all P ＜ 0.05),and the clearance index and values of phagocytic index were elevated(all P ＜0.05),and the productions of IgM and IgG in serum and hemolysin in splenocytes were enhanced(all P ＜0.05),and the percentages of T cells expressing CD4+,CD8+ and the ratio of two subset of T lymphocyte increased,and the IL-2 production of spleen lymphocytes was also improved (all P ＜ 0.05).Conclusion SGS showed significant immunomodulatory property on tumor-bearing mice through specific and nonspecific immunity.

Objective To study the best processing technology of Xanthii Fructus by determining the contents of chlorogenic acid and 3,5-dicaffeoylquinic acid in which processed by different temperature and time. Methods Sixteen batchs samples of Xanthii Fructus were propressed by stir-frying with sand, and the propressed temperature and time were set at 150-220 ℃ and 0.5-7 minutes. Two phenolic acid components in Xanthii Fructus were simultaneously determined. The column was UPLC Acquity BEH C18 (2.1 mm×100 mm, 1.7 μm). The mobile phase was acetonitrile-0.1% phosphoric acid, gradient elution. The flow rate was 0.25 mL/min, and the detection wavelength was 327 nm. Results The sample with highest contents of chlorogenic acid and 3,5-dicaffeoylquinic acid was the batch processed by stir-frying with sand at 160 ℃ for 7 minute, which was 2.498, 2.004 mg/g, respectively. Conclusion According to the appearance of processed sample and the content of chlorogenic acid and 3,5-dicaffeoylquinic acid, the optimal processing technology of Xanthii Fructus was stir-frying with sand at 160 ℃ for 7 min.