Objective:To evaluate the influence on root resorption after distalizing molars with face bow.Methods:15 cases whose molars were distalized with face bow were selected in present research. Root lengths were measured on pre-treatment and post-treatment dental films taken with the oral positioner. The distances of molar distalization were measured on cephalometric radiographs.The differences of the pre-treatment and post-treatment root lengths were evaluated by the Paired t-test.The correlation between the distance of molars distalization and the root resorption of the maxillary molars was analyzed with Pearson's correlation coefficients.Results:The root lengths between the pre-treatment and post-treatment showed a statistically significant difference(P

Objective To analyze the vascular architecture characteristics of the complex direct cavernous arteriovenous fistula (cd-CAVF) and to discuss its treatment and the curative effect of interventional embolization. Methods The hospitalization records, imaging features and operation records of 12 patients with cd-CAVF were retrospectively analyzed. Results In the 12 patients with cd-CAVF, the lesion’s blood supply arteries included internal carotid artery (ICA,n=8), primary trigeminal artery (PTA,n=1), middle cerebral artery (MMA,n=2) and basilar artery (BA,n=1). Different degrees of “arterial steal” phenomenon could be observed in all patients. The drainage routes included the superior ophthalmic vein and the inferior petrosal sinus (n=10), and cortical vein (n=2). Interventional embolization was carried out via ICA (n=4), through both ICA and BA (n=5), through MMA (n=2), or through BA (n=1). For the embolization of the lesion the balloons were used in 8 patients, steel coils were adopted in 2 patients, and balloons together with coils were employed in 2 patients. All the patients were followed up for 3-6 months. After the treatment the clinical symptoms and signs disappeared, and the lesions were completely cured in all patients with no complications. During the follow-up period of (60.2 ±26.8) months no recurrence of CAVF was observed. Conclusion The blood supply of cd-CAVF comes directly from the rupture of the blood vessels surrounding the cavernous sinus wall, the “arterial steal” phenomenon is prone to occur, and the drainage via the superior ophthalmic vein and the inferior petrosal sinus is more often seen. Transarterial balloon embolization is very effective for the treatment of cd-CAVF, and the use of coils together with multi-artery approaches is an effective supplementary method.

We studied the epidemiological characteristics of human bocavirus 4 (HBoV4) in children with a- cute gastroenteritis in Lanzhou (China). A total of 331 stool specimens were collected from children aged < 5 years with acute diarrhea at the First Hospital of Lanzhou University between July 2012 and June 2013. Specimens of HBoV were identified by nested polymerase chain reaction assays. Compared with related sequences in GenBank, the HBoV-positive strain isolated in the present study was,quite surprisingly, a rare genotype named HBoV4. This strain was a typical HBoV4,with high levels of nucleotide and amino acid homology to the Thailand strain, JQ267789 (98.9% and 98.7%, respectively), and the USA strain, GQ506568 (97.6% and 97.4%, respectively). This is the first report of HBoV4 as the causative agent for acute gastroenteritis in pediatric patients in China. This strain is one of two genotypes of HBoV that are currently circulating.
Child, Preschool ; China ; Feces ; virology ; Female ; Gastroenteritis ; virology ; Human bocavirus ; classification ; genetics ; isolation & purification ; Humans ; Infant ; Male ; Molecular Sequence Data ; Parvoviridae Infections ; virology ; Phylogeny

The chemical components of mainstream smoke were trapped by the artifical salvia. A novel method) for the analysis of the trapped chemical components was established using stir bar sorptive extraction(SBSE)) followed by a thermal desorption-gas chromatography-mass spectrometry(TD-GC-MS). The major) factors including desorption temperature, desorption time, cryofocusing temperature, extraction time and the addition of sodium chloride were studied. Under the optimized conditions, the average relative standard deviation(RSD) of peak areas of 44 components which were identified from the artifical saliva for 6 determinations was less than 10%, which showed the good repeatability of this method. The experiment result indicated that the SBSE-TD-GC-MS technique provided a powerful tool for the analysis of smoke components of some cigarette) trapped and dissolved in the saliva.

Objective To construct a prokaryotic expression system of ahpC gene of Helicobacter pylori. Methods The ahpC gene was amplified from Hp chromosomal DNA by PCR technique and cloned into the expression vector pET-30a. The recombinant vector pET30a-ahpC was identified by DNA sequencing and transformed to E.coli BL21 (DE3) for expression under induction by IPTG. The expression product was analyzed by SDS-PAGE. Results PCR product showed that ahpC gene consisted of 594bp. The gene fragment that was inserted into the recombinant vector was identified to GenBank for 99%. SDS-PAGE showed that the induced protein was expressed highly in the host bacterium. Conclusion A prokaryotic high-expression system for ahpC gene has been successfully constructed. It can highly express r-AhpC protein in E.coli.

Objective To explore the effects of silver needle with systematical on shoulder-hand syndrome (SHS) after stroke. Methods 80 stroke patients with SHS were randomly divided into silver needle group (obsevation group) and body acupuncture group (control group) with 40 cases each group. Both groups received same rountine treatment and rehabilitation exercises after acupuncture. The color Doppler flow imaging (CDFI) was used to measure the velocity of blood flow of the upper limbs, and the Fugl-Meyer Assessment was used to assess motor function of the upper limbs after 3 weeks. Results There was no significant difference between 2 groups before and 1 months after treatment (P>0.05), but there was significant difference 3 and 6 months after treatment (P<0.01). The velocity of blood flow of ulnar artery,radial artery, ulnar veins, radial veins and middle finger digital artery were higher in the observation group than in the control group (P<0.01). Conclusion The silver needle combined with systematical rehabilitation can improve the velocity of blood flow of artery and veins of upper limb, and improve the motor function of upper limb.

Objective To investigate the role of miR-125b on hepatic angiogenesis,with the hope of providing new targets for the prevention and treatment of liver fibrosis.Methods The human hepatic sinusoidal endothelial cells were transfected with miR-125b mimics and inhibitors,and the mRNA and protein expression of vascular endotheli-al growth factor(VEGF),cluster of differentiation antigens 31(CD31),von Willebrand factor(vWF),collagenⅣ,and laminin(LN)were detected by qRT-PCR and ELISA,and the expression of nitric oxide(NO)was detec-ted by fluorescent probe,scanning electron microscopy detected the alteration of the window holes on the surface of human hepatic sinusoidal endothelial cells,angiogenesis assay was performed to observe the neovascularization of each group,and dual luciferase reporter gene assay was performed to validate the targeting relationship between miR-125b and VEGF.Results qRT-PCR and ELISA showed that compared with the negative control group,the mRNA and protein expressions of VEGF,CD31,vWF,Collagen Ⅳ,and LN significantly decreased after miR-125b mimic transfection(P＜0.05),while the mRNA and protein expressions of VEGF,CD31,vWF,CollagenⅣ,and LN were significantly increased after transfection with miR-125 b mimics(P＜0.05);fluorescent probe detection showed that compared with the negative control group,the average fluorescence of intensity expression NO decreased significantly(P＜0.05),while the average fluorescence intensity expression of NO increased significant-ly after miR-125b inhibitor transfection(P＜0.05);the number of fenestrations on the surface of human liver sinu-soidal endothelial cells significantly increased after miR-125b mimic transfection(P＜0.05),while the number of fenestrations on the surface of human liver sinusoidal endothelial cells decreased significantly after miR-125 b inhibi-tor transfection(P＜0.05);angiogenesis assay showed that compared with the negative control group,the number of angiogenesis significantly decreased after miR-125b mimic transfection(P＜0.05),while the number of angio-genesis significantly increased after miR-125b inhibitor transfection(P＜0.05);dual luciferase reporter gene assay showed that compared with negative control group,the expression of relative fluorescence intensity after transfection of miR-125b mimics in VEGF wild-typ significantly decreased(P＜0.05),while the expression of relative fluores-cence intensity after transfection of miR-125b mimics in VEGF mutant significantly decreased(P＞0.05).Con-clusion miR-125b can inhibit liver angiogenesis and thus play an anti-fibrosis role,which can provide a new ref-erence for the prevention and treatment of chronic liver disease and the development of new drugs.

Objective To establish a UPLC-MS/MS method for the determination of dexmedetomidine in human plasma and investigate the effect of obstructive jaundice on pharmacokinetics of dexmedetomidine in vivo. Methods Samples were obtained by liquid-liquid extraction. Agilent Eclipse Plus C₁₈ column was used for chromatograph with methanol and 0.1% formic acid-water solution as mobile phase. Flow rate was 0.2 ml/min. The column temperature was 35 ℃, and the MS detection was selected in MRM mode. Results The calibration curves of dexmedetomidine showed good linearity in the ranges of 0.01−10.00 ng/ml. The results of intra and inter-day precisions were both within 15%. The recovery rate was 85.5%−93.1%. Matrix effect was 91.2%−95.6%. Samples remained stable during analysis. Compared with the control group, c_max、AUC_(0−t)、AUC_(0−∞) and V_z of dexmedetomidine in the patients with obstructive jaundice were increased by 63.4%, 78.9, 66.4%, 82.5%, respectively (P<0.01). CLz was decreased by 42.1%. Conclusion This method is accurate, sensitive and reproducible. It is suitable for dexmedetomidine assay in human plasma. The elimination rate of dexmedetomidine is slower in obstructive jaundice.

【Objective】 To investigate the serum levels of homocysteine (HCY) and cystatin C (Cys-C) in patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV)associated renal injury, and to explore the diagnostic value of combined detection of HCY and Cys-C in ANCA-associated renal injury. 【Methods】 We selected 70 ANCA-positive patients with renal injury admitted to the Department of Nephrology in our hospital from January 2016 to January 2019. Another 48 patients with ANCA-positive but not complicated with renal injury were in simple ANCA positive group while 50 patients with physical examination who came to the hospital during the same period were in control group. Healthy patients served as the control group. By comparing the differences in the test results of different groups, the area under the curve (AUC) of each test index for ANCA-positive renal injury was determined, and the correlation and diagnostic value of combined detection of HCY and Cys-C for ANCA-related renal injury were calculated. 【Results】 The levels of HCY and Cys-C in the ANCA positive combined with renal injury group, the pure ANCA positive group, and the control group differed significantly (P<0.05). The positive rates of HCY and Cys-C in the two groups were significantly different (P<0.05). The sensitivity and specificity of HCY and Cys-C in combination for the diagnosis of ANCA-positive related renal injury were significantly higher than those of the above indicators alone (P<0.05). ROC curve results showed that HCY combined with Cys-C had a better diagnosis for patients with positive ANCA and associated renal injury and a higher correlation. 【Conclusion】 HCY combined with Cys-C is closely correlated to ANCA-related renal injury, and the combined detection has a positive effect on the diagnosis of patients with ANCA-positive related renal injury, and has guiding value for the treatment of patients.

OBJECTIVETo describe the distribution characteristics of outpatient/emergency cases of non-motor road traffic injuries in China from 2006-2013.

METHODSCases of non-motor road traffic injuries were descriptively analyzed based on their data at the sentinel hospitals of the Chinese National Injury Surveillance System (NISS) from 2006 to 2013.

RESULTSThe proportion of non-motor road traffic injuries among the total road traffic injuries of the year from 2006 to 2011 was relatively stable, and the proportion in 2013 was higher than 2012 (χ² = 508.138, P < 0.001). In the non-motor road traffic injuries cases in 2013, male cases were higher than females (χ² = 41.018, P < 0.001), mostly found in 15 to 64 years old age groups. In urban areas, the proportion of non-motor road traffic injuries cases (52.11%) was higher than rural areas (47.89%) (χ² = 88.427, P < 0.001). Most injuries were found in lower limbs (29.08%) and head (24.82%). Injuries were mainly bruises (73.54%) and fractures (10.72%). The severities of injuries were mainly mitigated injury (80.49%), and most injuries ended with home return following treatment (82.34%).

CONCLUSIONThe proportion of non-motor road traffic injuries among total road traffic injuries of the year as found from NISS remained stable from 2006 to 2011, with a slight rise in 2012-2013. Most of the non-motor road traffic injuries were suffered by young and middle aged men. Interventions should be made based on gender and age differences of non-motor road traffic injuries cases.

Accidents, Traffic ; Adolescent ; Adult ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Wounds and Injuries ; epidemiology ; Young Adult