Objective To observe the effect of Fuzheng Hunao capsule on TNF-? and HSP70 protein expression of myocardial tissues in rats with intracerebral hemorrhage.Methods Seventy two rats were randomly divided into 4 groups:sham,model,Fuzheng Hunao and Angong Niuhuang.The protein expression of TNF-? and HSP70 in rat myocardial tissues were detected by the immunohistochemistiy method,and the integrated optical density (IOD) was measured with image analysis.Result The IOD of TNF-? and HSP70 in model group was higher than that in sham group.The IOD of TNF-? in Fuzheng Hunao and Angong Niuhuang group was lower than that in model group.While the IOD of HSP70 was higher than that in model group.Conclusion Inflammatory response attend the myocardial damage.Fuzheng Hunao capsule could protect the myocardial tissues by inhibiting the protein expression of TNF-? and increasing that of HSP70.

Objective To evaluate the effects of sevoflurane preconditioning on caveolin-3 expression during myocardial ischemia-reperfusion (I/R) in rats.Methods Healthy male Wistar rats,aged 6-8 weeks,weighing 250-300 g,were anesthetized with intraperitoneal pentobarbital 30 mg/kg and heparin 1 000 IU/kg.Their hearts were excised and perfused with K-H solution in a Langendorff apparatus.Thirty isolated rat hearts were randomly assigned into 3 groups (n =10 each) using a random number table:control group (group C),I/R group and sevoflurane preconditioning group (group SP).After 30 min of equilibration,group C was continuously perfused with K-H solution for 90 min,group I/R underwent 30 min of ischemia followed by 60 min of reperfusion,and group SP was perfused with K-H solution saturated with 2％ sevoflurane for 10 min followed by 5 min washout with K-H solution,then underwent 30 min of ischemia followed by 60 min of reperfusion.HR,left ventricular enddiastolic pressure (LVEDP),+ dp/dtmax and-dp/dtmax were recorded at the end of equilibration,immediately before ischemia and at 30 and 60 min of reperfusion.Myocardial specimens were obtained from the cardiac apex for microscopic examination.Myocardial specimens were obtained from the left ventricle for determination of caveolin3 expression.Results Compared with group C,+ dp/dtmax and-dp/dtmax were significantly decreased immediately before ischemia,and HR,LVDEP,+ dp/dtmax and-dp/dtmax were decreased at 30 and 60 min of reperfusion in groups I/R and SP,and caveolin-3 expression was down-regulated in group I/R and up-regulated in group SP (P ＜ 0.05).Compared with group I/R,HR,LVDEP,+ dp/dtmax and-dp/dtmax were significantly decreased immediately before ischemia and increased at 30 and 60 min of reperfusion,and caveolin-3 expression was up-regulated in group SP (P ＜ 0.05).The pathological changes were significantly attenuated in group SP as compared with group I/R.Conclusion The mechanism by which sevoflurane preconditioning attenuates myocardial I/R injury in rats may be related to up-regulation of myocardial caveolin-3 expression.

Objective To investigate the antitumor mechanism of sodium arsenite on human gastric carcinoma cell line SGC-7901 in vitro. Methods MTT assay, light microscopy, electron microscopy, flow cytometry, and immunocytochemical staining were used to analyze the effect of sodium arsenite on biologic behavior of SGC-7901 cells. Results Sodium arsenite (2.50 ～ 40.00 ?mol/L) could inhibit the growth of gastric carcinoma cells, it depended on the duxation and concentration, and its 50% inhibitory concentration(IC50) was 8.69 ?mol/L after 72 hours' treatment. SGC-7901 cells were arrested significantly in G2/M phase treated with sodium arsenite for 48 and 72 hours. SGC-7901 cells presented typical morphologic feature of apoptosis and necrosis after exposure to sodium arsenite. Sodium arsenite up-regulated Caspase-3 protein expression in SGC-7901. Conclusion Sodium arsenite could obviously inhibit the proliferation of SGC-7901 cells, induce cell cycle arrest and apoptosis and necrosis of the cells. its mechanism is possibly associated with inhibition of elimination of ROS and the up-regulated expression of Caspase-3 protein.

In recent years, there's a new theory of lipid condensation or accumulation being the etiology for obstruction of qi in the chest result in heart disease. The theory believes that thick lipid exists in meridians. If one takes too much fatty diet, there will be lipid condensation or accumulation, resulting in disturbance of qi transformation in meridians,. Scholars explore the relationship of preventive treatment of disease by traditional Chinese medicine with lipid condensation or accumulation theory. This enriches the etiology of TCM.

Objective To observe the effect of Fuzhenghunao capsule on NE of the locus ceruleus and myocardial tissues in rats with intracerebral hemorrhage. Methods 72 rats were randomly divided into 4 groups:sham,model,Fuzhenghunao and Angongniuhuang. The content of NE were tested by the high performance liquid phase-electrochemical method at 6 h,24 h and 72 h of post-operation. Result NE was obviously increased in sham group,and decreased in two treatment groups. Conclusion Fuzhenghunao capsule could be used to inhibit the activation of locus ceruleus noradrenalin system and protect myocardial tissues by regulating NE content.

AIM:To explore clinical effect of Zhongfeng Fangzhiling Granule(Radix Pseudostellariae,Radix Polygoni Multiflori praeparata cum succo glycines sotae,Hirudo,etc.) on acute cerebral thrombosis.METHODS:72 patients were randomly divided into 2 groups.The treatment group was treated with Zhongfeng Fangzhiling Granule,the control group was treated with Zhilong Xuetong Capsule.The treatment effects between two groups were compared.RESULTS:The effect in treatment group was superior to that in control group.CONCLUSION:Zhongfeng Fangzhiling Granule has significiant therapeutic effect on patients with acute cerebral thrombosis,and the preparation method is simple,so it will be a prospective herb granule.

Objective:To study the feasibility of repair of partial maxillary defect by distraction osteogenesis(DO) in zygoma.Methods:Segmental maxilla- zygoma defect was made in 3 dogs. A submerged DO distractor was anchoraged on the zygoma and maxilla across the defect,the zygoma was distracted with a rate of 1.0 mm/d for ten days,the process of bone formation was examined by X-ray.Results:All dogs were in health condition within the experimental period.New bone formation was observed 2~4 weeks after operation. Complete repair of the defects was observed 8 weeks after the completion of distraction.Conclusion:The partial maxillary defect may be repaired by DO in zygoma.

BACKGROUND: The rehabilitation of maxillary loss is based on maxillary prosthesis,and the most difficult problem is that retention and support of maxillary prosthesis can hardly be achieved. The employment of implants in the zygoma region provides good basis for the retention and support of maxillary prosthesis. However,during implantation in the zygoma the pe ripheral tissues may be injured, and even induce failed implantation.OBJECTIVE: To study the bone structure of the zygoma so as to provide reference for implantation in this region.DESIGN:Randomized sampling for repeated observation measurement based on maxilla specimens.SETTING: The research was completed in the prosthodontic department of stomatological college and department of human anatomy of a military medical university.PARTICIPANTS:The experiment was carried out in the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA from March to May 2002. A total of 73 adult maxilla specimens(38 from males and 35 from females) were provided by the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA.METHODS: Quantitative measurement of bone structure of the zygoma region was made with the cursor ruler in the 73 specimens. T-test was used to analyze the measurement data.MAIN OUTCOME MEASURES: The data of measurement: bone thickness of the zygoma, width of the lower part of the zygoma, length of the zygoma, and the distance between lower part of the zygoma and orbital floor.RESULTS: ① Bone thickness gradually decreased from the interior-inferior borderline to the exterior-superior part of the zygoma. In males, the average bone thickness 10 mm,12 mm,14 mm and 16 mm away from the interior-inferior borderline of the zygoma was 6.97 mm, 6.45 mm, 5.75 mm and 5. 01 mm, respectively, in the upper part, and 7.03 mm, 6.69 mm, 6.28 mm and 5.89 mm in the lower part. In females, the average bone thickness 4 mm,6 mm, 8 mm and 10 mm away from the interior-inferior borderline was 6.95 mm,6. 55 mm,6.28 mm and 5.31 mm in the upper part,and 6.60 mm,6. 39 mm,6.26 mm and 5.75 mm in the lower part. ② The lower part of the zygoma was 21.39 mm wide in males and 17.20 mm in females. ③ The average length of the zygoma was 21. 12 mm in males and 19.58 mm in females. ④ The average distance between lower part of the zygoma and orbital floor was 4. 17 mm in males and 3.71 mm in females.CONCLUSION:In maxillary loss,the zygoma is an optimal region for double implants at a distance of more than 15 mm. In males, the implants of 3.6 -4.0 mm in diameter and 10 - 12 mm or 14 - 16 mm in lengthcan be employed; in females, the implants of 3.4 - 3.8 mm in diameter and 4 - 6 mm or 8- 10 mm in length can be employed.The differences in bone quantity between males and females are statistically significant in the zygoma,males' better than females', so they should be treated differently during clinical practice.

Objective To observe the impacts of lumbar plexus block combined with sciatic nerve block on postoperative cognitive function and plasma S100βlevel in eldly patients undergoing hip surgeries.Methods Sixty patients of ASA Ⅱ or Ⅲ aged 65-85 years,undergoing selective hip sur-geries,were randomly divided into 2 groups (n=30 each).Group G received general anesthesia with tracheal intubation,while group L received ultrasound-guided lumbar plexus block and parasacral sci-atic nerve block combined with general anesthesia.The mini-mental state examination (MMSE)score was recorded before anesthesia,1 d,3 d and 7 d after operation.The plasma S100βlevel was detected before anesthesia,1 h and 7 d after operation.Results The MMSE scores declined on 1 d after oper-ation in both groups(P <0.05),and the value was lower in group G(P <0.05).There were 18 (56.7%)patients diagnosed with POCD in group G at the first day postoperatively,while it was 10 (33.3%)in group L,and the difference was significant(P<0.05).The MMSE scores were still low-er at 3 d after operation (P<0.05).The concentrations of plasma S100βincreased in both groups at 1 h after operation,and it was more obviously in group G (P <0.05).Conclusion Lumbar plexus block combined with parasacral sciatic nerve block might reduce the stress reaction and dosages of general anesthetics in eldly patients undergoing hip surgeries,minimize the damages of central nervous system,protect the cognitive function and reduce the incidence of POCD.

Objective To prepare pemirolast potassium nasal spray which could be used in treatment of allergic rhinitis and make a quality control standard for the preparation.Methods The preparation materials of nasal spray was selected which could meet the physical and chemical properties of pemirolast potassium as well as the characteristics of intranasal administration.The quality control standards for pemirolast potassium nasal spray was made according to the nasal spray quality standards for the formulation pH, property, loading capacity, total bottle spray times, puff volume, sedimentation volume ratio and detection of content of “Chinese Pharmacopoeia” two section (2010 edition).Results The formulation prescription of pemirolast potassium nasal spray was:0.1% potassium pemirolast, 0.8% CMC-Na, 0.01% benzalkonium bromide, glacial acetic acid ( pH adjusting agent) and the solution was 5% mannitol.The formulation was a white suspension, pH=7.The mean value of installed capacity was (25.12 ±0.16) mL and installed capacity of each bottle was above 23.75 mL(95%); total spray times of each bottle was above 160 sprays and spray volume was above 0.140 0 g/press; sedimentation volume ratio was 1, the above testing programmes of quality standard met the requirements and were controlled.The standard curve of pemirolast potassium reference substance was Y=81085X+15264(r=0.999 8), linearity range was 0.5~25.0μg/mL.The average recovery rate of pemirolast potassium sample was 99.39%, and precision met the requirements.The pemirolast potassium content of test sample was 99.2% of labeled amount.Conclusion The formulation prescription of pemirolast potassium nasal spray is suitable and the preparation is possessed several advantages such as stability, well-distributed spray, application convenience and quality controlled.Therefore, nasal administrated formulation of pemirolast potassium is potentially in further.