Asthma pathogenesis seems to be a result of a complex mixture of genetic and environmental influences.There is evidence showing that viral and some atypical bacterial play a role in promoting airway inflammation that could contribute to the onset and clinical course of asthma.However,the role of bacterial in asthma is not completely explained.This article is to explain the relationship between airway bacterial infections and asthma and assessed the mechanism of asthma induced by bacterial infection.

Objective To observe the dynamic changes of adrenomedulin(ADM) and calcitonin-gene-relat-ed peptide(CGRP) at different time before,during and after laparoscopic operation for treating CO2 pneumoperitone-urn. Methods 47 cases undergoing laparoscopic operation in our hospital were selected. Plasma ADM and CGRP levels were determined by RIA before anesthesia(T0),10 min before (T1) and 10 min (T2) ,30 min (T3),60 min (T4),and 30 min (TS) after CO2 pneumoperitoneum was treated. Results There was statistical significance in ADM and CGRP level at the different time points before and after CO2 pneumoperitoneum (F=9.686 and 49.804, P<0.01). There was no significance in ADM and CGRP level at TO and T1 (P>0.05);ADM level would decrease at T2 (P<0.05) but CGRP level decrease slowly with no remarkable change as compared with that before CO2 pneumoperitoneum(P>0.05). With time going on, ADM and CGRP level at T3 and T4 was remarkably lower than that at T1 (P<0.01);ADM and CGRP level at T5 would come to that at T1. Conclusions CO2 pneumoperitoneum during laparoscopic operation is the major factor causing decreased ADM and CGRP level;The decrease of ADM and CGRP level will influence the cardiac endocrine function,so CO2 pneumoperitoneum operation should be taken seri-ously when the patients have heart disease and cryptorrhea.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Female ; Humans ; Tremor ; therapy

Objective: To work out a screening checklist for childhood autism and explore its reliability and validity. Methods: Items were selected based on the knowledge of clinical manifestation and diagnostic criteria of childhood autism. The autism diagnostic interview-revised version was used to refine the draft. Three groups (childhood autism, mental retardation and normal control) were used to test the reliability and validity of the new screening tool. Results: The checklist had satisfying reliability including good inter-rater reliability, test-retest reliability, split half reliability and internal consistency. The differential validity of the checklist and the differential diagnostic validity of the items were good, so as the construct validity and the criterion validity. The cut-off point for screening and diagnosis were 21 and 24 respectively. Conclusion: The childhood autism checklist has good reliability and validity, and fit for the situation of Chinese children with the disorder.

Objective To explore the protection effects of the reduced form of nicotinamide-adenine denucleotid(NADH) on cardiac fibroblasts apoptosis induced by hydrazine in vitro.Methods Cardiac fibroblasts isolated from neonate rats were divided into four groups.In NADH pretreatment before hydrazine treatment group,the cardiac fibroblasts were cultured first in the medium containing 400?g/ml NADH for 2 hours,and then cultured in the medium containing 400?g/ml NADH plus 2mmol/L hydrazine for 72 hours;in hydrazine treatment group,the cardiac fibroblasts were cultured first in normal medium containing 400?g/ml NADH for 2 hours and then cultured in the medium containing 2mmol/L hydrazine for 72 hours;in control group,the cardiac fibroblasts were cultured only in normal medium for 72 hours;and in NADH pretreatment control group,the cardiac fibroblasts were cultured in medium containing 400?g/ml NADH for 72 hours.After the treatments mentioned above,the changes in nuclear of apoptotic cardiac fibroblasts as stained by Hoechst33258 were observed under fluorescence microscope,the apoptotic and necrosis rates of cardiac fibroblasts stained by AnnexinⅤ/PI were assayed by flow cytometry,the mitochondria membrane potential of cells stained by rhodamine123 was also assayed by flow cytometry.Results Hydrazine could induce apoptosis of the cardiac fibroblasts.After NADH pretreatment,the number of apoptotic cardiac fibroblasts with pyknosis of nuclear was lowered,the rates of apoptosis and necrosis decreased,and the mitochondria membrane potential of cells was elevated.Conclusion NADH pretreatment could reduce the apoptosis induced by hydrazine by improving the function of mitochondria.

Objective:To explore the diagnostic value of dynamic electrocardiogram (DCG ) in syncope patients . Methods :A total of 148 inpatients and outpatients ,who were diagnosed as syncope in our hospital from 2010 to 2013 ,received 24h DCG examination .Results:Among the 148 patients ,there were 94 cases (63.5% ) with abnor‐mal DCG ,40 cases (27.0% ) occurred syncope during monitoring ,25 cases (16.9% ) occurred syncope‐related ar‐rhythmias ,another one case manifested as atrial fibrillation with rapid ventricular rate during monitoring but didn't appear syncope‐related symptoms such as dizziness and amaurosis etc .,15 patients (10.1% ) occurred syncope‐relat‐ed symptoms during monitoring ,but DCG examination didn't find arrhythmias .Conclusion:Dynamic electrocardio‐gram examination can find syncope‐related arrhythmias ,then they may receive related therapeutic measures for im‐proving prognosis .

Angiosteosis is associated with increased vascular stiffness and leads to clinical manifestations such as high blood pressure and heart failure,which increases the risk of cardiovascular morbidity and mortality.Angiosteosis is considered to be ectopic deposits of calcium phosphate crystallization in the form of hydroxyapatite in cardiovascular tissue.However,the mechanism of angiosteosis is not yet clear.The pathophysiologic process of angiosteosis is similar to normal bone mineralization.Matrix vesicles (MV) play an important role in bone mineralization.Researches confirmed the existence of MV in the calcified arteries under the microscope.Angiosteosis is widely found in clinical diseases,so the studies of the mechanism of angiosteosis and the role of MV in angiosteosis are of great significance.

Aim To evaluate the effects of pioglitazone,a thiazolidinedione on the heart of diabetic animals.Methods Streptozotocin(STZ)-induced diabetic rats were divided into two groups: untreated-DM and treated-DM. Pioglitazone(20 mg?kg~(-1)?d~(-1)) was mixed in water fed to the diabetic treated group.After 5 wk,the left ventricle mass index(LVI),cardiomyocytes size and diameters,the content of hydroxyproline and collagen volume fraction(CVF) were measured.The left myocardium sections were made for HE,MASSON straining to observe the level of myocardial cells' hypertrophy and the accumulation of collagen individually and were examined by light microscope.Myocardial ultrastructure was studied by electronic microscope.Results Compared with untreated-DM group,the LVI,cardiomyocytes size and diameters,the content of hydroxyproline and CVF were lower in treated-DM group(P

AIM To explore possible action mechanism of antidepressants. METHODS Using flow cytometry, the cell proliferation was detected. The proliferation of hippocampal progenitor cells and level of brain derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS Treatment with N methyl D aspartate (NMDA) 600 ?mol?L -1 for 3 d significantly decreased the percentage of S phase in PC12 cells, while in the presence of classical antidepressants, desipramine (DIM) or fluoxetine (FLU) 1, 5 ?mol?L -1 , the percentage of S phase increased. Furthermore, the proliferation of hippocampal progenitor cells, as well as the BDNF level in dentate gyrus (subgranular zone) significantly decreased in chronically stressed mice for 24 d, while chronic administration with DIM or FLU 10 mg?kg -1 (ip) normalized it. Meanwhile, the BDNF level in dentate gyrus also elevated after DIM or FLU treatments. CONCLUSION Up regulation of the hippocampal neurogenesis is the common action mechanism for antidepressants, which may be closely related to the elevation of BDNF level at the same time.

Bioethnopharmaceutical analytical pharmacology (BAP) means to study Chinese herbal compound formula (CHCF) from the aspacts of in vivo and in vitro efficacy,pharmacodynamics,quality control and plant chemistry,guided by the CHCF absorbed bioactive compounds (ABC) analyses.The form of BAP is performed by comparing ABC efficacy with mother formula efficacy.Meanwhile,it must follow the principle which the ABC dose should be equal to the mother formula content or the blood drug concentration.In this study,the hypothesis was put forward to clarify the thoughts,assumptions and expected results,which uncovered the multiple cardioprotective mechanism of Dangui-Buxue Tang (DBT) for ischemic heart disease.BAP is expected to guide the development of further experiments for providing a better thought for the research over CHCF.