Objective To explore the clinical psychological intervention and edaravone in the treatment of acute cerebral infarction.Methods In the period from January 2016 to December 2016, 335 patients were treated with acute cerebral infarction in our hospital, and these patients were randomly divided into two groups: control group and observation group, control group A total of 168 cases, the observation group of patients a total of 167 cases. First, the control group of patients using conventional medical treatment for treatment, the observation group of patients will need in the conventional medical treatment based on the increase in the use of edaravone intravenous infusion. And then the two groups of patients are using clinical psychological intervention care for nursing work. Through the follow-up approach, the two groups of patients to observe the effect of treatment, comparison, need to rely on daily living ability (ADL) and the Chinese Stroke Scale (CSS) two assessment indicators to evaluate the patient's treatment.Results Before treatment, the daily living ability (ADL) and the Chinese stroke scale (CSS) two evaluation indicators score, the difference was not statistically significant; After treatment, the observation group of patients with daily living ability (ADL) and the Chinese stroke scale (CSS) two evaluation indicators were significantly better than the corresponding value of the control group, the difference between the two groups statistically significant (P<0.05). The total effective rate was 94.61％ in the observation group, the total treatment effective rate was 72.02％of control group, the therapeutic effect of the observation group was better than that of the control group (P<0.05).Conclusion Patients with acute cerebral infarction on the basis of the use of conventional medical treatment on the basis of increased use of edaravone intravenous infusion, compared to conventional medical treatment is better, while after treatment for patients with clinical psychological intervention Care for the nursing work, the improvement of the treatment effect has a positive effect, help to improve the patient's daily life ability.

Objective To explore the inhibitory mechanism of Imatinib mesylate on pulmonary fibrosis induced by bleomycin in mice.Methods A total of 120 C57BL/6 mice were randomly divided into four groups,ie, a control group, a model group, a dexamethasone group, and an Imatinib group.The model of pulmonary fibrosis was established by a single intratracheal instillation of bleomycin in the rats.Then dexamethasone or Imatinib were given intraperitoneally respectively.On day 7,14,21 after the treatment, 10 mice of each group were sacrificed respectively.The expressions of TGF-β1 and α-SMA in lung tissue were analyzed by immunohistochemistry.The mRNA expression of TGF-β1 was measured by RT-PCR.Results The expressions of TGF-β1 and α-SMA in lung tissue at each time point were significantly increased in the model group compared with the control group.And the expressions were obviously decreased in the dexamethasone group and the lmatinib group compared with the model group, with no significant differences between the two treatment groups.The expression of TGF-β1 was positively correlated with the α-SMA expression (r = 0.251, P < 0.05).Conclusion The inhibitory effect of Imatinib on pulmonary fibrosis may be related to the inhibition of TGF-β1 and α-SMA expressions.

Objective To discuss the indications for cesarean section in our hospital in recent years.Methods We retrospectively studied the indications for cesarean section of 949 caases in our hospital .Results The average rate of ceasarean section was 34.9%.The order of indications were as following;pregnancy complications(29.6%),relative cephalopelvic monosymmetry(10.6%),breech presentation(10.2%),fetal distress(9.8%),fetal macrosomia(9.2%) and scar uterus(8.9%).The correspondent rate in the diagnosis of fetal macrosomia was 67.5%.The rate of neonatal asphyxia was 6.7%.Conclusion Obstetric medical staff should improve their opinion on the cesarean section and master the indications appropriately.

Objective To analyze clinical Value of serum homocysteine(Hcy)and cystatin C(Cys C) in patients with cerebral in‐farction .Methods From August 2010 to December 2014 ,our hospital 118 patients with cerebral infarction and 62 healthy subjects for the study were divided into the control group and the observation group .The observation group was further divided into light , medium and heavy groups .Test results for the two groups were related to the study of serum homocysteine and Cys C were ana‐lyzed separately and compared between two groups of Hcy levels Cys C contact with cerebral infarction .Results Serum Hcy ,Cys C levels significantly higher in the observation group than that of control group ,differences were statistically significant(P<0 .05);The serum Hcy ,Cys C of patients with severe cerebral infarction group were significantly higher than that of light and medium cer‐ebral infarction group;Serum Hcy ,Cys C levels of patients with medium cerebral infarction group were also significantly higher than the light cerebral infarction group ,differences were statistically significant(P<0 .05) .Conclusion The serum Hcy and Cys C has important clinical value of detecting the disease assessment and treatment of patients with cerebral infarction .

Objective To observe the dynamic changes of adrenomedulin(ADM) and calcitonin-gene-relat-ed peptide(CGRP) at different time before,during and after laparoscopic operation for treating CO2 pneumoperitone-urn. Methods 47 cases undergoing laparoscopic operation in our hospital were selected. Plasma ADM and CGRP levels were determined by RIA before anesthesia(T0),10 min before (T1) and 10 min (T2) ,30 min (T3),60 min (T4),and 30 min (TS) after CO2 pneumoperitoneum was treated. Results There was statistical significance in ADM and CGRP level at the different time points before and after CO2 pneumoperitoneum (F=9.686 and 49.804, P<0.01). There was no significance in ADM and CGRP level at TO and T1 (P>0.05);ADM level would decrease at T2 (P<0.05) but CGRP level decrease slowly with no remarkable change as compared with that before CO2 pneumoperitoneum(P>0.05). With time going on, ADM and CGRP level at T3 and T4 was remarkably lower than that at T1 (P<0.01);ADM and CGRP level at T5 would come to that at T1. Conclusions CO2 pneumoperitoneum during laparoscopic operation is the major factor causing decreased ADM and CGRP level;The decrease of ADM and CGRP level will influence the cardiac endocrine function,so CO2 pneumoperitoneum operation should be taken seri-ously when the patients have heart disease and cryptorrhea.

Objective To observe the effect of glutathione on bleomycin-induced pulmonary fibrosis in rats .Methods Fifty-four SD rats in good conditions were divided into three groups at random:control group ,BLM group,glutathione(GSH) group.On experimental day(day 0),the rats were intratracheally instilled with bleomycin(5mg?kg -1 .body weight),and then treated with intraperitoneal injection of glutathione .On days 7,14,28 after instillation ,six rats of each group were sacrified respectively and the lungs were harvested for histopathological examination and determinations of GSH and hydroxyproline(HYP).Results Pathological findings of GSH group were the same as BLM group,but compared with BLM group,pathomorphologic changes in GSH group was a small extent.The content of GSH in lung tissue was increased significantly(P0 05).Conclusions Glutathione has anti-oxidation and may protect lung tissue free from oxidation injury, but it has no protecting effect on collagen deposition caused by bleomycin.

Cell culture technology is the most commonly used method in the in vitro experiments at present. However, monolayer cell culture technology has been unable to meet the demand of the researchers. This is because that monolayer cell culture cannot mimic the cellular environment in which multiple cells interact with each other in the body. We cannot discuss the relationship of many cells, because we do not know the relationship between cells through a single kind of cell. So cell co-culture medicine arises at the historic moment for the demand. With the development of research method in recent years, cell co-culture method also has been improved in practice: from direct contact co-cultures to indirect contact co-cultures, from two-dimensional co-cultures to three-dimensional co-cultures. Cell co-culture method is closer to the human body. It is also more advantageous to study the interaction among cells. Nowadays, there are more researchers tend to select this method to study the physiological and pathological in vitro model, tissue engineering, and cell differentiation research. At the same time, it has become the focus of drug research and development, drug analysis, mechanism of drug action, and drug targets. This article will review the studies of cell co-culture method, summarize advantages and disadvantages of various methods, so as to promote improvement of cell culture methods, to build cells co-culture system that more close to human body, and build the in vitro model that simulate internal circulation of human body further.

Objective To explore the protection effects of the reduced form of nicotinamide-adenine denucleotid(NADH) on cardiac fibroblasts apoptosis induced by hydrazine in vitro.Methods Cardiac fibroblasts isolated from neonate rats were divided into four groups.In NADH pretreatment before hydrazine treatment group,the cardiac fibroblasts were cultured first in the medium containing 400?g/ml NADH for 2 hours,and then cultured in the medium containing 400?g/ml NADH plus 2mmol/L hydrazine for 72 hours;in hydrazine treatment group,the cardiac fibroblasts were cultured first in normal medium containing 400?g/ml NADH for 2 hours and then cultured in the medium containing 2mmol/L hydrazine for 72 hours;in control group,the cardiac fibroblasts were cultured only in normal medium for 72 hours;and in NADH pretreatment control group,the cardiac fibroblasts were cultured in medium containing 400?g/ml NADH for 72 hours.After the treatments mentioned above,the changes in nuclear of apoptotic cardiac fibroblasts as stained by Hoechst33258 were observed under fluorescence microscope,the apoptotic and necrosis rates of cardiac fibroblasts stained by AnnexinⅤ/PI were assayed by flow cytometry,the mitochondria membrane potential of cells stained by rhodamine123 was also assayed by flow cytometry.Results Hydrazine could induce apoptosis of the cardiac fibroblasts.After NADH pretreatment,the number of apoptotic cardiac fibroblasts with pyknosis of nuclear was lowered,the rates of apoptosis and necrosis decreased,and the mitochondria membrane potential of cells was elevated.Conclusion NADH pretreatment could reduce the apoptosis induced by hydrazine by improving the function of mitochondria.

Objective To find out the present situation of complementary feeding, breast-feeding and children nutrition, and further explore the relationship between them. Methods Random sampling was adopted and 7302pairs of mothers and their children under 36 months were investigated by means of questionnaires. Results Breastfeeding rate was 96.8% ,but exclusive breast-feeding rate was only 37.6% . Breast-feeding rate over 12 months was 34.7% ,breast-feeding rate over 24 months was only 15.8%. Timely sucking rate was 8.4％ ,19.2% of children had too early complementary feeding, 26.8% had too late,and 43. 1% had appropriate feeding . To the children over 6months, the feeding rates of meat and eggs per week were 36. 3% and 49. 3 % , respectively. The stunting-rate was 24.0％, underweight-rate 22. 1 ％ ,and wasting-rate 8. 9 ％. The incidence rate of iron deficiency anemia (IDA) was 23.3 %. Multivariable analysis showed that growth of children was associated with diet diversity,intake frequency of eggs and meat, too early complementary feeding,prolonged breast-feeding and diarrhea, etc. Conclusion In rural areas of western China ,breast-feeding has been fundamentally popularized ,however ,the unfit complementary feeding and malnutrition of children under 36 months are common and serious ,important complementary feeding recommendations and good hygiene condition are strongly needed.

Objective The research is to understand students' mobile learning (M-learning) attitudes and behaviors,and provide a reference for the development of mobile learning systems and learning resources.Methods The research used the method of questionnaires to investigate nursing students' present situation of M-learning.Results About 2/3 of the students were so eager to have targeted mobile tutorial course.Most of the students were not satisfied though there were a lot of resources.More than 2/3 of the students used telephone to surf on the internet almost every day (mainly on reading web pages and communicate with classmates using chat software,see / write blog lowest).The mobile learning time of more than 60％ students was not more than 10 minutes.1/3 of students used the time between 10 to 30 minutes.The highest cost that students spent on monthly mobile learning was less than 10 Yuan,accounting for the total number of 1/3.Compared with the junior college students,the proportion of undergraduates who support to use phone to surf on the internet was higher.Undergraduate students were more likely to read pages and see multimedia,most of them hope to see courseware within 2 to 5 minutes,etc.Conclusions Nursing students have good hardware conditions on mobile learning.Students are recognized on mobile learning.The current mobile learning resources are chaos and lack of design so that it hindered the development of mobile learning.Mobile learning courses should be short and pithy,rich and colorful.Application design of mobile blog in mobile learning needs to be strengthened.Generally speaking,undergraduate college students are more actively to take mobile learning into their learning and life.